Abstract
Substantial amounts of nonendocrine cells are implanted as part of human islet grafts, and a possible influence of nonendocrine cells on clinical islet transplantation outcome has been postulated. There are currently no product release criteria specific for nonendocrine cells due to lack of available methods. The aims of this study were to develop a method for the evaluation of pancreatic ductal cells (PDCs) for clinical islet transplantation and to characterize them regarding phenotype, viability, and function. We assessed 161 human islet preparations using laser scanning cytometry (LSC/iCys) for phenotypic analysis of nonendocrine cells and flow cytometry (FACS) for PDC viability. PDC and β-cells obtained from different density fractions during the islet cell purification were compared in terms of viability. Furthermore, we examined PDC ability to produce proinflammatory cytokines/chemokines, vascular endothelial growth factor (VEGF) and tissue factor (TF) relevant to islet graft outcome. Phenotypic analysis by LSC/iCys indicated that single staining for CK19 or CA19-9 was not enough for identifying PDCs, and that double staining for amylase and CK19 or CA19-9 allowed for quantitative evaluation of acinar cells and PDC content in human islet preparation. PDC showed a significantly higher viability than β-cells (PDC vs β-cell: 75.5±13.9 and 62.7±18.7%; P<0.0001). Although β-cell viability was independent of its density, that of PDCs was higher as the density from which they were recovered increased. There was no correlation between PDCs and β-cell viability (R2=0.0078). PDCs sorted from high-density fractions produced significantly higher amounts of proinflammatory mediators and VEGF, but not TF. We conclude that PDCs isolated from different fractions had different viability and functions. The precise characterization and assessment of these cells in addition to β-cells in human islet cell products may be of assistance in understanding their contribution to islet engraftment and in developing strategies to enhance islet graft function.
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Main
Clinical islet transplantation results in insulin independence and normalization of glycemic control in patients with type 1 diabetes mellitus.1, 2, 3, 4 Current limitations of this approach include survival of transplanted islets, which eventually leads to reintroduction of exogenous insulin.4, 5, 6 Reduction or impairment of islet regeneration might potentially contribute to the observed loss of function over time in islet graft recipients. This phenomenon may be associated with adverse effects of immunosuppressive drugs, the unsuitable implantation site (liver) for islet regeneration, and/or loss of β-cell precursors in the transplanted islet preparations.7, 8, 9
Pancreatic ductal cells (PDCs) have been shown to differentiate into insulin-producing cells in rodents and humans.10, 11, 12, 13 PDC can improve islet survival14 and engraftment in rodents, which was associated with their ability of producing insulin growth factor II (IGF-II).15 Moreover, Korbutt et al have reported a positive correlation between the proportion of transplanted PDC and the long-term metabolic success of clinical islet transplantation.16 Additionally, Pipeleers et al have recently shown that PDC could produce higher amounts of interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) than islet cells, which could be helpful for revascularization in islet grafts.17 Conversely, PDC express tissue factor (TF) and cytokines/chemokines that may be detrimental to islet engraftment.18, 19 Thus, the contribution of PDC to islet graft needs to be better characterized.
Current release criteria for human islet cell products include islet purity and yield based on dithizone (DTZ) staining,20 which provides an estimate of endocrine cell mass in the preparation. The actual purity of islet preparations transplanted into patients averages 50–60%, indicating that a substantial mass of nonendocrine cells, mainly acinar cells and PDC, are also implanted. Notably, the current product-release criteria do not take into account nonendocrine cell quality and quantity, even though emerging data suggest their possible influence on clinical islet transplantation outcome.16 In addition, there is no reliable method available to evaluate PDC in the islet cell product. Consequently, only few transplant centers have performed pretransplant evaluation of PDC and no data are available regarding the effect of transplanted PDC on islet grafts.
