This paper describes a high-throughput method for quantifying gene expression in non-model organisms. The new approach — EcoP151-tagged digital gene expression (EDGE) — uses a unique tag for each expressed transcript, levels of which are determined from the number of high-throughput sequencing reads for each tag. This method avoids the need for a high-quality reference genome, as required for RNA sequencing (RNA-seq). A validation study showed that EDGE compares favourably to RNA-seq in terms of measuring transcript abundance.
ORIGINAL RESEARCH PAPER
Hong, L. Z. et al. Digital gene expression for non-model organisms. Genome Res. 15 Aug 2011 (doi:10.1101/gr.122135.111)
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Flintoft, L. Measuring gene expression in non-model organisms. Nat Rev Genet 12, 742 (2011). https://doi.org/10.1038/nrg3105
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DOI: https://doi.org/10.1038/nrg3105