Multicolor flow cytometry

Flow cytometry is a powerful laser-based technology commonly used for cell counting, cell sorting and biomarker detection. Modern flow cytometers have come a long way since the first impedance-based flow cytometry device was patented in 1953. Due to developments in hardware, software and new fluorescent reagents it has now become possible to measure multiple parameters of individual cells with enhanced efficiency and quality and, notably, to generate more data with a smaller sample size than would be required for serial analysis. How have the advances of modern multicolor flow cytometry driven research forward, especially in the field of immunology?

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Multicolor flow cytometry


Multicolor flow cytometry

Jamie D K Wilson



Early specification of CD8+ T lymphocyte fates during adaptive immunity revealed by single-cell gene-expression analyses

Janilyn Arsenio, Boyko Kakaradov, Patrick J Metz, Stephanie H Kim, Gene W Yeo and John T Chang

Nature Immunology 15: 365-372; Published online, 02 March 2014; doi:10.1038/ni.2842

Origin of monocytes and macrophages in a committed progenitor

Jan Hettinger, David M Richards, Jenny Hansson, Melanie M Barra, Ann-Cathrin Joschko, Jeroen Krijgsveld and Markus Feuerer

Nature Immunology 14: 821-830; Published online, 30 June 2013; doi:10.1038/ni.2638

TCF-1 and LEF-1 act upstream of Th-POK to promote the CD4+ T cell fate and interact with Runx3 to silence Cd4 in CD8+ T cells

Farrah C Steinke, Shuyang Yu, Xinyuan Zhou, Bing He, Wenjing Yang, Bo Zhou, Hiroshi Kawamoto, Jun Zhu, Kai Tan and Hai-Hui Xue

Nature Immunology 15: 646-656; Published online, 18 May 2014; doi:10.1038/ni.2897

The TCR's sensitivity to self peptide–MHC dictates the ability of naive CD8+ T cells to respond to foreign antigens

Ross B Fulton, Sara E Hamilton, Yan Xing, J Adam Best, Ananda W Goldrath, Kristin A Hogquist and Stephen C Jameson

Nature Immunology 16: 107-117; Published online, 24 November 2014; doi:10.1038/ni.3043


Brief Communications

Transcriptional profiling of cells sorted by RNA abundance

Sandy Klemm, Stefan Semrau, Kay Wiebrands, Dylan Mooijman, Dina A Faddah, Rudolf Jaenisch and Alexander van Oudenaarden

Nature Methods 11: 549-551; Published online, 30 March 2014; doi:10.1038/nmeth.2910

Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan, Saskia Polling, Cheryl P Z Chia, Ivan H W Ng, Angelique R Ormsby, Nathan P Croft, Anthony W Purcell, Marie A Bogoyevitch, Dominic C H Ng, Paul A Gleeson and Danny M Hatters

Nature Methods 9: 467-470; Published online, 18 March 2012; doi:10.1038/nmeth.1930

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