Abstract
Glomerulosclerosis is a common pathological finding that often progresses to renal failure. The mechanisms of chronic kidney disease progression are not well defined, but may include activation of numerous vasoactive and inflammatory pathways. We hypothesized that podocytes are susceptible to filtered plasma components, including hormones and growth factors that stimulate signaling pathways leading to glomerulosclerosis. Gα12 couples to numerous G-protein-coupled receptors (GPCRs) and regulates multiple epithelial responses, including proliferation, apoptosis, permeability and the actin cytoskeleton. Herein, we report that genetic activation of Gα12 in podocytes leads to time-dependent increases in proteinuria and glomerulosclerosis. To mimic activation of Gα12 pathways, constitutively active Gα12 (QL) was conditionally expressed in podocytes using Nphs2-Cre and LacZ/floxed QLα12 transgenic mice. Some QLα12LacZ+/Cre+ mice developed proteinuria at 4–6 months, and most were proteinuric by 12 months. Proteinuria increased with age, and by 12–14 months, many demonstrated glomerulosclerosis with ultrastructural changes, including foot process fusion and both mesangial and subendothelial deposits. QLα12LacZ+/Cre+ mice showed no changes in podocyte number, apoptosis, proliferation or Rho/Src activation. Real-time PCR revealed no significant changes in Nphs1, Nphs2, Cd2ap or Trpc6 expression, but Col4a2 message was increased in younger and older mice, while Col4a5 was decreased in older mice. Confocal microscopy revealed disordered collagen IVα1/2 staining in older mice and loss of α5 without changes in other collagen IV subunits. Taken together, these studies suggest that Gα12 activation promotes glomerular injury without podocyte depletion through a novel mechanism regulating collagen (α)IV expression, and supports the notion that glomerular damage may accrue through persistent GPCR activation in podocytes.
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Main
Glomerulosclerosis (GS) is a common pathological finding in patients with progressive chronic kidney disease (CKD) and often leads to end-stage renal disease. Numerous conditions predispose patients to GS, including diabetes, hypertension, IgA nephropathy, FSGS (focal segmental GS) and immune-mediated injury. In adults over 60 years old, the prevalence of CKD Stage III (glomerular filtration rate, 30–59 ml/min) is estimated to be >25%.1 Although risk factors such as hypertension and diabetes are linked to CKD, little is known about the signaling mechanisms that lead to progression with aging. Post-mortem and nephrectomy samples in otherwise ‘healthy’ adults reveal variable amounts of GS and interstitial fibrosis, suggestive of age-associated damage.2, 3 Recent studies show that primary podocyte injury is sufficient to induce GS.4, 5 Podocytes are exposed to filtered reactive oxygen species (ROS), lipid mediators, cytokines and hormones that could contribute to injury. Many of these molecules activate G-protein-coupled receptors (GPCRs), which couple to multiple Gα subunits. Each of the 16 Gα subunits (four main families: Gαs, Gαi/o, Gαq and Gα12/13) couples to many different GPCRs;6 thus, defining specific pathways in vivo has been difficult.
Gα12/13 are expressed in podocytes and couple to angiotensin II, thrombin, endothelin and LPA receptors, which are important in renal injury.7 Gα12/13 can activate Rho or Src to regulate the actin cytoskeleton,8 in addition to proliferation, transformation,9 tight junction assembly,10, 11, 12 cell–cell adhesion,13, 14 directed cell migration,15 apoptosis16 and cell attachment.17 RhoGDIα knockout mice develop proteinuria and renal failure,18 and many mutations in hereditary FSGS affect proteins linked to the actin cytoskeleton (reviewed in ref. 19). Gα12 also upregulates TGFβ,20, 21 and several gene profiling studies found upregulated Gα12 in proteinuric kidneys and post-transplant CKD (via Nephromine22, 23).
