Dear Editor,
Promoting the functional maturation of the desired cell types derived from human pluripotent stem cells (hPSCs) remains a major challenge, especially for hepatocytes, as routine access to metabolically functional hepatocytes would enable their use in drug toxicity screening. Although previous attempts to induce hepatic specification from hPSCs yielded cells possessing some hepatic features1,2,3, most of these cells showed poor metabolic activities, and the responsible mechanisms are unknown. In this study, we explored the intrinsic defects in hPSC-derived immature hepatocytes and tested whether correcting these defects would promote the metabolic maturation of the differentiated cells.
One possible explanation for the inability to derive mature hepatic cells is incomplete specification of the differentiated cells4 caused by the lack of some key transcription factors. To test this hypothesis, immunofluorescence staining was carried out to assess the co-expression of a panel of crucial transcription factors, including FOXA2, GATA4, HNF4A, GATA6, PROX1, HNF6 and TBX3, along with the mature hepatocyte markers ALB and CYP3A4 in hepatocyte-like cells differentiated from human embryonic stem cells (hESCs), according to a previously published protocol5. Although ALB was efficiently expressed and co-localized with HNF4A in hepatocyte-like cells differentiated from hESCs, CYP3A4 was rarely observed in the differentiated cultures (Supplementary information, Data S1 and Figure S1A–S1B). Surprisingly, the limited expression of CYP3A4 correlated well with the expression of PROX1 and HNF6, which also were rarely observed in the cultures. When cells at the hepatoblast stage were characterized, similar defects were already present (Supplementary information, Figure S1C–S1E). Although FOXA2, GATA4, HNF4A and GATA6 were expressed ubiquitously (> 95%) in hESC-derived AFP-positive cells, PROX1 and HNF6 were barely detectable (< 0.1%). Interestingly, in mouse embryos, a deficiency in PROX1 or HNF6 in hepatoblasts does not disturb the emergence of Alb+ cells from the endoderm6,7. Therefore, these results suggest that two populations can be distinguished during hepatic differentiation from hESCs based on the expression of PROX1 and HNF6. The cells lacking PROX1 and HNF6 expression at an early hepatoblast stage, although retaining ALB expression, are incompletely programmed and fail to complete late metabolic maturation.
Given the importance of PROX1 and HNF6 in liver organogenesis together with the fact that these proteins were co-expressed with CYP3A4, we tested whether the robust induction of PROX1 and HNF6 expression during hepatic differentiation would promote efficient hepatic metabolic maturation. After the induction of hESCs through definitive endoderm cells8 to ventral foregut cells (Figure 1A and Supplementary information, Figures S2 and S3), the cells are recruited to the hepatic lineage. Because both BMP and FGF signals were included in our previous protocol5, we postulated that an additional signal is required for hepatic PROX1 and HNF6 expression. Among the various conditions that we tested, we found that replating dissociated ventral foregut cells at a relatively low density (2 × 105/ml) followed by treatment with induction factors (FGF7, BMP2 and BMP4) for 5 days greatly promoted the emergence of PROX1- and HNF6-expressing hepatoblast-like colonies; these colonies were also positive for AFP, HNF4A and TBX3 (Supplementary information, Figure S4). Intracellular flow cytometric analysis revealed an overall 63.7% efficiency for the correct induction of hepatoblasts, as characterized by the co-expression of HNF4A and PROX1 (Figure 1D). Quantitative PCR analysis confirmed that PROX1 and HNF6 expression could be efficiently induced only when both the induction factors were present and the replating procedure was performed (Figure 1B). In contrast, the expression of AFP, HNF4A and ALB showed no such change under the replating treatment (Supplementary information, Figure S4C). After exposure to HGF and BMP4 to promote further hepatic commitment, the hepatoblast-like colonies expanded extensively and started expressing HNF1A and CEBPA, which are expressed at later stages of liver development, while retaining the expression of HNF4A, PROX1 and HNF6 (Figure 1C). Interestingly, the hESC-derived hepatoblasts showed differentiation plasticity (Supplementary information, Figure S5) similar to their in vivo counterparts.
