Metabolic engineering

Definition

Metabolic engineering is the use of genetic engineering to modify the metabolism of an organism. It can involve the optimization of existing biochemical pathways or the introduction of pathway components, most commonly in bacteria, yeast or plants, with the goal of high-yield production of specific metabolites for medicine or biotechnology.

Latest Research and Reviews

  • Research | | open

    Production of chemicals by microbial fermentation is an economical alternative to chemical synthesis. Here the authors re-engineer the yeast S. cerevisiae to produce the very long chain fatty alcohol docosanol by expressing a heterologous Mycobacteria fatty acid synthase and a specific fatty acid reductase.

    • Tao Yu
    • , Yongjin J. Zhou
    • , Leonie Wenning
    • , Quanli Liu
    • , Anastasia Krivoruchko
    • , Verena Siewers
    • , Jens Nielsen
    •  & Florian David
  • Research |

    A synthetic biology system composed of light-wavelength-responsive genetic regulators, signal-processing circuits and pigment-production pathways have resulted in an Escherichia coli strain that can record color images in RGB format.

    • Jesus Fernandez-Rodriguez
    • , Felix Moser
    • , Miryoung Song
    •  & Christopher A Voigt
  • Research | | open

    Filamentous fungi are a valuable source of natural therapeutic products such as antibiotics. Here the authors engineer monocellular S. cerevisiae to perform complex secondary metabolism typical of multicellular fungi in order to demonstrate biosynthesis and secretion of bioactive penicillin.

    • Ali R. Awan
    • , Benjamin A. Blount
    • , David J. Bell
    • , William M. Shaw
    • , Jack C.H. Ho
    • , Robert M. McKiernan
    •  & Tom Ellis
  • Research | | open

    Corynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida.

    • Yu Jiang
    • , Fenghui Qian
    • , Junjie Yang
    • , Yingmiao Liu
    • , Feng Dong
    • , Chongmao Xu
    • , Bingbing Sun
    • , Biao Chen
    • , Xiaoshu Xu
    • , Yan Li
    • , Renxiao Wang
    •  & Sheng Yang
  • Research | | open

    Genome-scale engineering is a powerful technique for understanding biology and designing microorganisms but has been limited to bacterial species. Here the authors present an automated platform for genome-scale engineering in Saccharomyces cerevisiae using CRISPR-Cas and RNAi.

    • Tong Si
    • , Ran Chao
    • , Yuhao Min
    • , Yuying Wu
    • , Wen Ren
    •  & Huimin Zhao

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