Abstract
Efforts to produce aromatic monomers through catalytic lignin depolymerization have historically focused on aryl–ether bond cleavage. A large fraction of aromatic monomers in lignin, however, are linked by various carbon–carbon (C–C) bonds that are more challenging to cleave and limit the yields of aromatic monomers from lignin depolymerization. Here, we report a catalytic autoxidation method to cleave C–C bonds in lignin-derived dimers and oligomers from pine and poplar. The method uses manganese and zirconium salts as catalysts in acetic acid and produces aromatic carboxylic acids as primary products. The mixtures of the oxygenated monomers are efficiently converted to cis,cis-muconic acid in an engineered strain of Pseudomonas putida KT2440 that conducts aromatic O-demethylation reactions at the 4-position. This work demonstrates that autoxidation of lignin with Mn and Zr offers a catalytic strategy to increase the yield of valuable aromatic monomers from lignin.
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Introduction
Lignin is the most abundant natural source of aromatic units, comprising 15–30 wt% of lignocellulosic biomass1,2, and many contemporary efforts are pursuing lignin valorization via depolymerization to aromatic monomers1,3,4,5,6,7. Multiple depolymerization strategies have been developed that cleave aryl–ether C–O bonds very effectively3,4,5,6,8; however, the various C–C linkages in lignin, formed during its biosynthesis9 or from condensation reactions during lignocellulose processing6,10, are much more difficult to cleave11.
To date, very few catalytic methods have been reported to cleave C–C inter-unit linkages in lignin. A notable example uses a Ru/NbOPO4 catalyst that cleaves both C–O and C–C bonds and yields deoxygenated lignin oils with up to 32 wt% monomers. This system generates monocyclic products in significantly higher yields (up to 153 mol%) than those accessible from nitrobenzene oxidation, a method that primarily cleaves C–O linkages12,13. Wang et al. reported that up to 13 wt% of phenol can be obtained through tandem C–O and C(aryl)–C bond cleavage using a multifunctional catalyst comprising CuCl2/AlCl3 and Ru/CeO214. Similarly, Han and colleagues reported an 8 wt% yield of phenol with a zeolite catalyst15. Stahl and colleagues reported oxidative catalytic fractionation of lignin affording C–C cleavage products16, and Waldvogel and colleagues showed vanillin could be obtained by oxidation of kraft lignin with peroxodicarbonate17. Other methods may generate products of C–C cleavage, but mechanistic information is lacking, the monomers are formed yields below values that can be obtained through C–O cleavage alone, or the processes require multiple steps and proceed in lower yields18,19,20,21.
Lignin oil from reductive catalytic fractionation (RCF) is an ideal substrate to explore C–C bond cleavage in lignin due to the near-theoretical C–O bond cleavage that occurs during this process, resulting in an oil wherein the dimers and oligomers are only linked by C–C bonds (Fig. 1)10,22,23,24. Recently, Samec and coworkers demonstrated that a TEMPO+-derived oxidant [TEMPO= (2,2,6,6-tetramethylpiperidin-yl)−1-oxyl] is able to produce 1.9 mmol of 2,6-dimethoxybenzoquinone/g of oligomers derived from RCF of birch wood25. The reaction uses 400 wt% of TEMPO+ oxidant with respect to the lignin-derived substrate, although the oxidant could be regenerated electrochemically and re-used to afford similar product yields in subsequent cycles.
Despite these promising reports, there remains a need for catalytic systems that can cleave C–C bonds in lignin to utilize lignin more effectively and offer product flexibility for lignin valorization to useful products. One option is autoxidation, a radical-chain process that is widely used in the commodity chemical industry (e.g., in the production of phenol from cumene26, cyclohexanol/cyclohexanone27, terephthalic acid and other aromatic carboxylic acids28,29,30,31,32, and alkyd paints33,34). Co, Mn, and other metal ions are often used as catalysts in these processes, leveraging their ability to break down hydroperoxides into reactive alkoxyl radicals via the Haber-Weiss reaction28,31,35. The resulting alkoxyl radicals can undergo C–C bond cleavage via β-scission. Seminal reports from Partenheimer demonstrated production of aromatic carboxylic acids from 3,4-dimethoxytoluene, as a simple lignin model, and from various lignin sources using Co/Mn/Br and Co/Mn/Zr/Br catalyst systems, respectively36,37. In two recent studies, we demonstrated C–C bond cleavage in mixed plastics and an acetyl-protected poplar RCF oligomer substrate using a Co/Mn autoxidation catalyst system paired with a N-hydroxylphthalimide or bromide co-catalyst, respectively38,39,40.
In an attempt to develop catalyst systems that use more abundant and sustainable metals, here we present an autoxidation catalyst for C–C bond cleavage that avoids the need for cobalt41. Further, we sought to eliminate bromide as it is corrosive and necessitates the use of expensive titanium-clad reactors due to its corrosivity29. Studies comparing oxidations of an acetyl-protected lignin model demonstrated Mn is not active enough for appreciable C–C bond cleavage. However, we observed C–C bond cleavage with Mn when studying the methyl-protected analog, 4-propylveratrole, in the presence of a Zr co-catalyst. Thus, in this work we use a homogeneous Mn and Zr aut-oxidation catalyst system to cleave the C–C bonds in oligomers of methylated pine (primarily G-type lignin) and poplar (G- and S-type lignin) RCF oils, along with the model compounds 1–6 shown in Fig. 2. We demonstrate that this Mn/Zr catalyst system produces a mixture of bio-available monomers that can be converted to cis,cis-muconic acid using biological funneling38,42,43,44,45, a strategy that utilizes genetically engineered microbes to convert mixtures of compounds into a single target product, with a strain engineered to demethylate the methyl-stabilized phenol42,46. An overview of the workup procedure from biomass to cis,cis-muconic acid is shown in Supplementary Fig. 1.
Results
Methyl protection of phenolic groups enables lignin autoxidation with Mn and Zr only
We first explored oxidations of mono-aromatic model compounds with propyl side chains to test whether C–C bond cleavage can be achieved using Mn-catalyzed autoxidation, relative to the Co/Mn/Br system previously studied, which cleaves the C–C bonds of acetyl-protected lignin aromatics40. As we will present below in more detail, we observed C–C bond cleavage products with methyl-protected substrates in the presence of a Zr co-catalyst. The “standard conditions” shown in Fig. 3 are those determined to be optimal for the RCF oligomer substrate (vide infra), and the yields for model compound experiments are reported in mol% with respect to the number of aromatic rings needed to produce the product. The oxidation mixtures were derivatized by methylation prior to analysis using gas chromatography with a flame ionization detector (GC-FID) for accurate quantification of the carboxylic acids (Supplementary Figs. 2 and 3; Supplementary Tables 1 and 2). The model compounds and analytical standards were synthesized as detailed in the ESI (Supplementary Figs. 4–27). The complete distribution of products of the reactions in Figs. 3 and 4, as discussed in further detail below, can be found in Supplementary Figs. 28 and 29 of the ESI.
