Electron microscopy articles within Nature Methods

Featured

  • Commentary |

    Cryo-EM has emerged rapidly as a method for determining high-resolution structures of biological macromolecules. The author of this Commentary discusses just how much better this technology may get and how fast such developments are likely to happen.

    • Robert M Glaeser

  • News Feature |

    Recent advances in cryo-electron microscopy are enabling researchers to solve protein structures at near-atomic resolutions, expanding the biological applicability of this technique. Michael Eisenstein reports.

    • Michael Eisenstein

  • Research Highlights |

    The highest-resolution structure solved by cryo-electron microscopy to date reveals what it takes to reach the resolution realm of X-ray crystallography.

    • Allison Doerr

  • Brief Communication |

    New detector technology has improved the resolution of cryo-electron microscopy (cryo-EM), but tools for structure determination from high-resolution maps have lagged behind. Wang et al. describe a de novo approach for structure determination from high-resolution cryo-EM maps. Also in this issue, DiMaio et al. report structure determination using a homologous structure as a starting model.

    • Ray Yu-Ruei Wang
    • , Mikhail Kudryashev
    •  & Frank DiMaio

  • Article |

    New detector technology has improved the resolution of cryo-electron microscopy (cryo-EM), but tools for structure determination from high-resolution maps have lagged behind. DiMaio et al. report structure determination from high-resolution cryo-EM maps using a homologous structure as a starting model. Also in this issue, Wang et al. describe a de novo approach for structure determination that does not require a starting model.

    • Frank DiMaio
    • , Yifan Song
    •  & David Baker

  • Research Highlights |

    Gold supports nearly eliminate unwanted specimen motion in cryo-electron microscopy, enabling high-resolution structure determination of challenging proteins.

    • Allison Doerr

  • Brief Communication |

    High-resolution, three-dimensional protein structures can be solved using MicroED, an electron diffraction method that uses three-dimensional microcrystals. An improved MicroED data collection approach described here increases data quality and resolution and extends its broad applicability.

    • Brent L Nannenga
    • , Dan Shi
    •  & Tamir Gonen

  • Brief Communication |

    Graphene is in many ways an ideal sample support for cryo-electron microscopy, but its hydrophobicity prevents adsorption of protein from aqueous solution. Low-energy hydrogen-plasma treatment renders graphene hydrophilic and enables controlled adsorption of protein to its surface.

    • Christopher J Russo
    •  & Lori A Passmore

  • Brief Communication |

    Designed β-strand peptides stabilize integral membrane proteins for biochemical and structural studies, enabling electron microscopy analysis of the dynamic conformations of the ABC transporter MsbA.

    • Houchao Tao
    • , Sung Chang Lee
    •  & Qinghai Zhang

  • News & Views |

    Two reports describing the use of direct-conversion electron detectors and algorithms that correct for beam-induced sample motion in single-particle electron cryomicroscopy demonstrate that this technique can solve structures of macromolecules at near-atomic resolution.

    • Robert M. Glaeser

  • Research Highlights |

    A milling technique affords researchers a high-resolution glimpse deep into the cell using cryoelectron tomography.

    • Allison Doerr

  • Research Highlights |

    A team of researchers applied a 'discovery single-particle profiling' experimental strategy to visualize the assembly of the ribosome via time-resolved electron microscopy.

    • Allison Doerr

  • This Month |

    Fluorescent proteins can be located in electron micrographs.

    • Monya Baker
    •  & Erik Jorgensen

  • Article |

    Methods are reported for the combination of fluorescence nanoscopy using either stimulated emission depletion microscopy (STED) or photoactivated localization microscopy (PALM) with electron microscopy, to achieve correlative imaging in which the super-resolved fluorescence signal is placed in the context of cellular ultrastructure.

    • Shigeki Watanabe
    • , Annedore Punge
    •  & Erik M Jorgensen

  • Research Highlights |

    A technique combining laser and electron pulses is used to achieve nanometer and femtosecond resolution in biological imaging.

    • Monya Baker

Browse broader subjects

Browse narrower subjects

Browse Electron microscopy across other nature.com journals