Abstract
Despite the wide knowledge about prevalent effects of ocean acidification on single species, the consequences on species interactions that may promote or prevent habitat shifts are still poorly understood. Using natural CO2 vents, we investigated changes in a key tri-trophic chain embedded within all its natural complexity in seagrass systems. We found that seagrass habitats remain stable at vents despite the changes in their tri-trophic components. Under high pCO2, the feeding of a key herbivore (sea urchin) on a less palatable seagrass and its associated epiphytes decreased, whereas the feeding on higher-palatable green algae increased. We also observed a doubled density of a predatory wrasse under acidified conditions. Bottom-up CO2 effects interact with top-down control by predators to maintain the abundance of sea urchin populations under ambient and acidified conditions. The weakened urchin herbivory on a seagrass that was subjected to an intense fish herbivory at vents compensates the overall herbivory pressure on the habitat-forming seagrass. Overall plasticity of the studied system components may contribute to prevent habitat loss and to stabilize the system under acidified conditions. Thus, preserving the network of species interactions in seagrass ecosystems may help to minimize the impacts of ocean acidification in near-future oceans.
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Introduction
Ecological systems are organized in more or less complex networks of species interactions; being the strength of such interactions critical on how the system is structured and respond to environmental change1,2,3. Ocean acidification (OA) due to increasing human emissions of carbon dioxide (CO2) into the atmosphere is causing unequal positive or negative impacts on different species4,5, thus changing the strength of their interactions with largely unpredictable effects on ecosystems6.
Of particular concern are OA-driven changes on foundation (habitat-forming) species, since they may modify habitat structural complexity and the environmental context in which other species interact. Most studies on kelp forests and coral reefs showed major shifts to simplified habitats dominated by turf-algae7,8. A boosted primary productivity of certain non-calcifying species, which are able to benefit from both the increase in inorganic carbon availability for photosynthesis and the reduction in the abundance of dominant calcareous producers, is consistently reported9,10,11. However, reports are not uniform regarding the control exerted by consumers on the bloomed productivity. Some experiments8 and observations at CO2 vents12 indicate that under acidified conditions the enhanced resource effect of high pCO2 on turf algae, combined with a weakened top-down control by herbivores (reduced abundance and/or feeding), drive the reorganization of kelp forests towards turf-dominated systems. Other mesocosm experiments suggest that an amplified herbivory control on the boosted productivity may alternatively stabilize the system13,14. In both cases, herbivory plays a central role in promoting the system resistance or change since it acts as connector of bottom-up (resource-driven) and top-down processes (consumer-driven).
In iconic seagrass systems, shifts to algal-dominated habitats are often associated to a reduced grazing by small invertebrate herbivores on epiphytes and opportunistic algae, whilst shifts to unvegetated habitats are associated to extremely high herbivory events by sea turtles or sea urchins (review by Maxwell et al.15). Such shifts are largely due to eutrophication and/or the removal of predators that control herbivore populations by overfishing, either directly or indirectly via reduced control on small predators that feed on algae-removing mesograzers. We know comparatively less about changes in the strength of species interactions that may critically influence the persistence of seagrass ecosystems under ocean acidification.
The Mediterranean endemic seagrass Posidonia oceanica forms complex systems with well-defined main trophic links. Two macroherbivores alone, the sea urchin Paracentrotus lividus and the sparid fish Sarpa salpa (commonly known as salema), may remove 50% of the annual seagrass productivity in shallow meadows16. In this study, we investigate mechanisms behind the change or stability of Posidonia habitats under ocean acidification. Particularly, we examined the interactions’ strength under present (off-vent) and near-future OA conditions (CO2 vents) of a tri-trophic food chain embedded within all its natural complexity. We studied multiple basal resources, one of the two main seagrass herbivores (sea urchin), and a territorial labrid fish that is known to predate on such herbivore17,18 (Symphodus tinca, commonly known as peacock wrasse). Both consumers have restricted benthic home ranges, which ensures long-term exposure to high pCO2 levels at the vent sites for them and their resources. Particularly, we investigated the strength of consumers’ feeding by quantifying CNP stoichiometry, diet composition, trophic niche and position (stable isotope analysis (SIA)-based and diet-based), as well as the availability and palatability of resources to herbivores. We also examined the strength of the predation pressure mediated by both, direct (predator abundance) and indirect interactions (habitat structure influencing refuge provision and fish herbivory as modifier of such structure). Herbivore abundance, resulting from the propagation of both, resource- and predator-driven effects, was also quantified.
