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| Open AccessOptogenetically enhanced pituitary corticotroph cell activity post-stress onset causes rapid organizing effects on behaviour
Behavioural adaptations in response to stress are thought to be regulated by rapid neurotransmitter action, followed by slower hormonal signalling. Here, using optogenetic approaches, the authors find corticotroph cell populations are capable of rapidly modulating avoidance behaviours immediately after the onset of stress.
- Rodrigo J. De Marco
- , Theresa Thiemann
- & Soojin Ryu
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Article
| Open AccessThe oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway
Oncogenic mutations of isocitrate dehydrogenases 1 and 2 result in the production of the oncometabolite R-2-hydroxyglutarate. Here the authors show that the oncometabolite promotes mTOR activation in a PTEN/PI3K-independent manner by regulating DEPTOR stability via inhibition of KDM4A activity.
- Mélissa Carbonneau
- , Laurence M. Gagné
- & Frédérick A. Mallette
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Article
| Open AccessMultiple novel gene-by-environment interactions modify the effect of FTO variants on body mass index
Common variants in the fat mass and obesity associated (FTO) gene are linked to body mass index (BMI). Using the latest UK Biobank data, this study shows novel gene x environment interactions between FTOand lifestyle factors, including frequency of alcohol consumption, sleep, and added dietary salt.
- Alexander I. Young
- , Fabian Wauthier
- & Peter Donnelly
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Article
| Open AccessVascular microRNA-204 is remotely governed by the microbiome and impairs endothelium-dependent vasorelaxation by downregulating Sirtuin1
Commensal bacteria, the vast majority of which reside in the gut, are involved in development of many diseases, including atherosclerotic vascular disease. Here the authors show that these bacteria remotely increase expression of vascular microRNA-204, which targets Sirt1 in the endothelium to impair endothelial function.
- Ajit Vikram
- , Young-Rae Kim
- & Kaikobad Irani
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Article
| Open AccessNestin+ cells direct inflammatory cell migration in atherosclerosis
Bone marrow cells producing the intermediate filament nestin guide monocyte egress to the bloodstream in response to infection. Here, the authors show that nestin-producing stromal cells direct inflammatory cell migration in atherosclerosis, and that stromal Mcp1 is crucial in this process.
- Raquel del Toro
- , Raphael Chèvre
- & Simón Méndez-Ferrer
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Article
| Open AccessORP4L is essential for T-cell acute lymphoblastic leukemia cell survival
Lymphocytic leukaemia cells are characterized by high respiratory rates. Here, the authors report that the oxysterol-binding protein ORPL4 sustains mitochondrial respiration in T-cell acute lymphoblastic leukaemia cells by regulating Ca2+release from the endoplasmic reticulum.
- Wenbin Zhong
- , Qing Yi
- & Daoguang Yan
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Article
| Open AccessMARCH1 regulates insulin sensitivity by controlling cell surface insulin receptor levels
Insulin receptor levels at the cell surface are reduced in insulin resistance, for reasons that are not fully understood. Here, the authors identify the E3 ubiquitin ligase MARCH1 as a direct regulator of basal insulin receptor surface levels and, therefore, insulin signalling.
- Arvindhan Nagarajan
- , Max C. Petersen
- & Narendra Wajapeyee
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Article
| Open AccessThe aryl hydrocarbon receptor controls cyclin O to promote epithelial multiciliogenesis
Epithelia are barriers against environmental insults and express the transcription factor aryl hydrocarbon receptor (AhR). Here the authors show that AhR regulates multiciliogenesis via cyclin O and Multicilin in a Notch-dependent manner and that this is blocked by toxic ligands.
- Matteo Villa
- , Stefania Crotta
- & Andreas Wack
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Article
| Open AccessMURC deficiency in smooth muscle attenuates pulmonary hypertension
MURC protein regulates the function of caveolae, the small invaginations of the plasma membrane in muscle cells. Here the authors show that by interacting with caveolin proteins, MURC affects RhoA/ROCK signalling and regulates proliferation and migration of pulmonary artery smooth muscle cells, suggesting a new target in therapy of pulmonary hypertension.
- Naohiko Nakanishi
- , Takehiro Ogata
- & Tomomi Ueyama
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Article
| Open Accessβ-arrestin-2 regulates NMDA receptor function in spinal lamina II neurons and duration of persistent pain
The cellular mechanisms underlying acute pain transitions to chronic pain are poorly understood. Here the authors show that the scaffolding protein β-arrestin 2 contributes to these processes via desensitization of NMDA receptors in spinal neurons.
