Molecular biology

  • Article
    | Open Access

    In S. pombe, small non-coding RNA mediates heterochromatin formation by recruiting the nucleosome remodeling and histone deacetylase complex. Here, the authors show that fly nucleosome remodeler Mi-2 and histone deacetylase Rpd3 are involved in piRNA-dependent transcriptional silencing of transposable elements.

    • Bruno Mugat
    • , Simon Nicot
    • , Carolina Varela-Chavez
    • , Christophe Jourdan
    • , Kaoru Sato
    • , Eugenia Basyuk
    • , François Juge
    • , Mikiko C. Siomi
    • , Alain Pélisson
    •  & Séverine Chambeyron
  • Article
    | Open Access

    REV7 has emerged as a critical regulator of DNA double-strand breaks repair. Here, the authors show that REV7 is crucial for both antibody class switch recombination and somatic hypermutation in activated B cells, in addition to their survival upon AID-deamination.

    • Dingpeng Yang
    • , Ying Sun
    • , Jingjing Chen
    • , Ying Zhang
    • , Shuangshuang Fan
    • , Min Huang
    • , Xia Xie
    • , Yanni Cai
    • , Yafang Shang
    • , Tuantuan Gui
    • , Liming Sun
    • , Jiazhi Hu
    • , Junchao Dong
    • , Leng-Siew Yeap
    • , Xiaoming Wang
    • , Wei Xiao
    •  & Fei-Long Meng
  • Article
    | Open Access

    Mediator is a multiprotein complex required to activate gene transcription by RNAPII. Here, the authors report that MED12 and MED13 are conditional positive regulators that facilitate the expression of genes depleted in active chromatin marks and the induction of gene expression in response to environmental stimuli in Arabidopsis.

    • Qikun Liu
    • , Sylvain Bischof
    • , C. Jake Harris
    • , Zhenhui Zhong
    • , Lingyu Zhan
    • , Calvin Nguyen
    • , Andrew Rashoff
    • , William D. Barshop
    • , Fei Sun
    • , Suhua Feng
    • , Magdalena Potok
    • , Javier Gallego-Bartolome
    • , Jixian Zhai
    • , James A. Wohlschlegel
    • , Michael F. Carey
    • , Jeffrey A. Long
    •  & Steven E. Jacobsen
  • Article
    | Open Access

    Previous study identified in vivo structured mRNA regions in Saccharomyces cerevisiae by dimethyl sulfate-sequencing. Here the authors use quantitative proteomics to identify protein interactors of 186 RNA folds in S. cerevisiae, providing functional links between RNA binding proteins and distinct mRNA fold.

    • Nuria Casas-Vila
    • , Sergi Sayols
    • , Lara Pérez-Martínez
    • , Marion Scheibe
    •  & Falk Butter
  • Article
    | Open Access

    Small molecule polyamines participate in diverse aspects of cell growth and differentiation and are known to regulate ion channel gating. Here authors reveal that cellular polyamines control nicotinic acetylcholine receptor (nAChR) biogenesis, and either catabolic degradation or inhibition of polyamine production augments nAChR assembly.

    • Madhurima Dhara
    • , Jose A. Matta
    • , Min Lei
    • , Daniel Knowland
    • , Hong Yu
    • , Shenyan Gu
    •  & David S. Bredt
  • Article
    | Open Access

    Riboswitches consist of an aptamer domain and expression platform, which senses a signal and regulates gene expression, respectively. Here the authors show that the expression platform of a SAM-I riboswitch from the Gram-negative bacteria can sense and bind uncharged an initiator Met tRNA to control the met operon.

