Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
The GelMap workflow adds a fluorescent grid into samples before expansion, allowing for precise determination of expansion factor and subsequent deformation correction in ExM. GelMap works with diverse samples and expansion methods.
Montage parallel array cryo-tomography adopts principles of montage tomography via regular array beam-image-shift montage acquisition and is robust for imaging large fields of view while retaining high-resolution structural information in cryo-electron tomography.
The Clivias are a series of small, monomeric fluorescent RNAs that emit with a large Stokes shift in the orange–red. They enable multiplexed RNA imaging in live cells and BRET-based detection of protein–RNA interactions in mice.
Statistically unbiased prediction utilizing spatiotemporal information in imaging data (SUPPORT) is a self-supervised deep learning approach to accurately denoise voltage and calcium imaging data while preserving true dynamic signals.
This work introduces a wet lab and computational pipeline, Napu, for small variant calling and de novo assembly of Nanopore sequencing data, which leads to comparable performances to short-read sequencing and allows for large-scale long-read sequencing projects.
This paper presents an improved approach for mapping single-cell RNA-seq reads with optimized transcriptomic references, which markedly recovers previously missing gene expression data.
This Registered Report describes an extensive comparison of 22 near-infrared fluorescent proteins in vitro, in cultured mammalian cells, and in model animals, clarifying top performers in diverse biological settings.
SABER-IMC combines DNA-based signal amplification by exchange reaction (SABER) with imaging mass cytometry (IMC) to enable simultaneous and highly multiplexed marker detection, even of low-abundance markers not detectable with IMC alone.
Multielement Z-tag X-ray fluorescence (MEZ-XRF) offers a new avenue for nondestructive and highly multiplexed tissue imaging and operates from the nanometer to whole-tissue scale, unlocking new biological observations.
scNanoHi-C combines Nanopore long-read sequencing with a proximity-ligation-based Hi-C protocol to profile high-order genome structures in individual cells, enabling the capture of multiway interactions among enhancers and promoters.
CATCHFIRE (chemically assisted tethering of chimera by fluorogenic-induced recognition) tools are small tags that can chemically dimerize with turn-on fluorescence, enabling simultaneous control and visualization of protein proximity.
This article describes a time-resolved cryo-plunger that combines a droplet-based microfluidic mixer with a laser-induced generator of microjets that allows rapid reaction initiation and plunge-freezing of cryo-EM grids. A time resolution of 5 ms was achieved using this approach.
Compared to other sequences, extra-long tandem repeats, such as centromeres and immunoglobulin loci, are more difficult to align. This study presents UniAligner, a computational method for efficiently and accurately aligning extra-long tandem repeats, facilitating analysis of their variation and evolution.
contrastiveVI separates single-cell gene expression variations uniquely related to treatment conditions from those shared with control conditions using a probabilistic latent variable model.