Data Descriptor | Open

An open access pilot freely sharing cancer genomic data from participants in Texas

Published online:


Genomic data sharing in cancer has been restricted to aggregate or controlled-access initiatives to protect the privacy of research participants. By limiting access to these data, it has been argued that the autonomy of individuals who decide to participate in data sharing efforts has been superseded and the utility of the data as research and educational tools reduced. In a pilot Open Access (OA) project from the CPRIT-funded Texas Cancer Research Biobank, many Texas cancer patients were willing to openly share genomic data from tumor and normal matched pair specimens. For the first time, genetic data from 7 human cancer cases with matched normal are freely available without requirement for data use agreements nor any major restriction except that end users cannot attempt to re-identify the participants (

Design Type(s)
  • parallel group design
  • disease state design
Measurement Type(s)
  • DNA sequence data
Technology Type(s)
  • Whole Exome Sequencing
  • Whole Genome Sequencing
Factor Type(s)
  • disease state
Sample Characteristic(s)
  • Homo sapiens
  • blood
  • head of pancreas
  • tail of pancreas
  • pancreas
  • inguinal lymph node

Background & Summary

New sequencing, data analysis, and visualization methodologies must be developed to realize the full potential of cancer genomic medicine as part of precision medicine1. Limited availability of accessible genomic reference datasets is a major bottleneck to maximizing this potential in a way that engages researchers and the public. It also hampers education in modern cancer bioinformatic analysis techniques using real world reference datasets. Most individual-level genomic data is currently accessible only through controlled-access initiatives (e.g., dbGaP, the database for genotypes and phenotypes; or cgHub, the cancer genomics hub), which require security and data-use agreements. Restricting access to individual-level genomic data protects against the risk of unauthorized re-identification, thereby reducing the possibility of discrimination against research participants and their families. However, patient identification is still possible in a controlled access database, whether intentional or not, and the broad policy of restricting access to these data inhibits research, as data in controlled access databases are not used as often as data in open access databases2. In addition, data use agreements not only limit who can access the data, placing a barrier between interested patients and genomic researchers3, but also where the data can be analysed. The latter hampers use of highly-accessible cloud-based platforms that have appropriate data control, access and accountability structures4.

Much of the ethical debate about data sharing has focused on the identifiability of genomic data and balancing privacy risks with the benefits of making data broadly available to the research community5,​6,​7,​8. There is an ethical responsibility to ensure not only that participants’ privacy is protected, but also that the utility of the data that they contribute for research is maximized2. In fact, limiting access to genomic data from participants who wish to share their data openly undermines their autonomy and may be viewed as unjustifiably paternalistic. We have previously argued that participants should be informed of both the risks and benefits of different levels of data sharing (i.e., controlled versus open access) and be allowed to make decisions about how broadly they are comfortable sharing their data8,9. A remaining concern lies in the notion that research participants may not be able to provide truly informed consent for genomic data sharing. Research participants often have poor recall of information presented in informed consent documents10,​11,​12 or with whom they had agreed to share their genomic data13. Additionally, obtaining consent from cancer patients in particular may be challenging due to protracted periods of psychological and physical stress associated with diagnosis and care14.

Here, we describe an OA project and its resulting genomic dataset demonstrating that many cancer patients could and would provide true informed consent (Fig. 1). Of these participants, seven were selected for OA release. Only a subset was released to protect privacy to the fullest extent possible. The TCRB provided a model for value-based collections15 in which 1) the highest possible data and specimen quality were maintained through standard consents, specimen and data collection protocols, pathological review procedures, material transfer agreements, and other control and documentation mechanisms that are freely available16; and 2) society can benefit through international sharing of genomic data through this OA effort and through TCRB’s contributions to the ICGC, the International Cancer Genome Consortium, and TCGA, the Cancer Genome Atlas17,18. As sequencing and data analytic technologies improve, the definition of quality must be broadened to tackle the next phase of cancer genomic research. TCGA accepted only the ‘ideal’ of frozen primary tumours with matched normal samples from participants who had not had prior treatment and whose samples met other stringent quality factors. A new frontier in cancer genomics research will be utilization of ‘real world’ cases—longitudinal studies of smaller specimens such as biopsies; multiple biopsies of a single tumor to better model heterogeneity; and evaluation of tumours from patients who have had prior antineoplastic interventions or whose samples do not meet TCGA’s rigorous pathological quality control standards. Some such specimens are included in this dataset to present an opportunity for the community to develop methods to better utilize data from these types of cases, in an attempt to maximize benefit to cancer patients, many of whose samples will never meet ‘ideal’ conditions for genetic research.

