Cell biology

  • Article | | open

    The resolution limitations when using the ubiquitous algorithms that process images obtained using modern techniques are not straightforward to define. Here, the authors examine the performance of localization algorithms and use spatial statistics to provide a metric for assessing the resolution limit of such algorithms.

    • Edward A. K. Cohen
    • , Anish V. Abraham
    • , Sreevidhya Ramakrishnan
    •  & Raimund J. Ober
  • Article | | open

    Membrane elongation is fundamental to autophagy and is controlled by an ubiquitin-conjugating cascade orchestrated by ATG16L1. Here, the authors identify that the E3 ligase Gigaxonin regulates autophagosome formation by controlling ATG16L1 turnover.

    • Aurora Scrivo
    • , Patrice Codogno
    •  & Pascale Bomont
  • Article | | open

    Infection of a cell with influenza B virus (IBV) often results in cell death and the role of surviving cells in pathogenesis is unclear. Here, Dumm et al. generate a recombinant IBV that activates a host-cell reporter to permanently label infected cells, and show that surviving cells are important to preserve epithelial barrier function.

    • Rebekah E. Dumm
    • , Jessica K. Fiege
    • , Barbara M. Waring
    • , Chay T. Kuo
    • , Ryan A. Langlois
    •  & Nicholas S. Heaton
  • Article | | open

    SecB homologs can be associated with stress-responsive type II toxin–antitoxin (TA) systems and form tripartite toxin-antitoxin-chaperone systems (TAC). Here the authors provide structural insights into TACs by presenting the crystal structure of the M. tuberculosis TA-associated SecB chaperone in complex with the C-terminal ChAD (chaperone addiction) extension of the antitoxin HigA1.

    • Valérie Guillet
    • , Patricia Bordes
    • , Cécile Bon
    • , Julien Marcoux
    • , Virginie Gervais
    • , Ambre Julie Sala
    • , Suzana Dos Reis
    • , Nawel Slama
    • , Israel Mares-Mejía
    • , Anne-Marie Cirinesi
    • , Laurent Maveyraud
    • , Pierre Genevaux
    •  & Lionel Mourey
  • Article | | open

    Serine/threonine phosphatases such as PP1 associate with a large array of subunit proteins, such as ASPP (apoptosis-stimulating protein of p53) to achieve selective targeting. Here authors solved the crystal structure of the human ASPP2/PP1 complex and explain how ASPP2 can distinguish between PP1 isoforms.

    • M. Teresa Bertran
    • , Stéphane Mouilleron
    • , Yanxiang Zhou
    • , Rakhi Bajaj
    • , Federico Uliana
    • , Ganesan Senthil Kumar
    • , Audrey van Drogen
    • , Rebecca Lee
    • , Jennifer J. Banerjee
    • , Simon Hauri
    • , Nicola O’Reilly
    • , Matthias Gstaiger
    • , Rebecca Page
    • , Wolfgang Peti
    •  & Nicolas Tapon
  • Article | | open

    Immune cells produce reactive oxygen species (ROS) to eliminate pathogens, but cell-spontaneous death and ageing may also be induced. Here the authors show that, upon sensing ROS, Mst1/2 kinases modulate the activity of Nrf2 transcription factor and downstream genetic programs to protect mouse macrophages from death and ageing.

    • Ping Wang
    • , Jing Geng
    • , Jiahui Gao
    • , Hao Zhao
    • , Junhong Li
    • , Yiran Shi
    • , Bingying Yang
    • , Chen Xiao
    • , Yueyue Linghu
    • , Xiufeng Sun
    • , Xin Chen
    • , Lixin Hong
    • , Funiu Qin
    • , Xun Li
    • , Jau-Song Yu
    • , Han You
    • , Zengqiang Yuan
    • , Dawang Zhou
    • , Randy L. Johnson
    •  & Lanfen Chen
  • Article | | open

    Calcium signalling downstream of VEGF is essential for VEGF-induced angiogenesis. Here Savage et al. show that Transmembrane Protein 33 (TMEM33) is required for angiogenesis and the endothelial calcium response to VEGF, revealing a function for TMEM33 in multicellular organisms.

