George F Murphy

Welcome to Laboratory Investigation

Advancing the understanding of human and experimental disease

Latest Issue

Laboratory Investigation is a Transformative Journal; authors can publish using the traditional publishing route OR Open Access.
Our Open Access option complies with funder and institutional requirements.

Latest Research

  • Article |

    Using approaches involving chimeric mice and macrophage-adipocyte cocultures, the authors demonstrate that PFKFB3 disruption only in hematopoietic cells exacerbates the severity of diet-induced adipose tissue inflammation and systemic insulin resistance. Mechanistically, macrophage factors generated in response to PFKFB3 disruption act to enhance adipocyte proinflammatory responses and impair adipocyte insulin signaling.

    • Bilian Zhu
    • , Xin Guo
    • , Hang Xu
    • , Boxiong Jiang
    • , Honggui Li
    • , Yina Wang
    • , Qiongli Yin
    • , Tianhao Zhou
    • , James J. Cai
    • , Shannon Glaser
    • , Fanyin Meng
    • , Heather Francis
    • , Gianfranco Alpini
    •  & Chaodong Wu
  • Review Article |

    Data processing and learning has become a spearhead for the advancement of medicine. Computational pathology is burgeoning subspecialty that promises a better-integrated solution to whole-slide images, multi-omics data and clinical informatics as innovative approach for patient care. This review describes clinical perspectives and discusses the statistical methods, clinical applications, potential obstacles, and future directions of computational pathology.

    • Miao Cui
    •  & David Y. Zhang
  • Article |

    Recent studies highlight a prominent role of human lung pericytes in lung diseases. However, human lung pericytes usually stop growth within ten passages. This study successfully established an immortalized human lung pericyte cell line, which exhibited sustained proliferation, retained pericyte characteristics and function, is a promising tool for in vitro pericyte studies.

    • Pengfei Li
    • , Yan Wu
    • , Andrew J. Goodwin
    • , Perry V. Halushka
    • , Carole L. Wilson
    • , Lynn M. Schnapp
    •  & Hongkuan Fan
  • Article |

    In this study, the authors reveal that the hypoxic microenvironment stimulates glioma to generate miR-1246- and miR-10b-5p-rich exosomes, which are delivered to normoxic glioma cells to promote their migration and invasion by targeting FRK and TFAP2A respectively. Treatment targeting the exosomes and microRNAs may impair the motility of gliomas, providing a novel direction for the development of antitumor therapy.

    • Mingyu Qian
    • , Zihang Chen
    • , Xiaofan Guo
    • , Shaobo Wang
    • , Zongpu Zhang
    • , Wei Qiu
    • , Yanhua Qi
    • , Shouji Zhang
    • , Jianye Xu
    • , Rongrong Zhao
    • , Hao Xue
    •  & Gang Li
  • Technical Report |

    Tissue cytometry is a promising new microscopy technique that can be used to enumerate and characterize each cell in a tissue. Here the authors describe a complete and accessible pipeline, including methods of sample preparation, microscopy, image analysis, and data analysis for large-scale three-dimensional tissue cytometry of human kidney tissues.