As a negative control reaction, we initially attempted to oxidize 4-propylguaicol, 1-OH, a coniferyl (G)-type monomer, as shown in Fig. 3. Treatment of 1-OH at 6 bar O2 and 150 °C with 8 mol% Mn(OAc)2•4H2O and 6 mol% Zr(acac)4 (acac = acetylacetonate) as catalysts in a steel reaction vessel resulted in 94 ± 1% conversion (hereafter denoted using 94(1)% notation) of 1-OH to several products, but only 2.1(6) mol% of vanillin was obtained as a result of C–C bond cleavage. The product of C–C bond coupling, divanillin, was produced in 11(3) mol% yield.
Due to the low recovery of aromatic products with 1-OH, attributed to the reactivity of phenolic compounds under oxidizing conditions, we next sought oxidations of lignin models with phenol-stabilizing groups. The attempted oxidation of the acetyl-protected analog of 1-OH, namely 4-propylguaiacyl acetate, 1-Ac, with a Mn catalyst resulted in 4.6(1)% conversion of the substrate and no appreciable yield of C–C bond cleavage products. When Zr was added, 18(1)% of the substrate was converted, but products were produced at only 1.9(7)%. This demonstrates that both Mn and Mn/Zr are less active catalysts than our previously reported Co/Mn/Br system40, which converts 82(10)% of the 4-propylguaiacyl acetate in 2 h at 120 °C and produces 47(8)% of products through C–C bond cleavage40.
We hypothesized that changing the phenol-stabilization group to one that is more electron-donating would enable the Mn catalyst to oxidize the lignin model substrate. Consequently, we targeted the methyl-protected analog of 4-propylguaiacol, 1-OH, 4-propylveratrole, 1, along with 1-(3′,4′,5′-trimethoxyphenyl)propane, 2, the methyl-protected analog of the syringyl (S)-type 4-propylsyringol, as shown in Fig. 3. Treatment of 1 under the standard oxidation conditions yielded 30(8) mol% of products comprising 13(7) mol% of veratric acid, 5.5(3) mol% of veratraldehyde, 5.1(9) mol% of 2-acetoxypropioveratrone, and 6.2(5) mol% of 2-methoxymaleic acid, the lattermost evidently arising from ring opening of 1. Similarly, 2 exhibited a total product yield of 48(2) mol%, including a 14.5(6) mol% yield of 3,4,5-trimethoxybenzaldehyde and 23(1) mol% yield of 3,4,5-trimethoxy-benzoic acid. Like the reaction of 1, the 2-acetoxy aryl ketone was also produced, in 10.5(5) mol% yield. In the absence of the Zr co-catalyst known to improve autoxidation selectivity and prevent MnO2 precipitation oxidation28,47,48, 1 yielded only trace quantities of products.
The aromatic acid and aldehyde products exhibit limited stability under catalytic conditions
Subjecting the S- and G-type aromatic aldehyde and carboxylic acid products to the standard conditions resulted in reduced aromatic product recovery, as shown in Fig. 4. For example, veratric acid was recovered in 62(7) mol%, whereas veratraldehyde was recovered in 38 mol% (5(1) mol% veratraldehyde and 33(4) mol% as veratric acid). Similarly, 3,4,5-trimethoxybenzoic acid was recovered in 51(12) mol%, and 3,4,5-trimethoxybenzaldehyde was recovered in 43 mol% (20 mol% aldehyde and 23 mol% as 3,4,5-trimethoxybenzoic acid). Each substrate produced 2-methoxymaleic acid, which is indicative of oxidative ring-opening.
Dimer model compound oxidation reactions with the Mn/Zr catalyst system demonstrate monomer production through C–C bond cleavage
In a similar manner, the methylated dimers 3–6 (Fig. 2) were oxidized using the standard reaction conditions, as shown in Fig. 5. Notably, we discovered that this catalyst system liberates monomers from 3 of the 4 types of C–C bonds studied (β−1, β−5, β-β).
The model β−1 dimer, 1,2-bis(3,4-dimethoxyphenyl)ethane, 3, when oxidized, gave a total product yield of 35(4) mol% aromatics consisting of 29(3) mol% of veratric acid, 2(2) mol% veratraldehyde, and 4.4(4) mol% of 2-methoxymaleic acid. Oxidation of the β-β model compound eudesmin, 4, afforded a total product yield of 45(2) mol% comprising 37(2) mol% veratric acid, 4.4(1) mol% veratraldehyde, and 2.9(2) mol% methoxymaleic acid. The β−5 dimer, 5, gave a total product yield of 30(1) mol% of veratric acid, 12.5(8) mol%, veratraldehyde 15(1) mol%, 2-methoxymalelic acid 2.0(4) mol%, along with trace quantities of 3,4-dimethoxyisophthalic acid.
The 5-5 dimer model, 1,1′-dipropyl-3,3′,4,4′-tetramethoxybiphenyl, 6, did not yield monomer products in significant quantities and afforded 30(1) mol% of products comprising 13.7(7) mol% of the dimethyl divanillate, 7.6(4) mol% of dimethyl divanillin, and 6.9(4) mol% of the mixed acid/aldehyde analog. Additionally, 2.1(1) mol% of the monocyclic 3,4-dimethoxyisophthalic acid was identified, evidently due to ring cleavage of one of the aromatic units.
RCF oil methylation followed by distillation separates monomers and produces an oligomer substrate for autoxidation
RCF was used to produce lignin oils from pine and poplar using a 5 wt% Ru/C catalyst in methanol at 225 °C for 6 h with 30 bar H2 (Supplementary Figs. 30–34; Supplementary Tables 3–7)49. The total yields of monomers were quantified by GC-FID to be 1.5 mmol/g of phenolic pine RCF oil, and 1.8 mmol/g of phenolic poplar oil. Phenol stabilization by methylation of the RCF oils was accomplished by stirring the oils in acetonitrile with excess potassium carbonate and methyl iodide at room temperature for 60 h. For pine, the monomers were recovered quantitatively as their methylated analogs. For poplar, near-quantitative recovery was observed for all monomers except the S-type monomers 4-propenyl-2,6-dimethoxyphenol (44% recovery) and 3-(4-hydroxy-3,5-dimethoxyphenyl)−1-propanol (8% recovery). Residual phenols in the methylated oils were not detected by 31P NMR spectroscopy (Supplementary Fig. 35 and Supplementary Table 8).