Targeting activated Gα subunits to specific cells in vivo permits identification of downstream effector pathways independent of receptor activation, and thus permits insight into disease mechanisms otherwise impossible to study in vivo. Herein, we confirm the expression of endogenous Gα12 in the major podocyte processes. Constitutively activated Gα12 (QLα12) was expressed in podocytes using a transgenic model that results in mosaic expression and mimics the focal nature of GS pathology. QLα12LacZ+/Cre+ mice develop proteinuria and focal GS without differences in podocyte number, apoptosis, proliferation or Rho/Src signaling over time. Col4a was dysregulated and correlated with altered localization and ultrastructural changes. These findings indicate that Gα12 activation in podocytes leads to dysregulated collagen α(IV) expression and support a model of altered glomerular structure and function resulting from time-dependent stimulation of GPCR-Gα12 signaling pathways.
MATERIALS AND METHODS
Transgenic Mice Creation
All animal procedures were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee at Harvard University. Gα12 (Q229L) EE tagged was cloned into the CMV-floxed LacZ cassette kindly provided by Dr Larry Holzman.24 C57/B6 mice were injected at the Brigham and Women's Hospital Transgenic Mouse Facility and were then crossed with Nphs2/Cre mice on the same C57/BL6 background.
Urine Microalbumin, Serum Creatinine, LPS and Tissue Harvesting
Male and female mice were analyzed for urine microalbumin/creatinine ratio at specified ages using Bayer DCA 200+ Analyzer with software version E3.11/01.04. Mice were defined as proteinuric when microalbumin/creatinine ratio was ≥34 owing to the detection limits of the analyzer. LPS (Invivogen, San Diego, CA, USA) (10 μg/g, intraperitoneally) was administered to 2- to 6-month-old mice, and urine was collected 18 h post-injection and microalbumin/creatinine ratio was determined. Serum was collected and BUN and creatinine were analyzed using an iStat System with CHEM8+ cartridges. Mice were anesthetized using inhaled isofluorane (Phoenix Pharmaceuticals, St Louis, MO, USA). For whole kidney collection, organs were perfused with cold PBS. Kidneys were removed and processed according to methods below.
GST-TPR Pull-Down Assay
The GST-TPR construct was kindly provided by Dr M Negishi (Kyoto University, Kyoto, Japan). GST-TPR was prepared as described previously.16 Harvested kidneys were homogenized in lysis buffer with eComplete (Roche, Indianapolis, IN, USA) protease inhibitors, lysed through 22 G needle and normalized for protein concentration. GST-TPR (1 μg) coupled to glutathione-agarose beads (Amersham Biosciences) was added to 800 μg of total protein and rocked overnight at 4°C. Beads were pelleted with low-speed centrifugation and washed three times with PBS, 0.1% Triton X-100, resuspended in Laemmli sample buffer and analyzed by SDS-PAGE and western blot using a Gα12 antibody (Santa Cruz, Santa Cruz, CA, USA).
Immunogold Electron Microscopy
Immunogold labeling and electron microscopy (EM) was performed at the Membrane Biology Program, Massachusetts General Hospital. Normal mouse kidney was fixed in 2% freshly made paraformaldehyde and 0.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) and processed according to standard conditions. Grids were incubated with rabbit anti-Gα12 (Santa Cruz) overnight at 4°C, followed by a 25 μl droplet of anti-goat IgG conjugated with gold (10 nm). Grids were washed and imaged using Philips CM10 electron microscope (Philips Electronics, Mahwah, NJ, USA).
Histology
Fixed kidney tissue was paraffin processed and 4 μm sections were stained with PAS. Light microscopic evaluation included quantification of the total number of glomeruli present in one microscopic section as well as quantification of glomeruli with lesions (global sclerosis, segmental sclerosis, glomerular collapse and glomerular hypertrophy).
Electron Microscopy
Fixed specimens were trimmed, post-fixed with osmium tetroxide, dehydrated in serial ethanols and embedded in epoxy resin. Ultrathin sections were cut at 80 nm, mounted on 200 mesh copper grids, treated with uranyl acetate and lead citrate, and examined in a JEOL 1010 transmission electron microscope (Tokyo, Japan). Electron micrographs showing glomerular ultrastructure were analyzed in a blinded manner. Podocytes, endothelium, glomerular basement membrane (GBM) and mesangium were examined and scored from 0 (no abnormality) to 4 (severe abnormality). Podocytes were scored for foot process (FP) effacement and irregularity, microvillous degeneration, vacuolization and subepithelial deposits. Endothelium was scored for: double contours, subendothelial deposits and swelling. GBM abnormalities were scored for thickness and irregularity, and the mesangium was scored for deposits, expansion by matrix and cellular expansion.
