Single-molecule localization microscopy

SMLM describes a family of imaging techniques with increased spatial resolution, able to find individual fluorescent molecules. 

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  • This Primer explains the central concepts of single-molecule localization microscopy (SMLM) before discussing experimental considerations regarding fluorophores, optics and data acquisition, processing and analysis. The Primer further describes recent high-impact discoveries made by SMLM techniques and concludes by discussing emerging methodologies.

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