Abstract
MicroRNAs (miRNAs) may function as either oncogenes or tumor suppressors in the malignant progression of different tumor types. MiR-663 was recently reported to be decreased and identified as a tumor suppressor in gastric cancer. We also verified its role in repressing cell proliferation of a gastric cancer cell line. In this study, however, miR-663 was found to be upregulated in nasopharyngeal carcinoma (NPC) cells compared with human immortalized nasopharyngeal epithelium cells, using a miRNA microarray, and this higher expression was confirmed in NPC tissue samples. Indeed, inhibition of miR-663 impaired the proliferation of NPC cells in vitro and the NPC tumor growth of xenografts in nude mice. Mechanistically, miR-663 directly targeted p21WAF1/CIP1 to promote the cellular G1/S transition, as the inhibitory effects of miR-663 on the G1/S transition could be rescued by p21WAF1/CIP1 silencing. Our results imply that miR-663 may act as an oncogene in NPC. The newly identified miR-663/p21WAF1/CIP1 axis clarifies the molecular mechanism of NPC cell proliferation and represents a novel strategy for the diagnosis and treatment of patients with NPC.
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Acknowledgements
We thank Jun Li for kindly providing the human gastric cancer cells MKN-45 and SCG-7901. This work was supported by grants from the National Natural Science Foundation of China (30973506 and 81172345), the 863 Project (2006AA02A404), the Science Foundation of Key Hospital Clinical Program of Ministry of Health, China (2010-178), and the Project of State Key Laboratory of Oncology in South China.
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Yi, C., Wang, Q., Wang, L. et al. MiR-663, a microRNA targeting p21WAF1/CIP1, promotes the proliferation and tumorigenesis of nasopharyngeal carcinoma. Oncogene 31, 4421–4433 (2012). https://doi.org/10.1038/onc.2011.629
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DOI: https://doi.org/10.1038/onc.2011.629
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