LacZ, TUNEL and Immunostaining
Fixed kidneys were washed in PBS and rehydrated overnight in 30% sucrose. Kidneys were embedded in OCT Compound (TissueTek, Sakura Finetek, Torrence, CA, USA) and frozen in liquid nitrogen. For Wilms's tumor-1 (WT-1) and TUNEL staining, sections were labeled using the ‘In Situ Cell Death Detection Kit’ (Roche) according to the manufacturer's protocol. Kidneys were incubated in methanol, blocked and incubated in anti-WT-1 antibody (sc-192; Santa Cruz) at 4°C overnight. Sections were washed, incubated with appropriate secondary antibody and mounted using Prolong Gold (Invitrogen, Carlsbad, CA, USA). Kidneys were photographed on a Nikon E-1000 equipped with a SPOT digital camera. For collagen staining, kidneys were blocked in 1% BSA and incubated with primary antibodies (generous gift of DB Borga, Vanderbilt, Nashville, TN, USA) or (Rockland Gilbertsville, PA, USA). All sections were incubated with appropriate secondary antibody for 1 h at room temperature, and mounted using Prolong Gold (Invitrogen). Images were taken on a Nikon C1 D-Eclipse confocal microscope.
Glomerular Isolation
Glomerular isolation was performed using magnetic beads.25 A measure of 200 μl Dynabeads (M450 Tosylactivated; Invitrogen) were washed with 0.1% PBS and incubated overnight in 0.2 M Tris (pH 8.5) with 0.1% BSA. Beads were washed and resuspended in 30 ml HBSS. Mice were anesthetized and organs perfused with HBSS. Kidneys were digested in 1 mg/ml collagenase A (Roche) and 100 U/ml DNaseI (New England Biolabs, Ipswich, MA, USA) at 37°C for 30 min. Digests were strained though a 100 μm cell strainer, centrifuged and resuspended in HBSS. Glomeruli were obtained using magnetic separator (New England Bio Labs).
RhoA Activation ELISA and Western Blot
Isolated glomeruli were resuspended in lysis buffer with eComplete (Roche) protease inhibitors and lysed through 22 G needle. Rho activity was determined using G-LISA™ RhoA Activation Assay Kit (Cytoskeleton, Denver, CO, USA) and absorbance at 490 nm of an HRP-active RhoA antibody. In addition, samples were analyzed by SDS-PAGE and western blot analysis using an anti-RhoA antibody (Cytoskeleton) as described previously.17
Real-Time PCR
Purified glomeruli were lysed in TRIzol reagent (Invitrogen). RNA concentrations were determined, and RNA was incubated in DNAseI (New England Biolabs) with RNAse inhibitor (Roche). DNAse-treated RNA was reverse transcribed using Transcriptor Reverse Transcriptase (Roche). Negative control without enzyme was included in each analysis. The cDNA template was treated with RNaseH (Invitrogen). TaqMan Gene Expression assays (Applied Biosystems, Foster City, CA, USA) were performed using an ABI 7300 (Applied Biosystems) with the following conditions: 2 min at 50°C and 10 min at 95°C, followed by 50 cycles of 95°C for 15 s and 60°C for 1 min. Melting-curve analysis and gel electrophoresis of PCR products verified that a single product of the expected size was generated with each primer set. Data analysis used the ΔΔCt method. Ct was normalized to 18S ribosomal subunit.
Statistical Analyses
Data are expressed as medians or means±s.e.m. as indicated. Statistical analysis was performed using Prism 4 for Macintosh (GraphPad, La Jolla, CA, USA) using two-way ANOVA, followed by Bonferroni's post-hoc test. Statistical significance was identified at P<0.05.
