We thank Barber-Chamoux and Esler for their Reply (Platelet noradrenaline uptake is unrelated to renal denervation. Nat. Rev. Cardiol. doi:10.1038/nrcardio.2016.144)1 to our Correspondence (Renal denervation for resistant hypertension: the wrong target? Nat. Rev. Cardiol. 13, 388; 2016)2, in which we argued why the current theoretical basis for renal denervation therapy — as described in the Perspectives article by Gulati et al. (Opinion: The rise, fall, and possible resurrection of renal denervation. Nat. Rev. Cardiol. 13, 238–244; 2016)3 — might be flawed. Barber-Chamoux and Esler raised several issues regarding our hypothesis that platelets are responsible for increased noradrenaline spillover in hypertension1. Before responding to these arguments, we would like briefly to outline the process of noradrenaline metabolism.
Noradrenaline metabolism can be divided into three stages: neuronal release–reuptake (upstream), blood cell-mediated clearance (midstream), and organ-mediated degradation and excretion (downstream)4 (Fig. 1). Indeed, increasing sympathetic nerve firing increases noradrenaline spillover. In the midstream stage, noradrenaline is degraded or inactivated by catechol-O-methyltransferase, phenolsulfotransferase, and monoamine oxidase in blood cells, and simultaneously concentrated and stored in platelets. Platelets serve as 'containers' to transport noradrenaline to downstream detoxifying/excretory organs for degradation and excretion. Each of the organs involved contributes to the total noradrenaline clearance rate (for example, renal insufficiency decreases noradrenaline clearance)4. Clearly, midstream and downstream noradrenaline metabolism, although often neglected or underestimated, is also a determinant of noradrenaline spillover.
The role of neuronal uptake emphasized by Barber-Chamoux and Esler1 is highly questionable, because evidence for neuronal uptake of plasma noradrenaline came solely from pharmacological studies using noradrenaline-uptake inhibitors, especially desipramine (desmethylimipramine)5, which are known also to inhibit platelet uptake. Inhibition of platelet uptake has two effects: increasing plasma noradrenaline level and decreasing platelet noradrenaline release during platelet destruction. These effects might lead to regional differences in noradrenaline spillover in response to desipramine, owing to differences in platelet destruction between organs, with the heart having the lowest platelet-destruction rate6. This discrepancy can explain why intravenous desipramine infusion increases cardiac noradrenaline spillover, but decreases renal noradrenaline spillover7.
With regard to platelet noradrenaline uptake, in vitro evidence shows that platelets rapidly take up noradrenaline and convert it to a sulfated form8. Platelet noradrenaline-degrading enzymes help to maintain platelet noradrenaline at a stable level unless they are saturated by increased plasma noradrenaline (for example, in pheochromocytoma and hypertension). The finding that hypertension onset occurs following an increase in mean platelet volume4 suggests the importance of platelets in lowering plasma noradrenaline and blood pressure. Unfortunately, the dynamic contribution of platelet noradrenaline- degrading enzymes was not taken into account in previous studies on the relationship between plasma and platelet noradrenaline levels, leading to inaccurate explanations on platelet neurotransmitter flux.
We disagree with the argument (which is based only on platelet pool size) that platelets do not contribute substantially to plasma noradrenaline clearance. Besides renal disease, cirrhosis is also well-documented to increase both mean platelet volume9 and noradrenaline spillover5. Given that renal disease increases platelet destruction (renal thrombotic microangiopathy) and that the liver is a major platelet-destroying organ6, a plausible explanation for these phenomena is that the noradrenaline concentrated in platelets cannot be effectively eliminated during intraorgan platelet destruction, consequently leading to increased noradrenaline spillover.
In conclusion, platelet overload might be a major cause of increased noradrenaline spillover in hypertension, reflecting detoxifying/excretory insufficiency that cannot meet the needs of the sympathetic system10. The arguments by Barber-Chamoux and Esler1 were based on an incomplete understanding of platelet function.
References
Barber-Chamoux, N. & Esler, M. D. Platelet norepinephrine uptake is unrelated to renal denervation. Nat. Rev. Cardiol. http://dx.doi.org/nrcardio.2016.144 (2016).
Zhou, S. S. & Zhou, Y. Renal denervation for resistant hypertension: the wrong target? Nat. Rev. Cardiol. 13, 388 (2016).
Gulati, R. et al. The rise, fall, and possible resurrection of renal denervation. Nat. Rev. Cardiol. 13, 238–244 (2016).
Zhou, S. S., Zhou, Y. M., Li, D. & Chen, N. N. Preeclampsia and future cardiovascular risk: a point of view from the clearance of plasma vasoactive amines. Hypertens. Pregnancy 35, 1–14 (2016).
Esler, M. et al. Overflow of catecholamine neurotransmitters to the circulation: source, fate, and functions. Physiol. Rev. 70, 963–985 (1990).
Heyns, A. D. et al. Kinetics, distribution and sites of destruction of 111Indium-labelled human platelets. Br. J. Haematol. 44, 269–280 (1980).
Esler, M. D. et al. Effects of desipramine on sympathetic nerve firing and norepinephrine spillover to plasma in humans. Am. J. Physiol. 260, R817–R823 (1991).
Mielke, K. & Strobel, G. The potential of intact human platelets for sulfoconjugation of norepinephrine in vitro. Life Sci. 55, 1657–1663 (1994).
Girleanu, I. et al. The risk of thrombotic events in patients with liver cirrhosis. Rev. Med. Chir. Soc. Med. Nat. Iasi 116, 991–996 (2012).
Zhou, S. S. & Zhou, Y. Renal sympathetic hyperactivity in hypertension: true or false? Circ. Res. 119, e33–e34 (2016).
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Zhou, SS., Zhou, Y. Platelet noradrenaline overload — cause of increased noradrenaline spillover in hypertension?. Nat Rev Cardiol 13, 694 (2016). https://doi.org/10.1038/nrcardio.2016.145
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DOI: https://doi.org/10.1038/nrcardio.2016.145