Volume 31 Issue 3, March 2013

Volume 31 Issue 3

Artistic rendition of DNA cleavage directed by the Cas9 endonuclease complexed with a synthetic single guide RNA. Three groups demonstrate site-specific genome modification in zebrafish embryos (p 227), human cells (p 230) and bacteria (p 233). Credit: Stephen Dixon

Editorial

  • Editorial |

    Increasing public and private sector cooperation to support long-term, longitudinal population studies is critical to advance translational research on complex diseases.

News

Bioentrepreneur

Opinion and Comment

Feature

News and Views

  • News & Views |

    The Cas9 endonuclease is reprogrammed by RNAs for site-specific modification of eukaryotic and microbial genomes.

    • Claudio Mussolino
    •  & Toni Cathomen
  • News & Views |

    The key challenge in the field of molecular and cell-based therapies for heart regeneration is controlled delivery.

    • Boon-Seng Soh
    • , Hao Wu
    •  & Kenneth R Chien
  • News & Views |

    A new method for amplifying DNA allows more-uniform sequencing coverage of the genome of a single cell.

    • Roger S Lasken

Computational Biology

    Analysis

  • Analysis |

    The MuTect algorithm for calling somatic point mutations enables subclonal analysis of the whole-genome or whole-exome sequencing data being generated in large-scale cancer genomics projects.

    • Kristian Cibulskis
    • , Michael S Lawrence
    • , Scott L Carter
    • , Andrey Sivachenko
    • , David Jaffe
    • , Carrie Sougnez
    • , Stacey Gabriel
    • , Matthew Meyerson
    • , Eric S Lander
    •  & Gad Getz

Research

    Perspective

  • Brief Communications

  • Articles

  • Article |

    A CRISPR-Cas system is harnessed to introduce template-driven mutations in S. pneumoniae and E. coli at high efficiency without requiring selectable markers.

    • Wenyan Jiang
    • , David Bikard
    • , David Cox
    • , Feng Zhang
    •  & Luciano A Marraffini
  • Article | | open

    A draft sequence of the staple crop kabuli chickpea, together with resequencing and analysis of 90 additional lines from 10 countries, provides a resource for breeders.

    • Rajeev K Varshney
    • , Chi Song
    • , Rachit K Saxena
    • , Sarwar Azam
    • , Sheng Yu
    • , Andrew G Sharpe
    • , Steven Cannon
    • , Jongmin Baek
    • , Benjamin D Rosen
    • , Bunyamin Tar'an
    • , Teresa Millan
    • , Xudong Zhang
    • , Larissa D Ramsay
    • , Aiko Iwata
    • , Ying Wang
    • , William Nelson
    • , Andrew D Farmer
    • , Pooran M Gaur
    • , Carol Soderlund
    • , R Varma Penmetsa
    • , Chunyan Xu
    • , Arvind K Bharti
    • , Weiming He
    • , Peter Winter
    • , Shancen Zhao
    • , James K Hane
    • , Noelia Carrasquilla-Garcia
    • , Janet A Condie
    • , Hari D Upadhyaya
    • , Ming-Cheng Luo
    • , Mahendar Thudi
    • , C L L Gowda
    • , Narendra P Singh
    • , Judith Lichtenzveig
    • , Krishna K Gali
    • , Josefa Rubio
    • , N Nadarajan
    • , Jaroslav Dolezel
    • , Kailash C Bansal
    • , Xun Xu
    • , David Edwards
    • , Gengyun Zhang
    • , Guenter Kahl
    • , Juan Gil
    • , Karam B Singh
    • , Swapan K Datta
    • , Scott A Jackson
    • , Jun Wang
    •  & Douglas R Cook
  • Letter

  • Resources

  • Resource |

    A collection of TALENs targeted to 18,740 human protein-coding genes will facilitate genetic engineering of human cells.

    • Yongsub Kim
    • , Jiyeon Kweon
    • , Annie Kim
    • , Jae Kyung Chon
    • , Ji Yeon Yoo
    • , Hye Joo Kim
    • , Sojung Kim
    • , Choongil Lee
    • , Euihwan Jeong
    • , Eugene Chung
    • , Doyoung Kim
    • , Mi Seon Lee
    • , Eun Mi Go
    • , Hye Jung Song
    • , Hwangbeom Kim
    • , Namjin Cho
    • , Duhee Bang
    • , Seokjoong Kim
    •  & Jin-Soo Kim

Careers and Recruitment