Featured
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Research Highlights |
Metalloenzyme structures in a shot
Researchers study the structure of the metalloenzyme photosystem II by applying femtosecond X-ray pulses to simultaneously record X-ray diffraction and X-ray emission spectroscopy data.
- Allison Doerr
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Research Highlights |
Peering at protons
Unusual properties of a diamond defect are exploited to achieve progress toward nanometer-scale magnetic resonance imaging (MRI).
- Michael Eisenstein
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Tools in Brief |
RNA SHAPEs up
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News & Views |
Interpreting protein networks with three-dimensional structures
A fully automated pipeline that systematically models three-dimensional (3D) structural details of protein interactions will allow researchers to interpret perturbation effects within protein pathways and networks.
- Joan Teyra
- & Philip M Kim
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Brief Communication |
Correcting pervasive errors in RNA crystallography through enumerative structure prediction
A Rosetta algorithm improves RNA structure by scoring each nucleotide's possible conformations based on an energy function and electron-density data.
- Fang-Chieh Chou
- , Parin Sripakdeevong
- & Rhiju Das
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Methods in Brief |
Invisible excited-state RNA structures
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Research Highlights |
A genetically encoded probe for EM
Electron microscopy welcomes a versatile genetically encoded protein tag.
- Tal Nawy
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News & Views |
Six steps closer to FRET-driven structural biology
A new toolbox for structural biology that combines single-molecule fluorescence and molecular modeling is used to generate high-precision structures of protein complexes.
- Timothy D Craggs
- & Achillefs N Kapanidis
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Article |
Membrane-protein structure determination by solid-state NMR spectroscopy of microcrystals
The structure of the membrane anchor domain of the bacterial autotransporter YadA is solved by a solid-state NMR spectroscopy approach using a uniformly 13C- and 15N-labeled microcrystalline sample.
- Shakeel Ahmad Shahid
- , Benjamin Bardiaux
- & Dirk Linke
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Methods in Brief |
Watching proteins in motion
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Correspondence |
Prevention of overfitting in cryo-EM structure determination
- Sjors H W Scheres
- & Shaoxia Chen
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Brief Communication |
False discovery rate estimation for cross-linked peptides identified by mass spectrometry
xProphet, a tool using a target-decoy strategy, determines false discovery rate in mass-spectrometry data of chemically cross-linked peptides.
- Thomas Walzthoeni
- , Manfred Claassen
- & Ruedi Aebersold
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Tools in Brief |
Metallic electron microscopy
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News & Views |
Rendering RNA in 3D
Integrating biochemical footprinting data with molecular dynamics yields more accurate RNA three-dimensional structure predictions.
- Reza Behrouzi
- & Sarah A Woodson
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Article |
Facile backbone structure determination of human membrane proteins by NMR spectroscopy
The combination of cell-free protein expression and combinatorial dual labeling–aided NMR analysis allows for the rapid backbone structure assessment of human membrane proteins.
- Christian Klammt
- , Innokentiy Maslennikov
- & Senyon Choe
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Tools in Brief |
Tags to disentangle Dicer
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Research Highlights |
Etch-a-cell
A milling technique affords researchers a high-resolution glimpse deep into the cell using cryoelectron tomography.
- Allison Doerr
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Research Highlights |
How TAL effectors bind DNA
Structure of modular DNA-recognition domain is revealed.
- Monya Baker
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Tools in Brief |
Channelrhodopsin's crystal structure
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Research Highlights |
A bird's-eye view of disease
The integration of protein-protein interaction networks with structural information about the interacting protein partners creates a three-dimensional scaffold on which are mapped mutations involved in human disease.
- Natalie de Souza
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News & Views |
Small is beautiful
Using two independent methods, researchers show that in vivo–grown crystals of soluble proteins and of membrane proteins grown in the lipidic sponge phase can be analyzed by serial femtosecond crystallography on an X-ray free electron laser.
