Abstract
The living supramolecular polymerization technique provides an exciting research avenue. However, in comparison with the thermodynamic spontaneous nucleation, using simple monomers to realize living supramolecular polymerization is hardly possible from an energy principle. This is because the activation barrier of kinetically trapped simple monomer (nucleation step) is insufficiently high to control the kinetics of subsequent elongation. Here, with the benefit of the confinement from the layered double hydroxide (LDH) nanomaterial, various simple monomers, (such as benzene, naphthalene and pyrene derivatives) successfully form living supramolecular polymer (LSP) with length control and narrow dispersity. The degree of polymerization can reach ~6000. Kinetics studies reveal LDH overcomes a huge energy barrier to inhibit undesired spontaneous nucleation of monomers and disassembly of metastable states. The universality of this strategy will usher exploration into other multifunctional molecules and promote the development of functional LSP.
Similar content being viewed by others
Introduction
Supramolecular polymers present us with a broad blueprint to struggle for the new generation of functional materials such as soft matter electronics, drug delivery, and catalysts in a relatively simple way1,2,3,4,5,6,7. Especially, the emergence of the living supramolecular polymerization (LSP) method provides an exciting and novel research avenue, which has been applied to establish supramolecular polymers with efficient modulating and uniformity of chain growth and dispersity8,9. To call a “living”, in the strict sense, the growth of supramolecular polymers from the active ends should be demonstrated by multicycle experiments10,11,12,13,14. In 2014, Sugiyasu and Takeuchi’s15 group established the first seeded LSP via off-pathway aggregates. Inspired by this, several clever strategies were developed to encode the molecular information to achieve programmable LSP16,17,18. However, the further requirement for LSP systems is precise control over the degree of polymerization, chain stereochemistry, and lifetime19. Thus, in 2015, Aida and Miyajima’s group first introduced the chain-growth mechanism to realize the initiator-controlled LSP20. This unique LSP provoked a paradigm shift in precision macromolecular engineering21,22. From the development milestones, LSP is still in its infant stage, and its ultimate goal is to acquire controlled life-like active materials23,24,25. In 2018, Balasubramanian and George’s group demonstrated the first biomimetic LSP by consuming the chemical fuel ATP26. With the benefit of this strategy, various analogs of biological self-assembly motifs have been developed and bring us closer to complex biological entities. Even at this stage, scientists are still confronted with a severe challenge on the monomer design, because the aforementioned works are directed to specific systems, which require painstaking regulation and multi-step modification of the monomer structure27,28,29. This problem inevitably increases the synthetic difficulty and the material cost, greatly limiting their universality and application. To breakthrough the current bottleneck, it is an effective way to fabricate LSPs using simple and commercially available monomers. Nevertheless, this design strategy is hardly possible to succeed from an energy principle, because the activation barrier of simple monomers in the nucleation step is insufficiently high in comparison with that of the spontaneous nucleation to control the kinetics of subsequent elongation15. Therefore, the key to solving this problem lies in finding suitable synthesis methodologies to rationally select assembly pathways, so as to bring the supramolecular polymer with simple monomers to life.
The confinement space that can impact all chemical events taking place in a small cavity has been well documented in the field of nanoreactors, biosensors, and drug delivery vehicles, leading to a contrasting outcome than in the bulk30,31. In terms of self-assembly, there is evidence that confinement space can promote the formation and stability of self-assembled complexes held together by intermolecular interactions32,33,34,35,36,37. For example, in the cell-confined environment, the assembly and folding speed of the polypeptide chain is significantly accelerated38. In the molecular chaperone confined nano-cage, abnormal folding, and aggregation of protein can be inhibited, which in turn promotes the folding rate of normal proteins39,40. In the membrane-compartmentalized confinement environment, actin filaments, and cell microtubules are regulated by a finely tuned, highly complex molecular machinery41. These biological confinement phenomena have provided important clues to guiding the ordered assembly of simple monomers through the confinement approaches to strengthen the intermolecular interactions, so as to achieve the LSP dominated by kinetics control.
In this work, to validate our hypothesis, the confinement effect of the layered double hydroxide (LDH) nanomaterial is tried to guide the assembly of simple monomers by intercalating method42,43,44. Theoretically, after the removal of LDHs template, the ordered assembly of guests will be destroyed because it has been reported that the activation barrier (30 kcal mol−1) for a similar process, spontaneous initiation of styrene polymerization40, is far beyond the energies associated with non-covalent bond formation. However, here, an unexpected phenomenon is discovered that the long-range anisotropic structure (metastable state) assembled by simple monomers cannot only be preserved after dissolving of LDH, but also elongate successfully upon addition of suitable solution. Kinetics and mechanistic studies reveal LDH overcomes a huge energy barrier to inhibit spontaneous nucleation of simple monomers and disassembly of metastable states, which provided the prerequisites of living polymerization. Owing to the living ends, two types of chiral monomers are added in growth steps to form block copolymer, by which the naked eye visualization of chiral recognition can be realized.
Results and discussion
Monomer selection
Here, 8-hydroxypyrene-1,3,6-trisulfonate (solvent green 7, SG7) is chosen as a model to study the formation of LSP because it possesses a pyrene molecule bearing hydrogen-bonding moieties and negative charges, which is expected to self-assemble via π–π stacking of the pyrene planes and hydrogen bond (H-bond) of sulfonyl group. Importantly, there exist remarkable different fluorescence (FL) properties between monomer and various stacking states (Supplementary Figs. 1–2 for a detailed analysis), providing the prerequisite for naked eye visualization of assembly events.