    • Aaron M. Savage
    • , Sathishkumar Kurusamy
    • , Yan Chen
    • , Zhen Jiang
    • , Karishma Chhabria
    • , Ryan B. MacDonald
    • , Hyejeong R. Kim
    • , Heather L. Wilson
    • , Fredericus J. M. van Eeden
    • , Angel L. Armesilla
    • , Timothy J. A. Chico
    •  & Robert N. Wilkinson
  • Article | | open

    Inter-organelle signaling coordinates adaptive responses via currently unknown mechanisms. Here, Tapia et al. show that KDEL signaling repositions lysosomes in a complex process termed ‘traffic-induced degradation response for secretion’ (TIDeRS) that connects multiple pathways and Golgi secretion.

    • Diego Tapia
    • , Tomás Jiménez
    • , Constanza Zamora
    • , Javier Espinoza
    • , Riccardo Rizzo
    • , Alexis González-Cárdenas
    • , Danitza Fuentes
    • , Sergio Hernández
    • , Viviana A. Cavieres
    • , Andrea Soza
    • , Fanny Guzmán
    • , Gloria Arriagada
    • , María Isabel Yuseff
    • , Gonzalo A. Mardones
    • , Patricia V. Burgos
    • , Alberto Luini
    • , Alfonso González
    •  & Jorge Cancino
  • Article | | open

    Homology directed repair of meiotic double-strand breaks functions via recruitment and assembly of strand-exchange proteins called recombinases. Here the authors reveal and characterize a BRCA2 interactor regulating meiotic recombinases that localizes to chromosomal axes and facilitates the repair of meiotic DSBs.

    • Jingjing Zhang
    • , Yasuhiro Fujiwara
    • , Shohei Yamamoto
    •  & Hiroki Shibuya
  • Article | | open

    BCL9 and Pygo are components of Wnt enhanceosome, which facilitates β-catenin-dependent transcription. Here, the authors show that deletion of Bcl9 and Pygo suppresses tumorigenesis and extends disease free survival in two different colorectal cancer models, suggesting a strategy for drugging β-catenin signalling in this cancer.

    • Juliusz Mieszczanek
    • , Laurens M. van Tienen
    • , Ashraf E. K. Ibrahim
    • , Douglas J. Winton
    •  & Mariann Bienz
  • Article | | open

    The antitermination factor RfaH adopts two functional states where its C-terminal domain is folded either as an α-helical hairpin or β-barrel. Here the authors employ solution state NMR measurements to show that the C-terminal domain transforms into the β-barrel only upon binding to the elongation complex and refolds back after dissociation.

    • Philipp Konrad Zuber
    • , Kristian Schweimer
    • , Paul Rösch
    • , Irina Artsimovitch
    •  & Stefan H. Knauer
  • Article | | open

    Transcription is globally repressed in early stage of embryo development, but a set of genes including pri-miR-430 and zinc finger genes is known to escape the repression. Here the authors image the very first transcriptional activities in the living zebra fish embryo, demonstrating a cell cycle-coordinated polymerase II transcription compartment.

    • Yavor Hadzhiev
    • , Haseeb K. Qureshi
    • , Lucy Wheatley
    • , Ledean Cooper
    • , Aleksandra Jasiulewicz
    • , Huy Van Nguyen
    • , Joseph W. Wragg
    • , Divyasree Poovathumkadavil
    • , Sascha Conic
    • , Sarah Bajan
    • , Attila Sik
    • , György Hutvàgner
    • , Làszlò Tora
    • , Agnieszka Gambus
    • , John S. Fossey
    •  & Ferenc Müller
  • Article | | open

    Ubiquitination may control protein stability or function. Here the authors show that an ubiquitination enzyme, Hectd3, ubiquitinates Stat3 and Malt1 to modulate their function but not degradation in T cells, and thereby promoting the differentiation of pathogenic Th17 cells and susceptibility to a mouse model of multiple sclerosis.