    • Dong-Jie Tang
    • , Xinyu Du
    • , Qiang Shi
    • , Jian-Ling Zhang
    • , Yuan-Ping He
    • , Yan-Miao Chen
    • , Zhenhua Ming
    • , Dan Wang
    • , Wan-Ying Zhong
    • , Yu-Wei Liang
    • , Jin-Yang Liu
    • , Jian-Ming Huang
    • , Yun-Shi Zhong
    • , Shi-Qi An
    • , Hongzhou Gu
    •  & Ji-Liang Tang
  • Article
    | Open Access

    3′ end of microRNAs binds to the PAZ domain of Argonaute (AGO) proteins. Here the authors show that terminal nucleotidyltransferases TUT4/7 and exonuclease DIS3L2 induce tailing and decay of 3’ end exposed-microRNAs in AGO PAZ mutant expressing- or cancer cells.

    • Acong Yang
    • , Tie-Juan Shao
    • , Xavier Bofill-De Ros
    • , Chuanjiang Lian
    • , Patricia Villanueva
    • , Lisheng Dai
    •  & Shuo Gu
  • Article
    | Open Access

    Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.

    • Javier Santos-Moreno
    • , Eve Tasiudi
    • , Joerg Stelling
    •  & Yolanda Schaerli
  • Article
    | Open Access

    The MAF bZIP transcription factor B (MAFB) is present in postnatal human beta cells but its role is unclear. Here, the authors show that MAFB regulates endocrine pancreatic cell fate specification.

    • Ronan Russell
    • , Phichitpol P. Carnese
    • , Thomas G. Hennings
    • , Emily M. Walker
    • , Holger A. Russ
    • , Jennifer S. Liu
    • , Simone Giacometti
    • , Roland Stein
    •  & Matthias Hebrok
  • Article
    | Open Access

    The mechanism underlying periodicity of Plasmodium’s intra-erythrocytic developmental cycle (IDC) is unclear. Here, Subudhi et al. show that serpentine receptor 10 (SR10) plays a role in regulating the schedule of the IDC in line with the timing of host daily rhythms.

    • Amit K. Subudhi
    • , Aidan J. O’Donnell
    • , Abhinay Ramaprasad
    • , Hussein M. Abkallo
    • , Abhinav Kaushik
    • , Hifzur R. Ansari
    • , Alyaa M. Abdel-Haleem
    • , Fathia Ben Rached
    • , Osamu Kaneko
    • , Richard Culleton
    • , Sarah E. Reece
    •  & Arnab Pain
  • Article
    | Open Access

    The bacterial effector MavC can ubiquitinate the host E2 enzyme UBE2N to dampen the host immune response. Here, the authors provide mechanistic insight into this non-canonical ubiquitination machinery and reveal the structural basis for the functional differences between MavC and its close homolog MvcA.

    • Yong Wang
    • , Qi Zhan
    • , Xinlu Wang
    • , Peipei Li
    • , Songqing Liu
    • , Guangxia Gao
    •  & Pu Gao
  • Article
    | Open Access

    Mitochondrial oxidative phosphorylation produces ATP and is an important source for cellular energy equivalents. Here the authors perform a cell-based screen to identify genes that alleviate perturbed mitochondrial complex I function and identify malic enzyme (ME-1), which promotes survival by production of NADPH and glutathione.

    • Eduardo Balsa
    • , Elizabeth A. Perry
    • , Christopher F. Bennett
    • , Mark Jedrychowski
    • , Steven P. Gygi
    • , John G. Doench
    •  & Pere Puigserver
  • Article
    | Open Access

    The relationship between regulatory elements, chromatin interactions and gene expression during development remains poorly understood. Here the authors present Tiled-C, a low-input 3C approach to study genome architecture at high resolution, and apply it to mouse erythroid differentiation in vivo, finding that enhancer-promoter interactions are formed gradually during differentiation, concomitant with progressive upregulation of gene activity.

