Latest Protocols
Volume 3 No 12
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Nature protocols
Nonradioactive enzymatic assay for plasma and serum vitamin B6 - pp1815 - 1819
Qinghong Han & Robert M Hoffman
Published online: 06 November 2008 | doi:10.1038/nprot.2008.181
Abstract - Nonradioactive enzymatic assay for plasma and serum vitamin B: 6 | Full Text - Nonradioactive enzymatic assay for plasma and serum vitamin B6 | PDF (215 KB) - Nonradioactive enzymatic assay for plasma and serum vitamin B6
Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates - pp1820 - 1831
Sherif S Ebada, Ru Angelie Edrada, Wenhan Lin & Peter Proksch
Published online: 06 November 2008 | doi:10.1038/nprot.2008.182
Abstract - Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates | Full Text - Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates | PDF (988 KB) - Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates
SCWRL and MolIDE: computer programs for side-chain conformation prediction and homology modeling - pp1832 - 1847
Qiang Wang, Adrian A Canutescu & Roland L Dunbrack, Jr
Published online: 06 November 2008 | doi:10.1038/nprot.2008.184
Abstract - SCWRL and MolIDE: computer programs for side-chain conformation prediction and homology modeling | Full Text - SCWRL and MolIDE: computer programs for side-chain conformation prediction and homology modeling | PDF (1,166 KB) - SCWRL and MolIDE: computer programs for side-chain conformation prediction and homology modeling
Synthesis of biotin–AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription - pp1848 - 1861
Faqing Huang, Jun He, Yilin Zhang & Yanlin Guo
Published online: 06 November 2008 | doi:10.1038/nprot.2008.185
Abstract - Synthesis of biotin-AMP conjugate for 5[prime] biotin labeling of RNA through one-step : in vitro: transcription | Full Text - Synthesis of biotin–AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription | PDF (480 KB) - Synthesis of biotin–AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription
Methods for generating and colonizing gnotobiotic zebrafish - pp1862 - 1875
Linh N Pham, Michelle Kanther, Ivana Semova & John F Rawls
Published online: 13 November 2008 | doi:10.1038/nprot.2008.186
Abstract - Methods for generating and colonizing gnotobiotic zebrafish | Full Text - Methods for generating and colonizing gnotobiotic zebrafish | PDF (351 KB) - Methods for generating and colonizing gnotobiotic zebrafish
A protocol for unraveling gene regulatory networks - pp1876 - 1887
Stefan C Materna & Paola Oliveri
Published online: 13 November 2008 | doi:10.1038/nprot.2008.187
Abstract - A protocol for unraveling gene regulatory networks | Full Text - A protocol for unraveling gene regulatory networks | PDF (337 KB) - A protocol for unraveling gene regulatory networks
Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale - pp1888 - 1894
Myung-Soo Cho, Dong-Youn Hwang & Dong-Wook Kim
Published online: 13 November 2008 | doi:10.1038/nprot.2008.188
Abstract - Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale | Full Text - Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale | PDF (535 KB) - Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale
A sensitive recombinant cell-based bioluminescent assay for detection of androgen-like compounds - pp1895 - 1902
Elisa Michelini, Luca Cevenini, Laura Mezzanotte, Piia Leskinen, Marko Virta, Matti Karp & Aldo Roda
Published online: 20 November 2008 | doi:10.1038/nprot.2008.189
Abstract - A sensitive recombinant cell-based bioluminescent assay for detection of androgen-like compounds | Full Text - A sensitive recombinant cell-based bioluminescent assay for detection of androgen-like compounds | PDF (324 KB) - A sensitive recombinant cell-based bioluminescent assay for detection of androgen-like compounds
Methylation-sensitive high-resolution melting - pp1903 - 1908
Tomasz K Wojdacz, Alexander Dobrovic & Lise Lotte Hansen
Published online: 20 November 2008 | doi:10.1038/nprot.2008.191
Abstract - Methylation-sensitive high-resolution melting | Full Text - Methylation-sensitive high-resolution melting | PDF (280 KB) - Methylation-sensitive high-resolution melting
