Featured
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In This Issue |
In this issue
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News |
3D cancer cells library
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Editorial |
Single-cell microbiology
Microbiologists are poised to embrace heterogeneity through the use of single-cell technologies.
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Feature |
Single-cell analysis at the threshold
A discussion of some of the challenges and promise of single-cell technology.
- Xi Chen
- , J Christopher Love
- & Sarah A Teichmann
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News & Views |
From mass cytometry to cancer prognosis
Applying social network algorithms to mass cytometry data from single cancer cells leads to more accurate predictions of patient outcomes.
- Deborah R Winter
- , Guy Ledergor
- & Ido Amit
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Article |
Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells
A seven-stage protocol enables differentiation of human embryonic stem cells into cells similar to pancreatic beta cells.
- Alireza Rezania
- , Jennifer E Bruin
- & Timothy J Kieffer
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News & Views |
Of snowflakes and natural killer cell subsets
Mass cytometric analysis reveals a remarkable and unanticipated degree of phenotypic heterogeneity among human peripheral blood natural killer cells.
- Lewis L Lanier
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Review Article |
Beyond model antigens: high-dimensional methods for the analysis of antigen-specific T cells
Davis and Newell explain how cytometry, and single-cell and sequencing methodologies can be used to characterize human T-cell responses in health and disease.
- Evan W Newell
- & Mark M Davis
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News & Views |
Cracking the code of human T-cell immunity
Combinatorial tetramer staining coupled with mass cytometry allows simultaneous detection of T cells specific for a wide array of peptide epitopes.
- Christopher J Harvey
- & Kai W Wucherpfennig
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Article |
viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia
A new tool to visualize high-dimensional single-cell data, when integrated with mass cytometry, reveals phenotypic heterogeneity of human leukemia.
- El-ad David Amir
- , Kara L Davis
- & Dana Pe'er
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Article |
Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators
Mass cytometry can measure up to 34 markers on cells, but samples cannot be multiplexed. Bodenmiller et al. use metal ion tags to facilitate multiplexed mass cytometry analysis of human blood cell samples treated with 27 kinase inhibitors and 12 stimuli across a range of dosages and time points.
- Bernd Bodenmiller
- , Eli R Zunder
- & Garry P Nolan
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Article |
SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells
Cardiomyocytes generated from human pluripotent stem cells have many potential applications in drug screening, disease modeling and cell therapy. Dubois et al. describe a cell-surface marker that allows the isolation of highly enriched populations of cardiomyocytes from differentiation cultures.
- Nicole C Dubois
- , April M Craft
- & Gordon Keller
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News & Views |
Next-generation flow cytometry
Mass cytometry dramatically enhances the dimensionality of fluorescence-based flow cytometry for phenotypic analysis of heterogeneous cell populations.
- Matthew R Janes
- & Christian Rommel
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News & Views |
Plant natural products from cultured multipotent cells
Cultured cambial meristematic cells could enable large-scale production of certain natural products.
- Susan Roberts
- & Martin Kolewe
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Letter |
Cultured cambial meristematic cells as a source of plant natural products
The culture of dedifferentiated plant cells to produce commercially important chemicals has met with limited success. Lee et al. demonstrate the potential of innately undifferentiated cells from Taxus cuspidata as an industrial source of the anticancer drug paclitaxel.
- Eun-Kyong Lee
- , Young-Woo Jin
- & Gary J Loake
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Letter |
Substrate elasticity provides mechanical signals for the expansion of hemopoietic stem and progenitor cells
Biomechanical forces may be an effective approach for controlling the behavior of stem cells in vitro. Holst et al. show that the elasticity of a tropoelastin matrix expands hematopoietic stem and progenitor cells.
- Jeff Holst
- , Sarah Watson
- & John E J Rasko
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News & Views |
Paring down signaling complexity
A new method for characterizing signaling responses to pairs of agonists predicts how cells react to higher-order combinations of external stimuli.
- Kevin A Janes
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News & Views |
Scalable pluripotent stem cell culture
Large-scale production of human embryonic stem cells will require improved culture methods.
- Larry A Couture
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News & Views |
Systematic tracking of cell fate changes
High-throughput measurements across several regulatory levels provide a comprehensive view of ES-cell differentiation.
- Jonghwan Kim
- & Stuart H Orkin
Initialization is critical for preserving global data structure in both t-SNE and UMAP
High-speed imaging in a flow