    • A. Marieke Oudelaar
    • , Robert A. Beagrie
    • , Matthew Gosden
    • , Sara de Ornellas
    • , Emily Georgiades
    • , Jon Kerry
    • , Daniel Hidalgo
    • , Joana Carrelha
    • , Arun Shivalingam
    • , Afaf H. El-Sagheer
    • , Jelena M. Telenius
    • , Tom Brown
    • , Veronica J. Buckle
    • , Merav Socolovsky
    • , Douglas R. Higgs
    •  & Jim R. Hughes
  • Article
    | Open Access

    Long noncoding RNA SAM (Sugt1 associated muscle) is upregulated in the proliferating myoblast cells. Here the authors investigate SAM knockout mice and suggest that SAM binds and stabilizes Sugt1, a co-chaperone protein that regulates kinetochore assembly.

    • Yuying Li
    • , Jie Yuan
    • , Fengyuan Chen
    • , Suyang Zhang
    • , Yu Zhao
    • , Xiaona Chen
    • , Leina Lu
    • , Liang Zhou
    • , Ching Yan Chu
    • , Hao Sun
    •  & Huating Wang
  • Article
    | Open Access

    Local activity of the DNA methylation machinery remains poorly understood. Here, the authors present a theoretical and experimental framework to infer methylation and demethylation rates at genome scale in mouse embryonic stem cells, finding that maintenance methylation activity is reduced at transcription factor binding sites, while methylation turnover is elevated in transcribed gene bodies.

    • Paul Adrian Ginno
    • , Dimos Gaidatzis
    • , Angelika Feldmann
    • , Leslie Hoerner
    • , Dilek Imanci
    • , Lukas Burger
    • , Frederic Zilbermann
    • , Antoine H. F. M. Peters
    • , Frank Edenhofer
    • , Sébastien A. Smallwood
    • , Arnaud R. Krebs
    •  & Dirk Schübeler
  • Article
    | Open Access

    mRNA translation efficiency is regulated in response to stimuli. Here the authors employ mass spectrometry analysis of ribosome fractions and show that under hypoxia, oxygen-sensitive RNA binding proteins enhance the translation efficiency of glycolysis pathway transcripts.

    • J. J. David Ho
    • , Nathan C. Balukoff
    • , Phaedra R. Theodoridis
    • , Miling Wang
    • , Jonathan R. Krieger
    • , Jonathan H. Schatz
    •  & Stephen Lee
  • Article
    | Open Access

    Until now, the transcriptome of preimplantation mouse embryos has only been analysed by short-read sequencing. Here, the authors perform long-read sequencing to provide a more detailed transcriptome of the preimplantation mouse embryo, identifying various novel transcripts, for example Kdm4dl.

    • Yunbo Qiao
    • , Chao Ren
    • , Shisheng Huang
    • , Jie Yuan
    • , Xingchen Liu
    • , Jiao Fan
    • , Jianxiang Lin
    • , Susu Wu
    • , Qiuzhen Chen
    • , Xiaochen Bo
    • , Xiangyang Li
    • , Xingxu Huang
    • , Zhen Liu
    •  & Wenjie Shu
  • Article
    | Open Access

    Mammary epithelial cells are epigenetically modified during pregnancy, these changes can influence the pre-disposition to cancer. Here, the authors examine the epigenetic landscape of mammary epithelial cells pre and post pregnancy and identify changes to the epigenetic landscape, which can protect mice from Myc induced cancer.

    • Mary J. Feigman
    • , Matthew A. Moss
    • , Chen Chen
    • , Samantha L. Cyrill
    • , Michael F. Ciccone
    • , Marygrace C. Trousdell
    • , Shih-Ting Yang
    • , Wesley D. Frey
    • , John E. Wilkinson
    •  & Camila O. dos Santos
  • Article
    | Open Access

    The DIS3L2 exonuclease degrades aberrant 7SL RNAs tagged by an oligouridine 3′-tail. Here the authors analyze DIS3L2 knockout mouse embryonic stem cells and suggest that DIS3L2-mediated quality control of 7SL RNA is important for ER-mediated translation and calcium ion homeostasis.