Growth process of supramolecular polymers
Figure 1a depicts the formation process of LSP and seed-induced supramolecular polymer (SSP) from SG7 monomer. Upon intercalating SG7 monomer into the confinement space of LDH, named as SG7-LDH, which is confirmed by powder X-ray diffraction (XRD) measurements (step 1, Supplementary Fig. 3). The oriented arrangement of SG7 in the interlayer of LDH was achieved, as confirmed by FL anisotropy, where SG7-LDH showed a high anisotropic value (r = 0.565) compared with untreated SG7 powder (r = 0.0201) (Supplementary Figs. 4–5). The ab initio molecular dynamics (AIMD) simulations further testified the ordered SG7 with an orientation angle of θ = 9° (θ is the inclined angle between the principal axis of molecule and the elongation axis of oriented arrangement, Supplementary Fig. 6). After removal of LDH by using methanol/trifluoroacetic acid (CH3OH/TFA) mixture solvent (5:3 v/v), the pre-assembly as the seed is obtained, which is a metastable and living supramonomer (LSM) (step 2). As confirmed by FL spectra and photos under UV light (Fig. 1b−c, Supplementary Figs. 7–8), compared with SG7 monomer (λem = 430 nm), the pre-assembly LSM (λem = 460 nm) showed red-shifted wavelength, indicating emergence of J-aggregate among LSM; compared with SG7-LDHs (λem = 590 nm), the pre-assembly LSM (λem = 460 nm) showed a blue shift attributed to shorter J-aggregate. On the other hand, the metastable properties of LSM can be proved by that LSM can maintain for ~10 min and spontaneously convert into the equilibrium state over time (detailed in Supplementary Fig. 9); living properties can be proved by that LSM can continue to grow to metastable LSP initiated by increasing poor solvents (TFA) until CH3OH/TFA (1:3 v/v), that is, the step 3. This growth shows self-replication characteristic and formation of longer J-aggregate, confirmed by the nonlinear sigmoidal increase in temperature-dependent absorption (Supplementary Fig. 10) and emission redshift from 460 to 535 nm (Fig. 1c, Supplementary Figs. 8 and 11). As shown in the FL intensity time scan (FITS), the metastable properties of LSP can be proved by its constant FL intensity and wavelength in the first 5 min (Supplementary Figs. 12–13). The wide excitation range of LSP is also in line with the long-range π–π stacking rather than a single molecule (Supplementary Fig. 14). Step 4 is an energetically favored elongation step of metastable LSP. As confirmed by scanning electron microscopy (SEM) images and FITS (Fig. 1e−f and Supplementary Figs. 12 and 15), elongated LSP showed increased size and decreased FL intensity over time. Compared with metastable LSP, decreased FL intensity of elongated LSP in FL emission spectra (Supplementary Fig. 12) is attributed to the FL quenching by elongation; unchanged wavelength (λem = 535 nm) is attributed to the maintenance of J-aggregate. Step 5 is a seed-induced living polymerization by adding inactive SG7agg (SG7 dissolved in CH3OH/TFA (1:3 v/v), 2.5 mM) into metastable LSP (LSPseed) to form SSP. In FL emission spectra (Supplementary Figs. 16–18), the formed SSP (λem = 525 nm) shows redshift compared with SG7agg (λem = 450 nm), indicating the rearrangement of SG7agg induced by LSPseed. In addition, SSP shows an emission color change and blue shift of 10 nm compared with LSP (Fig. 1d), attributing to the tiny difference in newly formed J-aggregate compared with original LSPseed. In SEM images (Fig. 1g–h and Supplementary Fig. 19), the transformation of morphology from spherical (SG7agg) to rectangular (SSP) and increased size of SSP in cycle experiments also confirm the living polymerization induced by LSP. In addition, this system is reversible and can be prepared in low concentration (detailed in Supplementary Figs. 20–24). None of the products is crystal (Supplementary Fig. 25). The above results fully prove that this assembly process belongs to supramolecular polymerization.
It is worth mentioning that the SG7 is not only intercalated in the cavity of LDH, but also adsorbed on the outer surface of the LDH. To quantitatively describe the effect of SG7 adsorption on assembly, we employed the CO3-LDH20 (the interlayer anions of LDH is CO32−) to adsorb SG7 (marked as SG7-LDH20-surface, detailed in supplementary information), where the intercalation does not occur because the affinity between CO32− and LDH layer is much greater than that between organic simple molecules and LDH layer45,46. ICP-MS and elemental analysis results show that the adsorption capacity is very low and only accounts for 13.41% of the total SG7 (Supplementary Table 1–2). The polarized FL profiles in Supplementary Figs. 4 and 26, SG7-LDH20-surface (r = 0.171) shows much lower anisotropic value than that of SG7-LDH20 (r = 0.565). In addition, the adsorbed SG7 did not assemble into LSP, as confirmed by SEM images and FL spectrum. SEM showed that a SP with rectangular morphology can be obtained, but it fails to induce following cycle products (Supplementary Fig. 27), indicating that SP20 is not a kind of LSP. Instead, the appearance of depolymerization of SP20 in cycle process, as certified by decreased FL intensity of cycle products of SP20 (Supplementary Fig. 28). To sum up, the adsorbed SG7 did not assemble into LSP owing to lack of ordered arrangement. Importantly, the appearance of adsorbed SG7 did not affect the formation of LSP, as proved by physically mixing SG7-LDH20-surface and SG7-LDH to perform cycle experiments. The cycle products showed rectangular morphology with increased size area in SEM and FL intensity, indicating the cycle products have the ability for living assembly (Supplementary Figs. 27–28).