The most recent experiments on OA research (e.g. refs. 8,19) incorporate a few species interactions and a longer exposure to CO2 enrichment than the conventional experiments, which are typically restricted to short-term exposure of a single species or trophic level. Still, laboratory or mesocosm experiments cannot reflect the full complexity of the interactions’ network, nor the full compensatory and adaptive potential of species gained across generations. On the contrary, the use of natural CO2 vents to replicate future OA effects on ecosystems overcomes such experimental constraints, in spite of some limitations such as the relatively small spatial scales and open nature of vent systems.
Results
Herbivore feeding: food abundance and quality
We detected no differences between sites in the niche width or in the number of carbon resources exploited by sea urchin populations, which indeed showed similar Standard Ellipse Areas and bootstrapped Layman metrics (Fig. 1a). In contrast, the isotopic niche centroid of sea urchins differed among sites (PERMANOVA Pseudo-F = 26, p = 0.001), occupying the most δ15N depleted and δ13C enriched position at off-vent followed by south-vent and with north-vent occupying the opposite position (Fig. 1, PERMANOVA p = 0.0001 for resource x site interaction in both, δ15N and δ13C). No difference in sea urchin stoichiometry was found between sites (Fig. 2a; PERMANOVA non-significant p-values for CNP contents and ratios).
Diet-based trophic position of sea urchins was lower at vents than at off-vent (Fig. 2b; PERMANOVA Pseudo-F = 8.8, p = 0.001) due to a lower ingestion of seagrass and calcareous epiphytes (50–57% at vents vs. 84% at off-vent) at both vents, where the green algae Flabellia and Cladophora were more consumed (ca. 25% at vents versus 3% at off-vent; see Fig. 3, diet composition PERMANOVA Pseudo-F = 6.9, p = 0.0001; pairwise comparisons: off-vent ≠ north- ~ south-vent). Using the SIA-based method, a lower trophic position of sea urchins was found only at north-vent than at off-vent (PERMANOVA Pseudo-F = 3.8, p = 0.03). This reflects a lower detection ability of the SIA method likely related to the appropriate choice of baseline references, which is particularly tricky for herbivores since basal resources have a high interspecific isotopic variability20. These changes in diet agreed with the resource palatability that we observed in feeding assays, as sea urchins preferred off-vent Posidonia and vent Flabellia (Fig. 4). The lower preference for vent Posidonia was likely related to its higher leaf thickness and cross-sectional area (Fig. 5c), since the seagrass nutritional and chemical traits that we measured did not consistently vary between vent and off-vent sites (Fig. 5a,b). The preference for off-vent Posidonia disappeared when epiphytes were present in seagrass leaves. Seagrass epiphytes showed higher nutritional quality at both vents than off-vent (higher N:P and C, N and P content, and lower C:N; higher sucrose and starch as follows south-> north-> off-vent), but also higher phenolics. Among algal resources with an amplified contribution to urchin diet at vents, Cladophora’s nutritional quality was higher at both vents than off-vent (higher N, N:P, sucrose and starch; Fig. 5). Urchin preference on other algae was not related to the food quality traits that we measured, i.e. the sea urchins preference for vent Flabellia with lower P content and higher C:P and N:P; for vent Halopteris with higher sucrose, N and P contents, but also higher C and phenolics; for off-vent Peyssonnelia with lower N content; and no preference for vent Jania with higher N and P contents and lower C:N and C:P. Calcification degree significantly varied between calcareous resources (Jania > Epiphytes > Peyssonnelia; PERMANOVA p = 0.0001 for resource), with a tendency (PERMANOVA p = 0.05 for site) to lowered calcification at vents (Fig. 5c).
