Volume 17 Issue 7, July 2022

This protocol describes all-optical interrogation experiments in awake, behaving mice, demonstrating the utility of this strategy in three brain areas—barrel cortex, visual cortex and hippocampus—by using different experimental setups.

In this work, ‘hyperpolarized water’ produced by cryogenic dynamic nuclear polarization is used to boost signal intensities in NMR of proteins and nucleic acids. This allows NMR at lower concentrations and detection of folding intermediates.

Mouse or human organoids are generated from two cervical regions: columnar epithelium-lined endocervix and stratified squamous epithelium-lined ectocervix. Genetic manipulation for modeling cancers and organoid infections is also described.

This protocol details the synthesis and use of Peroxymycin-1, an activity-based histochemical probe for hydrogen peroxide detection in fixed cell and mouse tissue samples.

This protocol guides the user through normative modeling analysis using the Predictive Clinical Neuroscience toolkit (PCNtoolkit), enabling individual differences to be mapped at the level of a single subject or observation in relation to a reference model.