DNA sequencing is any chemical, enzymatic or technological procedure for determining the linear order of nucleotide bases in DNA. Sanger sequencing by replicative synthesis in the presence of dideoxy nucleotide chain terminator monomers has now given way to 'next generation' sequencing by short parallel read technologies. The term often applies to the entire sequence determination pipeline including post-sequencing software analysis.
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Correspondence |Nature Methods 14, 547–548