    • Jonathan J. Cho
    • , Zhiwei Xu
    • , Upasana Parthasarathy
    • , Theodore T. Drashansky
    • , Eric Y. Helm
    • , Ashley N. Zuniga
    • , Kyle J. Lorentsen
    • , Samira Mansouri
    • , Joshua Y. Cho
    • , Mariola J. Edelmann
    • , Duc M. Duong
    • , Torben Gehring
    • , Thomas Seeholzer
    • , Daniel Krappmann
    • , Mohammad N. Uddin
    • , Danielle Califano
    • , Rejean L. Wang
    • , Lei Jin
    • , Hongmin Li
    • , Dongwen Lv
    • , Daohong Zhou
    • , Liang Zhou
    •  & Dorina Avram
  • Article | | open

    How the chromosome passenger complex (CPC) phosphorylates the kinetochores that can be a micron away to control mitotic events is unknown. Here the authors find that the CPC directly binds microtubules near inner centromeres, which controls its ability to phosphorylate kinetochores independently of tension generated by kinetochore microtubule attachments.

    • Prasad Trivedi
    • , Anatoly V. Zaytsev
    • , Maxim Godzi
    • , Fazly I. Ataullakhanov
    • , Ekaterina L. Grishchuk
    •  & P. Todd Stukenberg
  • Article | | open

    Many 2-Cystein Peroxiredoxins (Prx) can either function as peroxidases or chaperones when exposed to stress. Here the authors present the structures of Leishmania infantum mitochondrial Prx alone and with a bound model client protein, use crosslinking to reveal interaction regions that stabilize the bound client, and provide insights into the mechanism by which Prx’s adopt chaperone activity.

    • Filipa Teixeira
    • , Eric Tse
    • , Helena Castro
    • , Karl A. T. Makepeace
    • , Ben A. Meinen
    • , Christoph H. Borchers
    • , Leslie B. Poole
    • , James C. Bardwell
    • , Ana M. Tomás
    • , Daniel R. Southworth
    •  & Ursula Jakob
  • Article | | open

    A fundamental step in membrane protein biogenesis is their integration into the lipid bilayer with a defined orientation of each transmembrane segment. Here, the authors show that mutations in connexin 32 can cause failures in membrane integration which is detected by the ER chaperone machinery.

    • João P. L. Coelho
    • , Matthias Stahl
    • , Nicolas Bloemeke
    • , Kevin Meighen-Berger
    • , Carlos Piedrafita Alvira
    • , Zai-Rong Zhang
    • , Stephan A. Sieber
    •  & Matthias J. Feige
  • Article | | open

    BMP ligands act as retrograde signalling molecules to regulate presynaptic development, and regulation of BMP receptors by endocytosis may be an important component of this signalling pathway. Here, the authors show that Abi-mediated macropinocytosis of BMP receptors in Drosophila larva and contributes to neuromuscular development.

    • Najin Kim
    • , Sungdae Kim
    • , Minyeop Nahm
    • , Danielle Kopke
    • , Joohyung Kim
    • , Eunsang Cho
    • , Min-Jung Lee
    • , Mihye Lee
    • , Seung Hyun Kim
    • , Kendal Broadie
    •  & Seungbok Lee
  • Article | | open

    PTEN is a lipid phosphatase that functions as a dose-dependent tumor suppressor through the PI3K/AKT pathway. Here the authors describe a signaling feedback mechanism where PTEN stability is regulated through transcriptional upregulation of X-linked ubiquitin-specific protease 11 (USP11) via the PI3K/FOXO pathway.