Generally, the presence of inorganic ions during the assembly process will affect the result of the assembly47,48. In our system, in the process of dissolving LDH, metal ions (Mg2+ and Al3+) are introduced. In order to eliminate their influence, we designed a series of comparative samples treated by the same process as an assembly of SG7-LDH, including SG7 powder, Cl-LDH, and SG7+LDH (physically mixed SG7 and Cl-LDH). Both SG7 and SG7+LDH can only form isotropic spherical structures as shown in SEM image (Supplementary Fig. 29a, c). For Cl-LDH, SEM image shows irregular morphology of metal salt (Supplementary Fig. 29b). These results indicate that the ion of dissolved LDH is not the key to facilitate rectangular morphology of LSP.
Relationship between outcome and LDH confinement effect
According to the properties of the kinetic and non-equilibrium system, the formation pathway and outcome of metastable LSP should be controlled by different preparation protocols11. Thus, we tried to change the confinement size by tuning LDH size. As the size of LDH has great controllability and will not be significantly affected by the intercalation process of SG7 (Supplementary Figs. 30–31), it provides the prerequisite for the size regulation of SG7 LSM49,50. It should be pointed out that for nanoscale structures, aggregation cannot be completely avoided51,52. Thus, in order to observe accurately, we etch part of the LDH to expose the metastable LSM before aggregation, that is, the metastable LSM is still on the etched fragments of LDH. As confirmed by SEM images (Fig. 2a–d and their insets), the sizes of metastable LSMs gradually increase with the raising of LDH sizes from 20 nm to 3 μm (as-prepared LSMs from different size LDHs are named as metastable LSM20, LSM50, LSM100, and LSM3000, respectively). Note that, metastable LSMs easily aggregate to its stable state confirmed by SEM, preventing following transformation to LSP (Supplementary Figs. 9 and 32). Thus, the increase of poor solvent TFA can initiate metastable LSMs to continue to grow into metastable LSPs (named as metastable LSP20, LSP50, LSP100, and LSP3000, respectively). The highly ordered structure of metastable LSP20−LSP3000 can be confirmed by high FL anisotropic values (r > 0.45, Supplementary Fig. 33). As shown in SEM images, the sizes from metastable LSP20 to LSP3000 increase sequentially (Supplementary Fig. 34). During their formation, the decline rate of FL intensity and redshift are different from each other, indicating that the kinetics of transformation depends on confinement space owing to pathway complexity (Supplementary Fig. 35). The pathway not only influenced the rate of primary nucleation of metastable LSP but also in energy, confirmed by their different emission wavelength at 525, 510, 516, and 523 nm, respectively) (λex = 370 nm, Supplementary Figs. 35–36). In addition, metastable LSP20−LSP3000 had different activity in elongation during the relaxation to the energetically favored state, confirmed by the different area of elongated LSP20−LSP3000 (1.50, 0.32, 2.0, and 4.0 μm2 in Fig. 2e–h). The activity of metastable LSP will be discussed in the following cycle experiments.
To evaluate the polydispersity index (PDI) and degree of polymerization (DP), we, respectively, studied SEM images and static light scattering of metastable LSP in three replicate experiments (Fig. 2i–l and Supplementary Fig. 34). The number-average area (An), weight-average area (Aw), and PDI (Aw/An) of LSP20−LSP3000 were obtained by evaluating over 50 objects in SEM images (detailed in Supplementary Table 3). According to Rayleigh equation53, the DP of the metastable LSP20, LSP50, LSP100, and LSP3000 is ~40, 400, 4000, and 6000 (Supplementary Table 4–5), respectively. Considering the size extension over time (Supplementary Fig. 15), in fact, the DP of ultimately obtained LSP is much higher than these values. Compared with previous reports, the DP of LSP proposed in our work has made an exciting breakthrough (Supplementary Table 6).
Relationship between outcome and formation pathway
In addition, the conventional preparation protocol have also been employed to testify the pathway selection of the metastable LSPs (taking the LSP20 an example) by changing experimental parameters, such as timing of addition of the bad solvent and mechanical agitation.
The size of metastable LSP20 is dependent on the timing of addition of TFA to SG7-LDH (4 mg in 200 μL CH3OH) during self-assembly. In detail, Condition (1) 0 min with 600 μL; Condition (2) 0 min with 200 μL and 30 min with 400 μL; Condition (3) 0 min with 120 μL and 30 min 480 μL; Condition (4) 0 min with 120 μL and 24 h with 480 μL (Fig. 3a). As seen in the time-dependent FL spectra (detailed in Supplementary Figs. 9, 35–37), metastable LSP20 in Condition (1–3) appeared at 20 min, 45 min, and 48 min, respectively, indicating the decreased rate of nucleation, and consequently its rate. SEM images showed the decreased size of metastable LSP20 (0.04 μm2, 0.006 μm2, and 0.0036 μm2, Supplementary Fig. 38a−c)). In condition (4), metastable LSM20 failed to transform to LSP20 because it has access to its energetically favored state before being kept in CH3OH/TFA (1:3 v/v) (Supplementary Figs. 9 and 38d). Thus, structurally different metastable assemblies under the same final conditions can be obtained by changing the timing of the addition of TFA.