In vitro growth and analysis of Candida biofilms - pp1909 - 1924
Jyotsna Chandra, Pranab K Mukherjee & Mahmoud A Ghannoum
Published online: 20 November 2008 | doi:10.1038/nprot.2008.192
Abstract - : In vitro: growth and analysis of : Candida: biofilms | Full Text - In vitro growth and analysis of Candida biofilms | PDF (1,127 KB) - In vitro growth and analysis of Candida biofilms
A C. elegans-based, whole animal, in vivo screen for the identification of antifungal compounds - pp1925 - 1931
Emmanouil Tampakakis, Ikechukwu Okoli & Eleftherios Mylonakis
Published online: 20 November 2008 | doi:10.1038/nprot.2008.193
Abstract - A : C. elegans: -based, whole animal, : in vivo: screen for the identification of antifungal compounds | Full Text - A C. elegans-based, whole animal, in vivo screen for the identification of antifungal compounds | PDF (256 KB) - A C. elegans-based, whole animal, in vivo screen for the identification of antifungal compounds
In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood - pp1932 - 1940
Christopher Y Park, Ravindra Majeti & Irving L Weissman
Published online: 20 November 2008 | doi:10.1038/nprot.2008.194
Abstract - : In vivo: evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood | Full Text - In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood | PDF (365 KB) - In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood
SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs - pp1941 - 1974
Tanvir R Shaikh, Haixiao Gao, William T Baxter, Francisco J Asturias, Nicolas Boisset, Ardean Leith & Joachim Frank
Published online: 04 December 2008 | doi:10.1038/nprot.2008.156
Abstract - SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs | Full Text - SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs | PDF (3,304 KB) - SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs | Supplementary information
Expression cloning and radiotracer uptakes in Xenopus laevis oocytes - pp1975 - 1980
Daniel Markovich
Published online: 04 December 2008 | doi:10.1038/nprot.2008.151
Abstract - Expression cloning and radiotracer uptakes in : Xenopus: laevis oocytes | Full Text - Expression cloning and radiotracer uptakes in Xenopus laevis oocytes | PDF (366 KB) - Expression cloning and radiotracer uptakes in Xenopus laevis oocytes
Cryopreservation and banking of mammalian cell lines - pp1981 - 1989
Glyn N Stacey & John R Masters
Published online: 04 December 2008 | doi:10.1038/nprot.2008.190
Abstract - Cryopreservation and banking of mammalian cell lines | Full Text - Cryopreservation and banking of mammalian cell lines | PDF (286 KB) - Cryopreservation and banking of mammalian cell lines
Dual fluorescence labeling of surface-exposed and internal proteins in erythrocytes infected with the malaria parasite Plasmodium falciparum - pp1990 - 1996
Dominique C Bengtsson, Kordai M P Sowa & David E Arnot
Published online: 04 December 2008 | doi:10.1038/nprot.2008.196
Abstract - Dual fluorescence labeling of surface-exposed and internal proteins in erythrocytes infected with the malaria parasite Plasmodium falciparum | Full Text - Dual fluorescence labeling of surface-exposed and internal proteins in erythrocytes infected with the malaria parasite Plasmodium falciparum | PDF (346 KB) - Dual fluorescence labeling of surface-exposed and internal proteins in erythrocytes infected with the malaria parasite Plasmodium falciparum
A protocol for studying the kinetics of RNA within cultured cells: application to ribosomal RNA - pp1997 - 2004
Marc Thiry, Françoise Lamaye, Nicolas Thelen, Aurore Chatron-Colliet, Nathalie Lalun, Hélène Bobichon & Dominique Ploton
Published online: 04 December 2008 | doi:10.1038/nprot.2008.198
Abstract - A protocol for studying the kinetics of RNA within cultured cells: application to ribosomal RNA | Full Text - A protocol for studying the kinetics of RNA within cultured cells: application to ribosomal RNA | PDF (506 KB) - A protocol for studying the kinetics of RNA within cultured cells: application to ribosomal RNA | Supplementary information