Figure 1: The Texas Cancer Research Biobank (TCRB) Open Access (OA) data sharing workflow.
Figure 1

Participants provided initial consent to participate in OA data sharing. Those who granted consent were educated on risks and benefits then quizzed to test understanding and asked whether they wanted to opt out of OA sharing. Tumor and normal blood specimens from those who showed comprehension and reaffirmed consent were then obtained and subjected to whole exome sequencing.

Efforts such as the Personal Genome Project in the US, Canada, UK and Austria are publicly sharing genomic and clinical data from healthy participants19. To our knowledge, the TCRB OA dataset is the first individual-level, open-access genomic data release targeted to human cancers. Lowering barriers to access increases potential for misuse of the data, but with appropriate informed consent and reasonable steps taken to protect patient confidentiality, this dataset confirms that many patients recognize that positive future benefits can outweigh these risks. This consent coupled with public access to a large pool of readily-available data is critical to addressing particularly difficult challenges with human cancers.


Obtaining informed consent

All work was carried out as part of an IRB-approved protocol (BCM IRB H-32711), which utilized a main consent document for general participation in TCRB with an opt-in consent addendum for OA data release. Of 194 TCRB participants offered the option of signing the opt-in addendum participating in OA sharing out of >2,500 total participants, more than half agreed to open access data sharing at time of consent. Annotated TCRB specimen and data collection consent and the OA opt in consent documents are available at To address concerns about whether patients can provide truly informed consent regarding the potential risks of genomic data sharing, a subset of the OA participants (n=37) were educated on risks and societal benefits of data sharing. The educational materials are available at Participants were surveyed to assess their comprehension, risk tolerance, and subjective comfort with OA data release. Each participant was again queried, post-survey, to reconfirm their choice to take part in the OA data sharing option. The majority demonstrated adequate understanding of the possible privacy and discrimination risks, yet still elected to allow their data to be openly shared. The work described in Pereira et al. 9 is one clear example that many, though not all, cancer patients indeed desire to participate in activities that could have broad-reaching, positive impacts to public health for reducing cancer mortality and morbidity, and have the capability to make an informed choice.

Selecting the OA cohort

Approximately 20% of the 37 participants (n=7) who were surveyed and still consented to OA data sharing were selected for inclusion in the TCRB cancer OA dataset. To further reduce risk of reidentification, none of the OA participants had rare ethnicities or tumor types as defined by SEER (Surveillance, Epidemiology, and End Results program) statistics. Figure 1 shows the process for this and subsequent steps in creation of this dataset.

Tumor and normal collection

Blood was collected from participants in PAXgene Blood DNA tubes, and DNA was isolated using the PAXgene Blood DNA kit (PreAnalytiX, Qiagen, Valencia, CA). The tumor pancreas tissue specimens were collected shortly after resection and stored in a protease inhibitor solution (Roche Applied Science, Indianapolis, IN), RNAlater (Qiagen), or snap frozen, and stored at −80C. The blood sample specimen was used as matching normal control. DNA was isolated from 50–100 mg tissue fragments using the GentraPuregene kit (Qiagen). The quality of the DNA samples were ascertained by electrophoresis and determined to be of high quality (size >23 kb) with no visible degradation in blood or tumor samples.

To implement genomic sequencing approaches in ‘real world’ specimens, it is imperative to detect variants in clinical samples that have reduced tumour cellularity, e.g., as a consequence of neoadjuvant or other prior therapy. We devised methodologies to overcome the challenges associated with extensive desmoplastic stroma that is characteristic of the majority of pancreatic tumours, and these strategies facilitated the discovery of novel molecular mechanisms in the pathophysiology of this disease. The cellularity of each primary sample was estimated through pathological review, deep amplicon-based sequencing of exons 2 and 3 of KRAS (average depth of 1,000×), and single nucleotide polymorphism (SNP) array-based cellularity estimates using a novel algorithm qpure20. Clinical and pathological annotations for each case are shown in Table 1.

Table 1: TCRB open access clinical and pathological annotations by case.