The size of metastable LSP20 is also influenced by mechanical agitation. As seen in confocal laser scanning microscope image, metastable LSP20 without ultrasound move vigorously before 10 min, but severe stack can be observed as time increase (Supplementary Figs. 39–40 and Movie 1). In contrast, mechanical agitation can create smaller structures with fully exposed active sites as initiators for living polymerization. When LSM20 is sonicated for 1 h, the as-obtained product (metastable LSP20) shows uniform distribution of area (Fig. 3b). After being kept for 12 h, kinetics capture extends the area from 0.03 μm2 to 1.50 μm2 (Supplementary Fig. 15), while retaining uniform characteristics (Fig. 3c). Importantly, a competitive growth of elongated LSP20 (12 h) at anisotropic active sites can be observed. The average width increased by 200%, whereas the length only increased by 48% by prolonging ultrasound time (Fig. 3d and Supplementary Fig. 41).
The kinetics behavior of SSP
The characteristic kinetics evolution of metastable LSP20 offers the possibility of acting as reactive seeds (defined as LSPseed) to provoke a rapid growth or transformation of SG7agg to be SSP. Figure 4a−h and Supplementary Figs. 42–44 strongly suggest that the transformation from spherical inactivated SG7agg to rectangular SSP can be initiated by the addition of “seeds” (LSPseed, step 5 in Fig. 1). Previous seed-induced polymerization acquired mixing diluted monomer stock solution with a seed suspension to prevent spontaneous aggregation of the monomer54,55,56. In contrast to these protocols, SG7agg in our system is inactivated spherical aggregates (Supplementary Fig. 29), which can join in the formation of SSP owing to the “infection” of LSPseed. Compared with the metastable LSP50−LSP3000, metastable LSP20 is the most superior in seeds experiment because SSP20 shows regular increased size in cycle experiments (Supplementary Fig. 19), whereas SSP50−SSP3000 split into small fragments (Supplementary Fig. 45).
During the seed-induced polymerization, the area of SSP20 can be controlled by regulating SG7agg concentration (Fig. 4a) or the ratio of SG7agg to LSPseed (Fig. 4b). The maximum area of SSP20 can reach ~1.95 μm2 (Supplementary Figs. 42–43). Similarly, the SSP20 growth can also be controlled by changing the concentration of LSPseed, which is realized by adjusting the initial concentration of SG7-LDH (Fig. 4c). The area of fresh SSP20 can reach ~2.22 μm2 (Supplementary Fig. 44). This kind of growth characteristics related to initial feed parameters has been observed experimentally by Kazunori Sugiyasu et al. in their study of regulating SSP through self-assembly of porphyrin derivative57.
During the living elongation process, the replicator r can be calculated by Eq. (1). Where F is a food molecule, R is a replicator, kR is the rate constant, f and r are the order of the replication process in food and replicator, respectively.
As the concentration of LSPseed increases from 0.250 to 2.50 mM, the time of LSPseed beginning to grow decreases from 800 to 5 s (Fig. 4d). Following the addition of SG7agg to different amounts of LSPseed, polymerization proceeded linearly with respect to the reaction time, confirming the living elongation nature (Fig. 4e). The logarithm of the apparent polymerization rate of SSP, log(d(Abs460)/dt), is proportional to the logarithm of LSPseed concentration, log([seeds]), with a slope of 1.058 (Fig. 4e). Thus, within the experimental error, the polymerization reaction is close to first order and appears to be capable of exponential replication, which is one of the characteristics of living polymerization58.
Living supramolecular polymerization
The FL and SEM spectra were also used to monitor the living nature of seed-induced polymerization (Fig. 4f−h). The metastable LSP20 and fresh SG7agg were freshly prepared and mixed in equal volumes, followed with ultrasound for 1 h. In Cycle 1, after two kinds of solutions were mixed, the blue light attributed to SG7agg disappeared immediately, and the yellow light attributed to SSP20 appeared (Fig. 4g). Further addition of the SG7agg stock solution to the resulting solution repeats the polymerization growth (1:1 v/v, Cycle 2 and 3), but with slower rates. This is consistent with the fact that the initial concentration of LSPseed is diluted by half in every cycle. In Cycle 2, it took 30 min for blue light to disappear. In cycle 3, the blue light did not completely disappear until it is extended for 60 min. In general, as living polymerization proceeds, concentrations of LSPseed and SG7agg decrease, and energy barrier of SSP20 increases, leading to no longer negligible depolymerization (Fig. 4f). Therefore, the area of freshly prepared SSP20 in Cycle 1 reaches 0.54 μm2, while the length of final elongated SSP20 shows unobvious change after Cycle 2 and 3 (Fig. 4h). Unexpectedly, this growth is directional-selective with a large increase in width from 300 to 600 nm (Fig. 4h), indicating highly ordered LSPseed shows fantastic directional rearrangement effect on SG7agg. This can be further testified by FL intensity increase and blue shift of SSP (Fig. 4g) with the increase of cycle index owing to the change of stacking direction from along the long axis to the short axis. Oversized metastable LSP with highly ordered structure has an indispensable role in the living polymerization, which solves the problem of the insufficient energetic barrier by jumping across the spontaneous nucleation. This unusual growth mode gives LSP a great advantage in terms of area compared with previous SSP reports (Supplementary Table 6).