The diversity of food resources available to herbivores, as well as the abundance of the common algae Flabellia, Halopteris and Peyssonnelia, was similar at vents and off-vent (Table 1). Posidonia was more abundant at both vents, where it had less epiphytes, particularly at south-vent. Cladophora was scarce at off-vent and slightly more abundant at both vents. Jania was very scarce at the south-vent (found in sea urchin guts but not in the meadow), while at north-vent and off-vent it was also found as epiphyte of Flabellia and Halopteris. Asparagopsis was particularly abundant in shallow areas at the north-vent and absent at south-vent, whereas only the tetrasporophyte (“Falkenbergia-phase”) of this alga was found off-vent. Other scarce algae, which also appeared in sea urchin’s diet, were Cladophora spp. and Dictyota spp., with the later absent off-vent. The green algae Caulerpa cylindracea (all sites), Anadyomene stellata (off-vent, south-vent), and Bryopsis spp (off-vent, north-vent) were very scarce in the meadows and absent in consumers’ diet.
Predator feeding
We observed a relatively higher degree of omnivory (NR) and similitude in trophic habits of wrasses (SDNND), as well as lower diversity of carbon resources exploited (CR), at south-vent than off-vent; but these were single values not tested for significance (Fig. 1). The position of the niche centroid of wrasse populations did not significantly differ between vents and off-vent (Fig. 1a; PERMANOVA Pseudo-F = 3.6, p = 0.09), despite the depleted δ13C values found at the off-vent (Fig. 1b; PERMANOVA Pseudo-F = 22, p = 0.01). Wrasse stoichiometry did not vary between sites, with the exception of a higher N:P ratio at the south-vent than off-vent (Fig. 2a; PERMANOVA Pseudo-F = 13, p = 0.02). The SIA-based trophic position of wrasses was higher at off-vent than at south-vent (PERMANOVA Pseudo-F = 37, p = 0.004), while the diet-based trophic position did not significantly vary between sites (Fig. 2b; PERMANOVA Pseudo-F = 0.001, p = 0.98). Site-dissimilarity in wrasse diet was also relatively high (58%; mainly due to a higher ingestion of gastropods and amphipods at south-vent instead of foraminifera, isopods, sipunculid worms, and bivalves at off-vent, see Fig. 3), but differences were not significant due to a high variability among the low number of replicates (diet composition PERMANOVA Pseudo-F = 2.0, p = 0.15).
Herbivore and predator abundance
We found no differences between vents and off-vent in sea urchin abundance (Fig. 6a). Regardless of the site, sea urchins were more abundant than wrasses, while predator abundance was significantly higher at the south-vent with a more than doubled density than off-vent (mean ± SD: 0.03 ± 0.03 and 0.01 ± 0.03 individuals m−2, respectively; PERMANOVA consumer x site interaction Pseudo-F = 2.7, p = 0.03). Albeit not statistically significant due to a high variability in wrasse distribution, the same trend with even a higher mean (0.06 ± 0.11 individuals m−2) was observed at the north-vent (Fig. 6a).
Fish herbivory, habitat structure and refuge provision
Seagrass shoots at both vent sites showed clear signs of intense fish herbivory, with leaves in each shoot much more eaten by salemas than at off-vent (35 ± 43% bitten leaves at south-vent and 22 ± 32% at north-vent versus 4 ± 9% at off-vent; see Fig. 6b, PERMANOVA Pseudo-F = 4.1, p = 0.01). This intense fish herbivory resulted in a significantly lower canopy height at both vents (Fig. 6c; PERMANOVA Pseudo-F = 3.1, p = 0.0498), where the rhizome layer was as well lower (being also lower at north- than at south-vent; PERMANOVA Pseudo-F = 13, p = 0.0001). Whilst habitat complexity in terms of canopy height and rhizome layer was lower at vents than off-vent (Fig. 6c), such differences were not consistent in terms of refuge provision (Fig. 6d). Indicators of habitat refuge provision corrected by the number of shoots showed a relatively lower corrected canopy height off-vent and north-vent than south-vent (PERMANOVA Pseudo-F = 6.6, p = 0.003), with the later also showing the highest corrected rhizome layer followed by off-vent (slightly not significantly different than south-vent; pairwise comparison p = 0.057) and with north-vent having the lowest value (PERMANOVA Pseudo-F = 10, p = 0.0004).
Discussion
Our study revealed that a key seagrass herbivore adjusts its feeding according to CO2/OA-mediated changes in the palatability of primary producers, thus maintaining its stoichiometry and trophic niche packing. Consumption by sea urchins was reduced on a less palatable seagrass and its epiphytes, while increased on higher-palatable green algae. Such urchin trophic plasticity reduced herbivory pressure on a seagrass that was subjected to a 5 to 8-fold higher fish herbivory under acidified conditions. This supports the hypothesis that the trophic plasticity of key herbivores may behave as a compensatory mechanism that helps to counteract habitat change and to promote system persistence under OA, as previously suggested for kelp forests14.