Whole exome sequencing

Library preparation

DNA samples were constructed into Illumina paired-end pre-capture libraries according to the manufacturer’s protocol (Illumina Multiplexing_SamplePrep_Guide_1005361_D) with modifications as described in the BCM-HGSC Illumina Barcoded Paired-End Capture Library Preparation protocol. Libraries were prepared using Beckman robotic NXp and FXp model workstations. The complete protocol and oligonucleotide sequences are accessible from the HGSC website ( Briefly, 1 ug of DNA in 100 ul volume was sheared into fragments of approximately 300–400 base pairs in a Covaris plate with E210 system (Covaris, Inc. Woburn, MA) followed by end-repair, A-tailing and ligation of the Illumina multiplexing PE adaptors. Pre-capture Ligation Mediated-PCR (LM-PCR) was performed for 7 cycles of amplification using the Phusion High-Fidelity PCR Master Mix (NEB, Cat. no. M0531L). Universal primer IMUX-P1.0 and a pre-capture barcoded primer IBC were used in the PCR amplification. In total, a set of 12 such barcoded primers were used on these samples. Purification was performed with Agencourt AMPure XP beads after enzymatic reactions. Following the final XP beads purification, quantification and size distribution of the pre-capture LM-PCR product was determined using the LabChip GX electrophoresis system (PerkinElmer).

Exome capture

For exome capture, four pre-capture libraries were pooled together (~300 ng/sample, 1.2 ug/pool) and hybridized in solution using the VCRome 2.1 Design21 supplied by NimbleGen according to the manufacturer’s protocol NimbleGen SeqCap EZ Exome Library SR User’s Guide (Version 2.2) with minor revisions. Human COT1 DNA and full-length Illumina adaptor-specific blocking oligonucleotides were added into the hybridization to block repetitive genomic sequences and the adaptor sequences. Post-capture LM-PCR amplification was performed using the Phusion High-Fidelity PCR Master Mix with 14 cycles of amplification. After the final AMPure XP bead purification, quantity and size of the capture library was analyzed using the Agilent Bioanalyzer 2100 DNA Chip 7500. The efficiency of the capture was evaluated by performing a qPCR-based quality check on the four standard NimbleGen internal controls. Successful enrichment of the capture libraries was estimated to range from a 6 to 9 of ΔCt value over the non-enriched samples.


Library templates were prepared for sequencing using Illumina’s cBot cluster generation system with TruSeq PE Cluster Generation Kits (Cat. no. PE-401–3001). Briefly, these libraries were denatured with sodium hydroxide and diluted to 6–9 pM in hybridization buffer in order to achieve a load density of ~800 K clusters/mm2. Sequencing runs were performed in paired-end mode using the Illumina HiSeq 2000 platform. Each library pool was loaded in a single lane of a HiSeq 2000 flow cell, and each lane was spiked with 2% phiX control library for run quality control. The sample libraries then underwent bridge amplification to form clonal clusters, followed by hybridization with the sequencing primer. Using the TruSeq SBS Kits (Cat. no. FC-401–3001), sequencing-by-synthesis reactions were extended for 101 cycles from each end, with an additional 7 cycles for the index read. Sequencing runs generated approximately 300–400 million successful reads on each lane of a flow cell, yielding 7–13 Gb per sample. For exome sequencing yields, samples achieved an average of depth of coverage of 200X over exonic regions.

Whole genome sequencing

Library preparation

For most cases, there was not sufficient quantities of biospecimen available to perform whole genome sequencing (WGS). For these cases, only WEX data exist. For two cases, however, it was possible to perform WGS. Library templates were prepared for sequencing using Illumina’s cBot cluster generation system with TruSeq PE Cluster Generation Kits (Cat. no. PE-401–3001). DNA (0.5 ug) in 70 ul volume was sheared into fragments of approximately 500–700 base pairs using Covaris S2 system (Covaris, Inc. Woburn, MA). Briefly, these libraries were denatured with sodium hydroxide and diluted to 6–9 pM in hybridization buffer in order to achieve a load density of ~800 K clusters/mm2. Illumina multiplexing PE adaptors with barcode sequences were added to the sample at the time of ligation. Pre-capture Ligation Mediated-PCR (LM-PCR) was performed for 6–8 cycles using the Library Amplification Readymix containing Kapa HiFi DNA Polymerase (Kapa Biosystems, Inc., Cat # KK2612) and universal primer-pair IMUX-P1.0 and IMUX-P3.0. 4) For purification of the fragmented DNA, 0.8X AMPure XP (Beckman, Cat. No. A63882) was used as opposed to using 1.8X for preparation of WES libraries.