Distillations of the methylated oils were performed at 220 °C and 10 mbar for 30 min. The GC-FID quantification data of monomers in the oligomer and distillate fractions from the pine distillation showed a monomer recovery of 98% in the distillate collection flask with <1% remaining in the bottoms. The distillation of methylated poplar oil also gave high recoveries: 95% of the monomers were recovered with only 3% detected in the bottoms fraction. The gel permeation chromatography (GPC) data are consistent with the quantification data and the successful removal of monomers to produce oligomer-enriched substrates for both pine and poplar, as shown in Fig. 6. A combination of GPC, GC-FID, and gas chromatography-mass spectroscopy (GC-MS) data on the pine distillate and analytical standards shown in Fig. 6b and Supplementary Fig. 33 indicated that dimers were present in the distillate.
Autoxidative C–C cleavage affords monomers at 28 mol% of the RCF yields
We next sought to optimize the autoxidation catalysis conditions using the methylated pine oligomer substrate, including reaction temperature, oxygen loading, catalyst loading, substrate loading, reaction time, and Zr loading, as shown in Fig. 7. As done for the model compounds, the oxidation mixtures containing carboxylic products were derivatized by methylation prior to GC-FID analysis (ESI Methods). The yields are reported in mmol analyte/g of substrate in the bar charts with wt% (total g pre-derivatized analytes/g substrate x 100) yields reported above each bar. Numerical values for the optimization conditions are presented in Supplementary Tables 9–14.
A screen of six reaction temperatures (Fig. 7a) ranging from 90–190 °C showed a distribution of yields that increased sharply from 0.23 mmol/g of oligomer substrate at 130 °C to the maximum yield of 1.2 mmol/g at 150 °C. At 170 °C, the yield decreased to 1.1 mmol/g and to 0.84 mmol/g at 190 °C. The oxygen partial pressure also affected monomer yields (Fig. 7b). At PO2 = 0 bar, no monomers were detected, as expected. There was an increase in total monomer yield from PO2 = 1 bar, which gave a yield of 0.86 mmol/g of oligomer substrate, to 1.2 mmol/g at 6 bar. An oxygen loading of PO2 = 8 bar did not improve the overall yield. An increase in total monomer yield was also observed with increased catalyst loadings (Fig. 7c) and substrate concentrations (Fig. 7d). When changing the catalyst loading while maintaining a substrate quantity of 50 mg, the total product yields increased from 0.032 mmol/g of pine oligomer substrate with 0 wt% catalyst to 0.16 mmol/g with 4 wt% Mn(OAc)2•4H2O and 6 wt% Zr(acac)4. Using 8 wt% Mn(OAc)2•4H2O and 12 wt% Zr(acac)4, a substantial increase in the product was observed (1.00 mmol/g of oligomer substrate). Increasing catalyst loadings further did not produce significantly higher product yields. Similarly, while maintaining a constant catalyst loading of 8 wt% Mn(OAc)2•4H2O and 12 wt% Zr(acac)4, an increase in the total monomer yield was observed from 0.63 mmol/g with a 1.3 mg/mL concentration of substrate to 0.92 mmol/g with a 4.3 mg/mL concentration of substrate. A time course study demonstrated an increase in the total monomer yield from 0.71 mmol/g at 0.17 h to 0.92 mmol/g at 0.5 h (Fig. 7e). For reaction times between 0.5 h and 1.5 h, similar yields ranging 0.91–1.1 mmol/g were observed. Longer reaction times did not increase the total monomer yield. Lastly, we studied the effect of Zr loading. As shown with 1 in Fig. 3, the presence of Zr(acac)4 improved the yield of products (Fig. 7f). Similarly, when Zr(acac)4 was omitted from the oligomer oxidation reaction, only 0.061 mmol/g of identifiable products were quantified (primarily veratric acid and aldehyde). With a loading of 6 wt% Zr(acac)4, the overall yield increased to 0.77 mmol/g, and 12 wt% yielded 0.90 mmol/g. No substantial increase was observed with 24 wt% Zr(acac)4.
Using the optimized conditions identified from the experiments presented in Fig. 7, a total monomer yield of 1.01(4) mmol/g of oligomer substrate was obtained for the oxidation of the methylated pine oligomer substrate, as shown in Fig. 8a–c. For poplar, we applied the same conditions and obtained 0.96(2) mmol/g, as shown in Fig. 8d–f. The 1.01(4) and 0.96(2) mmol/g total monomer yields obtained from pine and poplar, respectively, each represent 28 mol% of the total monomer yield of their respective RCF process. A representative calculation for pine is presented in Eqs. (1) and (2) below. Numerical values of the autoxidation yields are presented in Supplementary Tables 15−16.
Engineered bacteria enable the conversion of pine oxidation mixtures to cis,cis-muconate
Aromatic-catabolic microbes can catabolize heterogenous mixtures of aromatic compounds toward a single target product through a process termed biological funneling38,42,43,44,45. The oxidative catalytic approach described above generates a slate of low molecular weight, water-soluble products, which are ideal for use as microbial growth substrates50. Here, strains of Pseudomonas putida KT2440 (hereafter, P. putida) were engineered to produce cis,cis-muconic acid from the aromatic monomers in the oxidation streams of methylated pine oligomers51. As shown in Fig. 8a, veratric acid and veratraldehyde comprised a large fraction (0.76 mmol/g substrate) of these streams, but these p-methoxylated compounds are not natively assimilated by P. putida (Supplementary Fig. 36). Fortunately, a cytochrome P450 monooxygenase from Rhodopseudomonas palustris HaA2 catalyzes p-demethylation of veratrate52, so this operon (CYP199A4-HaPux-HaPuR) was genomically integrated into P. putida under control of the strong Ptac promoter with optimized ribosome binding sites for each gene, generating strain ACB236 (Supplementary Table 18). Additionally, preliminary experiments indicated that the native vanillate demethylase, VanAB, could O-demethylate veratrate to isovanillate (Fig. 9A and Supplementary Fig. 37), so the isovanillate demethylase operon ivaAB53 was integrated to prevent undesirable accumulation of isovanillate, resulting in P. putida strain ACB262. The ESI Methods and Supplementary Tables 17–20 provide further details on strain construction and genotypes.