In this study, we developed a novel method to evaluate PDC-specific viability and content based on the use of laser scanning cytometry (LSC/iCys) and flow cytometry (FACS). This assay extends and complements our previously reported method for the assessment of β-cell-specific viability and content in islet preparations.20 This method allows for the evaluation of cell-subset-specific viability (PDC and β-cells) simultaneously as well as for the detailed phenotypic analysis of PDC and acinar cells. We investigated PDC phenotype, viability and function in 161 human islet preparations. PDC content widely varied among islet preparations even though they had similar purity. PDC isolated from different density fractions showed wide variations in the viability and the production of proinflammatory cytokine/chemokine, VEGF and TF, which are relevant to islet transplantation outcomes.
MATERIALS AND METHODS
Human Islet Isolation
Islet isolations were performed using a modified automated method from pancreata preserved with University of Wisconsin solution alone or with preoxygenated two-layer perfluorocarbon at the Human Cell Processing Facility of the University of Miami School of Medicine.21, 22
Islets were purified using a semi-automated cell processor (Cobe 2991; COBE Laboratories Inc., Lakewood, CO, USA) in a refrigerated (4°C) room. The purification procedure consisted of a continuous gradient purification performed by loading 1.100 g/ml (osmolality 320–373 mOsm/kg/H2O) and 1.077 g/ml (285–327 mOsm/kg/H2O) Ficoll-based density gradients using a gradient maker device, followed by toploading of the pancreatic digest (≤20 ml of tissue/run) in UW solution.23 After 5 min of centrifugation at 2400 r.p.m., approximately 15 fractions were collected. Islet fractions were combined based on the purity assessed by DTZ. In general, the fractions with highest islet purity were collected from the low-density fractions 4–7 (density 1.081–1.085 g/ml). Medium and high-density islet fractions were combined with fractions 8–10 and 11–14 (1.86–1.090 and 1.091–1.095 g/ml, respectively).
Assessment of PDC Content Using LSC/iCys
Assessments of PDC content and viability were performed 48–72 h after isolation. Single cell suspensions were obtained by incubating aliquots of approximately 2000 islet equivalents (IEQ) in 1 ml Accutase (Innovative Cell Technologies Inc., San Diego, CA, USA) at 37°C for 10 min, as described.24 Dispersed cells were fixed on glass slides with 2.5% paraformaldehyde (Electron Microscopy Sciences, Washington, PA, USA). After incubating with Protein Block (Bio-Genex, San Ramon, CA, USA), cells underwent 2 h incubation with primary antibodies: mouse monoclonal anti-cytokeratin (CK)-19 (1:50; Dako North America Inc., Carpinteria, CA, USA); rabbit polyclonal anti-CK19 (KRT19; 1:200; Protein Tech Group Inc., Chicago, IL, USA); mouse monoclonal anti-CA19-9 (1:100; Novocastra, UK) and rabbit polyclonal anti-amylase (1:5000; Amy; Dako). A 1 h incubation with secondary antibodies included Alexa Fluor-488 goat anti-mouse IgG and Alexa Fluor-647 goat anti-rabbit IgG (1:200; Molecular Probes, Eugene, OR, USA). Nuclear staining was obtained with 4′,6-diamidino-2-phenylindole (DAPI; 16.7 μg/ml; Molecular Probes). Samples were analyzed using a LSC/iCys (CompuCyte, Cambrige, MALSC). Slides were scanned at × 40. Each fluorescent positive event was recorded using the argon/HeNe/violet laser. Single cells were identified and gated according to the DAPI staining area (Figure 1a). Cells from every subpopulation were visualized directly in the LSC/iCys by relocalization to confirm regular morphology (Figure 1c). A minimum of 5000 cells was acquired and analyzed for each sample.20
Assessment of PDC-Specific Viability
Single cell suspensions were incubated for 30 min at 37°C in phosphate-buffered saline without Ca2+ and Mg2+ with Newport Green PDX acetoxymethylether (NG; 1 μM) and tetramethylrhodamineethylester (TMRE; 100 ng/ml; both from Molecular Probes). Cells were incubated with anti-CA19-9 antibody (1:100) for 15 min followed by goat anti-mouse antibody (Alexa-647). After washing, cells were stained with 7-aminoactinomycin D (7-AAD; Molecular Probes), a marker of cell death. Cell suspensions were analyzed (minimum 3.0 × 104 events) using a FACScan cytometer with the CellQuest-pro software (Becton Dickinson, Mountain View, CA, USA). We have evaluated apoptosis selectively in the NGbright and CA19-9+ cells according to the scheme in Figure 3. After counting, dead cells (7-AAD+) were excluded from further analysis, live β-cells (NGbright) and PDC (CA19-9+) were analyzed for mitochondrial membrane potential (TMRE), which allows discriminating between healthy (TMRE+) and apoptotic (TMRE−) cells. The percentage of TMRE+ on each cell subset was decided based on the gate of TMRE unstained samples.