Tumor libraries were sequenced in four lanes, and normal libraries were sequenced in two lanes of a HiSeq 2000 flow cell, yielding approximately 60X and 30X coverage respectively. Each lane was spiked with 2% phiX control library for run quality control. The sample libraries then underwent bridge amplification to form clonal clusters, followed by hybridization with the sequencing primer. Sequencing runs were performed in paired-end mode using the Illumina HiSeq 2000 platform. Using the TruSeq SBS Kits (Cat. no. FC-401–3001), sequencing-by-synthesis reactions were extended for 101 cycles from each end, with an additional 7 cycles for the index read. Sequencing runs generated approximately 300–400 million successful reads on each lane of a flow cell, yielding ~11 Gb per sample.

Read mapping and variant calling

Binary bcl files output from the HiSeq 2000 were processed with BclConvertor 1.7.1 software available from Illumina. All reads from the prepared libraries that passed the Illumina Chastity filter were formatted into FASTQ files, which were aligned to the human reference genome build 19 version GRCh37-lite using BWA 0.5.9rcl (Burrows-Wheeler Aligner)22. Default parameters were used for alignment, except for a 40 bp seed sequence with 2 mismatches in the seed and a total of 3 mismatches overall allowed. Subsequent base quality recalibration and local realignment around known indel sites was performed by GATK 3.3 (ref. 23). Variant calling was performed using on samples using Atlas-SNP2 (ref. 24), Atlas-Indel2 (ref. 25), and PInDel26. Variants from tumor and matched normal pair samples were compared with each other and with NCBI human reference genome version 19 (hg19) to generate primary calls, which then were annotated using Annovar27, COSMIC28, and dbSNP29 and split into germline or somatic calls in VCF files. The germline and somatic calls were filtered by curators for quality and are available in MAF files. FASTQ, BAM, VCF and filtered MAF and available clinical annotations are freely available on the TCRB website (Data Citation 1: TCRB Open Access Repository TCRBOA1) and FASTA and BAMs also within SRA, the Sequence Read Archive (Data Citation 2: NCBI Sequence Read Archive PRJNA284596 and Data Citation 3: NCBI Sequence Read Archive PRJNA284598).

Code availability

All software used to generate the sequence data and to manage biospecimen and clinical annotations is freely available. Specific software versions and references for code are provided as in-line references above.

Data Records

FASTQ reads and BAM data records for tumor (T) and normal (N) specimens from each case are freely available along with their conditions of use are freely available on the Texas Cancer Research Biobank website, (Data Citation 1: TCRB Open Access Repository TCRBOA1). Clinical annotations available for these cases are defined in Table 1. Other than a click-through agreement to acknowledge the conditions of use, requirement to create an access account for auditing purposes, and include these conditions within any re-sharing of the data, there are no additional barriers to data access on this portal. User accounts are valid for 30 days and can be renewed. All or some of these data may be downloaded, shared and redistributed for research and educational purposes in accordance with their conditions of use.

To ensure sustainable availability of the data, they are also deposited within SRA. We created the Texas Cancer Research Biobank Open Access Data Sharing Umbrella Project (Accession: PRJNA285925) under which two platform-specific projects were created—the subproject entitled the Texas Cancer Research Biobank Open Access Data Sharing: Exome Project (Data Citation 2: NCBI Sequence Read Archive PRJNA284596) that includes all seven cases and the subproject entitled the Texas Cancer Research Biobank Open Access Data Sharing: Genome Project (Data Citation 3: NCBI Sequence Read Archive PRJNA284598), for which sufficient genetic material remained for whole genome sequencing after whole exome sequencing was performed and includes cases 6 and 7.

The NCI and others are considering mechanisms to bring the computational capabilities to the data to circumvent issues with transferring large files over networks. For end users who lack sufficient local computation and/or storage capabilities or for whom data downloads may be challenging, a third copy of the data records, their conditions of use, and the HGSC’s Mercury informatics pipeline is available on the DNAnexus cloud. The click-through conditions of use and data can be accessed at

Additional data records such as whole genome sequences may be generated from these cases, they will be added to each of these repositories.