To ensure that P. putida could utilize the major constituents of the oxidized RCF oligomer stream, strain ACB262 was cultivated in an M9 minimal medium with 2 mM veratrate or 2 mM veratraldehyde as the sole source of carbon and energy. In both cases, the model compound was fully consumed within 50 h, and no accumulation of isovanillate was observed (Supplementary Fig. 38a, b). Next, strain ACB262 was evaluated for its ability to grow with oxidized oligomer streams as the sole source of carbon and energy. To prepare substrates for growth experiments, methylated oligomers of pine RCF oil were oxidized in triplicate at the 65 mg scale, treated with an aqueous base to precipitate catalyst, neutralized, and filter sterilized (ESI Methods)38. The resulting solutions were added at 10% v/v to M9 minimal medium, with initial media compositions provided in Supplementary Table 21. When P. putida ACB262 was grown in M9 with 10% v/v of oxidized RCF oligomers, it simultaneously utilized both veratrate and veratraldehyde from these streams without the need for a supplemental carbon source and without accumulation of intermediates (Supplementary Fig. 38c–e).
Additional metabolic engineering was required to produce muconate from veratrate and veratraldehyde in P. putida. A previously described strain, CJ78151, was modified to include the optimized CYP199A4-HaPux-HaPuR and ivaAB operons for p-demethylation as described above, as well as the mutant AroYE474V for decarboxylation of protocatechuate to catechol (Fig. 9A)54. The genotype of the resulting P. putida strain, ACB263, is shown in Supplementary Table 20. To verify functionality of the engineered pathway, strain ACB263 was grown with the model compounds veratrate and veratraldehyde, and glucose was added to 5 mM every 24 h to support growth. Veratrate and veratraldehyde were both consumed within 48 h and fully converted to muconate at 90.4(7)% and 96(2)% yields, respectively, with minimal accumulation of intermediates (Fig. 9B, C). Strain ACB263 was also cultivated with the same oxidized RCF oligomer streams as ACB262, and it fully converted the major monomer constituents (veratrate, veratraldehyde) to muconate within 24 h, achieving 100(2)% yield from these two substrates (Fig. 9D and Supplementary Fig. 39). Importantly, uninoculated control media incubated under the same conditions did not exhibit considerable changes in composition (Supplementary Fig. 40), indicating that all measured changes in analytes were associated with the action of the bacteria. Of note, p-methoxybenzoate was detected in small amounts in the oxidation streams of methylated pine RCF (Fig. 8a), and it is a viable substrate for the CYP199A4 system52. However, p-methoxybenzoate was not analyzed because its contribution to the total muconate yield was assumed to be negligible in these dilute biological preparations.
Discussion
These results demonstrate the catalytic production of bio-available monomers through C–C bond cleavage, as shown unambiguously using oligomers prepared from pine and poplar RCF oils, and their biological conversion to a single product, cis,cis-muconic acid. This Mn/Zr autoxidation catalyst system allows cleavage of the aryl propane units in 3 of the 4 types of carbon–carbon linkages present in lignin, shown in Fig. 1 (β-1, β−5, β-β).
While oxidations of lignin models and lignin have been studied with Co/Mn/Br and Co/Br catalysts36,37,55, the target of those studies was not C–C bond cleavage. Rather, the goal was to increase monomer yields from the oxidation of β-O-4-containing substrates by acetylation of the generated phenolic products in situ prior to their degradation under the oxidation conditions. Conversely, our study presents a method to overcome the inherent limit of monomer yields from C–O cleavage alone by catalytically cleaving C–C bonds in lignin oligomers. This is accomplished by effecting β-scission of the high-energy radical intermediates generated via autoxidation. We further describe methods that enable the identification and quantification of a mixture of oxygenated products along with a gene editing protocol that enables its biological conversion using engineered strains of Pseudomonas putida.
While promising, the overall process performed in batch reactors is limited by the need for stabilization chemistry and limited product stability, as observed in our model compound oxidations in Figs. 3–5. Although autoxidation catalysis for lignin warrants phenol stabilization, there are several options that could facilitate progressing this chemistry further. One approach to improve the viability of lignin methylation could be to use a flow system with dimethyl carbonate as a methylating reagent56,57,58,59. Further, the regeneration of dimethyl carbonate from carbon dioxide is an active area of study and would enable a circular methylation process60,61,62. Flow chemistry could also be used to overcome issues with aromatic decomposition. The products could be continuously removed from the reaction in a flow reactor, which could enable lignin oxidation without the need to separate out the initial monomers generated from RCF. Alternatively, if the RCF monomers with propyl chains are sufficiently valuable, other separation methods could be employed including advanced chromatographic techniques and membrane separations63,64,65,66 that allow monomer separation and preparation of RCF dimers and oligomers.
Mechanistic details of Co/Mn/Br/Zr-mediated autoxidations in acetic acid have been studied for decades, but to date, there is not a universally accepted model. This is likely because kinetic parameters vary substantially with the reaction conditions and substrate67,68,69,70. Dimeric and trimeric acetate complexes of Co(III) and Mn(III) have been proposed as intermediates71,72,73,74, and differences in the activity between various Co(III) species have been observed spectroscopically along with the decay of active Co(III) to more stable forms28,74,75,76,77. Less reactive forms have been proposed to exist as bridged hydroxo Co(III) dimers75,76,77, as well as hydroxo-substituted oxo-centered trimers74. But their interconversion with less hydroxylated structures at elevated temperatures has been described by varying water concentrations71. Regarding Zr, its role in the oxidation of RCF oligomers is not obvious based on product yields and distributions. In the industrial Co/Mn/Br mediated autoxidation of alkylbenzenes, Zr is used as a co-catalyst, especially for the oxidation of poly(alkyl)benzenes28. Several hypotheses based on kinetics data involving Co have been proposed to explain the increase in activity when Zr is added28,30,47,68,78,79,80,81. Chester et al. proposed from kinetics models that Zr forms heterobimetallic complexes with Co(III), thereby preventing decay to more stable Co(III) complexes78. Partenheimer speculated that Zr affects the selectivity of toluene oxidation by promoting hydroperoxide coordination and dehydration to benzaldehyde and preventing the precipitation of MnO247,48. While it is unclear whether these models can be extrapolated to our Mn/Zr catalyst and lignin substrate, we did observe an increase in selectivity toward reactions that produce the desired acid and aldehyde products as opposed to deleterious side reactions. It should be noted that Partenheimer also observed a selectivity difference in oxidations of hydroxymethylfurfural. With Zr, double the yield of 2,5-diformylfuran was observed82.