The energy diagram of the growth process
The above experiments have fully proved the mechanism of the transformation from metastable LSM to LSP to be energetically favored. This beneficial elongation behavior derives from the huge energy barrier overcome by LDH, confirmed by theoretical calculation and optical information. As proved by theoretical calculation (Supplementary Fig. 46, detailed information for model construction is listed in the methods section), the locations of global minima and local minima are independent of the number of SG7 molecules, the energies of SG7 monomer, SG7agg and ordered SG7 in LDH can be calculated and compared by using the representative models composed of eight SG7 molecules, as displayed in Fig. 4i−j. The energy of SG7 monomer is calculated to be 167.02 kJ mol−1 higher than that of SG7agg and 126.92 kJ mol−1 higher than that of ordered SG7 in LDH (Supplementary Fig. 46). The driving force of incorporating SG7 into LDH is mainly Coulomb force and H-bond. Thus, the energy level of SG7 solution should be higher than that of thermodynamic equilibrium SG7agg and ordered SG7 in LDH. In addition, the conversion from SG7agg (θ = 35°) to ordered SG7 in LDH (θ = 9°) needs to overcome an energy barrier of 70.55 kJ mol−1 at θ = 18° (the maximum in potential energy surface from 35° to 9°). Thus, ordered SG7 in LDH (θ = 9°) is kept in an energy well to be a metastable state.
With the increased number of SG7 molecules, calculating the energy level of the following transformation needs unaffordable computational cost, which is beyond the state of art. Thus, the schematic illustration for the energy level of metastable LSM, metastable LSP, elongated LSP, and SSP is presented according to the optical information (Fig. 4k). During the transformation, metastable LSP (λem = 525 nm) has lower energy than metastable LSM (λem = 470 nm), confirmed by the redshift in Supplementary Fig. 35. After then, the energy of elongated LSP and SSP are lower than that of metastable LSP owing to the energetically favored elongation step. The decreased emission intensity and redshift of LSP absorbance demonstrate the augment of J-agg owing to the formation of huger H-bond net (Supplementary Figs. 12 and 47–49). The H-bond net can stabilize LSP structure confirmed by the Fourier-transform infrared spectra (Supplementary Figs. 50–53) and solvent-related depolymerization behavior (Supplementary Fig. 54). The energy of the SSP is slightly higher than elongated LSP (step 5), confirmed by a blue shift (Δλ = 10 nm) of FL spectra (Supplementary Figs. 11 and 16).
Application in chiral recognition
The different affinity of chiral molecules during the supramolecular polymerization process endows metastable LSM the possibility of chiral recognition59,60,61. Based on the relationship between structure and luminescence property, the naked eye visual recognition can be realized by the lag time of the chiral recognition products (named as LSM+L or LSM+D). The addition of l- or d-arginine (l- or d-Arg) during the transformation from metastable LSM3000 to LSP leads to the formation of corresponding polymers (defined as polymer-L3000 and polymer-D3000), which show a significant difference in the lag time (Fig. 5a−b). The lag time required for the formation of polymer-L3000 is 50 min, accompanied by the solution color change from blue to yellow, whereas the formation of polymer-d3000 needs longer time up to 72 h (Fig. 5a, b and Supplementary Figs. 55–57). The mechanism of the chiral recognition is that the presence of different competitiveness units exerts a strong and different retardation during the assembly kinetics, where –NH2, –OH, and –COOH on l- and d-Arg have different stereo conformation and binding energy (Supplementary Fig. 58). The theoretical calculation shows that the binding energy between metastable LSM3000 with SG7 (−52.64 kJ mol−1) is weaker than that of LSM3000 with l-Arg (−191.50 kJ mol−1) and LSM3000 with d-Arg (−471.64 kJ mol−1), providing basic conditions for co-assembly (Supplementary Figs. 46 and 58). Further, the difference in the binding energy between l-Arg and d-Arg gives LSM3000 the ability to recognize the Arg type (Fig. 5c, d)62. To verify the reproducibility of chiral recognition, three batches of metastable LSM3000 samples were prepared, and the results showed that the lag time for the formation of corresponding polymer-L3000 was 50 min (Supplementary Fig. 59). Importantly, the chiral recognition effectiveness from metastable LSM20, LSM50, and LSM100 and LSM3000 is quite distinct (Supplementary Figs. 60–62 and the detailed analysis). For the smaller metastable LSM20 and LSM50 with higher activity, chiral recognition is difficult to realize due to indistinguishable lag time. For metastable LSM100, the identification can only be seen within 20 min. Thus, the higher recognition ability of metastable LSM3000 maybe origin in relatively maximized kinetics effects and minimal activity.