Delivery of Proapoptotic Stimuli to Islet and Nonendocrine Cells
Islets were treated with selected compounds for 24 h to induce apoptosis, as described,25 including the nitric oxide (NO) donor, S-Nitroso-N-acetyl-DL-penicillamine (0.5 mM; Sigma, St Louis, MO, USA); hydrogen peroxide (H2O2 200 μM; Sigma); or cytokine cocktail with IL-1-β (50 U/ml), TNF-α (1,000 U/ml) and IFN-γ (1000 U/ml). Hypoxic stress was induced by a 6-h incubation of islet aliquots as a pellet into a 15 ml conical tube filled with culture medium.20
PDC Sorting
Dispersed cells were incubated with anti-CA19-9 antibody for 30 min followed by a 10 min incubation with magnetic beads coated with an anti-mouse IgG (Miltenyi Biotec, Auburn, CA, USA). The cell suspension was passed through a MACS separation column (Miltenyi Biotec) to obtain positive selection of CA19-9+ cells. The efficiency of sorting was confirmed by FACS analysis.26
Production of Inflammatory Mediators and VEGF by PDC
During islet purification by density gradients, pancreatic tissue fractions with different densities (low=1.080–1.085, medium=1.086–1.090, high=1.091–1.095 g/ml) were collected. PDC were sorted from high- and low-density fractions obtained from five independent islet preparations. Enriched PDC (1 × 106 per ml) were cultured for 24 h.27 Concentrations of proinflammatory mediators (namely, IL-1-β, IL-6, IL-8, IFN-γ, MCP-1, MIP-1β and TNF-α) and VEGF in supernatants were determined using Multi-Plex cytokine kits following the manufacturer’s protocol (Bio-Plex; Bio-Rad Laboratories, Hercules, CA, USA). Additionally, PDC were homogenized, and TF measured by ELISA (Imubind Tissue Factor; American Diagnostica, Greenwich, CT, USA).25 The amount of cytokines/chemokines, VEGF and TF was normalized by total PDC protein content.
In Vitro Viability Assessment of Islet Preparations Cultured with Or Without PDC
Islet preparations (3000 IEQ, purity>95%) were cultured with or without sorted PDC for 48 h. The assessment of β-cell-specific viability was performed as described.24
In Vivo Assessment of Islet Potency in the Diabetic Nude Mouse Model
Animal protocols were approved by IACUC. Athymic nu/nu mice (Harlan Laboratories, Indianapolis, IN, USA) were rendered diabetic with Streptozotocin (200 mg/kg, single intravenous administration; Sigma). Diabetes occurrence (>300 mg/100 ml) was monitored by measurements of nonfasting blood glucose concentrations.
Human islets (2000 and 1000 IEQ, purity >95%) alone or with sorted PDC (3 × 106) were transplanted under the kidney capsule of diabetic immunodeficient mice as described.22 After transplantation, nonfasting blood glucose values were assessed to determine reversal of diabetes (defined as stable nonfasting blood glucose <200 mg/100 ml). Nephrectomy of the graft-bearing kidney was performed to exclude residual function of the native pancreas.22
Statistical Analysis
Data were analyzed using Excel for Windows software. Data are shown as mean±s.e.m. Statistical analyses were preformed using methods appropriate to each specific analysis including Student's t-tests for two sample comparisons of independent groups, and two-factor analysis of variance (ANOVA) for analyses of ductal and β-cell viability from different density fractions. Statistical significance was considered for P-values <0.05. Logistic regression was then used to explore the influence of the two factors separately.