The Br co-catalyst in Co/Mn/Br also differs significantly from our Mn/Zr catalyst. In the former system, Br initiates the radical-chain process by H-atom transfer. In the absence of Br, it has been proposed that Mn(III) generates radicals by single-electron transfer from the electron-rich lignin aromatics via the loss of a proton from a radical cation intermediate83. It has been suggested that Co(III) is generated from Co(II) in Co/O2/AcOH systems by adventitious peroxide84.
Oxidation specifically allows the production of bio-available compounds that can be funnelled into a single product, in this case, cis,cis-muconic acid, a platform chemical used in the production of biopolymers including nylon, nylon derivatives, and polyethylene terephthalate (PET), as well as performance-advantaged composites85,86,87,88,89,90,91,92. Translation of the CYP199A4 system from R. palustris HaA2 provides a catabolic pathway for p-methoxylated aromatics in engineered P. putida, and the use of oxidized RCF oligomers offers an example of biological funnelling of a heterogenous, lignin-derived stream toward a single product. The oxidized RCF oligomer streams were provided to P. putida at dilute concentrations, but the strains rapidly utilized the major monomer constituents (veratrate and veratraldehyde), indicating that tolerance to and conversion of higher substrate loadings is within the performance capability of these strains, as we have demonstrated for similar feedstocks and engineered pathways93,94. Although our system does not produce appreciable monomers from 5-5 dimers, a metabolic pathway for cleavage of the 5-5 C–C bond has been reported95,96,97. The resulting monomers—equivalents of vanillate and 4-carboxy-2-hydroxypenta-2,4-dienoate (CHPD), in the case of 5,5′-dehydrodivanillate cleavage—could then be directed toward central metabolism or engineered pathways for muconate production in P. putida97,98. Going forward, bioprocess development could be combined with metabolic engineering or evolution strategies to improve the performance of these biocatalysts, increasing the yield of muconate or similar products93,94,99,100,101,102 from lignin.
We have demonstrated C–C bond cleavage in lignin using a Mn and Zr catalyst system in acetic acid, as shown with oligomers derived from pine and poplar lignin RCF oils. The method uses molecular oxygen as the oxidant and phenol-stabilization chemistry and yields of 1.01(4) and 0.96(2) mmol of monomers/g of oligomer substrate were obtained for pine and poplar, respectively. The oxygenated and bio-available product stream produced from pine oligomer oxidation was converted in biological systems using P. putida KT2440 to cis,cis-muconate.
Methods
Autoxidation catalysis of the oligomer fractions
For a single reaction, a 75 mL Parr batch reactor fit with a glass liner insert was charged with 20-65 mg of functionalized RCF oil oligomers, acetic acid (15 mL), a stir bar, and Mn(OAc)2•4H2O and Zr(acac)4 catalyst. The mixture was pressurized three times with pure nitrogen and subsequently charged with air and nitrogen to achieve the desired oxygen partial pressure. The vessel was heated to temperature, at which, it was maintained for the desired timeframe before it was cooled rapidly in an ice bath. The resultant homogeneous solutions were stored in the freezer until needed for analysis. For analysis, an aliquot of the autoxidation reaction mixture was taken, and the acetic acid was evaporated under a stream of N2 at room temperature. Dimethylformamide, excess potassium carbonate (10 mass equiv.), and excess methyl iodide (10 mass equiv.) were then added to the residue, and the mixture was stirred at room temperature for 3 h. To the slurry, an internal standard was added (naphthalene), and the mixture was filtered with a 0.2 µm porosity filter frit into an analysis vial before being analyzed using the GC-FID methods described in the Supplementary Information.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
Additional synthetic and spectroscopic details, GC-FID quantification data, and strain and biological conversion details are included in this paper and its Supplementary Information files. Any further information is available from the corresponding author upon request.
References
Zakzeski, J., Bruijnincx, P. C., Jongerius, A. L. & Weckhuysen, B. M. The catalytic valorization of lignin for the production of renewable chemicals. Chem. Rev. 110, 3552–3599 (2010).
Chundawat, S. P., Beckham, G. T., Himmel, M. E. & Dale, B. E. Deconstruction of lignocellulosic biomass to fuels and chemicals. Annu. Rev. Chem. Biomol. Eng. 2, 121–145 (2011).
Li, C., Zhao, X., Wang, A., Huber, G. W. & Zhang, T. Catalytic transformation of lignin for the production of chemicals and fuels. Chem. Rev. 115, 11559–11624 (2015).
Rinaldi, R. et al. Paving the way for lignin valorisation: recent advances in bioengineering, biorefining and catalysis. Angew. Chem. Int. Ed. 55, 8164–8215 (2016).
Sun, Z., Fridrich, B., de Santi, A., Elangovan, S. & Barta, K. Bright side of lignin depolymerization: toward new platform chemicals. Chem. Rev. 118, 614–678 (2018).
Schutyser, W. et al. Chemicals from lignin: an interplay of lignocellulose fractionation, depolymerisation, and upgrading. Chem. Soc. Rev. 47, 852–908 (2018).
Wong, S. S., Shu, R., Zhang, J., Liu, H. & Yan, N. Downstream processing of lignin derived feedstock into end products. Chem. Soc. Rev. 49, 5510–5560 (2020).
Ragauskas, A. J. et al. Lignin valorization: improving lignin processing in the biorefinery. Science 344, 1246843 (2014).
Boerjan, W., Ralph, J. & Baucher, M. Lignin biosynthesis. Annu. Rev. Plant Biol. 54, 519–546 (2003).
Abu-Omar, M. M. et al. Guidelines for performing lignin-first biorefining. Energy Environ. Sci. 14, 262–292 (2021).
Kim, S. et al. Computational study of bond dissociation enthalpies for a large range of native and modified lignins. J. Phys. Chem. Lett. 2, 2846–2852 (2011).
Dong, L. et al. Breaking the limit of lignin monomer production via cleavage of interunit carbon–carbon linkages. Chem. 5, 1521–1536 (2019).
Dong, L. et al. Mechanisms of Caromatic-C bonds cleavage in lignin over NbOx-supported Ru catalyst. J. Catal. 394, 94–103 (2021).
Wang, M. et al. Dealkylation of lignin to phenol via oxidation–hydrogenation strategy. ACS Catal. 8, 6837–6843 (2018).
Yan, J. et al. Selective valorization of lignin to phenol by direct transformation of Csp2–Csp3 and C–O bonds. Sci. Adv. 6, eabd1951 (2020).