    • Mehdi Pirouz
    • , Chih-Hao Wang
    • , Qi Liu
    • , Aref G. Ebrahimi
    • , Farnaz Shamsi
    • , Yu-Hua Tseng
    •  & Richard I. Gregory
  • Article
    | Open Access

    Over 100 million of opioid prescriptions are issued yearly in the USA alone, but the impact of opioid use on the immune system is barely characterized. Here the authors report antiviral immune response is blunted in several types of blood cells from opioid-dependent individuals, and when healthy donor cells are exposed to morphine in a dish.

    • Tanya T. Karagiannis
    • , John P. Cleary Jr
    • , Busra Gok
    • , Andrew J. Henderson
    • , Nicholas G. Martin
    • , Masanao Yajima
    • , Elliot C. Nelson
    •  & Christine S. Cheng
  • Article
    | Open Access

    Acquired resistance limits the efficacy of PARP inhibitors (PARPi) in high grade serous ovarian cancer (HGSOC). Here, the authors show that inhibition of RNA polymerase I transcription using CX-5461 increases the therapeutic efficacy of PARPi and overcomes PARPi resistance in PDX models of HGSOC.

    • Elaine Sanij
    • , Katherine M. Hannan
    • , Jiachen Xuan
    • , Shunfei Yan
    • , Jessica E. Ahern
    • , Anna S. Trigos
    • , Natalie Brajanovski
    • , Jinbae Son
    • , Keefe T. Chan
    • , Olga Kondrashova
    • , Elizabeth Lieschke
    • , Matthew J. Wakefield
    • , Daniel Frank
    • , Sarah Ellis
    • , Carleen Cullinane
    • , Jian Kang
    • , Gretchen Poortinga
    • , Purba Nag
    • , Andrew J. Deans
    • , Kum Kum Khanna
    • , Linda Mileshkin
    • , Grant A. McArthur
    • , John Soong
    • , Els M. J. J. Berns
    • , Ross D. Hannan
    • , Clare L. Scott
    • , Karen E. Sheppard
    •  & Richard B. Pearson
  • Article
    | Open Access

    The bromodomain containing protein BRD9 has been reported to regulate chromatin remodeling and transcription. Here the authors reveal a role for BRD9 in homologous recombination by facilitating RAD51–RAD54 interaction.

    • Qin Zhou
    • , Jinzhou Huang
    • , Chao Zhang
    • , Fei Zhao
    • , Wootae Kim
    • , Xinyi Tu
    • , Yong Zhang
    • , Somaira Nowsheen
    • , Qian Zhu
    • , Min Deng
    • , Yuping Chen
    • , Bo Qin
    • , Kuntian Luo
    • , Baohua Liu
    • , Zhenkun Lou
    • , Robert W. Mutter
    •  & Jian Yuan
  • Article
    | Open Access

    The relationship between histopathology, gene expression, and biochemical and mechanical properties of wounds is largely unknown. Here, the authors show that activin A alters wound healing at multiple levels by promoting pro-fibrotic gene expression and matrix deposition, thereby affecting biomechanical properties of skin wounds.

    • Mateusz S. Wietecha
    • , Marco Pensalfini
    • , Michael Cangkrama
    • , Bettina Müller
    • , Juyoung Jin
    • , Jürgen Brinckmann
    • , Edoardo Mazza
    •  & Sabine Werner
  • Article
    | Open Access

    While DNA methylation is thought to play a regulatory role, there are few examples where modification of a single CpG dinucleotide directly affects transcription factor binding. Here the authors show that methylation of a single CGATA element within the c-Kit gene inhibits binding and regulation by erythroid transcription factor GATA-1, both in cells and in mice, suggesting that methylation at this site plays an essential role in erythropoiesis.

    • Lu Yang
    • , Zhiliang Chen
    • , Elizabeth S. Stout
    • , Fabien Delerue
    • , Lars M. Ittner
    • , Marc R. Wilkins
    • , Kate G. R. Quinlan
    •  & Merlin Crossley
  • Article
    | Open Access

    In tumors, hundreds of genes can undergo loss of heterozygosity (LOH). Here, the authors investigate the potential for this LOH as a class of non-driver cancer vulnerabilities.