RESULTS
Phenotpic Analysis of Nonendocrine Cells in Islet Prepatrations Using LSC/iCys
To assess the proportion of nonendocrine cells in islet preparations, Amy and CK19 were used for the identification of acinar cells and PDC, respectively. This led to the identification of five cell subpopulations (Amy−CK19negative, Amy+CK19negative, Amy−CK19dull, Amy+CK19bright, Amy−CK19bright; Figure 1b). Although LSC/iCys analysis clearly showed Amy+CK19negative cells indicating exocrine cells, another cell subset Amy+CK19bright was also identified in most of human islet preparations.
In order to characterize this Amy−CK19dull population, immunostaining for insulin, glucagon and somatostatin was combined with CK19. The LSC/iCys analysis revealed that α-cells and δ-cells, but not β-cells, do express CK19dull (Figure 1d–f). The proportion of β-, α-, δ-, Amy+CK19negative; Amy−CK19bright and Amy+CK19bright cells from 106 islet preparations were 21.1±8.7, 19.1±8.4, 3.8±2.1, 10.4±8.1, 10.4±8.2 and 6.4±3.2%, respectively. These results indicate that single staining for CK19 is not specific for evaluating PDC content in islet preparations, and that LSC/iCys analysis allows for detailed phenotypic analysis of endocrine and nonendocrine cell subsets.
CK19 is an intracellular protein and, therefore, fixation of cells is necessary for its detection by immunostaining precluding its application on live cells. The carbohydrate antigen 19-9 (CA19-9) has been described as a pan-ductal membrane antibody for human PDC.26 Therefore, we evaluated the expression of CK19bright cells in 34 islet preparations and compared it to that of CA19-9 using LSC/iCys. A positive correlation between CK19bright and CA19-9+ expression was observed (R2=0.8752, p=0.032; Figure 2a). Moreover, α-cells and δ-cells expressing CK19dull resulted negative to CA19-9, whereas a fraction of CA19-9+ cells expressed amylase (Figure 2b–d).
These results suggest that CA19-9 is a better maker for identifying PDC than CK19 in terms of excluding the contamination of α and δ cells from the analysis without double staining, and that even the single staining of CA19-9 could not exclude the double positive cells with amylase from the analysis for the identification of PDC.
We examined 203 purified fractions from 106 human islet preparations with different purities assessed by DTZ and compared this to PDC content by CA19-9 immunostaining. Although PDC content and purity by DTZ showed a rough correlation (R2=0.4574), the proportion of CA19-9+ cells in islet preparations widely varied even if they had similar purity assessed by DTZ (Figure 3). This variability might be due to the different ratio of acinar and ductal cells contained in the DTZ negative population. These results indicate that it might be difficult to evaluate PDC content by DTZ staining, whereas FACS analysis by CA19-9 antibody allowed us to precisely assess PDC content in islet preparations.
Analysis of PDC Viability Using CA19-9 and TMRE Staining
We have developed an analytical method to simultaneously assess the viability of PDC and β-cells as well as the content of both cells by FACS (Figure 4). Viable (nonapoptotic) β-cells and PDC were analyzed for mitochondrial membrane potential by TMRE.
To confirm the value of our refined PDC and β-cell viability assessment method, we evaluated the effects of hypoxic/starving conditions, reactive oxygen species (ROS), NO and cytokine cocktail (IL-1β, TNF-α and IFN-γ; Figure 5a–d). All these noxious conditions led to a measurable decrease in the proportion of viable PDC and β-cells. Moreover, we observed that PDC are more resistant than β-cells to noxious conditions (Figure 5). In this setting, the percentages of NGbright cells were not significantly different between pre and post treatments. The percentage of NGbright cells in pretreatment, after hypoxic/starving conditions, H2O2, NO and cytokine cocktail treatment were 32.7±4.2, 30.8±6.1, 33.6±7.3, 32.7±7.1 and 30.0±5.3%, respectively; P=n.s.
