Featured
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Letter |
MHC class II tetramers engineered for enhanced binding to CD4 improve detection of antigen-specific T cells
The detection of antigen-specific CD4+ T cells is improved with engineered peptide:MHCII tetramers.
- Thamotharampillai Dileepan
- , Deepali Malhotra
- & Marc K. Jenkins
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Correspondence |
Animal-free alternatives and the antibody iceberg
- Alison Gray
- , Andrew R. M. Bradbury
- & Stefan Dübel
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Article |
Massively parallel interrogation and mining of natively paired human TCRαβ repertoires
T cell receptors are identified in large-scale screening of T cell repertoires cloned from primary human T cells.
- Matthew J. Spindler
- , Ayla L. Nelson
- & David S. Johnson
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Article |
Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues
A DNA-based amplification scheme enables highly multiplexed immunofluorescence imaging
- Sinem K. Saka
- , Yu Wang
- & Peng Yin
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Letter |
T cell receptor fingerprinting enables in-depth characterization of the interactions governing recognition of peptide–MHC complexes
The relative affinity of T cell receptors for different peptide–MHCs is measured at high throughput, revealing T cell cross-reactivity.
- Amalie K Bentzen
- , Lina Such
- & Sine R Hadrup
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Article |
Enhancing T cell therapy through TCR-signaling-responsive nanoparticle drug delivery
Cytokines released after T cell activation improve the efficacy of T cell therapies in mice.
- Li Tang
- , Yiran Zheng
- & Darrell J Irvine
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Article |
Scaffolds that mimic antigen-presenting cells enable ex vivo expansion of primary T cells
T cells are readily expanded in culture using a system that presents membrane-bound and soluble cues in a natural context.
- Alexander S Cheung
- , David K Y Zhang
- & David J Mooney
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Brief Communication |
Functional interrogation and mining of natively paired human VH:VL antibody repertoires
Libraries of natively paired human VH:VL chains enable screening of the human B-cell repertoire in yeast.
- Bo Wang
- , Brandon J DeKosky
- & George Georgiou
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News & Views |
Tuning up T-cell receptors
T-cell receptors with higher affinity for tumor antigens are discovered by harnessing T-cell development.
- C Garrett Rappazzo
- & Michael E Birnbaum
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News & Views |
Mapping the secrets of the antibody pool
The progression of antibody-mediated immunity can now be monitored at high-throughput on a single-cell level.
- Jonathan R McDaniel
- , Gregory C Ippolito
- & George Georgiou
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Article |
Large-scale detection of antigen-specific T cells using peptide-MHC-I multimers labeled with DNA barcodes
Detection of more than 1,000 different T-cell specificities in parallel facilitates identification of cancer and autoimmune antigens.
- Amalie Kai Bentzen
- , Andrea Marion Marquard
- & Sine Reker Hadrup
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Letter |
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Improved expansion microscopy method preserves signal from fluorescent proteins and antibodies using off-the-shelf reagents.
- Paul W Tillberg
- , Fei Chen
- & Edward S Boyden
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Patents |
Recent patents in antibody engineering
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Editorial |
No sample left behind
Maximizing the clinical impact of immune profiling will require dedicated immune monitoring facilities and community standards for handling patient samples.
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Article |
Linking T-cell receptor sequence to functional phenotype at the single-cell level
Multiplexed single cell sequencing allows the identification of functional TCRα-TCRβ pairs
- Arnold Han
- , Jacob Glanville
- & Mark M Davis
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News & Views |
Of snowflakes and natural killer cell subsets
Mass cytometric analysis reveals a remarkable and unanticipated degree of phenotypic heterogeneity among human peripheral blood natural killer cells.
- Lewis L Lanier
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Review Article |
The promise and challenge of high-throughput sequencing of the antibody repertoire
Georgiou and colleagues discuss rapidly evolving methods for high-throughput sequencing of the antibody repertoire, and how the resulting data may be applied to answer basic and translational research questions.
- George Georgiou
- , Gregory C Ippolito
- & Stephen R Quake
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Article |
Nature-inspired design of motif-specific antibody scaffolds
Engineering motif-specific 'hot spots' into an antibody scaffold yields antibodies with high affinity to targets containing phosphoserine, phosphothreonine or phosphotyrosine.
- James T Koerber
- , Nathan D Thomsen
- & James A Wells
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Letter |
Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies
In a bacterial expression system, two half antibodies, each having distinct light chains, spontaneously assemble into an intact bispecific molecule with natural antibody architecture
- Christoph Spiess
- , Mark Merchant
- & Justin M Scheer
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Article |
A proteomics approach for the identification and cloning of monoclonal antibodies from serum
The ability to identify antibodies circulating in the bloodstream would advance immunology and vaccinology research and the development of therapeutics. Cheung et al. couple proteomics with next-generation sequencing of RNA from B cells to clone antibodies directly from the sera of immunized rabbits and mice.
- Wan Cheung Cheung
- , Sean A Beausoleil
- & Roberto D Polakiewicz
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Perspective |
Beyond natural antibodies: the power of in vitro display technologies
- Andrew R M Bradbury
- , Sachdev Sidhu
- & John McCafferty
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Editorial |
Similarity trials
A European guideline on biosimilar monoclonal antibodies suggests smaller trials with homogeneous, younger patient groups may suffice for marketing authorization.
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Article |
Spatially addressed combinatorial protein libraries for recombinant antibody discovery and optimization
Antibodies that modulate the activity of a target are difficult to discover with display-based approaches, which select only high-affinity binders. Mao et al. identify antibodies with a range of affinities using a small-molecule discovery method that involves one-by-one screening of an optimized small library of antibody fragments with known sequences.
- Hongyuan Mao
- , James J Graziano
- & Vaughn V Smider
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Letter |
Antibody recycling by engineered pH-dependent antigen binding improves the duration of antigen neutralization
The typical therapeutic antibody binds only two target antigen molecules during its lifetime. Igawa et al. describe a method for engineering antibody recycling in vivo, suggesting an approach to reduce the size and/or frequency of dosage with therapeutic antibodies.
- Tomoyuki Igawa
- , Shinya Ishii
- & Kunihiro Hattori
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Letter |
Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells
Isolation of antigen-specific antibodies or antibody fragments, whether through B-cell immortalization or recombinant libraries, generally requires laborious screening. Reddy et al. circumvent this step using high-throughput sequencing of plasma cells and bioinformatic analysis of the variable-gene repertoire.
- Sai T Reddy
- , Xin Ge
- & George Georgiou
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Letter |
Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations
Rissin et al. increase the sensitivity of sandwich ELISA by segregating beads bearing a single enzyme-labeled immunoconjugate into femtoliter-volume reaction chambers. As the small volume of each well permits detection of extremely low levels of fluorescence, protein abundance is determined by counting the number of fluorescent wells as a percentage of the number of wells containing beads.
- David M Rissin
- , Cheuk W Kan
- & David C Duffy
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