    • Caitlin A. Nichols
    • , William J. Gibson
    • , Meredith S. Brown
    • , Jack A. Kosmicki
    • , John P. Busanovich
    • , Hope Wei
    • , Laura M. Urbanski
    • , Naomi Curimjee
    • , Ashton C. Berger
    • , Galen F. Gao
    • , Andrew D. Cherniack
    • , Sirano Dhe-Paganon
    • , Brenton R. Paolella
    •  & Rameen Beroukhim
  • Article
    | Open Access

    METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. Here the authors show that DALR anticodon binding domain containing 3 (DALRD3) forms a complex with METTL2 to recognize specific arginine tRNAs and find DALRD3 mutations in patients with developmental delay and early-onset epileptic encephalopathy.

    • Jenna M. Lentini
    • , Hessa S. Alsaif
    • , Eissa Faqeih
    • , Fowzan S. Alkuraya
    •  & Dragony Fu
  • Article
    | Open Access

    Chronic lung diseases are characterized by molecular and cellular composition changes. Here the authors use single-cell RNA sequencing to map cell type-specific changes in human tracheal epithelium related to smoking, and to provide evidence for a tuft-like progenitor for pulmonary neuroendocrine cells and ionocytes.

    • Katherine C. Goldfarbmuren
    • , Nathan D. Jackson
    • , Satria P. Sajuthi
    • , Nathan Dyjack
    • , Katie S. Li
    • , Cydney L. Rios
    • , Elizabeth G. Plender
    • , Michael T. Montgomery
    • , Jamie L. Everman
    • , Preston E. Bratcher
    • , Eszter K. Vladar
    •  & Max A. Seibold
  • Article
    | Open Access

    Pioneer factors direct cell fate through switching inaccessible chromatin to an accessible state at specific target enhancers. Here the authors show that HOX13 transcription factors have a pioneer activity which is required for the proper implementation of the distal limb developmental program.

    • Ines Desanlis
    • , Yacine Kherdjemil
    • , Alexandre Mayran
    • , Yasser Bouklouch
    • , Claudia Gentile
    • , Rushikesh Sheth
    • , Rolf Zeller
    • , Jacques Drouin
    •  & Marie Kmita
  • Article
    | Open Access

    Histone ubiquitination plays a critical role in the DNA damage response pathway. Here the authors reveal how RNF168 ubiquitinates the H2A family including noncanonical variants, H2AZ and macroH2A1/2, at the divergent N-terminal tail lysine residue.

    • Jessica L. Kelliher
    • , Kirk L. West
    • , Qingguo Gong
    •  & Justin W. C. Leung
  • Article
    | Open Access

    Defects in DNA repair have been linked to brain aging and neurodegenerative disorders. Here the authors reveal a role for HDAC1 in stimulating OGG1 activity to alleviate 8-oxoG lesions with implications in the aging brain and neurodegenerative diseases.

    • Ping-Chieh Pao
    • , Debasis Patnaik
    • , L. Ashley Watson
    • , Fan Gao
    • , Ling Pan
    • , Jun Wang
    • , Chinnakkaruppan Adaikkan
    • , Jay Penney
    • , Hugh P. Cam
    • , Wen-Chin Huang
    • , Lorena Pantano
    • , Audrey Lee
    • , Alexi Nott
    • , Trongha X. Phan
    • , Elizabeta Gjoneska
    • , Sara Elmsaouri
    • , Stephen J. Haggarty
    •  & Li-Huei Tsai
  • Article
    | Open Access

    Characterization of the distance over which TF binding influences gene expression is important for inferring target genes. Here the authors study this relationship using thousands of genomic data sets, finding two classes of TFs with distinct ranges of regulatory influence modulated by chromatin states of topologically associated domains.