Analysis of PDC Viability with Different Degrees of Purity
We examined PDC and β-cell viability in 202 fractions from 124 human islet preparations. The average PDC viability was higher than that of β-cells (PDC vs β-cell: 75.5±13.9 and 62.7±18.7%, respectively; P<0.0001). There was no correlation between PDC and β-cell viability (R2=0.0078; Figure 6a). These results suggested that the assay of heterogeneous cell populations such as FDA/PI, which has been currently used for clinical islet transplantation, could not represent β-cell-specific viability.
We selected 31 islet preparations, which had different purity fractions from three different density layers (low=1.080–1.085, medium=1.086–1.090 and high=1.091–1.095 g/ml) for analysis. The islet purity for each density fraction range: 95–71% (high), 70–41 (medium) and 40–10% (low), respectively. PDC and β-cell viability in each fraction was analyzed by FACS (Figure 6b). The relative ratio of PDC and β-cell viability within medium- and high-density fractions was calculated in regards to that obtained in the low-density fraction. The highest PDC viability was observed in fractions obtained from high-density, and decreased in those from low-density (medium/low=1.15±0.33, P<0.05; high/low=1.31±0.53, P<0.05; Figure 6c). However, the analysis of β-cells showed that the relative viability in medium-density fractions was significantly higher, when compared to that of low- and high-density fractions (P<0.05, P<0.01, respectively) (Figure 6c). The islet sizes from each density layer were estimated using total IEQ/islet number and compared. Islet sizes from each gradient layer were comparable (high:medium:low=1.34±0.57:1.38±1.02:1.51±0.92, P=n.s.).
Cytokines/Chemokines, VEGF and TF Production from CA19-9+ Cells
To evaluate the function of PDC from different density fractions after purification, we sorted PDC using CA19-9 antibody. Sorted PDC were incubated in culture medium for 24 h and then supernatant was collected for the measurement of inflammatory mediators. Recovered cells were homogenized for the measurement of TF, which has been shown to be relevant to clinical outcomes.28, 29 Cytokine and chemokine production from PDC isolated from high-density fractions was higher, when compared to that of low-density fractions. Moreover, PDC isolated from high-density fractions produced significantly higher amounts of VEGF, which could be beneficial for revascularization after transplantation. The data suggest that PDC with higher viability can produce larger amounts of proinflammatory mediators and VEGF (Figure 7a, Table 1). On the other hand, although impure islet preparations from high-density layers produced higher amount of TF, a significant reduction in TF production was observed from PDC obtained from high-density fractions (Figure 7b and c). These results suggested that the proportion and viability of PDC in islet preparations might be considered as one of the important factors that could affect islet graft function.
Effects of PDC on Islet Potency
To evaluate the effect of PDC on islet potency in vitro, sorted PDC were cultured with pure islet preparations for 48 h (n=3). β-Cell-specific viability by FACS showed no significant difference in islets cultured with or without PDC (40.5±2.7, 37.5±1.8%, P=n.s, respectively).
Additionally, using chemically induced diabetic immunodeficient mice we performed transplantation of islets with or without PDC for three independent human islet preparations (Figure 8). There was no significant difference between experimental groups regarding the rate of and the time to diabetes reversal after transplantation in both islet masses utilized.
DISCUSSION
In this study, we demonstrated that the use of three different dyes (7-AAD, NG and TMRE) and one surface antibody (anti-CA19-9) provides precise and objective information on the viability and content of PDC as well as of β-cells. Our results showed that the content and viability of PDC in islet preparations extensively differed, and that PDC from high-density fractions produced higher amount of proinflammatory cytokine/chemokines and VEGF but lower TF when compared to low-density fractions. Our data suggest that the precise assessment of PDC in addition to β-cells in human islet cell products may be of assistance in developing strategies to enhance islet graft function.