Luo, H. et al. Oxidative catalytic fractionation of lignocellulosic biomass under non-alkaline conditions. J. Am. Chem. Soc. 143, 15462–15470 (2021).
Zirbes, M., Graßl, T., Neuber, R. & Waldvogel, S. R. Peroxodicarbonate as a green oxidizer for the selective degradation of kraft lignin into vanillin. Angew. Chem. Int. Ed. 62, e202219217 (2023).
Liu, H., Li, H., Luo, N. & Wang, F. Visible-light-induced oxidative lignin C–C bond cleavage to aldehydes using vanadium catalysts. ACS Catal. 10, 632–643 (2019).
Zhang, C. & Wang, F. Catalytic lignin depolymerization to aromatic chemicals. Acc. Chem. Res. 53, 470–484 (2020).
Xin, Y. et al. Organic amine mediated cleavage of C(aromatic)-C(alpha) bonds in lignin and its platform molecules. Chem. Sci. 12, 15110–15115 (2021).
Hanson, S. K. & Baker, R. T. Knocking on wood: base metal complexes as catalysts for selective oxidation of lignin models and extracts. Acc. Chem. Res. 48, 2037–2048 (2015).
Renders, T., Van den Bosch, S., Koelewijn, S. F., Schutyser, W. & Sels, B. Lignin-first biomass fractionation: the advent of active stabilisation strategies. Energy Environ. Sci. 10, 1551–1557 (2017).
Renders, T., Van den Bossche, G., Vangeel, T., Van Aelst, K. & Sels, B. Reductive catalytic fractionation: state of the art of the lignin-first biorefinery. Curr. Opin. Biotechnol. 56, 193–201 (2019).
Questell-Santiago, Y. M., Galkin, M. V., Barta, K. & Luterbacher, J. S. Stabilization strategies in biomass depolymerization using chemical functionalization. Nat. Rev. Chem. 4, 311–330 (2020).
Subbotina, E. et al. Oxidative cleavage of C-C bonds in lignin. Nat. Chem. 13, 1118–1125 (2021).
Weber, M., Pompetzki, W., Bonmann, R. & Weber, M. Acetone. In: Ullmann’s Encyclopedia of Industrial Chemistry (Wiley, 2014).
Musser, M. T. Cyclohexanol and cyclohexanone. In: Ullmann’s Encyclopedia of Industrial Chemistry (Wiley, 2011).
Partenheimer, W. Methodology and scope of metal/bromide autoxidation of hydrocarbons. Cat. Today 23, 69–158 (1995).
J. Sheehan R. Terephthalic acid, dimethyl terephthalate, and isophthalic acid. In: Ullmann’s Encyclopedia of Industrial Chemistry (Wiley, 2011).
Tomas, R. A., Bordado, J. C. & Gomes, J. F. p-Xylene oxidation to terephthalic acid: a literature review oriented toward process optimization and development. Chem. Rev. 113, 7421–7469 (2013).
Adamian, V. A. & Gong, W. H. In Liquid Phase Aerobic Oxidation Catalysis: Industrial Applications and Academic Perspectives: Industrial Applications and Academic Perspectives. 41–66 (Wiley, 2016).
Stahl, S. S. & Alsters, P. L. Liquid Phase Aerobic Oxidation Catalysis: Industrial Applications and Academic Perspectives. (John Wiley & Sons, 2016).
Jones, F. N. Alkyd resins. In: Ullmann’s Encyclopedia of Industrial Chemistry (Wiley, 2003).
Funke, W. et al. Paints and coatings, 2. Types. In: Ullmann’s Encyclopedia of Industrial Chemistry (Wiley, 2010).
Sheldon, R. A. & Kochi, J. K. In Advances in Catalysis. (Elsevier, 1976).
Partenheimer, W. The unusual characteristics of the aerobic oxidation of 3,4-dimethoxytoluene with metal/bromide catalysts. Adv. Synth. Catal. 346, 1495–1500 (2004).
Partenheimer, W. The aerobic oxidative cleavage of lignin to produce hydroxyaromatic benzaldehydes and carboxylic acids via metal/bromide catalysts in acetic acid/water mixtures. Adv. Synth. Catal. 351, 456–466 (2009).
Sullivan, K. P. et al. Mixed plastics waste valorization through tandem chemical oxidation and biological funneling. Science 378, 207–211 (2022).
Partenheimer, W. Valuable oxygenates by aerobic oxidation of polymers using metal/bromide homogeneous catalysts. Cat. Today 81, 117–135 (2003).
Gu, N. X. et al. Autoxidation catalysis for carbon–carbon bond cleavage in lignin. ACS Cent. Sci. 9, 2277--2285 (2023).
Bystrzanowska, M., Petkov, P. & Tobiszewski, M. Ranking of heterogeneous catalysts metals by their greenness. ACS Sust. Chem. Eng. 7, 18434–18443 (2019).
Linger, J. G. et al. Lignin valorization through integrated biological funneling and chemical catalysis. Proc. Natl. Acad. Sci. USA 111, 12013–12018 (2014).
Erickson, E. et al. Critical enzyme reactions in aromatic catabolism for microbial lignin conversion. Nat. Catal. 5, 86–98 (2022).
Werner, A. Z. & Eltis, L. D. Tandem chemocatalysis and biological funneling to valorize lignin. Trends Biotechnol. 41, P270–272 (2022).
Lee, S. et al. Microbial production of 2-pyrone-4, 6-dicarboxylic acid from lignin derivatives in an engineered Pseudomonas putidaand its application for the synthesis of bio-based polyester. Bioresour. Technol. 352, 127106 (2022).
Vardon, D. R. et al. Adipic acid production from lignin. Energy Environ. Sci. 8, 617–628 (2015).
Partenheimer, W. The effect of zirconium in metal/bromide catalysts during the autoxidation of p-xylene: Part i. Activation and changes in benzaldehyde intermediate formation. J. Mol. Catal. A Chem. 206, 105–119 (2003).
Partenheimer, W. The effect of zirconium in metal/bromide catalysts on the autoxidation of p-xylene. J. Mol. Catal. A Chem. 206, 131–144 (2003).
Jang, J. H. et al. Multi-pass flow-through reductive catalytic fractionation. Joule 6, 1859–1875 (2022).
Beckham, G. T., Johnson, C. W., Karp, E. M., Salvachua, D. & Vardon, D. R. Opportunities and challenges in biological lignin valorization. Curr. Opin. Biotechnol. 42, 40–53 (2016).
Kuatsjah, E. et al. Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate. Metab. Eng. 70, 31–42 (2022).
