Structure determination

Definition

Structure determination is a procedure by which the three-dimensional atomic coordinates of a molecule or biomolecule are solved using an analytical technique. Many techniques are used in structure determination, most commonly X-ray crystallography, NMR spectroscopy, electron microscopy and molecular modelling.

Featured

Latest Research and Reviews

  • Research |

    The design and mutagenesis of an α-helix-containing monomeric miniprotein, PPα-Tyr, provide insights into weak noncovalent CH–π interactions that help define and stabilize folded proteins and protein–ligand interactions.

    • Emily G Baker
    • , Christopher Williams
    • , Kieran L Hudson
    • , Gail J Bartlett
    • , Jack W Heal
    • , Kathryn L Porter Goff
    • , Richard B Sessions
    • , Matthew P Crump
    •  & Derek N Woolfson
  • Research |

    The crystal structure of the full-length human glucagon receptor reveals the essential role of the 12-residue ‘stalk’ segment and an extracellular loop in the regulation of ligand binding and receptor activation.

    • Haonan Zhang
    • , Anna Qiao
    • , Dehua Yang
    • , Linlin Yang
    • , Antao Dai
    • , Chris de Graaf
    • , Steffen Reedtz-Runge
    • , Venkatasubramanian Dharmarajan
    • , Hui Zhang
    • , Gye Won Han
    • , Thomas D. Grant
    • , Raymond G. Sierra
    • , Uwe Weierstall
    • , Garrett Nelson
    • , Wei Liu
    • , Yanhong Wu
    • , Limin Ma
    • , Xiaoqing Cai
    • , Guangyao Lin
    • , Xiaoai Wu
    • , Zhi Geng
    • , Yuhui Dong
    • , Gaojie Song
    • , Patrick R. Griffin
    • , Jesper Lau
    • , Vadim Cherezov
    • , Huaiyu Yang
    • , Michael A. Hanson
    • , Raymond C. Stevens
    • , Qiang Zhao
    • , Hualiang Jiang
    • , Ming-Wei Wang
    •  & Beili Wu
  • Research |

    Crystal structures of the human GLP-1 receptor in complex with two negative allosteric modulators reveal a common binding pocket, and, together with mutagenesis and modelling studies, further our understanding of the receptor activation mechanism.Author: Please check the wording of the following statement, which will appear online only.

    • Gaojie Song
    • , Dehua Yang
    • , Yuxia Wang
    • , Chris de Graaf
    • , Qingtong Zhou
    • , Shanshan Jiang
    • , Kaiwen Liu
    • , Xiaoqing Cai
    • , Antao Dai
    • , Guangyao Lin
    • , Dongsheng Liu
    • , Fan Wu
    • , Yiran Wu
    • , Suwen Zhao
    • , Li Ye
    • , Gye Won Han
    • , Jesper Lau
    • , Beili Wu
    • , Michael A. Hanson
    • , Zhi-Jie Liu
    • , Ming-Wei Wang
    •  & Raymond C. Stevens

News and Comment