This Month |
Featured
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Research Highlight |
Atomic-level in-cell protein NMR
A newly developed method allows structure elucidation of proteins inside intact mammalian cells, using solid-state NMR spectroscopy.
- Arunima Singh
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Article |
Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis
A nuclear magnetic resonance spectroscopy-based approach to monitor multiple molecule and reaction types at once, Systems NMR, provides in vitro insights into complex biomolecular network dynamics.
- Yaroslav Nikolaev
- , Nina Ripin
- & Frédéric H.-T. Allain
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This Month |
Haribabu Arthanari
A spin-jock’s path to biomolecular dynamics with NMR, and why solitude matters.
- Vivien Marx
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Article |
Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics
The development of 19F-13C TROSY provides a new avenue for the collection of high-sensitivity, background-free information about the structure and dynamics of challenging biomolecular systems by NMR spectroscopy.
- Andras Boeszoermenyi
- , Sandeep Chhabra
- & Haribabu Arthanari
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Methods in Brief |
Solution-state 13C NMR gets a boost
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Brief Communication |
Structural modeling of protein–RNA complexes using crosslinking of segmentally isotope-labeled RNA and MS/MS
A mass spectrometry–based method to pinpoint UV-induced crosslinks in ribonucleoprotein complexes at protein residue and RNA nucleotide resolution provides key structural information for integrative modeling.
- Georg Dorn
- , Alexander Leitner
- & Frédéric H-T Allain
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Brief Communication |
Covalently circularized nanodiscs for studying membrane proteins and viral entry
Membrane proteins can be stabilized in a native-like setting using lipid-bilayer-based nanodiscs encircled by a membrane scaffold protein. Covalently circularized nanodiscs now offer enhanced stability and control over nanodisc diameter size, improving the quality of structural data.
- Mahmoud L Nasr
- , Diego Baptista
- & Gerhard Wagner
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Methods in Brief |
Faster spinning for better structure resolution
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Research Highlights |
Structure in the cellular context
Researchers develop an approach based on solid-state nuclear magnetic resonance (NMR) to study the structure of an intrinsically disordered protein under near-native conditions.
- Allison Doerr
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Methods in Brief |
RNA structure by solid-state NMR
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Research Highlights |
A machine to manage modifications
An automated platform mass-produces RNAs incorporating a wide variety of precisely positioned chemical alterations.
- Michael Eisenstein
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Brief Communication |
Protein structure determination by combining sparse NMR data with evolutionary couplings
A hybrid method that combines sparse NMR spectroscopy data with evolutionary residue-residue coupling information is used to solve accurate structures of large proteins.
- Yuefeng Tang
- , Yuanpeng Janet Huang
- & Gaetano T Montelione
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Brief Communication |
Homology modeling of larger proteins guided by chemical shifts
An NMR chemical shift–guided protein alignment method is combined with a comparative modeling pipeline to readily solve structures of large proteins.
- Yang Shen
- & Ad Bax
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Research Highlights |
Diamonds for MRI
Three groups report key steps toward nanometer-scale magnetic resonance imaging using nitrogen-vacancy centers in diamond.
- Allison Doerr
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Brief Communication |
Structure determination of noncanonical RNA motifs guided by 1H NMR chemical shifts
CS-Rosetta-RNA is a web tool for determining high-resolution structures of noncoding RNA motifs using 1H NMR chemical shift data coupled with Rosetta-based modeling.
- Parin Sripakdeevong
- , Mirko Cevec
- & Rhiju Das
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Article |
Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein
A strategy, based on solid-state NMR spectroscopy, for determining the structure of an oligomeric, seven-helix membrane protein (Anabaena sensory rhodopsin) in a lipid environment is described.
- Shenlin Wang
- , Rachel A Munro
- & Vladimir Ladizhansky
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Research Highlights |
Fingerprinting with MRI
A new approach to magnetic resonance imaging data acquisition and analysis promises to improve sensitivity and facilitate quantitative measurements.
- Allison Doerr
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Methods in Brief |
Observing a maturing protein
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Research Highlights |
Peering at protons
Unusual properties of a diamond defect are exploited to achieve progress toward nanometer-scale magnetic resonance imaging (MRI).
- Michael Eisenstein
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Methods in Brief |
Invisible excited-state RNA structures
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Article |
Membrane-protein structure determination by solid-state NMR spectroscopy of microcrystals
The structure of the membrane anchor domain of the bacterial autotransporter YadA is solved by a solid-state NMR spectroscopy approach using a uniformly 13C- and 15N-labeled microcrystalline sample.
- Shakeel Ahmad Shahid
- , Benjamin Bardiaux
- & Dirk Linke
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Methods in Brief |
Watching proteins in motion
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Brief Communication |
Reversible labeling of native and fusion-protein motifs
Removing phosphopantetheine-tagged labels from acyl carrier proteins (ACPs) and ACP fusion proteins contributes to a versatile labeling method in which tags can be iteratively swapped.
- Nicolas M Kosa
- , Robert W Haushalter
- & Michael D Burkart
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Article |
Facile backbone structure determination of human membrane proteins by NMR spectroscopy
The combination of cell-free protein expression and combinatorial dual labeling–aided NMR analysis allows for the rapid backbone structure assessment of human membrane proteins.
- Christian Klammt
- , Innokentiy Maslennikov
- & Senyon Choe
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News in Brief |
Dark-state exchange saturation transfer
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Review Article |
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy
In this Review, the authors thoroughly discuss solution-state NMR spectroscopy methods used for characterizing the dynamic structure landscape of RNA molecules, from picosecond to second timescale motions.
- Jameson R Bothe
- , Evgenia N Nikolova
- & Hashim M Al-Hashimi
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Research Highlights |
News in brief
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Research Highlights |
Protein structure gets exciting
Researchers determined the excited-state structure of a small protein using nuclear magnetic resonance spectroscopy.
- Allison Doerr
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Research Highlights |
NMR and the elusive GPCR
Researchers solve a high-resolution structure of a seven-helix transmembrane protein using nuclear magnetic resonance (NMR) spectroscopy.
- Allison Doerr
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Technology Feature |
Making membrane proteins for structures: a trillion tiny tweaks
Researchers try multiple means to get high-quality membrane proteins for X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy studies.
- Monya Baker