The constancy in consumer stoichiometry from off-vent to vents agrees with the Ecological Stoichiometry theory, by which elemental ratios of consumers remain constant despite the response of primary producers to changes in the availability of environmental resources21,22. This consistency was also observed in the trophic structure of sea urchin populations, as reflected by the similar niche width, number of basal resources exploited, trophic length, and packing at vents and off-vent. Sea urchin trophic plasticity was reflected by a shifted niche position at vents, where sea urchins reduced their feeding on Posidonia leaves and epiphytes that naturally dominate their diet (e.g. our results, ref. 23). Seagrass with epiphytes from off-vent and vents were equally palatable to sea urchins, while epiphyte removal shifted urchin preference towards off-vent seagrass. The intense fish herbivory at vents mainly removes the apical older parts of Posidonia leaves heavily colonized by epiphytes24, likely driving the lower seagrass ingestion via either, the loss of higher-quality epiphytes or the lowered palatability of a structurally resistant (thicker) seagrass at vents. Previous studies support this urchin feeding choices on Posidonia as mediated by both, epiphyte presence25,26 and seagrass structural defences25,27. Also, an experimental study conducted on the seagrass Cymodocea nodosa showed that P. lividus was deterred by CO2-enriched plants that were offered without epiphytes, likely due to changes in unidentified structural traits of the seagrass28. To overcome the CO2-mediated changes in the quality of their food resources at vents, sea urchins increased the ingestion of more palatable Cladophora and Flabellia. Whilst Flabellia was a common green alga elsewhere, Cladophora was less abundant at vents and scarce at off-vent. Thus, the amplified trophic role of these green algae was due to the reduced palatability of the main food source (namely, the seagrass), rather than a bloomed availability as often reported for turf-algae14. Concurrently, the feeding on algae instead on seagrass epiphytes at vents drives a lower urchin trophic position, with less degree of omnivory since epiphytes include a mix of algal and animal material (hydrozoans, bryozoans, polychaetes, ascidians, among others; see refs. 29,30).
We observed doubled predatory fish densities under acidified conditions, likely due to bottom-up benefits of high pCO2 on preys, or some other variables that we did not specifically measure. Our finding agrees with other studies in CO2 vents reporting an increased biomass of common site-attached carnivorous blennies or triplefins, which was related to a lower abundance of their predators31 or to the CO2-resource effect32. Wrasses at vents exploited a lower diversity of resources and occupied a lower SIA-based trophic position (higher degree of omnivory due to an amplified role of basal resources) than off-vent, although the first observation was constrained by a low replication. Contrastingly to SIA-based results, we found no difference between vent and off-vent in the diet-based trophic positions of wrasses obtained using the diet-based method. Such discrepancies are likely related to intrinsic methodological differences, since SIA indicates assimilated food over weeks-months and gut content analyses a snapshot of recently ingested diet biased towards less digestible resources that remain longer in the stomach33. This seems particularly relevant for omnivores/predators that feed on food items entailing a high degree of variation in size and digestibility. We found that the diet of this predatory fish was dominated by amphipods and gastropods at vents (51% of the diet versus 21% at off-vent), which have been reported to double their number at these vent sites34. Garrard et al.34 also found a reduced number of bivalve and isopod species under acidified conditions, which may explain their lower contribution and the lower diversity of food resources in wrasse diet at vents.
The abundance of sea urchins did not significantly differ at vents and off-vent, which agrees with previous observations in tropical and temperate vents under near-future OA scenarios35. The CO2-resource effect on herbivore abundance or a lacking cascade-effect from the increased predator density at vents are likely behind such observation, as summarized in Fig. 7. Indeed, the studied predatory fish fed on several preys other than sea urchins, thereby diluting the predation pressure in multiple directions. We also highlighted that a higher seagrass density at the south-vent compensates the lower seagrass canopy and rhizome layer that we found, thus helping to maintain the refuge provision to sea urchins. Population-level responses to OA may result, as well, from the combined effect of several other factors that we did not specifically measure, such as the evolutionary adaptation of urchin populations at vents via natural selection of resistant phenotypes36. Differential species sensitivities to stressor effects of lowered pH on calcification, acid-based regulations, or sensorial and behavioural functioning (e.g. altered prey escape response or predator detection of preys and attack speed) may also contribute to alter the size of herbivore populations, both directly (causing individuals’ mortality) and indirectly (modifying the strength of the top-down control by predators; see reviews 37,38).