RESULTS
Gα12 is Expressed in the Glomeruli
Although Gα12 is expressed in proximal and distal tubular epithelial cells26 and glomerular proteomics identified Gα12,27 its localization in the glomeruli has not been described. Endogenous Gα12 was localized in normal mouse glomeruli by immunohistochemistry. At lower power, Gα12 was detected in the glomeruli (Figure 1a, arrow heads) and throughout the nephron. At higher power (Figure 1a, black/white arrows), several cell types, including podocytes, appear to express Gα12 with only background staining in the controls (antibody preincubated with peptide) (Figure 1a, b and d). To confirm podocyte Gα12 localization, immunogold EM was performed (Figure 1b). Gold particles were visible in several locations, including FPs, major processes (MPs) and at the branch points proximal to the FPs (arrows). The localization of Gα12 in MPs suggests that Gα12 may have functions not directly related to slit diaphragm permeability.
Establishing Transgenic Mice with Podocyte Expression of Activated Gα12 (QLα12)
Mice expressing EE-tagged, human Gα12 (Q229L) were established using a LacZ/floxed transgenic approach24 (Figure 2a). For conditional expression,28 QLα12LacZ+/Cre− mice were crossed with Nphs2-Cre mice (Nphs2 is efficiently expressed in podocytes and no other glomerular cells).29 Cre-mediated excision of the LacZ/stop in podocytes resulted in QLα12 expression (QLα12LacZ+/Cre+) (Figure 2). The CMV promoter is associated with the mosaic expression due to random inactivation,24 although Cre efficiently excises LacZ in these podocytes. QLα12LacZ+/Cre− (Control) and QLα12LacZ+/Cre+ mice at 2 months of age (littermates) were stained for β-gal using standard techniques. β-Gal staining revealed that transgene expression was low in proximal tubules, but higher in the papilla (as reported,24 not shown). QLα12LacZ+/Cre+ mice showed a major reduction in glomerular LacZ staining (Figure 2b) compared with Cre− controls. A semiquantitative analysis of β-gal staining suggested that random inactivation of the CMV promoter in podocytes led to LacZ expression in approximately half of the targeted cells (Supplementary Figure 1). Note that all the Cre+ mice showed significantly less or no β-gal staining, while the controls were evenly distributed (Supplementary Figure 1). The small amount of residual LacZ staining in QLα12LacZ+/Cre+ mice likely reflects LacZ expression in mesangial or endothelial cells. To distinguish EE-QLα12 expression from endogenous Gα12, control and QLα12LacZ+/Cre+ kidneys were co-stained with anti-nephrin and anti-EE antibodies (Figure 2c). In control mice, nephrin was seen throughout the glomerulus, with no detectable EE staining (Figure 2c and a). In QLα12LacZ+/Cre+ mice, both proteins were detected (Figure 2c and b), and as expected from immunoEM (Figure 1b), there was little overlap with nephrin in the slit diaphragm. ImmunoEM using the EE epitope antibodies confirmed transgenic QLα12 expression (not shown) in a similar distribution to the endogenous Gα12 (Figure 1b). To confirm that active Gα12 was expressed in these glomeruli, GST-TPR pull downs were performed as described previously;16 the TPR domain of PP5 binds the active conformation of Gα12/13.30 Figure 2d shows GST-TPR pull downs of thrombin-stimulated MDCK cells compared with cortical kidney lysates from QLα12LacZ+/Cre+ and QLα12LacZ+/Cre− mice.
QLα12 Expressed in Podocytes Leads to Age-Dependent Proteinuria
Development of microalbuminuria is a sensitive marker for podocyte injury.31 Urine was analyzed for albumin/creatinine ratio (Figure 3a) from QLα12LacZ+/Cre+ and control mice every 2 months. Microalbuminuria (albumin/creatinine ratios ≥34) appeared in a few control mice at 4–6 months, but was seen in ∼40% of QLα12LacZ+/Cre+ mice (some with ratio >200; Figure 3 and Table 1). Most of the QLα12LacZ+/Cre+ mice developed proteinuria as they aged, whereas only a few controls had mildly increased levels. Coomassie blue staining of urine confirmed the expression of albumin (Figure 3b). The magnitude of proteinuria continued to increase until mice were sacrificed at 22–26 months. On the basis of this phenotype, we divided the mice into young (<6 months; occasional mild proteinuria) and older (>12 months; frequent and often severe proteinuria) for further analysis.
