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A tiling confocal microscopic image of brain coronal section shows blockage of downstream cerebral microvascular perfusion supplied by the middle cerebral artery (MCA) in a rat subjected to embolic MCA occlusion. Taken from the protocol by Zhang et al. doi: 10.1038/nprot.2015.036. Cover design by Jamel Wooten.
In this model, the middle cerebral artery (MCA) is occluded with a fibrin-rich allogeneic clot, producing an embolic model of MCA occlusion in the rat that mimics the key components of neurovascular damage observed in human ischemic stroke.
Hydrogenation of functionalized substrates often relies on expensive noble metal—based catalysts. Jagadeesh et al. detail the hydrogenation of nitroarenes and the reductive amination of aldehydes with nitroarenes performed with an iron-based catalyst.
Optically active, chiral quantum dots can be prepared using chiral ligands. These nanoparticles have potential applications in photocatalysis and biological imaging, as well as in assays and sensors in asymmetric synthesis and enantioseparation.
This protocol describes live imaging of axonal transport in Drosophila pupal brains. It complements previous techniques for imaging larval neurons by enabling the study of extensive changes occurring during metamorphosis.
This protocol describes an efficient double-click reaction between dialkynyl linkers and diazido peptides for generating peptides that are stabilized in an α-helical conformation. The method enables modular control over the staple linkage itself.
Through screening libraries of polymer nanoparticles (NPs) containing various comonomers, it is possible to develop NPs with high affinity for target biomacromolecules. This protocol describes the preparation and characterization of melittin-binding NPs.
4sUDRB-seq is a genome-wide method for measuring the transcription rate of actively transcribed genes. It can provide information on the speed of elongation and on the rate at which RNA polymerase II makes the transition into active elongation.
Cheow et al. provide a protocol for SCRAM, a method for determining DNA methylation status at defined target sites in single cells. It is reliable, low in cost and relatively fast, making it a good option when full genome coverage is not required.
Carrier mobility is the most important parameter for assessing charge carrier transfer. This protocol describes a tool for predicting the charge carrier mobilities of π-stacked systems such as organic semiconductors and the DNA double helix.