Volume 17 Issue 8, August 2022

Sulc and colleagues describe a design tool, oxView, for visualization and analysis of simulations of DNA, RNA and nucleic acid–protein nanostructures.

Fluorescently labeled nuclei of the enteric nervous system are isolated from adult mouse or zebrafish gut and characterized by nuclear RNA sequencing and visualization and quantification of transcripts using a custom analytical pipeline.

This protocol describes the preparation of two lipid-based membrane–core nanoformulations for FOLFOX by using the nanoprecipitation technique. These can be used in the treatment of colorectal cancer and hepatocellular carcinoma.

It is crucial to know the localization of a protein to fully understand its function. SubCellBarCode is a wet lab–, mass spectrometry- and bioinformatics-based pipeline that generates proteome-wide protein subcellular localization for human cancer cell lines.

This protocol enables users to establish long-term multigenerational ctenophore cultures in the laboratory and details CRISPR–Cas9 genome editing in Mnemiopsis leidyi.