The lack of proteinuria in most younger mice suggests that podocytes compensate for the expression of activated Gα12. To test whether QLα12 expression predisposed younger mice (<6 months) to injury, proteinuria was examined after LPS stimulation (a model of transient podocyte injury) (Figure 3c).32 Baseline proteinuria was similar, and 18 h after LPS stimulation, control mice increased proteinuria 1.9-fold, whereas QLα12LacZ+/Cre+ mice showed a significantly larger increase (3.6-fold). However, these short-term experiments did not detect any differences in ultrastructural or light microscopy findings (not shown). These findings are consistent with LPS-stimulated podocyte actin cytoskeleton changes leading to proteinuria and reveal that podocyte expression of QLα12 in young mice enhances the injury response.
Focal GS Develops with Age in QLα12LacZ+/Cre+ Mice
In mice aged <6 months, no significant renal histopathological changes were observed in control or QLα12LacZ+/Cre+ mice (Figure 4a and e). However, by 12–18 months, QLα12LacZ+/Cre+ mice showed numerous globally and segmentally sclerosed glomeruli, whereas controls exhibited only rare sclerosed glomeruli. Quantifying affected glomeruli showed a >6-fold increase in sclerosed glomeruli from kidneys of QLα12LacZ+/Cre+ mice (n=7) compared with controls (n=7) (Figure 4b and f). Segmentally sclerosed glomeruli were characterized by hyalinosis, GBM reduplication, isolated epithelial cells containing PAS+ protein reabsorption granules and adhesion of the tuft to Bowman's capsule. Mesangial areas of QLα12LacZ+/Cre+ mice exhibited mild to focally moderate expansion by matrix and cells, whereas the mesangial areas of control mice exhibited minimal expansion (Figure 4b and f, arrow). The kidneys of 18-month-old mice exhibited changes similar to the 12- to 18-month group, but more prominent. On average, 4.79% of glomeruli in QLα12LacZ+/Cre+ mice were globally or segmentally sclerosed, whereas only 0.16% of glomeruli in control mice were sclerosed (Figure 4c–e and h). Over 73% of these sclerosed glomeruli were juxtamedullary. Focal segmental double contours (areas of GBM redundancy) were more commonly seen in QLα12LacZ+/Cre+ mice than in controls. Occasional PAS+ hyaline casts were observed in the medulla to varying degrees in older animals as well as focal interstitial mononuclear inflammation, usually in association with focal GS (Figure 4g and h, arrows). Features of focal tubular injury, including tubular luminal distension, epithelial flattening and microcyst formation, were seen in a subset of the oldest QLα12LacZ+/Cre+ mice, and these findings were not seen in the age-matched controls. The non-lesional glomeruli (Figure 4h, arrowhead) exhibit moderate mesangial expansion by matrix and cells (*). Other features of active or chronic tubulointerstitial or vascular pathology were not observed in any animals. There were no detectable differences in serum creatinine between the 14- and 19-month-old QLα12LacZ+/Cre+ mice and controls as all values were ≤0.2 mg/dl.
Older Qlα12LacZ+/Cre+ Mice Develop GBM Irregularities, Mesangial Expansion and FP Fusion
EM of QLα12LacZ+/Cre+ and control mice <6, 12–18 and >18 months were scored for ultrastructural abnormalities. The youngest QLα12LacZ+/Cre+ mice (Figure 5a) appeared normal. At 12–18 months, there was little difference in the GBM or the podocytes, but most QLα12LacZ+/Cre+ mice had focal features of endothelial injury, including double contours, subendothelial granular electron-dense deposits and cell swelling (Figure 5e, arrowhead; not seen in the controls). In addition, QLα12LacZ+/Cre+ scored higher for mesangial expansion by matrix and cells (Figure 5e (*); Table 2). Older QLα12LacZ+/Cre+ mice (>18 months) (Figure 5f) mice exhibited a greater degree of FP effacement and irregularity, presumably associated with aging (Figure 5f, arrows) than was seen in the controls (although controls did reveal some age-related changes). There were few differences in the GBM, but there were an increased number of subepithelial GBM membrane projections in the oldest transgenics (Figure 5f, ♦). All mice exhibited mild mesangial expansion at >18 months; however, QLα12LacZ+/Cre+ mice showed more severe signs of mesangial abnormalities (Figure 5f, *), and overall scores (Table 2) for glomerular injury were twice as high in the QLα12LacZ+/Cre+ mice (Figure 5c).
