Abstract
Objective:
The aim of this study was to investigate relationships of cord blood cells in healthy term infants both from vaginal and Cesarean sections.
Study Design:
The study sample comprised 167 consecutive cord blood collections accepted for processing in an accredited cord blood bank. The effect of varying anticoagulant-to-blood ratio was excluded by standardizing the cell concentrations to reflect the values in native blood. Statistical analysis included descriptive statistics, simple linear regression analysis, Mann–Whitney U-test, cumulative frequency plots and Smirnov two-sample test.
Result:
As expected, hemoglobin correlated with red blood cell concentration. Interestingly, mean platelet volume was associated with hemoglobin, red blood cell concentration and hematocrit. The platelet count was inversely associated with the parameters.
Conclusion:
The observed associations of cord blood hemoglobin with mean platelet volume and platelet count reflect the physiology of fetal hematopoiesis at term.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gluckman E, Broxmeyer HA, Auerbach AD, Friedman HS, Douglas GW, Devergie A et al. Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical-cord blood from an HLA-identical sibling. N Engl J Med 1989; 321: 1174–1178.
Kurtzberg J, Laughlin M, Graham ML, Smith C, Olson JF, Halperin EC et al. Placental blood as a source of hematopoietic stem cells for transplantation into unrelated recipients. N Engl J Med 1996; 335: 157–166.
Rubinstein P, Carrier C, Scaradavou A, Kurtzberg J, Adamson J, Migliaccio AR et al. Outcomes among 562 recipients of placental-blood transplants from unrelated donors. N Engl J Med 1998; 339: 1565–1577.
Wagner JE, Barker JN, DeFor TE, Baker S, Blazar BR, Eide C et al. Transplantation of unrelated donor umbilical cord blood in 102 patients with malignant and nonmalignant diseases: influence of CD34 cell dose and HLA disparity on treatment-related mortality and survival. Blood 2002; 100: 1611–1618.
Aroviita P, Teramo K, Westman P, Hiilesmaa V, Kekomäki R . Associations among nucleated cell, CD34+ cell and colony-forming cell contents in cord blood units obtained through a standardized banking process. Vox Sang 2003; 84: 219–227.
Cairo MS, Wagner EL, Fraser J, Cohen G, van de Ven C, Carter SL et al. Characterization of banked umbilical cord blood hematopoietic progenitor cells and lymphocyte subsets and correlation with ethnicity, birth weight, sex, and type of delivery: a Cord Blood Transplantation (COBLT) Study report. Transfusion 2005; 45: 856–866.
Sparrow RL, Cauchi JA, Ramadi LT, Waugh CM, Kirkland MA . Influence of mode of birth and collection on WBC yields of umbilical cord blood units. Transfusion 2002; 42: 210–215.
Solves P, Moraga R, Saucedo E, Perales A, Soler MA, Larrea L et al. Comparison between two strategies for umbilical cord blood collection. Bone Marrow Transplant 2003; 31: 269–273.
Aroviita P, Teramo K, Hiilesmaa V, Westman P, Kekomaki R . Birthweight of full-term infants is associated with cord blood CD34+ cell concentration. Acta Paediatr 2004; 93: 1323–1329.
Orkin SH, Nathan DG, Ginsburg D, Look TA, Fisher DE, Lux SE (eds). Nathan and Oski's Hematology of Infancy and Childhood, 7th edn. Saunders Elsevier: Philadelphia, PA, 2009.
Christensen RD, Henry E, Jopling J, Wiedmeier SE . The CBC: reference ranges for neonates. Semin Perinatol 2009; 33: 3–11.
Eichler H, Seetharaman S, Latta M, Kurtz JW, Moroff G . Comparison of total nucleated cell measurements of UC blood samples using two hematology analyzers. Cytotherapy 2004; 6: 457–464.
Bourner G, Dhaliwal J, Sumner J . Performance evaluation of the latest fully automated hematology analyzers in a large, commercial laboratory setting: a 4-way, side-by-side study. Lab Hematol 2005; 11: 285–297.
Imeri F, Herklotz R, Risch L, Arbetsleitner C, Zerlauth M, Risch GM et al. Stability of hematological analytes depends on the hematology analyser used: A stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100. Clin Chim Acta 2008; 397: 68–71.
Ulich TR, del Castillo J, Senaldi G, Kinstler O, Yin S, Kaufman S et al. Systemic hematologic effects of PEG-rHuMGDF-induced megakaryocyte hyperplasia in mice. Blood 1996; 87: 5006–5015.
Eskola M, Rekunen S, Aroviita P, Mottonen S, Hiilesmaa V, Sainio S et al. Association of cord blood platelet characteristics and hematopoietic progenitor cells. Transfusion 2008; 48: 884–892.
Wilcox AJ, Skjaerven R . Birth weight and perinatal mortality: the effect of gestational age. Am J Public Health 1992; 82: 378–382.
Pihkala J, Hakala T, Voutilainen P, Raivio K . [Characteristics of recent fetal growth curves in Finland]. Duodecim 1989; 105: 1540–1546 (in Finnish).
Ballen KK, Wilson M, Wuu J, Ceredona AM, Hsieh C, Stewart FM et al. Bigger is better: maternal and neonatal predictors of hematopoietic potential of umbilical cord blood units. Bone Marrow Transplant 2001; 27: 7–14.
Schwartz R, Teramo KA . Effects of diabetic pregnancy on the fetus and newborn. Semin Perinatol 2000; 24: 120–135.
Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I . The ISHAGE guidelines for CD34+ cell determination by flow cytometry. J Hematother 1996; 5: 213–226.
Freise KJ, Schmidt RL, Gingerich EL, Veng-Pedersen P, Widness JA . The effect of anticoagulant, storage temperature and dilution on cord blood hematology parameters over time. Int J Lab Hematol 2009; 31: 496–504.
Larghero J, Rea D, Brossard Y, Van Nifterik J, Delasse V, Robert I et al. Prospective flow cytometric evaluation of nucleated red blood cells in cord blood units and relationship with nucleated and CD34+ cell quantification. Transfusion 2006; 46: 403–406.
Sainio S, Järvenpää AL, Renlund M, Riikonen S, Teramo K, Kekomäki R . Thrombocytopenia in term infants: a population-based study. Obstet Gynecol 2000; 95: 441–446.
Redzko S, Przepiesc J, Zak J, Urban J, Wysocka J . Influence of perinatal factors on hematological variables in umbilical cord blood. J Perinat Med 2005; 33: 42–45.
Wiedmeier SE, Henry E, Sola-Visner MC, Christensen RD . Platelet reference ranges for neonates, defined using data from over 47 000 patients in a multihospital healthcare system. J Perinatol 2009; 29: 130–136.
Mahaney MC, Brugnara C, Lease LR, Platt OS . Genetic influences on peripheral blood cell counts: a study in baboons. Blood 2005; 106: 1210–1214.
Acknowledgements
We thank all the participants of the CB bank program and the staff of the Finnish Cord Blood Bank. This work was funded by the Finnish Red Cross Blood Service Research Fund and the Nona and Kullervo Väre Foundation.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no conflict of interest.
Rights and permissions
About this article
Cite this article
Eskola, M., Juutistenaho, S., Aranko, K. et al. Association of cord blood platelet count and volume with hemoglobin in healthy term infants. J Perinatol 31, 258–262 (2011). https://doi.org/10.1038/jp.2010.95
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/jp.2010.95
Keywords
This article is cited by
-
A Physiologically-Based Pharmacokinetic Model to Predict Human Fetal Exposure for a Drug Metabolized by Several CYP450 Pathways
Clinical Pharmacokinetics (2017)