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Triple fluorescence co-detection of sterols (red), the cytokinesis-specific KNOLLE syntaxin (cyan) and an enhanced green fluorescent protein (EGFP) fusion of the small GTPase ARF1 (ARF1-EGFP) in dividing root epidermal cells of Arabidopsis thaliana. The protocol visualizes fluorescent filipin-sterol complexes in plants expressing ARF1-EGFP by confocal laser scanning microscopy. Cover design by Jamel Wooten.