Volume 17 Issue 6, June 2022

This protocol provides steps for DNA amplicon sequencing using miniaturized laboratory equipment for rapid in situ identification of biological specimens in environments such as local institutions, field stations and classrooms.

This protocol describes enrichment of telomeric repeats from human and mouse cells by successive rounds of restriction digestion and size fractionation, resulting in high-quality preparations suitable for single-molecule and structural studies.

Mice undergo subretinal injection, with successful injection confirmed by follow-up examination of the retina and the retinal pigmented epithelium.

This protocol combines in situ chromosome conformation capture with labeling of nascent DNA with the synthetic nucleoside 4-thio-thymidine to generate genome-wide contact probability maps within and across sister chromatids in mammalian cells.

A protocol for generating chromatin accessibility profiles from a broad variety of cell and tissue types, including a step-by-step workflow for library preparation and guidelines for data processing and downstream analysis.