    • Mi Kyung Park
    • , Yixin Yao
    • , Weiya Xia
    • , Stephanie Rebecca Setijono
    • , Jae Hwan Kim
    • , Isabelle K. Vila
    • , Hui-Hsuan Chiu
    • , Yun Wu
    • , Enrique González Billalabeitia
    • , Min Gyu Lee
    • , Robert G. Kalb
    • , Mien-Chie Hung
    • , Pier Paolo Pandolfi
    • , Su Jung Song
    •  & Min Sup Song
  • Article | | open

    Nuclear envelopathies are a group of diseases caused by genetic mutations in essential nuclear envelope genes. Here, the authors report a nuclear envelopathy with a homozygous nonsense variant in TOR1AIP1 which leads to changes in the nuclear morphology including large nuclear-spanning channels in patients’ fibroblasts.

    • Boris Fichtman
    • , Fadia Zagairy
    • , Nitzan Biran
    • , Yiftah Barsheshet
    • , Elena Chervinsky
    • , Ziva Ben Neriah
    • , Avraham Shaag
    • , Michael Assa
    • , Orly Elpeleg
    • , Amnon Harel
    •  & Ronen Spiegel
  • Article | | open

    Zinc ions (Zn2+) are imported by Golgi-resident transporters but the function of zinc in the early secretory pathway has remained unknown. Here the authors find that Zn2+ regulates protein quality control in the early secretory pathway by demonstrating that the pH-sensitive chaperone ERp44 binds Zn2+ and solving the Zn2+-bound ERp44 structure.

    • Satoshi Watanabe
    • , Yuta Amagai
    • , Sara Sannino
    • , Tiziana Tempio
    • , Tiziana Anelli
    • , Manami Harayama
    • , Shoji Masui
    • , Ilaria Sorrentino
    • , Momo Yamada
    • , Roberto Sitia
    •  & Kenji Inaba
  • Article | | open

    The TERB1-TERB2-MAJIN complex mediates the attachment of telomeres to the nuclear envelope. Here the authors present the crystal structures of the human TERB1-TERB2 and TERB2-MAJIN subcomplexes and show that Terb2 mutations, which abolish complex formation cause aberrant homologous pairing and disordered synapsis in mouse.

    • Yan Wang
    • , Yanyan Chen
    • , Juan Chen
    • , Lijun Wang
    • , Leitong Nie
    • , Juanjuan Long
    • , Haishuang Chang
    • , Jian Wu
    • , Chenhui Huang
    •  & Ming Lei
  • Article | | open

    Ribosome-associated quality control (RQC) pathways monitor and respond to stalling of the translating ribosome. Here the authors show that the ribosome associated RQC factor Hel2/ZNF598, an E3 ubiquitin ligase, generally interacts with mRNAs in the vicinity of stop codons.

    • Marie-Luise Winz
    • , Lauri Peil
    • , Tomasz W. Turowski
    • , Juri Rappsilber
    •  & David Tollervey
  • Article | | open

    Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for in vivo imaging.

    • Jes Dreier
    • , Marco Castello
    • , Giovanna Coceano
    • , Rodrigo Cáceres
    • , Julie Plastino
    • , Giuseppe Vicidomini
    •  & Ilaria Testa
  • Perspective | | open

    Biological circadian rhythms maintain a period close to 24 h in coordination with the Earth’s fixed rotational period. Here Webb et al. discuss how external cues continuously adjust phase and period, viewing the oscillator as a dynamically-adjusted plastic system rather than tightly-coupled cogs in a mechanical clock.

    • Alex A. R. Webb
    • , Motohide Seki
    • , Akiko Satake
    •  & Camila Caldana
  • Article | | open

    The role of mesencephalic astrocyte-derived neurotrophic factor (MANF) in maintenance of protein folding homeostasis inside the ER has remained unclear. Here the authors determine the structure of the complex between MANF and the ER-localized chaperone BiP and provide evidence that MANF serves as an anti-nucleotide exchange factor for BiP.