To determine whether the hepatoblasts would efficiently mature into hepatocytes that express metabolic enzymes now that the specification defects had been corrected, cultures were treated using the further maturation conditions that have been previously reported5. At the end of the differentiation period, CYP3A4 was expressed in extensive regions of the hepatic clusters but was confined to PROX1- and HNF6-expressing cells (Figure 1E and Supplementary information, Figures S7A and S10), consistent with our initial findings. Based on flow cytometric analysis, nearly 70% of the cells were double-positive for the hepatic surface marker ASGPR and the fluorescent metabolite of the CYP3A4-specific substrate DBOMF (Figure 1D), which was verified by immunofluorescence in the absence or presence of the CYP3A4-specific inhibitor ketoconazole (Supplementary information, Figure S7D). In addition, the hESC-derived hepatocytes (hEHs) co-expressed the hepatic functional gene ALB along with CYP3A4, CYP1A2, CYP reductase and AAT (Figure 1E and Supplementary information, Figure S7A–S7B). Moreover, these cells co-expressed the transcription factors PROX1, HNF6, HNF1A, CEBPA, HNF4A, FOXA2, HNF1B and CEBPB, all of which are essential for liver morphogenesis9, whereas TBX3 and GATA4 were downregulated (Supplementary information, Figures S2 and S6), as occurs in vivo during the advancement of hepatocyte differentiation. For further functional validation, hEHs were assessed using a panel of functional assays. The secretion of albumin and α1-antitrypsin increased greatly to levels comparable to those of primary human hepatocytes (Figure 1F) and much higher than those in previous reports2,5. Moreover, Oil red O staining demonstrated the presence of lipid droplets in the CYP3A4-expressing hEHs (Figure 1E); periodic acid-Schiff staining showed glycogen synthesis; and incubation with indocyanine green demonstrated the uptake ability of the hEHs. The capacity to engraft into the liver parenchyma in vivo was also verified by immunostaining and by the secretion of human albumin into the plasma (Supplementary information, Figure S7E–S7G). When this newly developed protocol (Figure 1A) was applied to the induced pluripotent stem cells (3U1 line)1, similar results were obtained (Supplementary information, Figure S7C).
To confirm the requirement for PROX1 and HNF6 in regulating the formation of CYP-expressing hepatocytes from hESCs, we attempted to knockdown the expression of PROX1 and HNF6 by lentivirus-mediated shRNA at the hepatoblast stage of the newly developed protocol (Supplementary information, Figure S8). Compared with the control shRNA-treated cells, the PROX1 or HNF6 shRNA-treated cells failed to differentiate into CYP3A4-expressing cells, as indicated by a marked decrease in immunofluorescence-positive cells and by a great reduction in albumin secretion and CYP3A4 metabolic activity. Quantitative PCR analysis showed that the knockdown of either PROX1 or HNF6 impaired the expression of HNF1A in addition to ALB and CYP3A4, and had little or no effect on the expression of FOXA2 and HNF4A, suggesting that the regulation of hepatic differentiation by PROX1/HNF6 may not rely on HNF4A/FOXA2.
To evaluate the suitability of the newly generated hEHs for drug metabolism studies, we systematically characterized the phase I CYP and phase II conjugation activities using the only determination method approved by the FDA (standard chemical substrates by LC/MS/MS analysis). We found that the top five major CYP enzymes, which are responsible for ∼85% of known oxidative drug metabolism, showed activity in the hEHs, and the most important phase II glucuronidation enzymes were also active (Figure 1G). Among these enzymes, the expression of CYP1A2 and 2C19 can only be detected well after birth in the human liver10. The activities of CYP3A4, 2C9, 2E1 and UGT in the hEHs were comparable to or higher than the activities in 25-week-old fetal hepatocytes. These data suggest that hEHs are differentiated to a state that possesses hepatic metabolic activity that is most likely to be similar to that of hepatocytes at the perinatal stage. Moreover, we observed the phase III transport of carboxy-dichloro-fluorescein diacetate (CDF) and cholyl-lysyl-fluorescein (CLF) into bile canaliculi between the hEHs. Immunostaining also showed that MRP2, which mediates CDF efflux, was co-expressed with CYP3A4 and had a similar pattern of localization in the canalicular space (Figure 1H).
