Volume 6 Issue 6, June 2011

A confocal image of FGF-2induced corneal new blood vessels (CD31, red) and lymphatic vessels (LYVE-1, green). Corneal lymphatic networks comprise large-diameter vessels with blunt-ended vascular sprouts, whereas blood vessel networks are composed of smaller microvessels at higher densities relative to lymphatics.

Protocol

Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection

Emily Dick
Elena Matsa
Chris Denning
Protocol 05 May 2011
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Initiation, growth and cryopreservation of plant cell suspension cultures

Natali R Mustafa
Ward de Winter
Robert Verpoorte
Protocol 05 May 2011
Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst

Jianguo Xia
David S Wishart
Protocol 05 May 2011
Synthesis of all-hydrocarbon stapled α-helical peptides by ring-closing olefin metathesis

Young-Woo Kim
Tom N Grossmann
Gregory L Verdine
Protocol 12 May 2011
Rapid DNA detection by beacon-assisted detection amplification

Ashley R Connolly
Matt Trau
Protocol 12 May 2011
Flexizymes for genetic code reprogramming

Yuki Goto
Takayuki Katoh
Hiroaki Suga
Protocol 12 May 2011
Generation of phospholipid vesicle-nanotube networks and transport of molecules therein

Aldo Jesorka
Natalia Stepanyants
Owe Orwar
Protocol 19 May 2011
A simple, versatile and efficient method to genetically modify human monocyte-derived dendritic cells with HIV-1–derived lentiviral vectors

Grégory Berger
Stéphanie Durand
Andrea Cimarelli
Protocol 19 May 2011
Mouse corneal lymphangiogenesis model

Renhai Cao
Sharon Lim
Yihai Cao
Protocol 19 May 2011
Generating gene knockout rats by homologous recombination in embryonic stem cells

Chang Tong
Guanyi Huang
Qi-Long Ying
Protocol 26 May 2011
Imaging three-dimensional tissue architectures by focused ion beam scanning electron microscopy

Andrew J Bushby
Kenneth M Y P'ng
Andrew J Quantock
Protocol 26 May 2011
Monitoring protein expression in whole-cell extracts by targeted label- and standard-free LC-MS/MS

Katharina Bluemlein
Markus Ralser
Protocol 26 May 2011
Genome-scale analysis of replication timing: from bench to bioinformatics

Tyrone Ryba
Dana Battaglia
David M Gilbert
Protocol 02 Jun 2011
In vivo solid-phase microextraction for monitoring intravenous concentrations of drugs and metabolites

Heather L Lord
Xu Zhang
Janusz Pawliszyn
Protocol 02 Jun 2011
A time-of–drug addition approach to target identification of antiviral compounds

Dirk Daelemans
Rudi Pauwels
Christophe Pannecouque
Protocol 02 Jun 2011

Volume 6 Issue 6, June 2011

Protocol

Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection

Initiation, growth and cryopreservation of plant cell suspension cultures

Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst

Synthesis of all-hydrocarbon stapled α-helical peptides by ring-closing olefin metathesis

Rapid DNA detection by beacon-assisted detection amplification

Flexizymes for genetic code reprogramming

Generation of phospholipid vesicle-nanotube networks and transport of molecules therein

A simple, versatile and efficient method to genetically modify human monocyte-derived dendritic cells with HIV-1–derived lentiviral vectors

Mouse corneal lymphangiogenesis model

Generating gene knockout rats by homologous recombination in embryonic stem cells

Imaging three-dimensional tissue architectures by focused ion beam scanning electron microscopy

Monitoring protein expression in whole-cell extracts by targeted label- and standard-free LC-MS/MS

Genome-scale analysis of replication timing: from bench to bioinformatics

In vivo solid-phase microextraction for monitoring intravenous concentrations of drugs and metabolites

A time-of–drug addition approach to target identification of antiviral compounds

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