QLα12LacZ+/Cre+ Mice have Normal Numbers of Podocytes
On the basis of the morphological changes, we examined podocyte number by WT-1 staining.33 Surprisingly, podocyte number/glomerulus showed no difference between QLα12LacZ+/Cre+ and controls >12 months (Figure 6a and b). As Gα12 can stimulate both proliferation and apoptosis,16 WT-1-positive cells were quantified for apoptosis (TUNEL) and proliferation (Ki67) to exclude the possibility that podocyte number was preserved through QLα12 co-stimulation of proliferation and apoptosis. No apoptotic podocytes were seen in QLα12LacZ+/Cre+ or controls (Figure 6a, middle panels), nor any difference observed in proliferation (not shown). In addition, there were no differences in the number of glomeruli in kidneys obtained from QLα12LacZ+/Cre+ mice as compared with controls (data not shown). This indicates that the development of proteinuria and GS does not result from developmental effects on glomeruli number or podocyte depletion, and other mechanisms must be responsible.
QLα12 Expression in Podocytes does not Lead to Rho or Src Activation
Next, Rho activity was examined in QLα12Lac+/Cre+ and control mice. Rho activity was determined by ELISA on glomerular isolates from young and old mice. There were no significant differences in Rho activity from QLα12Lac+/Cre+ mice compared with controls, nor was there any difference in Rho protein expression (Figure 7a). Gα12 also activates Src,34 and western blot using pY419 antibodies failed to demonstrate any differences in Src activation (not shown). This finding suggests that podocytes compensate for persistent QLα12 expression and employs other mechanisms to prevent sustained Rho or Src activation.
Real-Time PCR Showed no Changes in Podocyte-Specific Genes, but QLα12LacZ+/Cre+ Mice Exhibit Dysregulated Glomerular Collagen Expression
Real-time PCR of glomerular isolates failed to detect changes in expression levels of several major podocyte genes implicated in FSGS (Nphs1, Nphs2, CD2AP and TRPC6) (Supplementary Figure 2). The normal adult GBM is composed predominantly of α3, α4 and α5(IV) collagen and laminin-11 (α5β2γ1).35 During development, the normal GBM is composed of α1 and α2(IV) collagen that is followed by a switch to α3, α4 and α5(IV) expression in mature glomeruli. Real-time PCR of Col4a showed increased Col4a2 transcript expression levels in younger mice (Figure 8a), although the variability in phenotype led to wide confidence intervals. These age-dependent changes are likely to contribute to the observed variability, and as this analysis was performed on isolated glomeruli, it is possible that the most severely sclerotic glomeruli were not included and would thus tend to underestimate the differences. In addition, the real-time results were reanalyzed in male and female mice, and no differences were observed to account for the differences in phenotype seen between transgenic and control mice in any age group (not shown). Nevertheless, when considered together, these results suggest a change in the relative balance of specific collagen α(IV) chains, and immunofluorescence microscopy confirmed mildly increased collagen α1/2(IV) expression (Figure 8c). In older mice, Col4a2 expression abnormalities persist, and by 12–14 months, there is also a twofold increase in Col4a1 and decreased Col4a5 (Figure 8c). Consistent with the real-time results, collagen α1/2(IV) staining was increased in older mice, and the localization was disorganized without clear capillary loop staining (Figure 8d). Decreased expression of α5 was confirmed, although the pattern of staining appeared similar to the control (Figure 8d). No other α(IV) chains showed differences in staining. The α3/4/5 antibody showed decreased intensity compared with the control, and based on the α3 staining and real-time results, this difference is most likely explained by the decreased α5 collagen expression. To determine if QLα12 directly regulates Col4 gene expression, a previously characterized inducible (tet off) QLα12-MDCK cell line10, 11, 17 was analyzed by microarray. Supplementary Figure 3 shows 7- and 10-fold increase in Col4a1 and Col4a2, respectively, within 72 h of QLα12 expression. Taken together, this analysis indicates that QLα12 expression leads to dysregulated Col4a gene expression before the onset of proteinuria and suggests a mechanism where activated Gα12 alters Col4 gene expression.