Technical Validation

The TCRB utilized a secure, database-backed web application called Acquire30 (code available at for tracking specimens and their annotations. Through its modules, it supports the full lifecycle of biobanking operations, from collections to quality control testing. Public researchers can use the specimen request module to electronically search for and request available specimens. Acquire greatly facilitated non-OA TCRB donations to the TCGA and ICGC.

As TCRB tissue advocates at sites across the state of Texas consented patients, collected specimens, and entered data into Acquire. The system automatically assigns a barcode and UUID (universally unique identifier) to each specimen, aliquot and derivative. These identifiers are completely masked and contain no PHI or other data that can be mapped back to participants, such that the system’s administrators held the mapping for the UUIDs to participant identifiers acted as the TCRB honest broker. All specimens underwent initial review by expert pathologists for disease diagnosis at the Texas hospital or clinic at which the patients were consented. The TCRB’s own expert pathologists reviewed slides cut from the tumor specimens to validate the initial diagnosis and other pathological characteristics such as percent cellularity and necrosis according to TCRB pathology procedures. When minimal data entry was complete, tumor and normal matched pairs were added to shipment reports in Acquire and the data were transmitted to HGSC’s laboratory information management system (LIMS). These reports use only masked specimen identifiers from Acquire. A copy of the shipment report was also printed and included with the specimens to provide a shipping manifest as a secondary identification measure. Specimen barcodes were scanned to confirm the identity of the tumor and normal samples and cross-referenced against the list of expected barcodes. Verified specimens were given a de-identified HGSC accession number for storage in their LIMS. HGSC personnel isolated nucleic acids and obtained quality measurements such as total nucleic acid concentration, RIN values for RNA or polyacrylamide gels for DNA. These metrics were saved to HGSC’s LIMS and also entered into Acquire as annotations on the specimen. Consent and specimen collection forms as well as procedures for specimen collection and shipping are available at

Research coordinators re-examined the medical records of each OA participant relative to the data entered into Acquire to assure that clinical annotations were correct. The Acquire coordinator compiled clinical and pathological annotations with HGSC accession numbers and other data for each case.

To verify that the resultant BAM files were not corrupted and could be re-processed from FASTQ files in another environment, the BCM files were re-processed using DNAnexus cloud-based instance of the HGSC’s Mercury pipeline31, pertinent algorithms in which are described within the Methods section. All passed this edit check.

Quality analyses were performed on WEX and WGS data, and all data files fall within acceptable parameters as shown in Table 2. All samples were of adequate quality for variant analysis as determined by concordance and contamination estimates based on SNP array (see Table 2). We also compared the ratio of novel variants to known high-quality dbSNP variants detected from our somatic variant analysis and found that all samples were within acceptable limits, no abundance of population variation was noted in the somatic calls indicating that the samples are properly paired and show no detectable contamination from other samples above background. Allele fraction analysis, comparing the ratio of variant reads to total reads, of the somatic variants indicated that most of the samples were of low tumor cellularity, generally less than 20% (Fig. 2) with the exception of TCRBOA7 which has an estimated allele fraction peak of 25% and an estimated tumor cellularity of 50%. These features make this cohort quite useful for algorithm developers seeking to tune their callers to low tumor cellularity samples. Table 3 summarizes the SNP and indel counts, driver mutations uncovered in each case and a comparison of the percent cellularity as measured by driver variant allelic fraction and by expert pathologists. In all cases where data were available, pathological cellularity determinations were higher than those by allelic fraction.

Table 2: Quality control data for whole exome (WEX) and whole genome (WGS) sequencing strategies by case.
Figure 2: Allele fraction density plot for all TCRB-OA samples.
Figure 2

Variants were restricted to the coding region so that WEX and WGS samples are comparable. Allele fraction (ratio of variant reads to total reads) density is plotted for each sample.

Table 3: Summary of filtered somatic variants from MAF files of whole exome (WEX) and whole genome (WGS) data by case.

Usage Notes

By downloading or utilizing any part of this dataset, end users must agree to the following conditions of use:

  • No attempt to identify any specific individual represented by these data or any derivatives of these data will be made.

  • No attempt will be made to compare and/or link this public data set or derivatives in part or in whole to private health information.

  • These data in part or in whole may be freely downloaded, used in analyses and repackaged in databases.