- Gang Chen
- , Rou-Gang Xie
- & Ru-Rong Ji
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Article
| Open AccessEndothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells
Pericytes and vascular smooth muscle cells are crucial for functional blood vessels, but the developmental sources of these cells are incompletely understood. Here, the authors show that endocardial endothelial cells give rise to cardiac mural cells, which are controlled by Wnt signalling.
- Qi Chen
- , Hui Zhang
- & Ralf H. Adams
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Article
| Open AccessCell-permeable succinate prodrugs bypass mitochondrial complex I deficiency
Mitochondrial complex I deficiency is the most common respiratory chain defect in mitochondrial disease in children and currently there is no effective treatment. In this study, the authors show that succinate prodrugs can alleviate metabolic decompensation in Leigh syndrome patient fibroblasts.
- Johannes K. Ehinger
- , Sarah Piel
- & Eskil Elmér
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Article
| Open AccessA critical role for NF2 and the Hippo pathway in branching morphogenesis
Branching morphogenesis is essential for the formation of most epithelial organs. Here, the authors show that Neurofibromatosis 2 (NF2), the Hippo pathway kinases LATS1 and LATS2, and the transcriptional co-activators YAP and TAZ control tip identity, RET signalling and branching morphogenesis in the mouse kidney.
- Antoine Reginensi
- , Leonie Enderle
- & Helen McNeill
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Article
| Open AccessThe transcriptional coregulator GRIP1 controls macrophage polarization and metabolic homeostasis
GRIP1 cooperates with the glucocorticoid receptor to repress inflammatory genes. Here the authors show that GRIP1 also controls macrophage polarization, by promoting KLF4-driven activation in response to IL-4, and that mice lacking GRIP1 in macrophages develop severe metabolic dysfunction on a high-fat diet.
- Maddalena Coppo
- , Yurii Chinenov
- & Inez Rogatsky
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Article
| Open AccessANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression
Angiopoietin-like 4 protein (ANGPTL4) is a regulator of lipoprotein metabolism whose role in atherosclerosis has been controversial. Here the authors show that ANGPTL4 deficiency in haematopoietic cells increases atherogenesis by promoting myeloid progenitor cell expansion and differentiation, foam cell formation and vascular inflammation.
- Binod Aryal
- , Noemi Rotllan
- & Carlos Fernández-Hernando
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Article
| Open AccessLkb1 controls brown adipose tissue growth and thermogenesis by regulating the intracellular localization of CRTC3
The kinase Lkb1 is expressed in various metabolic tissues and is known to regulate cellular and systemic energy homeostasis. Here, the authors delete Lkb1 specifically in mature adipocytes of mice to show that Lkb1 regulates brown adipose tissue expansion and expression of UCP1.
- Tizhong Shan
- , Yan Xiong
- & Shihuan Kuang
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Article
| Open AccessHealthy ageing of cloned sheep
Since the birth of the first cloned animal, Dolly the sheep, concerns have been raised about potential long-term health consequences of cloning. Here the authors report on a cohort of 13 aged cloned sheep, including four created from the same cells as Dolly, and find they are healthy and seem to age normally.
- K. D. Sinclair
- , S. A. Corr
- & D. S. Gardner
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| Open AccessGenetic lineage tracing defines myofibroblast origin and function in the injured heart
The origin and fate of myofibroblasts, the cells responsible for cardiac remodelling and fibrosis, is controversial. Here the authors show that cardiac myofibroblasts express periostin, derive exclusively from tissue-resident fibroblasts, are necessary for scar formation after injury, and can revert back to a less-activated state upon injury resolution.
- Onur Kanisicak
- , Hadi Khalil
- & Jeffery D. Molkentin
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Article
| Open AccessGlycolytic regulation of cell rearrangement in angiogenesis
Glycolytic regulator PFKFB3 is a key player in vessel sprouting. Here the authors develop a computational model predicting that PFKFB3 drives endothelial cell rearrangement during vessel sprouting by promoting filopodia formation and reducing intercellular adhesion, and empirically validate this prediction.
- Bert Cruys
- , Brian W. Wong
- & Peter Carmeliet
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Article
| Open AccessRegulation of post-Golgi LH3 trafficking is essential for collagen homeostasis
Lysine hydroxylation of procollagen precursors by LH3 is required for collagen fibril crosslinking and stabilization. Here the authors show that the trafficking protein VIPAR is required for correct sorting of LH3 into post-Golgi collagen carriers and for correct collagen modification and structure.