    • Yahui Yan
    • , Claudia Rato
    • , Lukas Rohland
    • , Steffen Preissler
    •  & David Ron
  • Article | | open

    The suprachiasmatic nucleus (SCN) synchronises daily rhythms of behaviour and physiology to the light-dark cycle. Vasoactive intestinal peptide (VIP) is important for mediating SCN entrainment; however, the underlying mechanisms are incompletely understood. Here, the authors show that the effects of VIP on the SCN are mediated by ERK1/2 and DUSP4.

    • Ryan Hamnett
    • , Priya Crosby
    • , Johanna E. Chesham
    •  & Michael H. Hastings
  • Article | | open

    PGAM5 is a mitochondrial protein phosphatase whose functions include regulation of mitophagy and cell death. Here, the authors use x-ray crystallography and EM to show that PGAM5 forms dodecameric rings and filaments in solution, and find that PGAM5 rings are essential for catalysis and for a structural effect PGAM5 has on mitochondrial membranes, independently of catalytic activity.

    • Karen Ruiz
    • , Tarjani M. Thaker
    • , Christopher Agnew
    • , Lakshmi Miller-Vedam
    • , Raphael Trenker
    • , Clara Herrera
    • , Maria Ingaramo
    • , Daniel Toso
    • , Adam Frost
    •  & Natalia Jura
  • Article | | open

    High fat diets (HFD) are thought to perturb murine hematopoiesis as a result of obesity. Here the authors find that short-term HFD reduces hematopoietic stem cells (HSC), disrupts lipid rafts and TGF-β1 signalling. Injecting HFD-fed mice with recombinant TGF-β1 can rescue HSC loss.

    • François Hermetet
    • , Anne Buffière
    • , Aziza Aznague
    • , Jean-Paul Pais de Barros
    • , Jean-Noël Bastie
    • , Laurent Delva
    •  & Ronan Quéré
  • Article | | open

    Natural killer (NK) cells are functionally calibrated against self MHC during a process termed education. Here the authors show that NK cell education is associated with the accumulation of dense-core secretory lysosomes for expedited release of granzyme B and Ca2+ flux upon target recognition and NK cell activation.

    • Jodie P. Goodridge
    • , Benedikt Jacobs
    • , Michelle L. Saetersmoen
    • , Dennis Clement
    • , Quirin Hammer
    • , Trevor Clancy
    • , Ellen Skarpen
    • , Andreas Brech
    • , Johannes Landskron
    • , Christian Grimm
    • , Aline Pfefferle
    • , Leonardo Meza-Zepeda
    • , Susanne Lorenz
    • , Merete Thune Wiiger
    • , William E. Louch
    • , Eivind Heggernes Ask
    • , Lisa L. Liu
    • , Vincent Yi Sheng Oei
    • , Una Kjällquist
    • , Sten Linnarsson
    • , Sandip Patel
    • , Kjetil Taskén
    • , Harald Stenmark
    •  & Karl-Johan Malmberg
  • Article | | open

    Large protein complexes and ribonucleoprotein particles (RNPs) such as pre-ribosomes are transported from the nucleus to the cytoplasm through the nuclear pore complex (NPC). Here the authors use ultrafast freezing and electron tomography to catch snapshots of native RNPs crossing the NPC and estimate their transit time using a probabilistic model.

    • Franck Delavoie
    • , Vanessa Soldan
    • , Dana Rinaldi
    • , Jean-Yves Dauxois
    •  & Pierre-Emmanuel Gleizes
  • Article | | open

    Drebrin is an actin-binding protein known to play a role in neuronal dendritic spines but its precise regulation is unclear. Here, the authors report that DBN is activated by oxidative stress in an ATM-kinase dependent manner and increases resistance to oxidative stress in mice and in C. elegans.