Bell, S. G., Tan, A. B., Johnson, E. O. & Wong, L. L. Selective oxidative demethylation of veratric acid to vanillic acid by CYP199A4 from Rhodopseudomonas palustris HaA2. Mol. Biosyst. 6, 206–214 (2010).
Providenti, M. A., O’Brien, J. M., Ruff, J., Cook, A. M. & Lambert, I. B. Metabolism of isovanillate, vanillate, and veratrate by Comamonas testosteroni strain BR6020. J. Bacteriol. 188, 3862–3869 (2006).
Jensen, E. D. et al. Integrating continuous hypermutation with high-throughput screening for optimization of cis,cis-muconic acid production in yeast. Microb. Biotechnol. 14, 2617–2626 (2021).
Clatworthy, E. B. et al. Investigating homogeneous Co/Br−/H2O2 catalysed oxidation of lignin model compounds in acetic acid. Catal. Sci. Technol. 9, 384–397 (2019).
Bomben, A., Selva, M., Tundo, P. & Valli, L. A continuous-flow O-methylation of phenols with dimethyl carbonate in a continuously fed stirred tank reactor. Ind. Eng. Chem. Res. 38, 2075–2079 (1999).
Tundo, P. & Selva, M. The chemistry of dimethyl carbonate. Acc. Chem. Res. 35, 706–716 (2002).
Sen, S., Patil, S. & Argyropoulos, D. S. Methylation of softwood kraft lignin with dimethyl carbonate. Green Chem. 17, 1077–1087 (2015).
Dabral, S. et al. Mechanistic studies of base-catalysed lignin depolymerisation in dimethyl carbonate. Green Chem. 20, 170–182 (2018).
Liu, B. et al. Oxygen vacancy promoting dimethyl carbonate synthesis from CO2 and methanol over Zr-doped CeO2 nanorods. ACS Catal. 8, 10446–10456 (2018).
Stoian, D., Medina, F. & Urakawa, A. Improving the stability of CeO2 catalyst by rare earth metal promotion and molecular insights in the dimethyl carbonate synthesis from CO2 and methanol with 2-cyanopyridine. ACS Catal. 8, 3181–3193 (2018).
Lee, K. M. et al. Redox-neutral electrochemical conversion of CO2 to dimethyl carbonate. Nat. Energy 6, 733–741 (2021).
Ito, Y. Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography. J. Chromatogr. A 1065, 145–168 (2005).
Mota, M. I. F., Rodrigues Pinto, P. C., Loureiro, J. M. & Reodrigues, A. Recovery of vanillin and syringaldehyde from lignin oxidation: a review of separation and purification processes. Sep. Purif. Rev. 45, 227–259 (2016).
Alherech, M. et al. From lignin to valuable aromatic chemicals: lignin depolymerization and monomer separation via centrifugal partition chromatography. ACS Cent. Sci. 7, 1831–1837 (2021).
Bartling, A. W. et al. Techno-economic analysis and life cycle assessment of a biorefinery utilizing reductive catalytic fractionation. Energy Environ. Sci. 14, 4147–4168 (2021).
Steinmetz, G. R. & Sumner, C. E. Role of amine promoters in cobalt/bromide-catalyzed oxidations. J. Catal. 100, 549–551 (1986).
Steinmetz, G., Lafferty, N. & Sumner, C. Jr. The cobalt/zirconium-catalyzed oxidation of cyclohexane to adipic acid. J. Mol. Catal. 49, L39–L42 (1988).
Baxendale, J. & Wells, C. The reactions of Co (III) with water and with hydrogen peroxide. Trans. Faraday Soc. 53, 800–812 (1957).
Hendriks, C. F., van Beek, H. C. & Heertjes, P. M. Reactions of some peracids and hydroperoxides with cobalt (II) and cobalt (III) acetate in acetic acid solution. Ind. Eng. Chem. Prod. Res. Dev. 18, 38–43 (1979).
Sumner, C. E. Jr. Interconversion of dinuclear and oxo-centered trinuclear cobaltic acetates. Inorg. Chem. 27, 1320–1327 (1988).
Sumner, C. E. Jr. & Steinmetz, G. R. Isolation of oxo-centered cobalt (III) clusters and their role in the cobalt bromide catalyzed autoxidation of aromatic hydrocarbons. J. Am. Chem. Soc. 107, 6124–6126 (1985).
Chavan, S., Halligudi, S., Srinivas, D. & Ratnasamy, P. Formation and role of cobalt and manganese cluster complexes in the oxidation of p-xylene. J. Mol. Catal. A Chem. 161, 49–64 (2000).
Babushkin, D. & Talsi, E. Multinuclear NMR spectroscopic characterization of Co (III) species: Key intermediates of cobalt catalyzed autoxidation. J. Mol. Catal. A Chem. 130, 131–137 (1998).
Jones, G. H. p-xylene autoxidation studies. Oxidation of cobalt (II) and manganese (II) acetates by peracids. J. Chem. Soc. Chem. Commun. 1979, 536–537 (1979).
Lande, S. S., Falk, C. D. & Kochi, J. K. Cobalt (III) acetate from the ozonation of cobaltous acetate. J. Inorg. Nuc. Chem. 33, 4101–4109 (1971).
Jiao, X.-D. & Espenson, J. H. Kinetics of manganese (III) acetate in acetic acid: Generation of Mn (III) with Co (III), Ce (IV), and dibromide radicals; reactions of Mn (III) with Mn (II), Co (II), hydrogen bromide, and alkali bromides. Inorg. Chem. 39, 1549–1554 (2000).
Chester, A. W., Scott, E. J. & Landis, P. S. Zirconium cocatalysis of the cobalt-catalyzed autoxidation of alkylaromatic hydrocarbons. J. Catal. 46, 308–319 (1977).
Scott, E. & Chester, A. Kinetics of the cobalt-catalyzed autoxidation of toluene in acetic acid. Role of cobalt. J. Phys. Chem. 76, 1520–1524 (1972).
Blake, A. B., Chipperfield, J. R., Lau, S. & Webster, D. E. Studies on the nature of cobalt (III) acetate. J. Chem. Soc. Dalton Trans. 1990, 3719–3724 (1990).
Chipperfield, J. R., Lau, S. & Webster, D. E. Speciation in cobalt (III)-catalysed autoxidation: Studies using thin-layer chromatography. J. Mol. Catal. 75, 123–128 (1992).
Partenheimer, W. & Grushin, V. V. Synthesis of 2, 5‐diformylfuran and furan‐2, 5‐dicarboxylic acid by catalytic air‐oxidation of 5‐hydroxymethylfurfural. unexpectedly selective aerobic oxidation of benzyl alcohol to benzaldehyde with metal=bromide catalysts. Adv. Synth. Catal. 343, 102–111 (2001).