DISCUSSION
Understanding the mechanisms of CKD progression is important for finding new therapeutic targets. Podocytes are exposed to approximately 180 l of ultrafiltrate per day that contains biologically active molecules, including hormones, cytokines and filtered proteins. Herein, we demonstrate that activation of Gα12 in podocytes leads to age-dependent proteinuria and focal GS through a distinct mechanism involving dysregulated collagen α(IV) expression without podocyte depletion. This supports the hypothesis that filtered agonists activate podocyte signaling pathways and can contribute to progressive glomerular injury. Although Gα12 regulates numerous processes that could lead to podocyte damage, including apoptosis, proliferation, cell attachment, actin cytoskeletal changes and junctional regulation, these do not explain the time-dependent phenotype in these mice. Rather, a novel mechanism of collagen α(IV) abnormalities appears to be responsible.
Testing the notion that podocytes respond to filtered molecules is difficult to examine in vivo. Expressing a constitutively active G protein allows for the identification of downstream effector pathways, and this approach led to the identification of h-Ras as an oncogene.36 This strategy could identify novel therapeutic targets to prevent GS without the need to identify the specific GPCR or its ligand. Analogous to our studies, activated (QL) Gαq was targeted to podocytes,37 and these mice developed proteinuria through a different mechanism. QLαq mice had developmental defects, including smaller kidneys and reduced nephron number. There was downregulation of nephrin and other podocyte genes, although Col4a was not examined. QLαq mice were more susceptible to puromycin aminonucleoside injury, and a subset of mice developed GS at 6 months of age, a finding that could occur from reduced nephron number. In contrast, QLα12 in podocytes did not affect renal development, nephron or podocyte number, and revealed altered regulation of collagen and GBM abnormalities.
Proteinuria developed in some QLα12LacZ+/Cre+ mice by 4–6 months before morphological changes (Figures 3, 4 and 5). By 12–14 months, there was some FP fusion that could account for the increase in proteinuria; however, the CMV promoter results in mosaic expression, and we estimate that only about half of podocytes express QLα12 (Figure 2). This focal expression is likely to account for the mild phenotype, but also recapitulates local differences in glomerular response to stress and the focal nature of GS. In fact, the majority of sclerotic glomeruli was located at the cortico-medullary junction, a region where the glomeruli are more susceptible to hemodynamic stress.38, 39 Why some younger mice manifest proteinuria before morphological changes are detected is not clear. They do not show enhanced baseline Rho or Src activity, but they are more susceptible to LPS-induced proteinuria (without changes in Col4 gene expression (not shown)), suggesting that QLα12 can promote functional effects on permeability. The absence of ultrastructural changes in young mice or after LPS suggests that perhaps through Gα12 localization in the major FPs, it regulates cortical actin and permeability. Although only about 5% of the glomeruli develop GS, proteinuria is likely to arise from a more global effect on significantly more glomeruli. This is based on the observation that QLα12 is expressed in approximately 50% of glomeruli (Supplementary Figure 1) and QLα12 expression increased proteinuria in LPS-treated mice without morphological or ultrastructural changes.
Mesangial expansion was prominent in the QLα12LacZ+/Cre+ mice, and mesangial expansion is particularly characteristic of FSGS, membranous and diabetic nephropathy, Alport's syndrome and Denys–Drash syndrome.40 Mesangial expansion can contribute to proteinuria through a mechanism that does not require podocyte depletion (as seen in this study).41 In addition, both nephropathy and Alport's syndrome are both characterized by changes in collagen expression.42, 43, 44 Transcription of Col4a1 and A2 is co-regulated by the same promoter owing to head-to-head orientation in chromosome 13q34.45 As a result, diseases affecting collagen IV expression will be associated with changes in both α1(IV) and α2(IV)) as seen in both the above disease and QLα12 mice (Figure 8). However, Alport's syndrome is caused by mutations in α3, α4 or α5(IV) collagen, leading to sustained α1 and α2(IV) expression, whereas membranous nephropathy results from autoantibodies46 that promote podocyte injury and subepithelial deposits.