    • Patricia Kreis
    • , Christian Gallrein
    • , Eugenia Rojas-Puente
    • , Till G. A. Mack
    • , Cristina Kroon
    • , Viktor Dinkel
    • , Claudia Willmes
    • , Kai Murk
    • , Susanne tom-Dieck
    • , Erin M. Schuman
    • , Janine Kirstein
    •  & Britta J. Eickholt
  • Article | | open

    Mammalian transcription factors (TFs) differ broadly in their DNA binding properties. Here authors quantify mitotic chromosome binding (MCB) of 501 TFs and suggest that MCB can be used as a proxy for non-specific TF-DNA interactions that regulate TF search for specific genomic sites.

    • Mahé Raccaud
    • , Elias T. Friman
    • , Andrea B. Alber
    • , Harsha Agarwal
    • , Cédric Deluz
    • , Timo Kuhn
    • , J. Christof M. Gebhardt
    •  & David M. Suter
  • Article | | open

    IRSp53 is a key regulator of filopodia formation and cell migration. Here, the authors elucidate a mechanism of phosphorylation-dependent inhibition of IRSp53 by 14-3-3, which impedes the interactions of IRSp53 with membranes and downstream cytoskeletal effectors.

    • David J. Kast
    •  & Roberto Dominguez
  • Article | | open

    The origin and physiological significance of lipid droplets (LDs) in the nucleus is not clear. Here authors show that nucleoplasmic LDs in hepatocytes are derived from apolipoprotein B (ApoB)-free lumenal LDs and constitute a feedback mechanism to regulate PC synthesis in accordance with ER stress.

    • Kamil Sołtysik
    • , Yuki Ohsaki
    • , Tsuyako Tatematsu
    • , Jinglei Cheng
    •  & Toyoshi Fujimoto
  • Article | | open

    The inactive X chromosome (Xi) is a model for establishment and maintenance of repressed chromatin and the function of polycomb repressive complexes. Here the authors show that Xi transiently relocates from the nuclear periphery during replication in a CIZ1-dependent manner, which plays a role in maintaining PRC-mediated repressed chromatin.

    • Emma R. Stewart
    • , Robert M. L. Turner
    • , Katherine Newling
    • , Rebeca Ridings-Figueroa
    • , Victoria Scott
    • , Peter D. Ashton
    • , Justin F. X. Ainscough
    •  & Dawn Coverley
  • Article | | open

    Designing interfaces that can induce protein-protein interactions is a challenging problem. Here the authors show that a five amino acid sequence known to mediate domain swapping in cystatins can drive oligomerization when grafted onto functionally and structurally unrelated host proteins, providing a simple approach to the design of protein assemblies.

    • Neha Nandwani
    • , Parag Surana
    • , Hitendra Negi
    • , Nahren M. Mascarenhas
    • , Jayant B. Udgaonkar
    • , Ranabir Das
    •  & Shachi Gosavi
  • Article | | open

    Studying the condensation of proteins into membraneless organelles in live cells is highly challenging. Here the authors develop a fluorescence lifetime imaging approach to monitor the condensation of proteins in nuclear organelles and report coordinated and cyclic changes in several nuclear organelles.

    • Artem Pliss
    • , Svitlana M. Levchenko
    • , Lixin Liu
    • , Xiao Peng
    • , Tymish Y. Ohulchanskyy
    • , Indrajit Roy
    • , Andrey N. Kuzmin
    • , Junle Qu
    •  & Paras N. Prasad
  • Article | | open

    PACSIN proteins are known to mediate membrane tubulation. Here the authors show that PACSIN - positive membranous tubules extend from the ciliary pocket membrane and during ciliogenesis from the ciliary vesicle to the plasma membrane, called extracellular membrane channels (EMCs).