- Blerida Banushi
- , Federico Forneris
- & Paul Gissen
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Article
| Open AccessStructural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization
Sperm-egg fusion requires the interaction between IZUMO1 on the sperm and JUNO on the egg. Here, the authors report the crystal structure of mouse JUNO, and use it to explain its lack of binding to folate, along with in vivofunctional analyses.
- Kazuki Kato
- , Yuhkoh Satouh
- & Osamu Nureki
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Article
| Open AccessSeptin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV
The accumulation of lipid droplets is often observed in hepatitis C virus infection, but the mechanism of their formation is not known. Here the authors show that septin 9 expression is increased in infected livers, and a septin 9/phosphatidylinositol 5-phosphate signalling pathway regulates the growth of lipid droplets.
- Abdellah Akil
- , Juan Peng
- & Ama Gassama-Diagne
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Article
| Open AccessSREBP1c-CRY1 signalling represses hepatic glucose production by promoting FOXO1 degradation during refeeding
The clock protein Cry regulates hepatic glucose metabolism. Here the authors show that SREBP1c, activated by insulin signalling after feeding, directly regulates Cry transcription at specific circadian time points, and that Cry represses hepatic glucose production by promoting proteasomal degradation of Foxo1.
- Hagoon Jang
- , Gha Young Lee
- & Jae Bum Kim
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Article
| Open AccessTRPC5 channels participate in pressure-sensing in aortic baroreceptors
The identity of mechanosensors within aortic baroreceptors that sense fluctuations in blood pressure is unclear. Here, Lau et al.show that a cation channel TRPC5 acts as a transducer of mechanical stimuli in aortic baroreceptor neurons in rodents.
- On-Chai Lau
- , Bing Shen
- & Xiaoqiang Yao
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Article
| Open AccessCritical role of RanBP2-mediated SUMOylation of Small Heterodimer Partner in maintaining bile acid homeostasis
As signalling molecules, bile acids (BAs) can affect metabolism, but due to detergent-like properties, BA levels must be tightly regulated. Here, Kim et al.show that RanBP2, a nucleoporin, maintains BA homoeostasis through SUMOylation of Small Heterodimer Partner (SHP), an orphan nuclear receptor.
- Dong-Hyun Kim
- , Sanghoon Kwon
- & Jongsook Kim Kemper
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Article
| Open AccessNotch-independent RBPJ controls angiogenesis in the adult heart
Heart function after injury improves upon formation of new blood vessels. Here, the authors show that ablating a transcription factor RBPJ in the murine heart increases vascularization and maintains cardiac function after injury by increasing responsiveness to hypoxia, suggesting a new approach to treat heart injury.
- Ramón Díaz-Trelles
- , Maria Cecilia Scimia
- & Mark Mercola
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| Open AccessCoronary vasculature patterning requires a novel endothelial ErbB2 holoreceptor
Semaphorin ligands and cognate receptors are important in patterning the vasculature. Here, Aghajanian et al.report an unexpected role for ErbB2 in endothelial cells where it partners with Nrp1 to form a novel semaphoring holoreceptor required for embryonic vascular patterning.
- Haig Aghajanian
- , Young Kuk Cho
- & Jonathan A. Epstein
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Article
| Open AccessThe genetic regulatory signature of type 2 diabetes in human skeletal muscle
More than 90% of genetic variants associated with type 2 diabetes occur in non-coding regions. Scott et al. report genomes, epigenomes and transcriptomes of skeletal muscle from 271 participants with a range of glucose tolerances, revealing a genetic regulatory architecture enriched in muscle stretch/super enhancers.
- Laura J. Scott
- , Michael R. Erdos
- & Stephen C. J. Parker
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Article
| Open AccessMLP and CARP are linked to chronic PKCα signalling in dilated cardiomyopathy
Altered function of the muscle LIM protein (MLP) causes dilated cardiomyopathy in mice and humans. Lange et al. explain the molecular role of MLP in the heart by showing that it affects the signalling complex at the intercalated discs of failing hearts that consists of PKCα, PLCβ1 and CARP by inhibiting PKCα auto-phosphorylation and function.