Universality
As proof of universality of our proposed preparation method for LSP, various simple molecules with aromatic rings and negative charge (Fig. 6) were chosen to orderly array in confinement space of LDH (Supplementary Figs. 63–77) and further performed the same assembly process as SG7 (Supplementary Figs. 78–83). By the assembly results, the basic principles are summarized as follows: (1) the formation of H-bond net among –NH2,−HSO3, and −OH. For example, the benzenesulfonic acid (BSA) with insufficient active H-bond cannot form H-bond net (Supplementary Figs. 64, 67, and 70), leading to the failure in formation of LSP (Supplementary Fig. 79). In contrast, the appearance of multiple active H-bonds easily forms the LSPs (e.g., 3-aminobenzene sulfonic acid; 2,5-diaminobenzenesulfonic acid; Congo red, CR); (2) oriented long-range π-π stacking between neighboring molecules. For example, the 6,7-dihydroxynaphthalene-2-sulfonate (DHNS) shows the failed formation of LSP (Supplementary Fig. 82). The reasons originate from that although the appearance of π-π stacking of DHNS molecules in the interlayer of LDH as confirmed by UV spectrum (Supplementary Fig. 65), the π-π stacking is the negligible long-range ordered arrangement as confirmed by a low FL anisotropic value (r = 0.0214) (Supplementary Fig. 68). AIMD simulations, which are referred to XRD, ICP-MS, and elemental analysis results (Supplementary Fig. 63, Table 7), also further proved the adjacent DHNS molecules in the interlayer of LDH do have π-π stacking in short range, attributing to three functional groups and the large aromatic ring of DHNS. However, they show a disordered arrangement in the long range (Supplementary Fig. 73). Therefore, our approach provides a good alternative to form oversized LSP by simple molecules.
In conclusion, we propose a universal method for establishing LSP with simple molecules. The molecules equipped with functional groups to access cooperative intermolecular interactions (π-π stacking and H-bond, etc.) are monomer candidates for LSP. In our proposed protocol, the simple molecules in LDH confinement undergo newly orientated long-range π-π stacking and was kept in the energy well, which overcomes a huge energy barrier to inhibit spontaneous aggregation of monomer and depolymerization of metastable state. The living assembly and seed-induced growth provide activity and length control, as well as narrow dispersity of LSP. Owing to the competitive growth at anisotropic active sites, the largest DP reported so far (~6000) has been obtained. The distinct living abilities from LSP20−LSP3000 provide the possibility of control according to actual requirements. We affirm that our system definitely is not a special case. A similar LSP can be established by other molecules, which can also induce the transformation from inactivated aggregates to ordered rearrangements. The living character endows LSP with explicit temporal control in complex dynamic programming, which should be a significant step toward the further development of chiral amplification, copolymer formation, and artificial biomaterials. It is anticipated that this strategy will broaden the spread of monomer candidates, leading to more possibilities for LSP in various domains, such as autonomous materials and regenerative medicine.
Data availability
All data supporting the findings are available in the article as well as the supplementary information files and from the authors on reasonable request.
References
Aida, T., Meijer, E. W. & Stupp, S. I. Functional supramolecular polymers. Science 335, 813–817 (2012).
Goor, O. J. G. M., Hendrikse, S. I. S., Dankers, P. Y. W. & Meijer, E. W. From supramolecular polymers to multi-component biomaterials. Chem. Soc. Rev. 46, 6621–6637 (2017).
Wang, W. et al. Recent advances in thermoplastic elastomers from living polymerizations: macromolecular architectures and supramolecular chemistry. Prog. Polym. Sci. 95, 1–31 (2019).
Dou, X. et al. Supramolecular hydrogels with tunable chirality for promising biomedical applications. Acc. Chem. Res. 53, 852–862 (2020).
Krieg, E., Weissman, H., Shirman, E., Shimoni, E. & Rybtchinski, B. A recyclable supramolecular membrane for size-selective separation of nanoparticles. Nat. Nanotechnol. 6, 141–146 (2011).
Yu, L., Wang, Z., Wu, J., Tu, S. & Ding, K. Directed orthogonal self-assembly of homochiral coordination polymers for heterogeneous enantioselective hydrogenation. Angew. Chem. Int. Ed. 49, 3627–3630 (2010).
Würthner, F. Supramolecular polymerization: living it up. Nat. Chem. 6, 171–173 (2014).
Wang, H. et al. Living supramolecular polymerization of an aza-BODIPY dye controlled by a hydrogen-bond-accepting triazole unit introduced by click chemistry. Angew. Chem. Int. Ed. 59, 5185–5192 (2020).
Ghosh, G., Dey, P. & Ghosh, S. Controlled supramolecular polymerization of π-systems. Chem. Commun. 56, 6757–6769 (2020).
Wehner, M. & Würthner, F. Supramolecular polymerization through kinetic pathway control and living chain growth. Nat. Rev. Chem. 4, 38–53 (2020).
Sorrenti, A., Leira-Iglesias, J., Markvoort, A. J., de Greef, T. F. A. & Hermans, T. M. Non-equilibrium supramolecular polymerization. Chem. Soc. Rev. 46, 5476–5490 (2017).
Wagner, W., Wehner, M., Stepanenko, V., Ogi, S. & Würthner, F. Living supramolecular polymerization of a perylene bisimide dye into fluorescent J-aggregates. Angew. Chem. Int. Ed. 56, 16008–16012 (2017).
Gao, L., Lin, J., Zhang, L. & Wang, L. Living supramolecular polymerization of rod–coil block copolymers: kinetics, origin of uniformity, and its implication. Nano Lett. 19, 2032–2036 (2019).