- Keith Moffat
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Brief Communication |
Lipidic phase membrane protein serial femtosecond crystallography
Lipidic sponge phase crystallization yields membrane protein microcrystals that can be injected into an X-ray free electron laser beam, yielding diffraction patterns that can be processed to recover the crystal structure.
- Linda C Johansson
- , David Arnlund
- & Richard Neutze
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Brief Communication |
In vivo protein crystallization opens new routes in structural biology
Expression of a protein in Sf9 insect cells at high concentration triggers formation of in vivo crystals that can be analyzed by serial femtosecond X-ray crystallography.
- Rudolf Koopmann
- , Karolina Cupelli
- & Michael Duszenko
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Brief Communication |
Blotting protein complexes from native gels to electron microscopy grids
A simple, general procedure for transferring protein complexes directly from native gels to electron microscopy grids for structural analysis is reported.
- Roland Wilhelm Knispel
- , Christine Kofler
- & Stephan Nickell
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News in Brief |
'Mutate-and-map' RNA structure characterization
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Review Article |
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy
In this Review, the authors thoroughly discuss solution-state NMR spectroscopy methods used for characterizing the dynamic structure landscape of RNA molecules, from picosecond to second timescale motions.
- Jameson R Bothe
- , Evgenia N Nikolova
- & Hashim M Al-Hashimi
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News in Brief |
High-resolution structures from low-resolution data
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Research Highlights |
Thwarting amyloid fibers
Two structure-driven studies of the culprits behind diseases associated with amyloid fibers give clues to stopping these agents in their tracks.
- Irene Kaganman
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Advertising Feature: Application Note |
mosquito® LCP: Making membrane protein crystallization accessible to the research scientist
- Wendy Gaisford
- , Gebhard Schertler
- & Pat Edwards
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Research Highlights |
Diffraction before destruction
Two recent reports demonstrate the first applications of X-ray free-electron lasers to look at biological structures.
- Allison Doerr
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Research Highlights |
The birth of a ribosome
A team of researchers applied a 'discovery single-particle profiling' experimental strategy to visualize the assembly of the ribosome via time-resolved electron microscopy.
- Allison Doerr
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Method to Watch |
Single-molecule structure determination
X-ray free-electron lasers may enable single-molecule structure determination.
- Allison Doerr
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News & Views |
The RNA structurome: high-throughput probing
Novel deep-sequencing strategies are used to monitor, at the genomic scale, the structure of cellular RNAs using enzymatic probing.
- Eric Westhof
- & Pascale Romby
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Correspondence |
Rapid readout detector captures protein time-resolved WAXS
- Sebastian Westenhoff
- , Erik Malmerberg
- & Richard Neutze
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Research Highlights |
Faster, sharper electron microscopy
A technique combining laser and electron pulses is used to achieve nanometer and femtosecond resolution in biological imaging.
- Monya Baker
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Research Highlights |
A database of dynamics
The Dynameomics database houses native-state and high-temperature unfolding simulation data for the 100 most populated protein folds; such information provides insights into protein function beyond structure alone.
- Allison Doerr
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News & Views |
Waltz, an exciting new move in amyloid prediction
A new amyloid-prediction tool, Waltz, offers advantages over previous amyloid-prediction tools for distinguishing 'true' amyloids from amorphous aggregates.
- Mikael Oliveberg
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Brief Communication |
Atomic accuracy in predicting and designing noncanonical RNA structure
A Rosetta full-atom framework, called fragment assembly of RNA with full-atom refinement (FARFAR), allows the de novo structure prediction and design of noncanonical RNA motifs with near-atomic resolution.
- Rhiju Das
- , John Karanicolas
- & David Baker
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Research Highlights |
One-shot structure determination
By sampling a two-dimensional diffraction pattern on a spherical detector, three-dimensional structure determination of single molecules should be possible from a single measurement.
- Allison Doerr