DiCosimo, R. & Szabo, H. C. Oxidation of lignin model compounds using single-electron-transfer catalysts. J. Org. Chem. 53, 1673–1679 (1988).
Partenheimer, W. Characterization of the reaction of cobalt (II) acetate, dioxygen and acetic acid, and its significance in autoxidation reactions. J. Mol. Catal. 67, 35–46 (1991).
Frost, J. W. & Draths, K. M. Synthesis of adipic acid from biomass-derived carbon sources. U.S. Patent No. 5, 487, 987 (1996).
Snell, K., Draths, K. & Frost, J. Synthetic modification of the Escherichia coli chromosome: enhancing the biocatalytic conversion of glucose into aromatic chemicals. J. Am. Chem. Soc. 118, 5605–5614 (1996).
Frost, J. W. & Draths, K. M. Bacterial cell tranformants for production of cis, cis-muconic acid and catechol. U.S. Patent No. 5,616,496 (1997).
Frost, J. W., Miermont, A., Schweitzer, D., Bui, V. & Wicks, D. A. Terephthalic and trimellitic based acids and carboxylate derivatives thereof. U.S. Patent US8367858 B2 (2013).
Lu, R. et al. Production of diethyl terephthalate from biomass-derived muconic acid. Angew. Chem. Int. Ed. 55, 249–253 (2016).
Rorrer, N. A. et al. Renewable unsaturated polyesters from muconic acid. ACS Sust. Chem. Eng. 4, 6867–6876 (2016).
Rorrer, N. A., Vardon, D. R., Dorgan, J. R., Gjersing, E. J. & Beckham, G. T. Biomass-derived monomers for performance-differentiated fiber reinforced polymer composites. Green Chem. 19, 2812–2825 (2017).
Shanks, B. H. & Keeling, P. L. Bioprivileged molecules: creating value from biomass. Green Chem. 19, 3177–3185 (2017).
Salvachúa, D. et al. Bioprocess development for muconic acid production from aromatic compounds and lignin. Green Chem. 20, 5007–5019 (2018).
Elmore, J. R. et al. Engineered Pseudomonas putida simultaneously catabolizes five major components of corn stover lignocellulose: glucose, xylose, arabinose, p-coumaric acid, and acetic acid. Metab. Eng. 62, 62–71 (2020).
Masai, E., Katayama, Y., Nishikawa, S. & Fukuda, M. Characterization of Sphingomonas paucimobilis SYK-6 genes involved in degradation of lignin-related compounds. J. Ind. Microbiol. Biotechnol. 23, 364–373 (1999).
Peng, X., Masai, E., Katayama, Y. & Fukuda, M. Characterization of the meta-cleavage compound hydrolase gene involved in degradation of the lignin-related biphenyl structure by Sphingomonas paucimobilis SYK-6. Appl. Environ. Microbiol. 65, 2789–2793 (1999).
Kamimura, N. et al. Bacterial catabolism of lignin-derived aromatics: New findings in a recent decade: Update on bacterial lignin catabolism. Environ. Microbiol. Rep. 9, 679–705 (2017).
Mori, K., Niinuma, K., Fujita, M., Kamimura, N. & Masai, E. DdvK, a novel major facilitator superfamily transporter essential for 5,5’-dehydrodivanillate uptake by Sphingobiumsp. strain SYK-6. Appl. Environ. Microbiol. 84, e01314–e01318 (2018).
Johnson, C. W. et al. Innovative chemicals and materials from bacterial aromatic catabolic pathways. Joule 3, 1523–1537 (2019).
Xu, Z., Lei, P., Zhai, R., Wen, Z. & Jin, M. Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products. Biotechnol. Biofuels 12, 32 (2019).
Yaguchi, A. L., Lee, S. J. & Blenner, M. A. Synthetic biology towards engineering microbial lignin biotransformation. Trends Biotechnol. 39, 1037–1064 (2021).
Weiland, F., Kohlstedt, M. & Wittmann, C. Guiding stars to the field of dreams: Metabolically engineered pathways and microbial platforms for a sustainable lignin-based industry. Metab. Eng. 71, 13–41 (2022).
Acknowledgements
This work was authored in part by the National Renewable Energy Laboratory, operated by Alliance for Sustainable Energy, LLC, for the U.S. Department of Energy (DOE) under Contract No. DE-AC36-08GO28308. Funding to C.T.P., K.P.S., S.J.H., K.J.R., L.D.S., D.G.B., J.B., and G.T.B. was provided by the U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Bioenergy Technologies Office. Funding to N.X.G. and A.C.B. was provided by The Center for Bioenergy Innovation, a U.S. DOE Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Contributions by S.S.S. were supported by the US Department of Energy, Office of Basic Energy Sciences, under award no. DEFG02-05ER15690. The views expressed in the article do not necessarily represent the views of the DOE or the U.S. Government. The U.S. Government retains and the publisher, by accepting the article for publication, acknowledges that the U.S. Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this work or allow others to do so, for U.S. Government purposes. We thank David G. Brandner, Jeremy R. Bussard, and Justin B. Sluiter for providing extractives-free pine and poplar RCF oil and Mikhail O. Konev for helpful discussions.
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Conceptualization, C.T.P., N.X.G., A.C.B., K.P.S., S.S.S., G.T.B.; data curation, C.T.P., A.C.B.; formal analysis, C.T.P., A.C.B., M.A.I., K.J.R., S.J.H.; funding acquisition, G.T.B., S.S.S.; investigation and methodology, C.T.P., N.X.G., A.C.B., K.P.S., J.K.K., R.K., L.M.S., C.R.A., S.S.S., G.T.B.; supervision, G.T.B., S.S.S.; visualization, C.T.P.; writing original draft, C.T.P., G.T.B.; review and editing, all authors.
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C.T.P., N.X.G., K.P.S, S.S.S., and G.T.B. have filed a patent application on the catalysis presented in the work. A.C.B., C.R.A., and G.T.B. have also filed a patent application on the P. putida strains presented in this work. The remaining authors declare no competing interests.
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Palumbo, C.T., Gu, N.X., Bleem, A.C. et al. Catalytic carbon–carbon bond cleavage in lignin via manganese–zirconium-mediated autoxidation. Nat Commun 15, 862 (2024). https://doi.org/10.1038/s41467-024-45038-z
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DOI: https://doi.org/10.1038/s41467-024-45038-z
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