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Deep resequencing and association analysis of schizophrenia candidate genes

Molecular Psychiatry volume 18, pages 138–140 (2013)Cite this article

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In 2005, we selected 10 genes for which there was reasonable evidence for involvement in the etiology of schizophrenia (COMT, DAOA, DISC1, DRD2, DRD3, DTNBP1, HTR2A, NRG1, SLC6A3 and SLC6A4, Supplementary Table S1).1 Although these genes have not received support from far larger and comprehensive subsequent studies, and may not contain common etiological variations,2 it is possible that they contain uncommon variations of etiological importance. To test this hypothesis, we conducted a multistage resequencing study.

In stage 1, we used Sanger methods to sequence the exons, 5′ and 3′ untranslated regions, splice sites, promoters and conserved intronic regions of these 10 genes in 727 cases with schizophrenia from CATIE3 and 733 controls of European (EUR) and African (AFR) ancestry. In stage 2, we validated single-nucleotide variants (SNVs) using Roche 454 sequencing in the same samples. In stage 3, we genotyped prioritized SNVs in independent samples (Supplementary Material and Supplementary Figure S1).

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Authors and Affiliations

  1. Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    J J Crowley, C E Hilliard, Y Kim & P F Sullivan

  2. Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA

    M B Morgan, L R Lewis, D M Muzny, A C Hawes, A Sabo, D A Wheeler & R A Gibbs

  3. Department of Psychiatry, Columbia University, New York, NY, USA

    J A Lieberman

Authors
  1. J J Crowley
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  2. C E Hilliard
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  3. Y Kim
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  4. M B Morgan
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  5. L R Lewis
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  6. D M Muzny
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  7. A C Hawes
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  8. A Sabo
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  9. D A Wheeler
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  10. J A Lieberman
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  11. P F Sullivan
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  12. R A Gibbs
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Corresponding author

Correspondence to J J Crowley.

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The authors declare no conflict of interest.

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Supplementary Information accompanies the paper on the Molecular Psychiatry website

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Crowley, J., Hilliard, C., Kim, Y. et al. Deep resequencing and association analysis of schizophrenia candidate genes. Mol Psychiatry 18, 138–140 (2013). https://doi.org/10.1038/mp.2012.28

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