- Stephan Lange
- , Katja Gehmlich
- & Elisabeth Ehler
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Article
| Open AccessRETRACTED ARTICLE: SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin
Ceruloplasmin has an important role in the stabilization and nuclear transport of HIF-1α, thus regulating VEGF expression. Here the authors show that the transcription factor SARI reduces colorectal cancer growth and angiogenesis in vivoby inducing the degradation of ceruloplasmin, thereby inhibiting the HIFα/VEGF axis.
- Lei Dai
- , Xueliang Cui
- & Hongxin Deng
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Article
| Open AccessCardiac sodium channel palmitoylation regulates channel availability and myocyte excitability with implications for arrhythmia generation
Cardiac voltage-gated sodium channels (Nav1.5) are crucial regulators of heart electric activity. Here the authors show that palmitoylation, a process of lipid modification of cysteine residues, modulates Nav1.5 function and affects cardiomyocyte excitability, representing a potential target in treating cardiac diseases.
- Zifan Pei
- , Yucheng Xiao
- & Theodore R. Cummins
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Article
| Open AccessThe necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance
The kinase RIPK3 initiates necroptosis, which has been reported to promote inflammation in various pathological conditions. Here, the authors show that genetic ablation of Ripk3results in adipocyte apoptosis and white adipose tissue inflammation in obese mice, which promotes glucose intolerance.
- Jérémie Gautheron
- , Mihael Vucur
- & Tom Luedde
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Article
| Open AccessOncogenic PIK3CA mutations reprogram glutamine metabolism in colorectal cancer
Cancer cells rely on glutamine to replenish the TCA cycle. Here, the authors show that oncogenic PIK3CAmutations drive this metabolic rewiring in colorectal cancer cells by up-regulating glutamate pyruvate transaminase expression, thus increasing sensitivity to glutamine starvation.
- Yujun Hao
- , Yardena Samuels
- & Zhenghe Wang
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Article
| Open AccessMale care and life history traits in mammals
Males help care for offspring in about 10% of mammal species. Here, West and Capellini perform phylogenetic comparative analyses on a sample of 529 mammal species and find that male care is associated with shorter lactation periods by females, larger litters of offspring, and more frequent breeding events.
- Hannah E. R. West
- & Isabella Capellini
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| Open AccessAge differences in learning emerge from an insufficient representation of uncertainty in older adults
The ability to learn decreases with old age especially in a dynamically changing environment, however the precise nature of this decline is not understood. Nassar and colleagues report that older adults show a reduced ability to learn from uncertain outcomes compared to younger adults.
- Matthew R. Nassar
- , Rasmus Bruckner
- & Ben Eppinger
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| Open AccessThe MDM2–p53–pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells
Mice lacking the tumour suppressor p53 are partially protected from developing diabetes. Here the authors show that p53 is upregulated in the pancreas of diabetic mice where it impairs β cell function by repressing expression of mitochondrial pyruvate carboxylase, thereby inhibiting insulin secretion.
- Xiaomu Li
- , Kenneth K. Y. Cheng
- & Aimin Xu
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Article
| Open AccessRebalancing gene haploinsufficiency in vivo by targeting chromatin
Deficit in transcription factor Tbx1 causes heart defects in humans and mice. Here the authors show that Tbx1 regulates gene expression by recruiting histone methyltransferases that affect chromatin marks, and that a drug inhibiting histone demethylation ameliorates the cardiovascular phenotype in Tbx1 haploinsufficient or hypomorphic mice.
- Filomena Gabriella Fulcoli
- , Monica Franzese
- & Antonio Baldini
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Article
| Open AccessThe ubiquitin E3 ligase TRAF6 exacerbates pathological cardiac hypertrophy via TAK1-dependent signalling
TRAF6 is a ubiquitin E3 ligase regulating a number of biological processes. Here the authors show that ROS, generated during pathological cardiac stress, induces TRAF6 auto-ubiquitination and activation, promoting its interaction with and ubiquitination of TAK1 that contributes to development of cardiac hypertrophy.
- Yan-Xiao Ji
- , Peng Zhang
- & Hongliang Li
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Article
| Open AccessAdaptive evolution of complex innovations through stepwise metabolic niche expansion
A fundamental question in evolutionary biology is how complex innovations requiring multiple genetic changes arise. Here the authors provide lines of evidence that changing environments facilitate the adaptive evolution of complex metabolic innovations via stepwise acquisition of single reactions.
