Embryonic stem cells articles within Nature

Featured

  • Article
    | Open Access

    A genetically inducible stem cell-derived embryoid model of early post-implantation human embryogenesis captures the codevelopment of embryonic tissue and extra-embryonic endoderm and mesoderm niche with early haematopoiesis, with potential for drug testing and disease modelling.

    • Joshua Hislop
    • , Qi Song
    •  & Mo R. Ebrahimkhani

  • Article
    | Open Access

    Synthetic mouse embryos assembled from embryonic stem cells, trophoblast stem cells and induced extraembryonic endoderm stem cells closely recapitulate the development of wild-type and mutant natural mouse embryos up to embryonic day 8.5.

    • Gianluca Amadei
    • , Charlotte E. Handford
    •  & Magdalena Zernicka-Goetz

  • Article |

    3D transcriptomes reveal the molecular code of lineage specification in the primate embryo and provide an in vivo reference to decipher human development.

    • Sophie Bergmann
    • , Christopher A. Penfold
    •  & Thorsten E. Boroviak

  • Article
    | Open Access

    Blastoids derived from naive PXGL-cultured human pluripotent stem cells in which Hippo, TGF-β and ERK pathways are inhibited closely recapitulate aspects of blastocyst development, form cells resembling blastocyst-stage cells and thus provide a model system for implantation and development studies.

    • Harunobu Kagawa
    • , Alok Javali
    •  & Nicolas Rivron

  • Article |

    Depletion of TRF2—an essential mediator of telomere protection in most mammalian cells—in mouse embryonic stem cells activates a compensatory transcriptional program that renders TRF2 dispensable for their survival and proliferation.

    • Marta Markiewicz-Potoczny
    • , Anastasia Lobanova
    •  & Eros Lazzerini Denchi

  • Article |

    Single-cell RNA sequencing and spatial transcriptomics reveal that the somitogenesis clock is active in mouse gastruloids, which can be induced to generate somites with the correct rostral–caudal patterning.

    • Susanne C. van den Brink
    • , Anna Alemany
    •  & Alexander van Oudenaarden

  • Article |

    ERK reversibly regulates embryonic stem cell transcription via selective redistribution of co-factors and RNA polymerase from pluripotency to early differentiation enhancers, while leaving transcription factors bound to their enhancers, thus preserving plasticity.

    • William B. Hamilton
    • , Yaron Mosesson
    •  & Joshua M. Brickman

  • Letter |

    The transcription factor OTX2 ensures that germline induction is initially kept in check and only proceeds after OTX2 downregulation.

    • Jingchao Zhang
    • , Man Zhang
    •  & Ian Chambers

  • Letter |

    Stimulation of Wnt and Nodal pathways in micropatterned human embryonic stem cell colonies induce these colonies to exhibit characteristic spatial expression patterns of the organizer and reproduce organizer function when grafted into a host embryo.

    • I. Martyn
    • , T. Y. Kanno
    •  & A. H. Brivanlou

  • Letter |

    Trophoblast and embryonic stem cells interact in vitro to form structures that resemble early blastocysts, and the embryo provides signals that drive early trophectoderm development and implantation.

    • Nicolas C. Rivron
    • , Javier Frias-Aldeguer
    •  & Niels Geijsen

  • Letter |

    Exit of epiblasts from an unrestricted naive pluripotent state is required for epithelialization and generation of the pro-amniotic cavity in mouse embryos and for amniotic cavity formation in human embryos and human embryonic stem cells.

    • Marta N. Shahbazi
    • , Antonio Scialdone
    •  & Magdalena Zernicka-Goetz

  • Letter |

    Cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells, highlighting the feasibility of establishing expanded potential stem cells for other mammalian species.

    • Jian Yang
    • , David J. Ryan
    •  & Pentao Liu

  • Letter |

    The authors surveyed whole-exome and RNA-sequencing data from 252 unique pluripotent stem cell lines, some of which are in the pipeline for clinical use, and found that approximately 5% of cell lines had acquired mutations in the TP53 gene that allow mutant cells to rapidly outcompete non-mutant cells, but do not prevent differentiation.

    • Florian T. Merkle
    • , Sulagna Ghosh
    •  & Kevin Eggan

  • Article |

    A technique called genome architecture mapping (GAM) involves sequencing DNA from a large number of thin nuclear cryosections to develop a map of genome organization without the limitations of existing 3C-based methods.

    • Robert A. Beagrie
    • , Antonio Scialdone
    •  & Ana Pombo

  • Letter |

    Inhibition of mechanistic target of rapamycin (mTOR) suspends mouse blastocyst development and the cells remain ‘paused’ in a reversible pluripotent state, allowing prolonged culture.