Our observations at vents showed that OA-driven changes in plant or meadow traits may result in a severe fish herbivory that reduces the height of the seagrass canopy. Studies conducted at these vent sites in different years suggest that the intense fish herbivory that we observed is consistent over the years and significantly different from several control sites39,40. Furthermore, similar increased salema herbivory on seagrass has also been reported in other shallow Mediterranean vent systems41. This suggests that shallow meadows could be subjected to a substantial fish herbivory in near-future oceans. In the studied Ischia vent, fish herbivory is not causing shoot mortality or habitat shift. Indeed, it seems not to counteract the benefits of high pCO2, as reflected by the relatively higher seagrass shoot density at vents compared to various control sites reported by other studies34,39,40. We evidenced that the trophic plasticity of sea urchin populations at vents (reduced feeding on the habitat-forming seagrass) may act as a stabilizing mechanism of a system altered by an intense fish herbivory, thus helping to reinforce the system resistance to OA. This highlights that conservation efforts aimed at safeguarding the natural complexity of species interactions may help to minimize OA impacts in near-future oceans.
Methods
Study sites
The study was conducted during October 2013 in Posidonia meadows at 2.5–3 m depth in CO2-vent and off-vent sites along the northeast coast of the Ischia Island (Italy, NW Mediterranean Sea). Vent sites were at the north and south sides of the islet Castello Aragonese, which are 150 m apart one from each other and separated by a land bridge connecting the Castello islet to Ischia (40 °43′55.5/51.2″N, 13 °57′50.5/47.6″E). The off-vent site was Lacco-Ameno (40 °45′26.0″ N, 13 °53′4.5″ E), located away from any vent influence (6 km from vents) and representative of other control sites reported elsewhere regarding resource availability, stable isotopes of resources and consumers, or urchin abundances40,42,43. The pH level represented current conditions at the off-vent site and projections for 2100 in pH reduction at the vent sites (−0.30 to −0.32 pH units under the RCP8.5 scenario44; Supplementary Table S1). The gradient of volcanic CO2 bubbling at vent sites extended from 0.5 m to 3 m depth. Vent emissions are well characterized and release almost exclusively CO2 (with no toxic hydrogen sulphide) at ambient temperature45,46. Seawater pH and pCO2 measures at the vent sites are consistent over years and differ significantly from control sites40,42,46. The pH range of short-term fluctuations is similar to ranges naturally found in other marine habitats such as coral reefs or upwelling areas47.
Sample collection
The abundance of seagrass epiphytes and algal resources was estimated within four zones of ca. 15 m2 per site. Within each zone, algal samples were collected in amounts proportional to their abundance by pooling together in a bag samples equivalent to four quadrats of 20 cm ×20 cm, as well as searching around the zone for any other alga not present in the quadrats in order to truly cover all species available to herbivores. The most abundant basal resources, which were further considered for analyses of quality and stable isotopes (4 replicates from the pooled material) or feeding assays (n = 5–10 replicates; see Fig. 4), were the leaves of the seagrass Posidonia (pooled second-outermost leaves from 6 shoots), their epiphytes (removed from the seagrass leaves with a glass slide), the four most abundant algae (Flabellia petiolata, Halopteris scoparia, Jania rubens, and Peyssonnelia spp.), and the relatively abundant Cladophora prolifera (here named Cladophora). Other resources that were scarce in some meadows but present in urchin guts (Asparagopsis armata, Dictyota spp., and Cladophora spp.) were only collected for SIA (with 4, 3 and 1–2 replicates, respectively).
In each site, 10 sea urchins and 3 wrasses were caught (no wrasse was caught at the north-vent), euthanized, measured and dissected for extraction of guts and muscle samples. They were adult individuals of similar size (mean ± SD; sea urchins: 5.1 ± 0.5 cm in diameter without spines; wrasses: 13.1 ± 0.7 cm in total length). A sample of white muscle was collected under the dorsal fin from wrasses, while the Aristotle’s lantern muscle was collected from sea urchins. Samples were freeze-dried and reduced to fine powder for laboratory analyses.