Although the clinical outcomes of islet transplantation have significantly improved,1, 2, 3, 4 almost 90% of the recipients in recent trials required reintroduction of insulin within 5 years.5, 6 This currently limited success provides impetus for assessing possible reasons for such an outcome, and trying to make changes that can allow for long-term graft function. Multiple factors may have contributed to the limited function of transplanted islets in the clinical setting. It is conceivable that a reduction of islet cell regeneration potential may have occurred due to the adverse effect of immunosuppressive drugs,27, 30 the place of implantation (eg, liver, which may be unsuitable for regeneration) or/and the loss of β-cell precursors in islet preparations.
Insulin-secreting cells have been obtained in vitro from diverse sources, including embryonic stem cells.31, 32 Several studies suggest that somatic stem cells can give rise to insulin-producing cells, including hepatic oval cells,33 spleen-derived cells34 and marrow-derived cells.35 PDC are closely associated with β-cells in the human pancreas36 and have been shown to give rise to endocrine cells in both rodents and humans.10, 11, 12 Beneficial effects of PDC on islet cell viability have been described, which may be partially attributed to their ability of producing IGF-II.14, 15 On the other hand, PDC may have detrimental effects on islet engraftment and survival. Exposure of PDC to IL-1 β and interferon-γ (IFN-γ) can result in NO production in human islets infiltrated by cytokine-releasing immune cells.18 Moreover, contaminating PDC may contribute to early β-cell damage after intrahepatic islet transplantation through their expression of TF.19, 28, 29 Immunogenicity of PDC may also relate to the expression of CD40, a member of the TNF-receptor family that was initially described in B cells, activated monocytes, dendritic, endothelial and epithelial cells as well as fibroblasts.37, 38 Moreover, we recently found that purified pancreatic β-cells express a functional CD40 and that, following engagement with its ligand (CD154) results in the secretion of proinflammatory mediators including IL-6, IL-8, MCP-1 and MIP-1β.39, 40 The CD40-CD154 costimulation pathway plays a pivotal role in numerous T-cell-mediated inflammatory disorders.36, 37, 38, 41
Korbutt et al showed that the number of CK19+ cells in islet preparations correlated well with the long-term metabolic success of clinical islet transplantation.16 This phenomenon may result from a greater frequency of islet cell neogenesis from putative PDC precursors. Additionally, Pipeleer et al have shown that PDC might be a source of IL-8 and VEGF, which may be beneficial for the revascularization of islets after implantation.17 Human islet grafts include a sizable mass of nonendocrine tissue that is transplanted with endocrine cells, and that may contribute to the early and long-term outcome.4, 5 Rodent studies also suggest that the composition of islet grafts, including endocrine and nonendocrine cell subsets, may influence long-term metabolic function.18, 19
Before this study, there was no assessment method for the detailed characterization of PDC content and viability. Analysis of nonendocrine cells through LSC/iCys revealed that a substantial amount of Amy+ cells also expressed CK19, suggesting that a single staining with CK19 could lead to an overestimation of PDC numbers by also counting acinar cell subsets. Moreover, it appears difficult by means of conventional immunohistochemical techniques to distinguish CK19bright from CK19dull cells, which are mainly composed of α-cells and δ-cells.16 Conversely, the analysis performed by LSC/iCys allowed for an objective and detailed phenotypic analysis of higher numbers of cells in a relatively short time. Moreover, PDC viability might represent a critical variable for both inflammation and regenerative potential after transplantation. Our data indicate that there was wide variability regarding PDC viability based on the analysis of more than 200 human islet fractions. Interestingly, we observed that PDC with higher viability were obtained from high-density fractions (lower purity). In addition, higher cytokine/chemokine and VEGF levels were obtained from more viable PDC.
