Next-generation sequencing

Definition

Next-generation sequencing refers to non-Sanger-based high-throughput DNA sequencing technologies. Millions or billions of DNA strands can be sequenced in parallel, yielding substantially more throughput and minimizing the need for the fragment-cloning methods that are often used in Sanger sequencing of genomes.

Latest Research and Reviews

  • Research | | open

    Amyotrophic lateral sclerosis (ALS) is a rapidly progressing neurodegenerative disease. Here, Wray and colleagues identify association of the GPX3-TNIP1 locus with ALS using cross-ethnic meta-analyses.

    • Beben Benyamin
    • , Ji He
    • , Qiongyi Zhao
    • , Jacob Gratten
    • , Fleur Garton
    • , Paul J. Leo
    • , Zhijun Liu
    • , Marie Mangelsdorf
    • , Ammar Al-Chalabi
    • , Lisa Anderson
    • , Timothy J. Butler
    • , Lu Chen
    • , Xiang-Ding Chen
    • , Katie Cremin
    • , Hong-Weng Deng
    • , Matthew Devine
    • , Janette Edson
    • , Jennifer A. Fifita
    • , Sarah Furlong
    • , Ying-Ying Han
    • , Jessica Harris
    • , Anjali K. Henders
    • , Rosalind L. Jeffree
    • , Zi-Bing Jin
    • , Zhongshan Li
    • , Ting Li
    • , Mengmeng Li
    • , Yong Lin
    • , Xiaolu Liu
    • , Mhairi Marshall
    • , Emily P. McCann
    • , Bryan J. Mowry
    • , Shyuan T. Ngo
    • , Roger Pamphlett
    • , Shu Ran
    • , David C. Reutens
    • , Dominic B. Rowe
    • , Perminder Sachdev
    • , Sonia Shah
    • , Sharon Song
    • , Li-Jun Tan
    • , Lu Tang
    • , Leonard H. Berg
    • , Wouter Rheenen
    • , Jan H. Veldink
    • , Robyn H. Wallace
    • , Lawrie Wheeler
    • , Kelly L. Williams
    • , Jinyu Wu
    • , Xin Wu
    • , Jian Yang
    • , Weihua Yue
    • , Zong-Hong Zhang
    • , Dai Zhang
    • , Peter G. Noakes
    • , Ian P. Blair
    • , Robert D. Henderson
    • , Pamela A. McCombe
    • , Peter M. Visscher
    • , Huji Xu
    • , Perry F. Bartlett
    • , Matthew A. Brown
    • , Naomi R. Wray
    •  & Dongsheng Fan
  • Research | | open

    Draft genome, 994 re-sequenced lines and GWAS for yield-traits provide a resource of genetics and genomics tools for pearl millet researchers and breeders.

    • Rajeev K Varshney
    • , Chengcheng Shi
    • , Mahendar Thudi
    • , Cedric Mariac
    • , Jason Wallace
    • , Peng Qi
    • , He Zhang
    • , Yusheng Zhao
    • , Xiyin Wang
    • , Abhishek Rathore
    • , Rakesh K Srivastava
    • , Annapurna Chitikineni
    • , Guangyi Fan
    • , Prasad Bajaj
    • , Somashekhar Punnuri
    • , S K Gupta
    • , Hao Wang
    • , Yong Jiang
    • , Marie Couderc
    • , Mohan A V S K Katta
    • , Dev R Paudel
    • , K D Mungra
    • , Wenbin Chen
    • , Karen R Harris-Shultz
    • , Vanika Garg
    • , Neetin Desai
    • , Dadakhalandar Doddamani
    • , Ndjido Ardo Kane
    • , Joann A Conner
    • , Arindam Ghatak
    • , Palak Chaturvedi
    • , Sabarinath Subramaniam
    • , Om Parkash Yadav
    • , Cécile Berthouly-Salazar
    • , Falalou Hamidou
    • , Jianping Wang
    • , Xinming Liang
    • , Jérémy Clotault
    • , Hari D Upadhyaya
    • , Philippe Cubry
    • , Bénédicte Rhoné
    • , Mame Codou Gueye
    • , Ramanjulu Sunkar
    • , Christian Dupuy
    • , Francesca Sparvoli
    • , Shifeng Cheng
    • , R S Mahala
    • , Bharat Singh
    • , Rattan S Yadav
    • , Eric Lyons
    • , Swapan K Datta
    • , C Tom Hash
    • , Katrien M Devos
    • , Edward Buckler
    • , Jeffrey L Bennetzen
    • , Andrew H Paterson
    • , Peggy Ozias-Akins
    • , Stefania Grando
    • , Jun Wang
    • , Trilochan Mohapatra
    • , Wolfram Weckwerth
    • , Jochen C Reif
    • , Xin Liu
    • , Yves Vigouroux
    •  & Xun Xu
  • Protocols |

    Knockout Sudoku allows construction of whole-genome knockout collections for a wide range of microorganisms at a lower cost and increased speed, using combinatorial pooling, next-generation sequencing, and a Bayesian inference algorithm to process and annotate extremely large progenitor transposon insertion mutant collections.

    • Isao A Anzai
    • , Lev Shaket
    • , Oluwakemi Adesina
    • , Michael Baym
    •  & Buz Barstow
    Nature Protocols 12, 2110–2137
  • Reviews |

    Ancient genomes can inform our understanding of the history of human adaptation through the direct tracking of changes in genetic variant frequency across different geographical locations and time periods. The authors review recent ancient DNA analyses of human, archaic hominin, pathogen, and domesticated animal and plant genomes, as well as the insights gained regarding past human evolution and behaviour.

    • Stephanie Marciniak
    •  & George H. Perry

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