    • Chen-Hao Chen
    • , Rongbin Zheng
    • , Collin Tokheim
    • , Xin Dong
    • , Jingyu Fan
    • , Changxin Wan
    • , Qin Tang
    • , Myles Brown
    • , Jun S. Liu
    • , Clifford A. Meyer
    •  & X. Shirley Liu
  • Article
    | Open Access

    DNA polymerase epsilon (Pol ε) synthesizes half of the genome in every cell cycle. Here the authors uncover a dual role of a unique domain within the Pol ε catalytic core in replisome assembly and DNA synthesis and the impact of this domain on genome stability.

    • Xiangzhou Meng
    • , Lei Wei
    • , Sujan Devbhandari
    • , Tuo Zhang
    • , Jenny Xiang
    • , Dirk Remus
    •  & Xiaolan Zhao
  • Review Article
    | Open Access

    Our efforts to build complex synthetic biology circuits are impeded by limited knowledge of optimal combinations. In this review, the authors consider current combinatorial methods and look to emerging technologies.

    • Gita Naseri
    •  & Mattheos A. G. Koffas
  • Article
    | Open Access

    Ribonucleoside monophosphates are incorporated by DNA polymerases into double-stranded DNA. Here, the authors use ribose-seq and Ribose-Map techniques to reveal that signatures and patterns of ribonucleotide incorporation in yeast mitochondrial and nuclear DNA show preference for cytosine and guanosine preceded by deoxyadenosine.

    • Sathya Balachander
    • , Alli L. Gombolay
    • , Taehwan Yang
    • , Penghao Xu
    • , Gary Newnam
    • , Havva Keskin
    • , Waleed M. M. El-Sayed
    • , Anton V. Bryksin
    • , Sijia Tao
    • , Nicole E. Bowen
    • , Raymond F. Schinazi
    • , Baek Kim
    • , Kyung Duk Koh
    • , Fredrik O. Vannberg
    •  & Francesca Storici
  • Article
    | Open Access

    Identification of low abundance proteins interacting with viral proteins can be challenging. Here, Du et al. develop an mRNA display approach with a library of even distribution, identify host proteins interacting with NS1 protein of influenza A virus, and show that one interactor provides a means to regulate cellular fatty acids synthesis.

    • Yushen Du
    • , Judd F. Hultquist
    • , Quan Zhou
    • , Anders Olson
    • , Yenwen Tseng
    • , Tian-hao Zhang
    • , Mengying Hong
    • , Kejun Tang
    • , Liubo Chen
    • , Xiangzhi Meng
    • , Michael J. McGregor
    • , Lei Dai
    • , Danyang Gong
    • , Laura Martin-Sancho
    • , Sumit Chanda
    • , Xinming Li
    • , Steve Bensenger
    • , Nevan J. Krogan
    •  & Ren Sun
  • Article
    | Open Access

    Transcription noise in bacteria is often attributed to burstiness, but the mechanisms are unclear. Here, the authors show that the transition from low to high expression can be regulated via burst size or burst frequency, depending on the mode of transcription initiation determined by different sigma factors.

    • Christoph Engl
    • , Goran Jovanovic
    • , Rowan D. Brackston
    • , Ioly Kotta-Loizou
    •  & Martin Buck
  • Article
    | Open Access

    In fission yeast, several lncRNAs act in cis to regulate expression of adjacent genes. Here, the authors show that the conserved Pir2ARS2 protein is targeted, along with splicing factors, to cryptic introns in lncRNAs and recruits effectors, including RNAi machinery, for gene repression.

    • Gobi Thillainadesan
    • , Hua Xiao
    • , Sahana Holla
    • , Jothy Dhakshnamoorthy
    • , Lisa M. Miller Jenkins
    • , David Wheeler
    •  & Shiv I. S. Grewal
  • Article
    | Open Access

    IRE1α plays a key role in the unfolded protein response (UPR) by promoting the unconventional splicing of the XBP1 and the selective cleavage of RNAs. Here the authors report that IRE1α is activated upon the DNA damage response and selectively controls the stability of mRNAs to maintain genome integrity.

