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Microbial communities are groups of microorganisms that share a common living space. The microbial populations that form the community can interact in different ways, for example as predators and prey or as symbionts.
This protocol provides a computational pipeline for accurate detection and grouping of viral sequences from microbiome samples. The approach uses a set of viral protein families as bait for identifying viral sequences directly from metagenomic assemblies.
Enhanced conversion of dietary cholesterol to bile acids through the alternative pathway leads to cold-associated, metabolically beneficial changes in the intestinal microbiome and to elevated bile acid levels that contribute to adaptive thermogenesis.