Featured
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Letter |
Differential L1 regulation in pluripotent stem cells of humans and apes
Induced pluripotent stem-cell characterization reveals phenotypical differences between humans and non-human primates (NHPs): gene expression analysis shows differences in the regulation of long interspersed element-1 (L1) transposons, and in the expression of L1-restricting genes APOBEC3B and PIWIL2, correlating with higher L1 mobility in NHPs; this indicates that L1 mobility differences may have differentially shaped the human and NHP genomes.
- Maria C. N. Marchetto
- , Iñigo Narvaiza
- & Fred H. Gage
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Article |
Deterministic direct reprogramming of somatic cells to pluripotency
This study shows that the combination of naive pluripotency growth conditions, Oct4, Sox2, Klf4 and Myc (OSKM) overexpression, and depleting the Mbd3/NuRD co-repressor results in deterministic and synchronized reprogramming to pluripotency.
- Yoach Rais
- , Asaf Zviran
- & Jacob H. Hanna
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Letter |
The pluripotent genome in three dimensions is shaped around pluripotency factors
Using 4C technology, higher-order topological features of the pluripotent genome are identified; in pluripotent stem cells, Nanog clusters specifically with other pluripotency genes and this clustering is centred around Nanog-binding sites, suggesting that Nanog helps to shape the three-dimensional structure of the pluripotent genome and thereby contributes to the robustness of the pluripotent state.
- Elzo de Wit
- , Britta A. M. Bouwman
- & Wouter de Laat
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Letter |
Vascularized and functional human liver from an iPSC-derived organ bud transplant
Vascularized, functional human liver is generated from human induced pluripotent stem cells (iPSCs) by transplantation of liver buds created in vitro (iPSC-LBs); hepatic cells self-organized into three-dimensional iPSC-LBs, and human vasculatures in iPSC-LB transplants became functional by connecting to host vessels, stimulating maturation of iPSC-LBs into tissue resembling adult liver and performing liver-specific functions.
- Takanori Takebe
- , Keisuke Sekine
- & Hideki Taniguchi
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Letter |
AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes
Fibroblasts deficient in the activation-induced cytidine deaminase (AID) enzyme are shown to fail to stabilize in the pluripotent state, despite initiating the expression of pluripotency genes.
- Ritu Kumar
- , Lauren DiMenna
- & Todd Evans
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Letter |
NANOG-dependent function of TET1 and TET2 in establishment of pluripotency
The authors show that the pluripotency factor NANOG interacts with TET1 and TET2 methylcytosine hydroxylases, and that the hydroxylation of 5-methylcytosine to 5-hydroxymethylcytosine enhances the efficiency of somatic cell reprogramming in a NANOG-dependent manner.
- Yael Costa
- , Junjun Ding
- & Jianlong Wang
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Letter |
Studying arrhythmogenic right ventricular dysplasia with patient-specific iPSCs
This study demonstrates that an inheritable adult onset heart disease can be modelled in vitro within months with the help of metabolic maturation induction.
- Changsung Kim
- , Johnson Wong
- & Huei-Sheng Vincent Chen
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Letter |
Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells
Immune rejection may limit the therapeutic use of induced pluripotent stem cells (iPSCs); here, terminally differentiated mouse iPSCs are shown to generate negligible immune rejection in their host.
- Ryoko Araki
- , Masahiro Uda
- & Masumi Abe
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News |
Brain cells made from urine
Human excreta could be a powerful source of cells to study disease, bypassing some of the problems of using stem cells.
- Monya Baker
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Letter |
Somatic copy number mosaicism in human skin revealed by induced pluripotent stem cells
A whole-genome and transcriptome analysis of 20 human induced pluripotent stem-cell lines shows that reprogramming does not necessarily add de novo copy number variants to what is already present in the somatic cells from which they originated.
- Alexej Abyzov
- , Jessica Mariani
- & Flora M. Vaccarino
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News |
Stem-cell transplant claims debunked
Transplant of induced pluripotent stem cells to treat heart failure probably never happened.
- David Cyranoski
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Letter |
Early-stage epigenetic modification during somatic cell reprogramming by Parp1 and Tet2
Parp1 and Tet2 mediate essential epigenetic remodelling events in an early phase of induced pluripotent stem cell reprogramming, thus directing subsequent induction of the pluripotency loci.
- Claudia A. Doege
- , Keiichi Inoue
- & Asa Abeliovich
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News |
Stem-cell pioneer banks on future therapies
Japanese researcher plans cache of induced stem cells to supply clinical trials.
- David Cyranoski
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News |
Rudimentary liver grown in vitro
Japanese scientists coax pluripotent cells to form functional liver tissue.
- David Cyranoski
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News |
Alzheimer's 'in a dish' shows promise
Reprogrammed cells suggest path towards early diagnosis and drug development.
- Ewen Callaway
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Letter |
Probing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells
Induced pluripotent stem cells are shown to be useful for studying phenotypes relevant to familial and sporadic Alzheimer’s disease, even though it can take decades for the disease to manifest in patients.
- Mason A. Israel
- , Shauna H. Yuan
- & Lawrence S. B. Goldstein
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Review Article |
The promise of induced pluripotent stem cells in research and therapy
- Daisy A. Robinton
- & George Q. Daley
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Article |
DNA-binding factors shape the mouse methylome at distal regulatory regions
Base-pair-resolution genomic maps of DNA methylation are generated in the mouse, providing new insights in gene regulation.