  • Redistribution of any part of these data or any material derived from the data will include a copy of this notice.

  • The data are intended for use as learning and/or research tools only.

  • This data set is not intended for direct profit of anyone who receives it and may not be resold.

  • Users are free to use the data in scientific publications if the providers of the data (Texas Cancer Research Biobank and Baylor College of Medicine Human Genome Sequencing Center) are properly acknowledged.

Implementation of common vocabularies facilitates semantic interoperability and data reuse. Annotations for OA cases include controlled terminologies for race, gender and ethnicity from the NIH; pathological diagnosis from World Health Organisation’s ICD-O-3 (International Classification of Diseases-Oncology version 3); and tumor stage and grade data from Union for International Cancer Control (UICC/AJCC). Use of standard metadata facilitates syntactic interoperability. The TCRB used standard data elements, file formats and metadata. Because OA data can be downloaded and re-shared on the provision that the conditions of use are included and abided by, all annotations were also included as comments in BAM file headers with a reference to the conditions of use. These metadata were included to ensure that the clinical and pathological data cannot become decoupled from the sequence data.

Additional Information

How to cite this article: Becnel, L. B. et al. An open access pilot freely sharing cancer genomic data from participants in Texas. Sci. Data 3:160010 doi: 10.1038/sdata.2016.10 (2016).


  1. 1.

    , & National Human Genome Research, I. Charting a course for genomic medicine from base pairs to bedside. Nature 470, 204–213 (2011).

  2. 2.

    , , & Research ethics. The complexities of genomic identifiability. Science 339, 275–276 (2013).

  3. 3.

    et al. Detection of a recurrent DNAJB1-PRKACA chimeric transcript in fibrolamellar hepatocellular carcinoma. Science 343, 1010–1014 (2014).

  4. 4.

    et al. Genomic cloud computing: legal and ethical points to consider. Eur. J. Hum. Genet. 23, 1271–1278 (2014).

  5. 5.

    , , , & Identifying personal genomes by surname inference. Science 339, 321–324 (2013).

  6. 6.

    et al. Resolving individuals contributing trace amounts of DNA to highly complex mixtures using high-density SNP genotyping microarrays. PLoS genetics 4, e1000167 (2008).

  7. 7.

    , & Genetics. Genomic research and human subject privacy. Science 305, 183 (2004).

  8. 8.

    & Genetics. No longer de-identified. Science 312, 370–371 (2006).

  9. 9.

    , & Open access data sharing in genomic research. Genes (Basel) 5, 739–747 (2014).

  10. 10.

    , , & Informed consent: How much does the patient understand? Clin. Pharmacol. Ther. 27, 435–440 (1980).

  11. 11.

    , , , & Quality of informed consent in cancer clinical trials: A cross-sectional survey. Lancet 358, 1772–1777 (2001).

  12. 12.

    , & Are research subjects really informed? West. J. Med. 123, 76–80 (1975).

  13. 13.

    , , , & Participants' recall and understanding of genomic research and large-scale data sharing. J. Emp. Res. Human Res. Eth. 8, 42–52 (2013).

  14. 14.

    et al. The impact of disease severity on the informed consent process in clinical research. Am. J. Med. 100, 261–268 (1996).

  15. 15.

    & Biobanking 3.0: evidence based and customer focused biobanking. Clin Biochem. 47, 300–308 (2014).

  16. 16.

    The Texas Cancer Research Biobank webpage. Resources page . Retrieved on 01/08/2016.

  17. 17.

    et al. Pancreatic cancer genomes reveal aberrations in axon guidance pathway genes. Nature 491, 399–405 (2012).

  18. 18.

    Cancer Genome Atlas Research, N. Comprehensive molecular characterization of clear cell renal cell carcinoma. Nature 499, 43–49 (2013).

  19. 19.

    et al. A public resource facilitating clinical use of genomes. Proc. Natl. Acad. Sci. USA 109, 11920–11927 (2012).

  20. 20.

    et al. qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles. PLoS ONE 7, e45835 (2012).

  21. 21.

    et al. Targeted enrichment beyond the consensus coding DNA sequence exome reveals exons with higher variant densities. Genome Biol. 12, R68 (2011).

  22. 22.

    The Burrows-Wheeler development team. Burrows-Wheeler Aligner SourceForge Page Retrieved on 12/22/2015.

  23. 23.