    • Estefanie Dufey
    • , José Manuel Bravo-San Pedro
    • , Cristian Eggers
    • , Matías González-Quiroz
    • , Hery Urra
    • , Alfredo I. Sagredo
    • , Denisse Sepulveda
    • , Philippe Pihán
    • , Amado Carreras-Sureda
    • , Younis Hazari
    • , Eduardo A. Sagredo
    • , Daniela Gutierrez
    • , Cristian Valls
    • , Alexandra Papaioannou
    • , Diego Acosta-Alvear
    • , Gisela Campos
    • , Pedro M. Domingos
    • , Rémy Pedeux
    • , Eric Chevet
    • , Alejandra Alvarez
    • , Patricio Godoy
    • , Peter Walter
    • , Alvaro Glavic
    • , Guido Kroemer
    •  & Claudio Hetz
  • Article
    | Open Access

    Due to the polygenic nature of most diseases, simultaneous correction or introduction of single nucleotide variants is needed. Here, the authors demonstrated the feasibility of multiplex base editing for polygenes disease modeling in cynomolgus monkey embryos with high specificity.

    • Wenhui Zhang
    • , Tomomi Aida
    • , Ricardo C. H. del Rosario
    • , Jonathan J. Wilde
    • , Chenhui Ding
    • , Xiaohui Zhang
    • , Zulqurain Baloch
    • , Yan Huang
    • , Yu Tang
    • , Duanduan Li
    • , Hongyu Lu
    • , Yang Zhou
    • , Minqing Jiang
    • , Dongdong Xu
    • , Zhihao Fang
    • , Zhanhong Zheng
    • , Qunshan Huang
    • , Guoping Feng
    •  & Shihua Yang
  • Article
    | Open Access

    In budding yeast, the decision to enter meiosis is defined by nutrient and mating-type signals regulating expression of the master transcription factor for meiotic entry, IME1. Here, the authors characterize the steps involved in regulating the entering in meiosis via the co-repressor complex Tup1-Cyc8.

    • Janis Tam
    •  & Folkert J. van Werven
  • Article
    | Open Access

    Early life exposure to environmental stressors, including endocrine disrupting chemicals (EDCs), can impact health later in life. Here, the authors show that neonatal EDC exposure in rats causes epigenetic reprogramming in the liver, which is transcriptionally silent until animals are placed on a Western-style diet.

    • Lindsey S. Treviño
    • , Jianrong Dong
    • , Ahkilesh Kaushal
    • , Tiffany A. Katz
    • , Rahul Kumar Jangid
    • , Matthew J. Robertson
    • , Sandra L. Grimm
    • , Chandra Shekar R. Ambati
    • , Vasanta Putluri
    • , Aaron R. Cox
    • , Kang Ho Kim
    • , Thaddeus D. May
    • , Morgan R. Gallo
    • , David D. Moore
    • , Sean M. Hartig
    • , Charles E. Foulds
    • , Nagireddy Putluri
    • , Cristian Coarfa
    •  & Cheryl Lyn Walker
  • Article
    | Open Access

    Beta-adrenergic stimulation of brown adipose tissue leads to thermogenesis via the activating transcription factor 2 (ATF2) mediated expression of the thermogenic genes Ucp1 and Pgc-1α. Here, the authors show that the scaffold protein p62 regulates brown adipose tissue function through modifying ATF2 genomic binding and subsequent Ucp1 and Pgc-1α induction.

    • Katrin Fischer
    • , Anna Fenzl
    • , Dianxin Liu
    • , Kenneth A. Dyar
    • , Maximilian Kleinert
    • , Markus Brielmeier
    • , Christoffer Clemmensen
    • , Anna Fedl
    • , Brian Finan
    • , Andre Gessner
    • , Martin Jastroch
    • , Jianfeng Huang
    • , Susanne Keipert
    • , Martin Klingenspor
    • , Jens C. Brüning
    • , Manfred Kneilling
    • , Florian C. Maier
    • , Ahmed E. Othman
    • , Bernd J. Pichler
    • , Ines Pramme-Steinwachs
    • , Stephan Sachs
    • , Angelika Scheideler
    • , Wolfgang M. Thaiss
    • , Henriette Uhlenhaut
    • , Siegfried Ussar
    • , Stephen C. Woods
    • , Julia Zorn
    • , Kerstin Stemmer
    • , Sheila Collins
    • , Maria Diaz-Meco
    • , Jorge Moscat
    • , Matthias H. Tschöp
    •  & Timo D. Müller
  • Article
    | Open Access