- Michael B. Stadler
- , Rabih Murr
- & Dirk Schübeler
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News & Views |
Organ recital in a dish
It is difficult to recapitulate organ development in vitro, especially when interactions between tissues are essential. Nonetheless, researchers have now achieved this for the pituitary gland. See Article p.57
- Karine Rizzoti
- & Robin Lovell-Badge
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Letter |
Excitation-induced ataxin-3 aggregation in neurons from patients with Machado–Joseph disease
Human neurons derived from induced pluripotent stem cells permit the study of aberrant protein processing and aggregation in Machado–Joseph disease.
- Philipp Koch
- , Peter Breuer
- & Oliver Brüstle
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News |
Host neurons obey transplants
Neurons derived from human embryonic stem cells can control native neurons in mice.
- Charlotte Schubert
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Letter |
Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease
A new strategy for derivation of human midbrain dopamine neurons from pluripotent cells was developed; transplantation of the neurons in mice, rats and parkinsonian monkeys show they are a promising source of cells for applications in regenerative medicine.
- Sonja Kriks
- , Jae-Won Shim
- & Lorenz Studer
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News |
European court bans patents based on embryonic stem cells
Final decision could stifle investment in developing therapies.
- Ewen Callaway
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News |
Liver-disease mutation corrected in human stem cells
Patient-derived stem cells reprogrammed to become healthier.
- Susan Young
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Letter |
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
- Kosuke Yusa
- , S. Tamir Rashid
- & Ludovic Vallier
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Article |
Human oocytes reprogram somatic cells to a pluripotent state
- Scott Noggle
- , Ho-Lim Fung
- & Dieter Egli
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News & Views |
Bespoke cells for the human brain
Human skin cells have been directly converted into neurons, an achievement that could lead to the cell-based treatment of neurodegenerative disorders. But the road ahead remains long and tortuous. See Letters p.220, p.224 & p.228
- Michael Sendtner
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Research Highlights |
Edited stem cells yield healthy mice
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News & Views |
iPS cells under attack
Induced pluripotent stem cells offer promise for patient-specific regenerative therapy. But a study now cautions that, even when immunologically matched, these cells can be rejected after transplantation. See Letter p.212
- Effie Apostolou
- & Konrad Hochedlinger
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Letter |
Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1
- Momoko Maekawa
- , Kei Yamaguchi
- & Shinya Yamanaka
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Letter |
Induction of human neuronal cells by defined transcription factors
- Zhiping P. Pang
- , Nan Yang
- & Marius Wernig
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Letter |
Telomere shortening and loss of self-renewal in dyskeratosis congenita induced pluripotent stem cells
- Luis F. Z. Batista
- , Matthew F. Pech
- & Steven E. Artandi
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News Feature |
Stem cells: The growing pains of pluripotency
The field of induced pluripotent stem cells has grown up fast. Now it is entering the difficult stage.
- Erika Check Hayden
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News |
Reprogrammed cells trigger immune reactions in mice
Medical applications of induced pluripotent stem cells called into question.
- Erika Check Hayden
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News |
California ponders cell-banking venture
State agency grapples with technical and ethical challenges.
- Erika Check Hayden
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Research Highlights |
New ways to change fates
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Letter |
Modelling schizophrenia using human induced pluripotent stem cells
- Kristen J. Brennand
- , Anthony Simone
- & Fred H. Gage
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Letter |
Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells
- Hao Wu
- , Ana C. D’Alessio
- & Yi Zhang
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News & Views |
The dark side of induced pluripotency
Induced pluripotent stem cells have great therapeutic potential. But genomic and epigenomic analyses of these cells generated using current technology reveal abnormalities that may affect their safe use. See Articles p.58, p.63 & p.68
- Martin F. Pera
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Article |
Copy number variation and selection during reprogramming to pluripotency
Reprogramming of somatic cells to induced pluripotent stem (iPS) cells that can be differentiated into many cell types has great potential for personalized therapy. By comparing copy number variations of early- and intermediate-passage human iPS cells to their respective parental fibroblast cells and human embryonic stem (ES) cells, this study finds that a high mutation rate is associated with the reprogramming process. However, during moderate length culture, human iPS cells undergo a selection process leading to decreased mutation load of cells equivalent to that observed in human ES cells.
- Samer M. Hussein
- , Nizar N. Batada
- & Timo Otonkoski
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Letter |
Recapitulation of premature ageing with iPSCs from Hutchinson–Gilford progeria syndrome
- Guang-Hui Liu
- , Basam Z. Barkho
- & Juan Carlos Izpisua Belmonte
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News |
Flaw in induced-stem-cell model
Adult cells do not fully convert to embryonic-like state.
- Elie Dolgin
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Letter |
A unique chromatin signature uncovers early developmental enhancers in humans
Identifying the genomic regulatory sequences, such as enhancers, that control early embryonic development remains a difficult challenge. Here, profiling of histone modifications and chromatin regulators in human embryonic stem cells (hESCs) reveals unique signatures that are used to identify over 2,000 putative enhancers. These enhancers are either active in the h ESCs or associated with early developmental genes.
- Alvaro Rada-Iglesias
- , Ruchi Bajpai
- & Joanna Wysocka
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Letter |
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
Using a temporal series of growth factor manipulations to mimic embryonic intestinal development in culture, this study has successfully directed the differentiation of human pluripotent stem cells (both embryonic stem cells and induced pluripotent stem cells) into intestinal tissue. This approach may provide therapeutic benefit for disease studies.
- Jason R. Spence
- , Christopher N. Mayhew
- & James M. Wells
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Research Highlights |
Stem cells: Platelets get a boost
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News & Views |
Excessive mobility interrupted
Mobile DNA sequences called L1 contribute to the brain's genetic heterogeneity and may affect neuron function. The protein MeCP2, which is mutated in Rett syndrome, seems to regulate the activity of these genomic elements. See Letter p.443
- Lorenz Studer