To further determine the utility of hEHs in drug development, we assessed the potency of CYP activity modulation in mediating drug interactions and the ability of the hEHs to predict drug toxicity. When hEHs were incubated with the AhR activator omeprazole, the metabolic activity of CYP1A2 increased by over 5-fold, indicating that hEHs are susceptible to drug induction (Figure 1I). After treatment with ketoconazole and furafylline, model inhibitors of CYP3A4 and CYP1A2, respectively, the hEHs exhibited a dose-dependent reduction in metabolite production (Figure 1J). To evaluate drug-induced hepatotoxicity in the hEHs, we assessed the toxicity of troglitazone, which was withdrawn by the FDA due to hepatotoxicity, and its structural analog rosiglitazone (Supplementary information, Figure S7H). After the hEHs were exposed to the drugs for 24 h, the reduction in the mitochondrial activity of the treated hEHs was quantified to measure the hepatotoxicity of these compounds (Figure 1K). Troglitazone exhibited dose-dependent toxicity, with a TC50 value of approximately 0.25 mM. In contrast, rosiglitazone exhibited far less toxicity in the hEHs, and both compounds had little toxic effect on hESCs.
In this study, we efficiently generated a relatively homogenous population of hepatocytes from hPSCs. These hepatocytes expressed the metabolic enzymes responsible for ∼85% of known oxidative drug metabolism activities. Utilizing model compounds and common pharmacological assays, we demonstrated that these hEHs can be successfully used to measure metabolic activities, to conduct preliminary screening for the inhibitory effects of new drugs, and to evaluate drug-induced cytotoxicity. In addition, we discovered that the acquisition of metabolic activity by hEHs depends on the activation of PROX1 and HNF6, which are regulated in parallel to HNF4A, FOXA2 and other factors (Supplementary information, Figure S9). Taken together, our findings provide an important insight into the transcriptional regulation of hepatic specification and maturation and represent a critical step toward the generation of hepatocytes for use in drug discovery.
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Acknowledgements
We thank Iain C Bruce for critical reading of the manuscript, Yizhe Zhang and Liying Du for technical support on real-time PCR and FACS analysis, respectively. This work was supported by grants from the National Basic Research Program of China (973 program; 2012CD966401 and 2009CB522501), Bill & Melinda Gates Foundation Grant (1023963 and 37871), the Ministry of Education of China (111 project), Beijing Science and Technology Plan (Z121100005212001) and National Science and Technology Major Project (2012ZX10004-503) to HD.
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( Supplementary information is linked to the online version of the paper on the Cell Research website.)
Supplementary information
Supplementary information, Data S1
Materials and Methods (PDF 68 kb)
Supplementary information, Figure S1
Immunofluorescence analysis of expression of transcriptional factors in hepatic differentiation using the previous protocol. (PDF 389 kb)
Supplementary information, Figure S2
Dynamic expression of key markers of hepatic differentiation. (PDF 112 kb)
Supplementary information, Figure S3
Fluorescence micrographs showing marker expression at the definitive endoderm and ventral foregut stages. (PDF 537 kb)
Supplementary information, Figure S4
Generation of hepatoblasts expressing PROX1 and HNF6. (PDF 174 kb)
Supplementary information, Figure S5
Transdetermination from a hepatic fate to a pancreatic or pulmonary fate. (PDF 317 kb)
Supplementary information, Figure S6
Immunofluorescence analysis of expression of liver transcription factors in hEHs. (PDF 164 kb)
Supplementary information, Figure S7
Characterization of hEHs. (PDF 244 kb)
Supplementary information, Figure S8
shRNA-mediated inhibitions of PROX1 and HNF6 expression in the differentiation process perturbs hepatic specification. (PDF 371 kb)
Supplementary information, Figure S9
Schematic model of regulation of hepatic maturation by multiple transcription factors and different signals. (PDF 69 kb)
Supplementary information, Figure S10
IgG controls for immunofluorescence staining. (PDF 187 kb)
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Zhao, D., Chen, S., Duo, S. et al. Promotion of the efficient metabolic maturation of human pluripotent stem cell-derived hepatocytes by correcting specification defects. Cell Res 23, 157–161 (2013). https://doi.org/10.1038/cr.2012.144
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DOI: https://doi.org/10.1038/cr.2012.144
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