Researchers at the University of Washington have developed a new high-throughput method to map chromatin interactions genome-wide. Named DNase Hi-C, this methodology uses DNase I for chromatin fragmentation, thus overcoming limitations on efficiency and resolution imposed on previous methods such as conventional Hi-C, which uses restriction enzymes for fragmentation. Among other experiments, the team validated their approach in two human cell lines by coupling DNase Hi-C with a targeted DNA sequence capture technology to map fine-scale three-dimensional chromatin architecture of 998 promoters of genes encoding long intergenic non-coding RNAs. Expression of lincRNAs was found to be tightly controlled via super-enhancers and Polycomb repressive complex 2.