- Balázs Szappanos
- , Jonathan Fritzemeier
- & Balázs Papp
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Article
| Open AccessVascular control of the Drosophila haematopoietic microenvironment by Slit/Robo signalling
The posterior signalling centre (PSC) in Drosophilalarva regulates blood cell differentiation but it is unclear how this is controlled. Here, the authors show that Slit/Robo signalling from the vascular system regulates PSC morphology and function, in turn, regulating blood cell differentiation.
- Ismaël Morin-Poulard
- , Anurag Sharma
- & Michèle Crozatier
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Article
| Open AccessFitness consequences of artificial selection on relative male genital size
Within species, there tends to be a tight relationship between genital size and body size, suggesting strong stabilizing selection. Here, Booksmythe et al.artificially select relative genital size in mosquitofish and find that novel genital size-body size combinations do not lead to expected fitness reductions.
- Isobel Booksmythe
- , Megan L. Head
- & Michael D. Jennions
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Article
| Open AccessHIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity
Migration to the inflamed tissue demands energy production in an increasingly hypoxic environment. Here the authors show that during migration, HIF1α-induced PDK1 uniquely adapts macrophage metabolism to mild hypoxia by promoting glycolysis while preserving cytochrome c oxidase activity.
- Hiroaki Semba
- , Norihiko Takeda
- & Issei Komuro
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Article
| Open AccessOptoDyCE as an automated system for high-throughput all-optical dynamic cardiac electrophysiology
The efficiency of preclinical drug testing and characterization of cellular function can be improved through the use of optogenetic tools. Here Klimas et al. present and validate OptoDyCE, a fully automated system for all-optical high-throughput cardiac electrophysiology.
- Aleksandra Klimas
- , Christina M. Ambrosi
- & Emilia Entcheva
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Article
| Open AccessArrhythmia risk stratification of patients after myocardial infarction using personalized heart models
Sudden arrhythmic death is a leading cause of mortality, however approaches to identify at-risk patients are of low sensitivity and specificity. Here, the authors develop a personalized approach to assess arrhythmia risk in post-infarction patients based on cardiac imaging and computational modelling that significantly outperforms existing clinical metrics.
- Hermenegild J. Arevalo
- , Fijoy Vadakkumpadan
- & Natalia A. Trayanova
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Article
| Open AccessMyoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression
Heart failure is a major public health issue but due to our poor disease understanding the current therapies are symptomatic. Here the authors identify Myoscape as a novel cardiac protein regulating membrane localization of the L-type calcium channel and heart's contractile force, thus promising new therapeutic avenues for heart failure.
- Matthias Eden
- , Benjamin Meder
- & Norbert Frey
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Article
| Open AccessExosomal microRNA miR-92a concentration in serum reflects human brown fat activity
Exosomes are RNA-containing lipid vesicles with roles in inter-tissue crosstalk. Here the authors show that exosome release from brown adipocytes is increased upon thermogenic activation, both in vitro and in vivo, and demonstrate that serum levels of exosomal miR-92 reflect brown fat activity in humans.
- Yong Chen
- , Joschka J. Buyel
- & Alexander Pfeifer
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Article
| Open AccessThe obesity-induced transcriptional regulator TRIP-Br2 mediates visceral fat endoplasmic reticulum stress-induced inflammation
Visceral and subcutaneous fat are associated with different metabolic risk, but mediators of such depot specific effects are not very well known. Here the authors identify the transcriptional regulator, TRIP-Br2, as a regulator of endoplasmic reticulum (ER) stress-induced inflammatory responses specifically in visceral fat.
- Guifen Qiang
- , Hyerim Whang Kong
- & Chong Wee Liew
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Article
| Open AccessAdipose tissue mTORC2 regulates ChREBP-driven de novo lipogenesis and hepatic glucose metabolism
The kinase mTOR controls anabolic metabolism. Here, the authors create fat-specific mTORC2 knockout mice using the Adiponectin-Cre driver and show mTORC2 signalling is important for systemic metabolic homeostasis by controlling adipocyte de novolipogenesis and glucose uptake.
- Yuefeng Tang
- , Martina Wallace
- & David A. Guertin
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Article
| Open AccessA two decade dementia incidence comparison from the Cognitive Function and Ageing Studies I and II
Future dramatic rises in dementia are widely reported, assuming no change in incidence. Matthews and colleagues report that, in contrast to such statements, age-specific incidence has dropped over 20 years, with overall incidence of dementia remaining stable in a large multi-site population study from England.
- F. E. Matthews
- , B. C. M. Stephan
- & G. Forster