The need for reliable predictive islet cell product tests has prompted the development of multiple potency assays such as oxygen consumption rate,42, 43, 44 analysis of ATP levels45 and the measurement of ROS.46 All proposed methods have shown promise regarding their predictive value on graft function into chemically diabetic, immunodeficient mice receiving high purity human or porcine islets (the latter generally ∼80–90% β-cell content). However, clinical islet preparations are composed not only of β-cells, but also by a substantial amount of nonendocrine and endocrine (α- and δ-cells) cellular subsets. The most difficult part in islet potency testing is to identify β-cell-specific contribution to the results obtained in each of these tests. Notably, for potency assays that do not discriminate for cell subsets, only if each cell subset within the same islet preparation has similar viability, the result of the test may be representative to β-cell viability. According to the analysis of more than 160 islet preparations in our study, the viability and content of PDC and of β-cells widely varied and there was no correlation between PDC and β-cell values. These results clearly indicate that the assessment of heterogeneous cell populations such as ADP/ATP ratio and FDA/PI cannot represent β-cell-specific viability.
Another critical issue to be addressed in islet potency testing is whether the viability of islet aliquots obtained from the purest layers can safely represent that of an entire final islet preparation with lower purity. Our data showed that β-cell viability in medium- and high-density fractions was significantly higher and lower, respectively. In addition, high-density fractions showed the highest PDC viability, which decreased linearly in lower density fractions. These results indicate that the viability assessment of the purest islet fractions may not reflect adequately that of PDC or β-cells in whole islet preparations, and that the sample for islet quality assessment and β-cell viability should be obtained from a mixed fraction of the final islet cell product after mixing pure and impure fractions. Therefore, some islet quality tests evaluating whole islet cells (ie, FDA/PI and ADP/ATP ratio) may overestimate β-cell viability due to the contribution to the readout of PDC with higher viability composing the final preparation.
Although it is apparent that islet cell quality is critical for clinical outcomes, other factors such as inflammation mediators, VEGF and TF should be taken into account for overall assessment of islet cell products. Indeed, MCP-1 secreted by islet preparations may negatively affect the clinical outcomes47, 48 and TF may trigger detrimental thrombotic reactions at the time of intrahepatic islet infusion.28 However, VEGF and IL-8 may be beneficial for revascularization in islet grafts. In our study, we observed that PDC secrete proinflammatory cytokines/chemokines that PDC may have either beneficial or detrimental effects on islet viability and function. In the in vivo bioassay model, cotransplantation of PDC did not alter graft outcomes in our study. There was no measurable improvement of islet engraftment, despite an increased VEGF and IL-8 secretion from PDC in vitro that might have been beneficial. Notably, this immunodeficient murine model does not allow mimicking the complex immune phenomena ongoing in the setting of islet transplantation into patients with autoimmune diabetes. It is conceivable that lymphocytes and monocytes infiltrating islet grafts may be activated/recruited by cytokines/chemokines produced by PDC. Future efforts will focus on the effects of PDC on the function of islet grafts in clinical islet transplantation.
In summary, we have established a novel method for the simultaneous assessment of PDC and β-cell content and viability in human islet preparations. The precise assessment of nonendocrine cells in addition to β-cells in human islet cell products may be of assistance in developing strategies to enhance islet graft function.
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Acknowledgements
This work was supported in part by National Institutes of Health grants for General Clinical Research Center (5U01DK70460-02, 5R01DK025802-23), Islet Cell Resources (5U42RR016603), Juvenile Diabetes Research Foundation International (4-2004-361), and the Diabetes Research Institute Foundation (http://www.DiabetesResearch.org). We are grateful to the members of the Human Cell Processing Facility, Preclinical Cell Processing Laboratory of the Cell Transplant Center, Clinical Islet Transplant Program, General Clinical Research Center, Imaging Core at the Diabetes Research Institute, Administrative Offices at the Diabetes Research Institute and Organ Procurement Organizations for the continuous enthusiasm and support to our program.
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Ichii, H., Miki, A., Yamamoto, T. et al. Characterization of pancreatic ductal cells in human islet preparations. Lab Invest 88, 1167–1177 (2008). https://doi.org/10.1038/labinvest.2008.87
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DOI: https://doi.org/10.1038/labinvest.2008.87
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