    The Broad Institute. GATK Version Archive . Retrieved on 12/22/2015.

  24. 24.

    et al. A SNP discovery method to assess variant allele probability from next-generation resequencing data. Genome Res 20, 273–280 (2010).

  25. 25.

    et al. An integrative variant analysis suite for whole exome next-generation sequencing data. BMC Bioinformatics 13, 8 (2012).

  26. 26.

    , , , & Pindel: a pattern growth approach to detect break points of large deletions and medium sized insertions from paired-end short reads. Bioinformatics 25, 2865–2871 (2009).

  27. 27.

    , & ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res. 38, e164 (2010).

  28. 28.

    et al. COSMIC: exploring the world's knowledge of somatic mutations in human cancer. Nucleic Acids Res. 43, D805–D811 (2015).

  29. 29.

    et al. dbSNP: the NCBI database of genetic variation. Nucleic acids research 29, 308–311 (2001).

  30. 30.

    et al. Acquire: an open-source comprehensive cancer biobanking system. Bioinformatics 31, 1655–1662 (2015).

  31. 31.

    et al. Launching genomics into the cloud: deployment of Mercury, a next generation sequence analysis pipeline. BMC Bioinformatics 15, 30 (2014).

Download references

Data Citations

  1. 1.

    Becnel, I. TCRB Open Access Repository (2015).

  2. 2.

    Becnel, I. NCBI Sequence Read Archive (2015).

  3. 3.

    Becnel, I. NCBI Sequence Read Archive (2015).


We thank the patients and their families who participated in the Texas Cancer Research Biobank, Heidi Dowst and other members of the Texas Cancer Research Biobank, Divya Kalru and Walker Hale for assistance in submitting data to the SRA, Gerald Wright for assistance with the TCRB website and Narayanan Veeraraghavan, Andrew Carroll, Mike Lin and Singer Ma for revalidating with the Mercury pipeline the TCRB OA FASTQ files on the DNAnexus cloud and hosting the FASTQ and BAM files in their cloudspace. This work was funded by the Cancer Prevention Research in Texas (CPRIT) through a multi-investigator research award RP101353, with some additional support from the NHGRI as part of its Genome Center program, U54 HG003273, and the NCI/NIH as part of its Cancer Center Support Grant program, P30 CA125123.

Author information


  1. Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, Texas 77030, USA

    • Lauren B. Becnel
  2. Center for Medical Ethics and Health Policy, Baylor College of Medicine, Houston, Texas 77030, USA

    • Stacey Pereira
    •  & Amy L. McGuire
  3. Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas 77030, USA

    • Jennifer A. Drummond
    • , Marie-Claude Gingras
    • , Kyle R. Covington
    • , Christie L. Kovar
    • , Harsha Vardhan Doddapaneni
    • , Jianhong Hu
    • , Donna Muzny
    • , David A. Wheeler
    •  & Richard A. Gibbs


  1. Search for Lauren B. Becnel in:

  2. Search for Stacey Pereira in:

  3. Search for Jennifer A. Drummond in:

  4. Search for Marie-Claude Gingras in:

  5. Search for Kyle R. Covington in:

  6. Search for Christie L. Kovar in:

  7. Search for Harsha Vardhan Doddapaneni in:

  8. Search for Jianhong Hu in:

  9. Search for Donna Muzny in:

  10. Search for Amy L. McGuire in:

  11. Search for David A. Wheeler in:

  12. Search for Richard A. Gibbs in:


L.B. provided project management, oversaw all biomedical informatics and drafted the manuscript. S.P. co-wrote T.C.R.B. consents, co-created O.A. participant educational and survey materials, administered surveys of risk/benefit assessment to participants, evaluated scores, and edited the manuscript. J.D. and K.R.C. performed bioinformatics analysis on all cases and edited the manuscript. D.M. oversaw production sequencing pipelines with C.K. and H.D. assisting on W.G.S. and J.H. on W.E.X. M.C.G. managed library preparation, exome capture and sequencing and edited manuscript text describing these activities. A.M. provided ethical, legal and social implications expertise and co-wrote T.C.R.B. consents, and O.A. participant educational and survey materials. D.W. oversaw all cancer bioinformatics work on this project. R.G. oversaw all aspects of this project and edited the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Lauren B. Becnel.

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit Metadata associated with this Data Descriptor is available at and is released under the CC0 waiver to maximize reuse.