    • Christine Insinna
    • , Quanlong Lu
    • , Isabella Teixeira
    • , Adam Harned
    • , Elizabeth M. Semler
    • , Jim Stauffer
    • , Valentin Magidson
    • , Ajit Tiwari
    • , Anne K. Kenworthy
    • , Kedar Narayan
    •  & Christopher J. Westlake
  • Article | | open

    Insulin resistance is associated with development of type 2 diabetes. Here the authors show that TAZ interacts with c-Jun and Tead4, inducing expression of the insulin receptor substrate 1 (IRS1) leading to increased glucose uptake in muscle, and that its activity is counteracted by statin administration.

    • Jun-Ha Hwang
    • , A Rum Kim
    • , Kyung Min Kim
    • , Jung Il Park
    • , Ho Taek Oh
    • , Sung A Moon
    • , Mi Ran Byun
    • , Hana Jeong
    • , Hyo Kyung Kim
    • , Michael B. Yaffe
    • , Eun Sook Hwang
    •  & Jeong-Ho Hong
  • Article | | open

    ER stress and UPR are implicated in various cancers. Here, the authors show that one of the canonical UPR pathways, IRE1α-XBP1 regulates c-MYC signaling to promote prostate tumorigenesis, and pharmacological inhibition of IRE1α with MKC8866 inhibits prostate cancer growth and synergizes with clinically used prostate cancer drugs.

    • Xia Sheng
    • , Hatice Zeynep Nenseth
    • , Su Qu
    • , Omer F. Kuzu
    • , Turid Frahnow
    • , Lukas Simon
    • , Stephanie Greene
    • , Qingping Zeng
    • , Ladan Fazli
    • , Paul S. Rennie
    • , Ian G. Mills
    • , Håvard Danielsen
    • , Fabian Theis
    • , John B. Patterson
    • , Yang Jin
    •  & Fahri Saatcioglu
  • Article | | open

    Hippo signaling leads to the phosphorylation of the key transcriptional effector, Yap/Yki, although how Yap/Yki stability is regulated has remained unclear. Here, Sun et al. identify HAUSP/Usp7 as a conserved and clinically relevant regulator of the Hippo pathway that increases Yap/Yki stability.

    • Xiaohan Sun
    • , Yan Ding
    • , Meixiao Zhan
    • , Yan Li
    • , Dongqing Gao
    • , Guiping Wang
    • , Yang Gao
    • , Yong Li
    • , Shian Wu
    • , Ligong Lu
    • , Qingxin Liu
    •  & Zizhang Zhou
  • Article | | open

    Glycogen Synthase Kinase 3 (GSK3) has conserved functions across species but its role in the kidney is unclear. Here, the authors show that in the kidney podocyte cell of mice and related nephrocyte in Drosophila that GSK3 is a master regulator of function both during development and in maturity.

    • J. A. Hurcombe
    • , P. Hartley
    • , A. C. Lay
    • , L. Ni
    • , J. J. Bedford
    • , J. P. Leader
    • , S. Singh
    • , A. Murphy
    • , C. L. Scudamore
    • , E. Marquez
    • , A. F. Barrington
    • , V. Pinto
    • , M. Marchetti
    • , L.-F. Wong
    • , J. Uney
    • , M. A. Saleem
    • , P. W. Mathieson
    • , S. Patel
    • , R. J. Walker
    • , J. R. Woodgett
    • , S. E. Quaggin
    • , G. I. Welsh
    •  & R. J. M. Coward
  • Article | | open

    The connection between DNA replication timing and changes that occur to the epigenome in cancer are still poorly understood. Here, the authors perform Repli-Seq and integrated epigenome analyses and find that genomic regions that undergo long-range epigenetic deregulation in prostate cancer also show concordant differences in replication timing.