On the basis of the time course of proteinuria and morphological changes, we speculate that Gα12 modifies COL4 gene expression and the resulting basement membrane and endothelial abnormalities accrue with time. However, whether these changes reflect age-dependent effects or are the result of cumulative Gα12 activation will require additional study. Since C57/B6 mice develop mild ultrastructural changes, cytokine activation and proteinuria with age (as seen in some control mice), the phenotypic differences seen in the older mice with Gα12 activation could represent indirect effects on cumulative age-related changes in this mouse strain, or a cumulative time effect of Gα12 activation. Although the control mice do develop mild changes with age, the dramatic differences seen in the age- and sex-matched QLα12 mice strongly implicates the persistent Gα12 activation as the etiology. Furthermore, the finding of upregulated COL4A1/2 seen with QLα12 expression in MDCK cells (Supplementary Figure 3) supports an important link between Gα12 activation and Col4A1/2 gene expression.
Although Gα12 stimulates TGFβ20, 21 and could promote GS, we were unable to detect increased TGFβ in either young or old mice (not shown). However, an alternative mechanism is suggested by the increased number of subepithelial GBM membrane projections (Figure 5) similar to findings seen in DDR1 and integrin α2 knockout mice.47, 48 DDR1 is a tyrosine kinase receptor for collagen IV, and we previously showed that Gα12 regulates α2β1 integrin signaling and attachment,17 thus suggesting that persistent Gα12 activation might lead to podocyte changes in integrin signaling. The onset of proteinuria is during adulthood and virtually all mice have proteinuria by mid-life (12–16 months, normal C57/B6 mouse lifespan ∼3 years49). With aging, podocytes may accumulate damage from a variety of sources, including GPCR-linked inflammatory and vasoactive mediators. ROS, inflammation and vascular changes occur in hypertensive and diabetic kidney disease, and ROS directly activates Gα subunits.50 On the basis of these observations, we suggest that this model of slowly progressive kidney disease will be valuable for understanding CKD progression in humans. Consistent with GPCR pathways regulating age-related kidney damage, AT1 receptor knockout mice exhibit decreased oxidative damage and outlive their wild-type littermates.51 The glomerular filtration rate declines with age, but it is unclear whether this is part of normal aging or represents injury from cumulative exposures. If decreased renal function and GS occur through repetitive activation of podocyte signaling pathways, it is tempting to speculate that inhibiting Gα12 pathways in podocytes may provide new treatments to protect renal function and delay CKD progression.
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Acknowledgements
We thank Dr Dorin-Bogdan Borza for his consultation and the gift of the collagen antibodies; Drs Johannes Schlondorff and Michael Ross for their knowledge of podocytes and glomerular isolation; and Andrea Bernhardy for her technical assistance. In addition, we thank Dr Dennis Brown and Margaret McLaughlin for help with the immunoelectron microscopy, and Dr Hui Chen and Colleen Ford for their technical assistance with EM of the transgenic mice. This research was supported by NIH Grants R21DK065932 and R01GM055223 to BMD. IB was supported by NIH training Grant T32DK007527. IB conceived and carried out experiments, analyzed data, prepared figures and contributed to writing and editing the paper; WY, SB, HN and MT conceived and carried out experiments and analyzed data; MP contributed to editing the paper; JH carried out experiments, analyzed data, prepared figures and contributed to editing the paper; and BMD conceived of experiment and contributed to writing and editing the paper.
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Supplementary Information accompanies the paper on the Laboratory Investigation website (http://www.laboratoryinvestigation.org)
Constitutive activation of the G protein Gα12 in podocytes promotes proteinuria and glomerulosclerosis through a novel mechanism involving changes in collagen (α)IV expression without podocyte depletion. Thus, persistent G-protein-coupled receptor activation can lead to glomerular damage.
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Boucher, I., Yu, W., Beaudry, S. et al. Gα12 activation in podocytes leads to cumulative changes in glomerular collagen expression, proteinuria and glomerulosclerosis. Lab Invest 92, 662–675 (2012). https://doi.org/10.1038/labinvest.2011.198
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DOI: https://doi.org/10.1038/labinvest.2011.198
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