Qin, B. et al. Supramolecular interfacial polymerization: a controllable method of fabricating supramolecular polymeric materials. Angew. Chem. Int. Ed. 56, 7639–7643 (2017).
Ogi, S., Sugiyasu, K., Manna, S., Samitsu, S. & Takeuchi, M. Living supramolecular polymerization realized through a biomimetic approach. Nat. Chem. 6, 188–195 (2014).
Ogi, S., Stepanenko, V., Sugiyasu, K., Takeuchi, M. & Würthner, F. Mechanism of self-assembly process and seeded supramolecular polymerization of perylene bisimide organogelator. J. Am. Chem. Soc. 137, 3300–3307 (2015).
Zhang, W., Jin, W., Fukushima, T., Mori, T. & Aida, T. Helix sense-selective supramolecular polymerization seeded by a one-handed helical polymeric assembly. J. Am. Chem. Soc. 137, 13792–13795 (2015).
Wagner, W., Wehner, M., Stepanenko, V. & Würthner, F. Impact of molecular shape on supramolecular copolymer synthesis in seeded living polymerization of perylene bisimides. CCS Chem. 1, 598–613 (2019).
Mukhopadhyay, R. D. & Ajayaghosh, A. Living supramolecular polymerization. Science 349, 241–242 (2015).
Kang, J. et al. A rational strategy for the realization of chain-growth supramolecular polymerization. Science 347, 646–651 (2015).
Zhang, H. et al. Programming chain-growth copolymerization of DNA hairpin tiles for in-vitro hierarchical supramolecular organization. Nat. Commun. 10, 1006 (2019).
McMillan, J. R., Hayes, O. G., Remis, J. P. & Mirkin, C. A. Programming protein polymerization with DNA. J. Am. Chem. Soc. 140, 15950–15956 (2018).
Robinson, M. E. et al. Length control of supramolecular polymeric nanofibers based on stacked planar platinum(II) complexes by seeded-growth. Chem. Comm. 51, 15921–15924 (2015).
Lutz, J.-F., Lehn, J.-M., Meijer, E. W. & Matyjaszewski, K. From precision polymers to complex materials and systems. Nat. Rev. Mater. 1, 16024–16038 (2016).
Dhiman, S., Jain, A., Kumar, M. & George, S. J. Adenosine-phosphate-fueled, temporally programmed supramolecular polymers with multiple transient sates. J. Am. Chem. Soc. 139, 16568–16575 (2017).
Mishra, A. et al. Biomimetic temporal self-assembly via fuel-driven controlled supramolecular polymerization. Nat. Commun. 9, 1295 (2018).
Qin, B. et al. Supramolecular polymer chemistry: from structural control to functional assembly. Prog. Polym. Sci. 100, 101167 (2020).
Fukui, T., Sasaki, N., Takeuchi, M. & Sugiyasu, K. Living supramolecular polymerization based on reversible deactivation of a monomer by using a ‘dummy’ monomer. Chem. Sci. 10, 6770–6776 (2019).
Zhang, K., Yeung, M. C., Leung, S. Y. & Yam, V. W. Living supramolecular polymerization achieved by collaborative assembly of platinum(II) complexes and block copolymers. PNAS 114, 11844–11849 (2017).
Adamala, K. & Szostak, J. W. Competition between model protocells driven by an encapsulated catalyst. Nat. Chem. 5, 495–501 (2013).
Grochmal, A., Prout, L., Makin-Taylor, R., Prohens, R. & Tomas, S. Modulation of reactivity in the cavity of liposomes promotes the formation of peptide bonds. J. Am. Chem. Soc. 137, 12269–12275 (2015).
Segré, D., Ben-Eli, D., Deamer, D. W. & Lancet, D. The lipid world. Orig. Life Evol. Biosph. 31, 119–145 (2001).
Walde, P. Building artificial cells and protocell models: experimental approaches with lipid vesicles. BioEssays 4, 296–303 (2010).
Murillo-Sanchez, S., Beaufils, D., Mañas, J. M. G., Pascal, R. & Ruiz-Mirazo, K. Fatty acids’ double role in the prebiotic formation of a hydrophobic dipeptide. Chem. Sci. 7, 3406–3413 (2016).
Mansy, S. S. et al. Template-directed synthesis of a genetic polymer in a model protocell. Nature 454, 122–125 (2008).
Adamala, K. & Szostak, J. W. Nonenzymatic template-directed RNA synthesis inside model protocells. Science 342, 1098–1100 (2013).
Adamala, K. & Szostak, J. W. Collaboration between primitive cell membranes and soluble catalysts. Nat. Commun. 7, 11041 (2016).
van den Berg, B., Wain, R., Dobson, C. M. & Ellis, R. J. Macromolecular crowding perturbs protein refolding kinetics: implications for folding inside the cell. EMBO J. 19, 3870–3875 (2000).
Chakraborty, K. et al. Chaperonin-catalyzed rescue of kinetically trapped states in protein folding. Cell 142, 112–122 (2010).
Hartl, F. U. & Hayer-Hartl, M. Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 295, 1852–1858 (2002).
Langton, M. J., Keymeulen, F., Ciaccia, M., Williams, N. H. & Hunter, C. A. Controlled membrane translocation provides a mechanism for signal transduction and amplification. Nat. Chem. 9, 426–430 (2017).