    The MOF acetyltransferase-containing Non-Specific Lethal (NSL) complex is a broad transcription regulator and haploinsufficiency of its KANSL1 subunit results in the Koolen-de Vries syndrome in humans. Here, the authors identify the BET protein BRD4 as evolutionary conserved co-factor of the NSL complex and provide evidence that NSL-deposited histone acetylation induces BRD4 recruitment for transcription of constitutively active genes.

    • Aline Gaub
    • , Bilal N. Sheikh
    • , M. Felicia Basilicata
    • , Marie Vincent
    • , Mathilde Nizon
    • , Cindy Colson
    • , Matthew J. Bird
    • , James E. Bradner
    • , Julien Thevenon
    • , Michael Boutros
    •  & Asifa Akhtar
  • Article
    | Open Access

    WUSCHEL is a homeodomain transcription factor that is essential for stem cell maintenance in the plant shoot apical meristem. Here, via structural and biochemical approaches, Sloan et al. show that strong WUSCHEL binding to preferential target motifs can be attributed to dimer formation that stabilizes DNA binding.

    • Jeremy Sloan
    • , Jana P. Hakenjos
    • , Michael Gebert
    • , Olga Ermakova
    • , Andrea Gumiero
    • , Gunter Stier
    • , Klemens Wild
    • , Irmgard Sinning
    •  & Jan U. Lohmann
  • Article
    | Open Access

    ATRX is an RNA binding protein that mediates targeting of polycomb repressive complex 2 (PRC2) to genomic sites. Here the authors identify the RNA binding region and show that the RNA binding is required for ATRX localization and for its recruitment of PRC2 to a subset of polycomb targets.

    • Wenqing Ren
    • , Nicole Medeiros
    • , Robert Warneford-Thomson
    • , Phillip Wulfridge
    • , Qingqing Yan
    • , Joyce Bian
    • , Simone Sidoli
    • , Benjamin A. Garcia
    • , Emmanuel Skordalakes
    • , Eric Joyce
    • , Roberto Bonasio
    •  & Kavitha Sarma
  • Article
    | Open Access

    The function on subgenomic flaviviral RNA (sfRNA) in the mosquito vector is not well understood. Here, Slonchak et al. show that sfRNA affects virus-induced apoptosis and dissemination of ZIKV in Aedes aegypti mosquitoes, suggesting a role of sfRNA in Zika virus replication and transmission.

    • Andrii Slonchak
    • , Leon E. Hugo
    • , Morgan E. Freney
    • , Sonja Hall-Mendelin
    • , Alberto A. Amarilla
    • , Francisco J. Torres
    • , Yin Xiang Setoh
    • , Nias Y. G. Peng
    • , Julian D. J. Sng
    • , Roy A. Hall
    • , Andrew F. van den Hurk
    • , Gregor J. Devine
    •  & Alexander A. Khromykh
  • Article
    | Open Access

    Methylation of histone H3 lysine 4 (H3K4) by Set1/COMPASS occurs co-transcriptionally, and is important for gene regulation. Here the authors provide evidence that the Set1 N-terminal region and the COMPASS subunit Swd2 interact with RNA polymerase II CTD to recruit COMPASS in budding yeast.

    • Hyun Jin Bae
    • , Marion Dubarry
    • , Jongcheol Jeon
    • , Luis M. Soares
    • , Catherine Dargemont
    • , Jaehoon Kim
    • , Vincent Geli
    •  & Stephen Buratowski