Two studies have analysed the half-lives of long non-coding RNAs (lncRNAs). Clark et al. used a custom non-coding RNA array to analyse the half-lives of ~800 lncRNAs and ~12,000 mRNAs in the mouse neuro-2a cell line. Tani et al. developed a new technique called 5′-bromo-uridine immunoprecipitation chase followed by deep sequencing (BRIC–seq), in which RNAs are labelled by pulse chasing with 5′-bromo-uridine, to survey RNA half-life in HeLa cells. Both groups find that lncRNA half-lives vary over a wide range that is comparable with that of mRNAs; longer half-lives may be indicative of functionality in lncRNAs.
ORIGINAL RESEARCH PAPERS
Tani, H. et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 27 Feb 2012 (doi:10.1101/gr.130559.111)
Clark, M. B. et al. Genome-wide analysis of long noncoding RNA stability. Genome Res. 9 March 2012 (doi:10.1101/gr.131037.111)
Rights and permissions
About this article
Cite this article
Stower, H. Long non-coding RNA stability. Nat Rev Genet 13, 298 (2012). https://doi.org/10.1038/nrg3234
Published:
Issue Date:
DOI: https://doi.org/10.1038/nrg3234