Taviot-Guého, C. et al. Tailoring hybrid layered double hydroxides for the development of innovative applications. Adv. Funct. Mater. 28, 1703868 (2018).
Shi, W., Wei, M., Lu, J., Evans, D. G. & Duan, X. Studies on the orientation and polarized photoluminescence of α-naphthalene acetate in the layered double hydroxide matrix. J. Phys. Chem. C. 113, 12888–12896 (2009).
Fu, H.-R., Wu, X.-X., Ma, L.-F., Wang, F. & Zhang, J. Dual-Emission SG7@MOF sensor via SC−SC transformation: enhancing the formation of excimer emission and the range and sensitivity of detection. ACS Appl. Mater. Interfaces 10, 18012–18020 (2018).
Constantino, V. R. L. & Pinnavaia, T. J. Structure-reactivity relationships for basic catalysts derived from a Mg2+/A13+/CO3− layered double hydroxide. Catal. Lett. 23, 361–368 (1994).
Williams, G. R., Fogg, A. M., Sloan, J., Taviot-Guého C. & O’Hare, D. Staging during anion-exchange intercalation into [LiAl2(OH)6] Cl.yH2O: structural and mechanistic insights. Dalton Trans. 32, 3499−3506 (2007).
Zhao, J., Liu, Y., Hao, A. & Xing, P. High-throughput synthesis of chiroptical nanostructures from synergistic hydrogen-bonded coassemblies. ACS Nano 14, 2522–2532 (2020).
Zhang, K., Yeung, M. C.-L., Leung, S. Y.-L. & Yam, V. W.-W. Energy landscape in supramolecular coassembly of platinum(II) complexes and polymers: morphological diversity, transformation, and dilution stability of nanostructures. J. Am. Chem. Soc. 140, 9594–9605 (2018).
Guo, D. et al. Programmed coassembly of one-dimensional binary superstructures by liquid soft confinement. J. Am. Chem. Soc. 140, 18–21 (2018).
Jiang, B. et al. Tunable-sized polymeric micelles and their assembly for the preparation of large mesoporous platinum nanoparticles. Angew. Chem. Int. Ed. 55, 10037–10041 (2016).
Niu, F. et al. Disassembly of supported Co and Ni nanoparticles by carbon deposition for the synthesis of highly dispersed and active catalysts. ACS Catal. 10, 6231–6239 (2020).
Liu, Y. et al. In-plane highly dispersed Cu2O nanoparticles for seeded lithium deposition. Nano Lett. 19, 4601–4607 (2019).
Debye, P. Molecular-weight determination by light scattering. J. Phy. Colloid Chem. 51, 18–32 (1947).
Ogi, S., Grzeszkiewicz, C. & Würthner, F. Pathway complexity in the self-assembly of a zinc chlorin model system of natural bacteriochlorophyll J-aggregates. Chem. Sci. 9, 2768–2773 (2018).
Wagner, W., Wehner, M., Stepanenko, V. & Würthner, F. Supramolecular block copolymers by seeded living polymerization of perylene bisimides. J. Am. Chem. Soc. 141, 12044–12054 (2019).
Valera, J. S., Gomez, R. & Sánchez, L. Kinetic traps to activate stereomutation in supramolecular polymers. Angew. Chem. Int. Ed. 58, 510–514 (2019).
Fukui, T. et al. Control over differentiation of a metastable supramolecular assembly in one and two dimensions. Nat. Chem. 9, 493–499 (2017).
Colomb-Delsuc, M., Mattia, E., Sadownik, J. W. & Otto, S. Exponential self-replication enabled through a fibre elongation/breakage mechanism. Nat. Commun. 6, 7427 (2015).
Valera, J. S., Gómez, R. & Sánchez, L. Tunable energy landscapes to control pathway complexity in self-assembled N-heterotriangulenes: living and seeded supramolecular polymerization. Small 14, 1702437 (2018).
Helmich, F., Smulders, M. M. J., Lee, C. C., Schenning, A. P. H. J. & Meijer, E. W. Effect of stereogenic centers on the self-sorting, depolymerization, and atropisomerization kinetics of porphyrin-based aggregates. J. Am. Chem. Soc. 133, 12238–12246 (2011).
Sarkar, S., Sarkar, A. & George, S. J. Stereoselective seed-induced living supramolecular polymerization. Angew. Chem. Int. Ed. 59, 19841–19845 (2020).
Ma, X. et al. Fabrication of chiral-selective nanotubular heterojunctions through living supramolecular polymerization. Angew. Chem. Int. Ed. 55, 9539–9543 (2016).
Acknowledgements
This work was supported by the National Key Research and Development Program (2019YFC1906100), National Natural Science Foundation of China (21974008, 21571014, 21656001, and 21521005), and the Fundamental Research Funds for the Central Universities (12060093063).
Author information
Authors and Affiliations
Contributions
W.S. and C.L. conceived and directed the project. Y.Z. carried out the experimental work. S.X. carried out the theoretical calculation. All authors co-wrote the manuscript. Y.Z. and S.X. contributed equally to this work.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Peer review information Nature Communications thanks Daigo Miyajima and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Zong, Y., Xu, SM., Shi, W. et al. Oriented arrangement of simple monomers enabled by confinement: towards living supramolecular polymerization. Nat Commun 12, 2596 (2021). https://doi.org/10.1038/s41467-021-22827-4
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41467-021-22827-4
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.