Consumer feeding
Carbon and nitrogen stable isotopes (plus C and N content) of producers and consumers were analysed with an isotope ratio mass spectrometer (ThermoFinnigan DeltaPlus) coupled to an elemental analyser (ThermoFinnigan FlashEA1112). They were expressed in delta notation, as parts per thousand deviation from the corresponding standard reference materials (Vienna Pee Dee Belemnite and atmospheric N2). Analytical precision based on the deviation from internal standards was ±0.15‰. Samples of epiphytes and calcified algae were acidified (HCl 2 N) prior to δ13C analysis to remove carbonates. Phosphorus (P) content was determined using inductively coupled plasma optical emission spectrophotometry (Perkin Elmer, Optima 8000), after acid (HNO3/H2O2) digestion. To obtain enough material for P analysis, samples of sea urchin muscle were pooled (vents n = 3; off-vent n = 4). CNP ratios were calculated on a molar basis.
Isotopic data of sea urchin populations (n = 10 for each site) were used to estimate the Layman metrics of trophic structure48 and the Standard Ellipse Areas using the SIBER package v2.0.2 in R49. Specifically, the δ15N range (NR) and δ13C range (CR) are the Euclidean distance between the highest and the lowest δ15N and δ13C values. NR represents the trophic length or the degree of omnivory (high NR = more individuals feeding at different trophic levels) and CR the diversity of basal resources exploited (high CR = more carbon sources). The mean distance to centroid (CD) is the average Euclidean distance of each individual to the mean δ15N and δ13C values of all individuals in the population and represents the trophic diversity. The mean nearest neighbour distance (NND) measures the density of individuals’ packing or trophic redundancy. The standard deviation of the nearest neighbour distance (SDNND; less influenced than NND by sample size) measures the evenness of the population packing (low NND and SDNND = individuals in a population with similar trophic habits). Metrics of urchin populations were bootstrapped (n = 10000; indicated with a subscript ‘b’) to allow comparison among populations50. We estimated the isotopic niche width of urchin populations from the Bayesian Standard Ellipse Area (SEAb; with SIBER default priors and Monte Carlo options) and the corrected Standard Ellipse Area (SEAc, corrected for small sample size). Differences between sites in bootstrapped metrics and Bayesian ellipse areas were tested regarding their dissimilarity (p > 0.95) using the probability test proposed in the SIBER package. Due to the low replication of predator populations (n < 5), we were only able to estimate single values of Layman metrics using SIBER (but not bootstrapped metrics or standard ellipses), whose differences cannot be tested for statistical significance. Furthermore, we examined differences between sites in the position of the niche centroid using a bivariate permutational analysis of variance (PERMANOVA) on δ13C and δ15N values grouped by site and separately tested for sea urchins (3 sites: north-, south- and off-vent) and wrasses (2 sites: south- and off-vent).
Diet composition was estimated as the proportion of resources/preys in consumer guts (% of horizontal surface covered) that were identified and quantified under a stereomicroscope. Labrid fishes feed on sea urchins by entirely swallowing small recruits (<1 cm) or by eating soft meat after opening the test of larger juveniles that they are able to bite (1–3 cm)17. This limited the recognition of sea urchins in wrasse gut to rests of long-lasting calcareous structures (Aristotele lantern, skeleton or spines) of small recruits that were entirely swallowed. Very deteriorate pieces of such tiny rests were likely included in the category ‘Other’. Taking that into account, a MixSIAR model in R51 was used to estimate, and further confirm, the role of sea urchins in wrasse diet (Supplementary Table S2). Sea urchin diet was obtained from Nogueira et al.24. Similarity percentage analyses (SIMPER) were used to identify food items in each consumer diet with higher and consistent contribution to the between-site dissimilarity. A consistent contribution (namely, higher than variation) was arbitrarily defined as that with a mean dissimilarity to standard deviation ratio> 1.5.