    • Qian Du
    • , Saul A. Bert
    • , Nicola J. Armstrong
    • , C. Elizabeth Caldon
    • , Jenny Z. Song
    • , Shalima S. Nair
    • , Cathryn M. Gould
    • , Phuc-Loi Luu
    • , Timothy Peters
    • , Amanda Khoury
    • , Wenjia Qu
    • , Elena Zotenko
    • , Clare Stirzaker
    •  & Susan J. Clark
  • Article | | open

    Selective autophagy receptors are thought to selectively recruit Atg8 positive membranes to cargo via their LIR motif. Here, the authors show the LIR motifs in OPTN and NDP52 are dispensable for selectivity, functioning instead to recruit additional receptors and amplify mitophagy.

    • Benjamin Scott Padman
    • , Thanh Ngoc Nguyen
    • , Louise Uoselis
    • , Marvin Skulsuppaisarn
    • , Lan K. Nguyen
    •  & Michael Lazarou
  • Article | | open

    Cortical microtubules of Toxoplasma gondii are exceptionally stable, but it isn’t known how they are anchored along membranes. Here, Harding et al. show that GAPM proteins localize to the inner membrane complex and are essential for maintaining the structural stability of parasites.

    • Clare R. Harding
    • , Matthew Gow
    • , Joon Ho Kang
    • , Emily Shortt
    • , Scott R. Manalis
    • , Markus Meissner
    •  & Sebastian Lourido
  • Article | | open

    The circadian clock can affect pathogen replication, but underlying molecular mechanisms are unclear. Here the authors demonstrate that the circadian components BMAL1 and REV-ERBα affect entry of hepatitis C virus (HCV) into hepatocytes and genome replication of HCV and related flaviviruses dengue and zika.

    • Xiaodong Zhuang
    • , Andrea Magri
    • , Michelle Hill
    • , Alvina G. Lai
    • , Abhinav Kumar
    • , Srinivasa Bhargav Rambhatla
    • , Claire L. Donald
    • , Andrea F. Lopez-Clavijo
    • , Simon Rudge
    • , Katherine Pinnick
    • , Wai Hoong Chang
    • , Peter A. C. Wing
    • , Ryan Brown
    • , Ximing Qin
    • , Peter Simmonds
    • , Thomas F. Baumert
    • , David Ray
    • , Andrew Loudon
    • , Peter Balfe
    • , Michael Wakelam
    • , Sam Butterworth
    • , Alain Kohl
    • , Catherine L. Jopling
    • , Nicole Zitzmann
    •  & Jane A. McKeating
  • Article | | open

    The transcription factor Foxp3 and Stat5 modulate lineage stability and function of regulatory T (Treg) cells to promote immune homeostasis. Here the authors show that O-GlcNAcylation of Foxp3 and Stat5, mediated by O-GlcNAc transferase (OGT), is essential for Treg-mediate immune balance, with Treg-specific deficiency of OGT leading to severe autoimmunity.

    • Bing Liu
    • , Oscar C. Salgado
    • , Sangya Singh
    • , Keli L. Hippen
    • , Jason C. Maynard
    • , Alma L. Burlingame
    • , Lauren E. Ball
    • , Bruce R. Blazar
    • , Michael A. Farrar
    • , Kristin A. Hogquist
    •  & Hai-Bin Ruan
  • Article | | open

    Cells divide within a given size range and can scale across differing cell sizes but mechanisms and function remain unclear. Here the authors show, despite the current dogma of fission yeast maintaining constant width, some fission yeast can scale their width and length, impacting the positioning of the cell division site.

    • Ying Gu
    •  & Snezhana Oliferenko
  • Article | | open

    The architecture of functional TNTs is still under debate. Here, the authors combine correlative FIB-SEM, light- and cryo-electron microscopy approaches to elucidate the structure of TNTs in neuronal cells, showing that they form structures that are distinct form other membrane protrusions.

    • Anna Sartori-Rupp
    • , Diégo Cordero Cervantes
    • , Anna Pepe
    • , Karine Gousset
    • , Elise Delage
    • , Simon Corroyer-Dulmont
    • , Christine Schmitt
    • , Jacomina Krijnse-Locker
    •  & Chiara Zurzolo