    • Michael J. Ferkowicz
    • , Seth Winfree
    • , Angela R. Sabo
    • , Malgorzata M. Kamocka
    • , Suraj Khochare
    • , Daria Barwinska
    • , Michael T. Eadon
    • , Ying-Hua Cheng
    • , Carrie L. Phillips
    • , Timothy A. Sutton
    • , Katherine J. Kelly
    • , Pierre C. Dagher
    • , Tarek M. El-Achkar
    • , Kenneth W. Dunn
    • , Richard Knight
    • , Stewart Lecker
    • , Isaac Stillman
    • , Gearoid Mcmahon
    • , Sus Waikar
    • , Astrid Weins
    • , Nir Hacohen
    • , Paul Hoover
    • , Mark Aulisio
    • , Leslie Cooperman
    • , Leal Herlitz
    • , John O’toole
    • , Emilio Poggio
    • , John Sedor
    • , Paul Appelbaum
    • , Jonathan Barasch
    • , Andrew Bomback
    • , Vivette D’agati
    • , Krzysztof Kiryluk
    • , Karla Mehl
    • , Ning (Sunny) Shang
    • , Chenhua Weng
    • , Laura Barisoni
    • , Theodore Alexandrov
    • , Tarek Ashkar
    • , Daria Barwinska
    • , Pierre Dagher
    • , Kenneth Dunn
    • , Michael Eadon
    • , Michael Ferkowicz
    • , Katherine Kelly
    • , Timothy Sutton
    • , Seth Winfree
    • , Steven Menez
    • , Chirag Parikh
    • , Avi Rosenberg
    • , Pam Villalobos
    • , Alison Slack
    • , Sylvia Rosas
    • , Mark Williams
    • , Evren Azeloglu
    • , Cijang (John) He
    • , Ravi Iyengar
    • , Samir Parikh
    • , Chris Anderton
    • , Ljiljana Pasa-Tolic
    • , Dusan Velickovic
    • , George (Holt) Oliver
    • , Joseph Ardayfio
    • , Jack Bebiak
    • , Keith Brown
    • , Taneisha Campbell
    • , Catherine Campbell
    • , Lynda Hayashi
    • , Nichole Jefferson
    • , Robert Koewler
    • , Glenda Roberts
    • , John Saul
    • , Anna Shpigel
    • , Edith Christine Stutzke
    • , Lorenda Wright
    • , Leslie Miegs
    • , Roy Pinkeney
    • , Rachel Sealfon
    • , Olga Troyanskaya
    • , Katherine Tuttle
    • , Yury Goltsev
    • , Blue Lake
    • , Kun Zhang
    • , Dejan Dobi
    • , Maria Joanes
    • , Zoltan Laszik
    • , Garry Nolan
    • , Andrew Schroeder
    • , Ulysses Balis
    • , Oliver He
    • , Jeffrey Hodgin
    • , Matthias Kretzler
    • , Laura Mariani
    • , Rajasree Menon
    • , Edgar Otto
    • , Jennifer Schaub
    • , Becky Steck
    • , Michele Elder
    • , Daniel Hall
    • , John Kellum
    • , Mary Kruth
    • , Raghav Murugan
    • , Paul Palevsky
    • , Parmjeet Randhawa
    • , Matthew Rosengart
    • , Sunny Sims-Lucas
    • , Mary Stefanick
    • , Stacy Stull
    • , Mitchell Tublin
    • , Charles Alpers
    • , Ian De Boer
    • , Malia Fullerton
    • , Jonathan Himmelfarb
    • , Robyn Mcclelland
    • , Sean Mooney
    • , Stuart Shankland
    • , Kayleen Williams
    • , Kristina Blank
    • , Ashveena Dighe
    • , Jonas Carson
    • , Frederick Dowd
    • , Zach Drager
    • , Kumar Sharma
    • , Guanshi Zhang
    • , Asra Kermani
    • , Simon Lee
    • , Christopher Lu
    • , Tyler Miller
    • , Orson Moe
    • , Harold Park
    • , Kamalanathan Sambandam
    • , Francisco Sanchez
    • , Jose Torrealba
    • , Toto Robert
    • , Miguel Vazquez
    • , Nancy Wang
    • , Joe Gaut
    • , Sanjay Jain
    • , Anitha Vijayan
    • , Randy Luciano
    • , Dennis Moledina
    • , Ugwuowo Ugochukwu
    •  & Francis Perry Wilson
  • Article |

    The abnormal differentiation of Th17 cells is a vital promoter of immune thrombocytopenia (ITP) progression. In this study, the authors found that miR-199a-5p is downregulated during ITP. Supplementation of extracellular vesicles (EVs) derived from miR-199a-5p-modified ADSCs markedly repress Th17 differentiation by transferring miR-199a-5p to CD4+T cells, thus ameliorating experimental ITP.

    • Jianqin Li
    • , Yanlin Xia
    • , Xiaoru Fan
    • , Xiaofang Wu
    • , Feiyun Yang
    • , Shaoyan Hu
    •  & Zhaoyue Wang

News & Comment

Supplement

United States & Canadian Academy of Pathology 109th Annual Meeting Abstracts: Eyes on You

“Security Retina Scanner on Blue Human Eye” Geebshot/iStock

United States & Canadian Academy of Pathology 109th Annual Meeting Abstracts: Eyes on You

The largest gathering of physician-pathologists in the world
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