We obtained consumer trophic positions (TPc) from diet data (excluding ‘other material’) by using an equation modified from Vander Zanden & Rasmussen52 as follows: TPc = ∑(Ci.TPi) + 1, where Ci = contribution of the prey i to the diet, and TPi = trophic position of the food item i obtained as detailed in Supplementary Table S3.
We also estimated consumer trophic positions from SIA data using the equation proposed by Post53: TPc = [(δ15Nc − δ15Nb)/∆δ15N] + λ, where subscript c and b respectively refers to the consumer and to the baseline reference species; ∆δ15N is the expected enrichment in δ15N between successive trophic levels; and λ is the trophic position of the species chosen as baseline. The baseline for sea urchins, with a trophic level λ = 1, was the site-specific mean δ15N value of the main basal resources (those with abundance > 2% and a total contribution of ca. 90% to the diet, excluding epiphytes). The baseline for wrasses, with a trophic level λ = 2, was obtained from primary consumers showing the lowest δ15N values (site-specific values of other benthic invertebrates obtained from Ricevuto et al.43 in the same sites and season). The isotopic fractionation considered was ∆δ15N = 2.52‰ for the herbivore and 3.23‰ for the predator54.
Resource quality and palatability to herbivore
Indicators of nutritional (CNP contents and ratios obtained as described above, carbohydrates) and chemical quality (total phenolics) were measured on the most abundant basal resources available to herbivores. Non-structural carbohydrates were measured using the phenol-sulfuric acid colorimetric method55 with glucose as standard, after extraction of soluble sugars (mostly sucrose) in hot ethanol and enzymatic conversion of starch to glucose equivalents. Total phenolics were extracted with 80% methanol for 24 h and determined with spectrophotometer after a Folin-Ciocalteu assay using caffeic acid as standard (modified from Bolser et al.56). Only 3 replicates were available for P, phenolics and sucrose/starch analyses of Cladophora at the off-vent. Five replicates of Posidonia and Peyssonnelia were analysed per site for phenolic content because a high variability among replicates was detected.
The carbonate content of calcareous resources (epiphytes, Jania and Peyssonnelia) was quantified as structural defence by the loss of dry weight after decalcification of samples with HCl (2 N). Structural traits of seagrass leaves were measured in the second-outermost (fully-developed) leaf of 14 of those shoots used to estimate fish herbivory. Leaf width and thickness were measured at 3.5 cm above the sheath junction with an electronic digital thickness gauge (Miyare, precision ± 0.01 mm), and then multiplied to calculate leaf cross-sectional area. Three of the 14 replicates from the north-vent were lost during sample processing.
We conducted feeding preference assays to investigate whether resources are more or less palatable to sea urchins in response to OA. Assays were conducted in 24 L aquaria with aerated seawater offering comparable amounts of off-vent and vent material of each resource to an adult sea urchin (5.4 ± 0.5 cm diameter, n = 42). A relatively old tissue of Posidonia leaves was used (ca. 8 cm2 of the second-outermost leaf, avoiding necrotic apices), as this is preferentially eaten over young tissue by this sea urchin species25. Fresh weight offered varied depending on the resource (ca. 0.3 g Posidonia, Flabellia, and Peyssonnelia; ca. 0.5 g Halopteris; and ca. 1 g Jania). For each resource, 5 to 10 assay replicates were set up, as well as 3 to 4 control replicates with resource material and no sea urchin to correct consumption for autogenic changes during assays. Prior to the start of the assays, sea urchins were acclimatized and starved for 24 hours. Assays were ended when 50% of any resource material was consumed. Consumption of each resource (% of the initial wet mass offered that was consumed) was calculated as [(Hi × Cf/Ci)−Hf] × 100/Hi, where Hi and Hf are initial and final wet masses of the resource and Ci and Cf the mean initial and final wet masses of the corresponding autogenic controls. All assays were performed in accordance with appropriate guidelines and regulations.
To examine the significance of the preference, we estimated the effect size using Hedges’ d (the difference in consumption means between off-vent and vent weighted by the sample size and the pooled standard deviation) and its confidence intervals (CI)57, since our data followed a normal distribution. When CI did not include zero, the effect size was significant at the 5% level.