    • Aydan Bulut-Karslioglu
    • , Steffen Biechele
    •  & Miguel Ramalho-Santos

  • Letter |

    Using a protocol that recapitulates both meiosis and oocyte growth in vitro, the authors induce mouse pluripotent stem cells to differentiate into fully functional oocytes that can be fertilized and generate viable offspring, thereby recapitulating the full mammalian female germline cycle in a dish.

    • Orie Hikabe
    • , Nobuhiko Hamazaki
    •  & Katsuhiko Hayashi

  • Article |

    The prevalence of N6-adenine DNA methylation in mammals was previously unknown; this study reveals that N6-methyladenine can be found in mouse embryonic stem cells, especially at subfamilies of young (<1.5 million years old) LINE-1 transposons.

    • Tao P. Wu
    • , Tao Wang
    •  & Andrew Z. Xiao

  • Letter |

    Haploid human embryonic stem cells have been derived from haploid oocytes, the cells maintain a normal haploid karyotype as pluripotent cells and, unexpectedly, as differentiated cells — loss-of-function genetic screens previously performed with haploid embryonic stem cells in mice can now be performed in humans.

    • Ido Sagi
    • , Gloryn Chia
    •  & Nissim Benvenisty

  • Letter |

    A differentiation protocol to obtain enteric nervous system (ENS) progenitors and a range of neurons from human pluripotent stem cells is developed; the cells can migrate and graft to the colon of a chick embryo and an adult mouse colon, including in a mouse model of Hirschsprung disease, in which a functional rescue is observed.

    • Faranak Fattahi
    • , Julius A Steinbeck
    •  & Lorenz Studer

  • Review Article |

    In response to the need for a defined set of criteria to assess stem-cell potency, this review proposes guidelines for the evaluation of newly derived pluripotent stem cells, from functional assays to integrative molecular analyses of transcriptional, epigenetic and metabolic states.

    • Alejandro De Los Angeles
    • , Francesco Ferrari
    •  & George Q. Daley

  • Letter |

    This study shows that a vertebrate-specific ubiquitin ligase modulates neural crest specification in Xenopus development and human embryonic stem-cell differentiation; a proteomics approach reveals that the CUL3KBTBD8 ligase modulates translation by targeting the modulators of ribosomes production NOLC1 and its paralogue TCOF1, which is mutated in a neural-crest-associated syndrome.

    • Achim Werner
    • , Shintaro Iwasaki
    •  & Michael Rape

  • Article
    | Open Access

    An analysis of genome-wide chromatin interactions during human embryonic stem cell differentiation reveals changes in chromatic organization and simultaneously identifies allele-resolved chromatin structure and differences in gene expression during differentiation.

    • Jesse R. Dixon
    • , Inkyung Jung
    •  & Bing Ren

  • Article |

    This study uses single-cell expression profiling of pluripotent stem cells after various perturbations, and uncovers a high degree of variability that can be inherited through cell divisions—modulating microRNA or external signalling pathways induces a ground state with reduced gene expression heterogeneity and a distinct chromatin profile.

    • Roshan M. Kumar
    • , Patrick Cahan
    •  & James J. Collins

  • Letter |

    The in vitro generation, from pluripotent stem cells, of three-dimensional human gastric organoids (hGOs) that contain a physiological gastric epithelium comprising both progenitor and differentiated cell types, and have expected functional characteristics is described, as is modelling the pathophysiological response of the human stomach to Helicobacter pylori using these hGOs.

    • Kyle W. McCracken
    • , Emily M. Catá
    •  & James M. Wells

  • Letter |

    The structure of mouse Dis3l2 bound to an oligoU substrate shows a funnel-like substrate-binding site with the RNA being fed into the active site along a path that is distinct from that seen in the related catalytic subunit of the exosome — 12 uracils of the oligoU-tailed RNA are recognized in a complex network of interactions, suggesting the basis for target specificity.

    • Christopher R. Faehnle
    • , Jack Walleshauser
    •  & Leemor Joshua-Tor

  • Letter |

    The ubiquitin ligase RLIM is known to activate the long non-coding RNA Xist, which is crucial for X-chromosome inactivation in female mice; however, unlike imprinted X-chromosome inactivation that requires RLIM for Xist expression, evidence is now provided that during random X-chromosome inactivation Xist expression is regulated by an RLIM-independent pathway in vivo.

    • JongDae Shin
    • , Mary C. Wallingford
    •  & Ingolf Bach

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