Digital production, transmission and storage have revolutionized how we access and use information but have also made archiving an increasingly complex task that requires active, continuing maintenance of digital media. This challenge has focused some interest on DNA as an attractive target for information storage1 because of its capacity for high-density information encoding, longevity under easily achieved conditions2, 3, 4 and proven track record as an information bearer. Previous DNA-based information storage approaches have encoded only trivial amounts of information5, 6, 7 or were not amenable to scaling-up8, and used no robust error-correction and lacked examination of their cost-efficiency for large-scale information archival9. Here we describe a scalable method that can reliably store more information than has been handled before. We encoded computer files totalling 739 kilobytes of hard-disk storage and with an estimated Shannon information10 of 5.2 × 106 bits into a DNA code, synthesized this DNA, sequenced it and reconstructed the original files with 100% accuracy. Theoretical analysis indicates that our DNA-based storage scheme could be scaled far beyond current global information volumes and offers a realistic technology for large-scale, long-term and infrequently accessed digital archiving. In fact, current trends in technological advances are reducing DNA synthesis costs at a pace that should make our scheme cost-effective for sub-50-year archiving within a decade.
At a glance
Sequence Read Archive
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- Supplementary Information 1 (1.9M)
This file contains Supplementary Tables 1-4, Supplementary Figures 1-9, Supplementary Methods and Data, a Supplementary Discussion and Supplementary references. This file was replaced on 14 February 2013 to correct the DNA sequence in Supplementary Figure 8, which was misaligned.
- Supplementary Information 2 (244K)
This file contains the full formal specification of the digital information encoding scheme.
- Supplementary Information 3 (411K)
This file contains FastQC QC report on Illumina HiSeq 2000 sequencing run.
- Supplementary Data 1 (647K)
This zipped file contains the five original files encoded and decoded in this study, namely wssnt10.txt (ASCII text file containing text of all 154 Shakespeare sonnets), watsoncrick.pdf (PDF of Watson & Crick’s (1953) paper describing the structure of DNA), MLK_excerpt_VBR_45-85.mp3 (MP3 file containing a 26 s excerpt from Martin Luther King's 1963 "I Have A Dream" speech), EBI.jp2 (JPEG 2000 format medium resolution colour photograph of the European Bioinformatics Institute) and View_huff3.cd.new (ASCII text file defining the Huffman code used to convert bytes of encoded files to base 3).
- Supplementary Data 2 (6K)
This file contains the GATK ErrorRatePerCycle report on Illumina HiSeq 2000 sequencing run.