Habitat structure
The structural complexity of the seagrass habitat was estimated as the heights of the leaf canopy and of the unburied rhizome layer in 25 random areas of 20 cm × 20 cm per site. The canopy height was measured from the base to the top of the leaves ignoring the 20% of the tallest ones (modified from Short & Coles58). The unburied level of the rhizome layer was measured as the distance between the leaf base and the sediment surface by inserting a ruler into the three-dimensional net formed by the overlapping vertical and horizontal rhizomes59. Both variables, together with seagrass shoot density, are related to refuge provision of Posidonia meadows to sea urchins60. Corrected indicators of refuge provision were obtained by multiplying each replicated valued of canopy and rhizome heights by the number of shoots in the sampling area using shoot density data at the same sites and depth obtained from the literature (mean value per site).
Fish (salema) herbivory is known to mediate indirect interactions operating at local spatial scales by reducing the seagrass canopy height and the refuge provision, with a subsequent increase in the predation of sea urchins61. To take this into account, we quantified the percentage of leaves with fish bite marks in one shoot within each of the 25 above-mentioned areas. Bite marks of salemas were easily differentiated due to their characteristic half-moon shape62.
Consumer abundance
We quantified consumer abundances in each site using visual censuses along five (5 m length × 2 m width) transects for sea urchin and six (10 m length × 4 m width) transects for fish. Consumer abundances were expressed as number of individuals per squared meter. Whilst transects in each meadow were surveyed during the morning in a single day, results were consistent to divers’ observations at different times on several days. P. lividus was the only urchin species found. Adult sea urchins found in transects were part of those collected for SIA, diet analysis and feeding assays.
Other data analyses
Differences between sites were analysed separately for each variable using PERMANOVA, since most of our data did not conform to parametric test assumptions. We used one-way PERMANOVAs to examine differences among sites (fixed factor, 3 levels) in seagrass canopy height, rhizome layer, fish herbivory, and leaf thickness, width, and cross-sectional area, as well as in consumer isotopic signatures, elemental contents and ratios, trophic position and diet composition analysed separately for urchins and wrasses (3 and 2 levels, respectively, as no wrasse was caught at the north-vent). Two-way PERMANOVAs were used to examine differences between sites (3 levels) and resources in isotopic signatures (resources: 10 levels), quality traits and CNP contents or ratios (resources: 3 levels for calcification and 7 levels for the rest), as well as between sites and consumers (2 levels) in their abundances. We calculated Euclidean distances from untransformed data and used 9999 unrestricted permutations of raw data to perform the tests, except in consumer abundances and fish herbivory (both (x + 0.001)-transformed data) or consumer diet (untransformed data) for which Bray-Curtis distances were used. When significant differences were found, pairwise comparisons were performed. When the number of possible permutations was low (<100) in any PERMANOVA test or pairwise comparison, the Monte Carlo p-value was used instead of the permutation p-value63.
Data availability
The data that support the findings of this study are included in the figures of the article and its supplementary information. The raw data used to generate the figures and the R codes used to analyse them are available from the corresponding author upon reasonable request.
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Acknowledgements
The research was funded by an ASSEMBLE access project within the EU FP7/2007–2013 program (grant agreement n° 227799) hosted by MCG at the SZN. The Portuguese FCT– Fundação para a Ciência e a Tecnologia funded BMC in the ambit of the contract program DL57/2016/CP1361/CT0004 and CCMAR through the project UID/Multi/04326/2019. We thank the SZN staff, and in particular to Captain V. Rando for valuable help during fieldworks and M. Lorenti for technical support in the laboratory. We also thank G. Salvati for support in the fieldworks and the CRC-team for help in processing producers’ samples.
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B.M.C., S.V., M.C.G., G.C. and R.S. conceived and designed the study. G.C. conducted fieldworks, and with M.C.G. and B.M.C. collected producer and sea urchin samples that were processed by B.M.C. and G.C. Laboratory analyses of producers’ quality traits were done by B.M.C. A.M.G. processed fish samples for SIA analyses and performed the fish gut content analysis. B.M.C. and C.A. analysed the data. BMC produced figures and wrote the manuscript, and all co-authors contributed to revisions.
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Martínez-Crego, B., Vizzini, S., Califano, G. et al. Resistance of seagrass habitats to ocean acidification via altered interactions in a tri-trophic chain. Sci Rep 10, 5103 (2020). https://doi.org/10.1038/s41598-020-61753-1
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DOI: https://doi.org/10.1038/s41598-020-61753-1
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