Abstract
Disruption of the peroxisomal acyl-CoA oxidase 1 (Acox1) gene in the mouse results in the development of severe microvesicular hepatic steatosis and sustained activation of peroxisome proliferator-activated receptor-α (PPARα). These mice manifest spontaneous massive peroxisome proliferation in regenerating hepatocytes and eventually develop hepatocellular carcinomas. Human ACOX1, the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, has two isoforms including ACOX1a and ACOX1b, transcribed from a single gene. As ACOX1a shows reduced activity toward palmitoyl-CoA as compared with ACOX1b, we used adenovirally driven ACOX1a and ACOX1b to investigate their efficacy in the reversal of hepatic phenotype in Acox1(−/−) mice. In this study, we show that human ACOX1b is markedly effective in reversing the ACOX1 null phenotype in the mouse. In addition, expression of human ACOX1b was found to restore the production of nervonic (24:1) acid and had a negative impact on the recruitment of coactivators to the PPARα-response unit, which suggests that nervonic acid might well be an endogenous PPARα antagonist, with nervonoyl-CoA probably being the active form of nervonic acid. In contrast, restoration of docosahexaenoic (22:6) acid level, a retinoid-X-receptor (RXRα) agonist, was dependent on the concomitant hepatic expression of both ACOX1a and ACOX1b isoforms. This is accompanied by a specific recruitment of RXRα and coactivators to the PPARα-response unit. The human ACOX1b isoform is more effective than the ACOX1a isoform in reversing the Acox1 null phenotype in the mouse. Substrate utilization differences between the two ACOX1 isoforms may explain the reason why ACOX1b is more effective in metabolizing PPARα ligands.
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Main
Mice lacking acyl-CoA oxidase 1 (Acox1), which catalyzes the first and rate-limiting enzyme of the peroxisomal fatty acid β-oxidation pathway of very-long-chain fatty acids (VLCFAs), develop severe microvesicular steatohepatitis with increased intrahepatic H2O2 levels and hepatocellular regeneration.1, 2 Liver cell proliferation leads to complete replacement of steatotic hepatocytes with hepatocytes that exhibit massive spontaneous peroxisome proliferation.1 Older Acox1(−/−) mice develop hepatocellular carcinomas due to the sustained activation of peroxisome proliferator-activated receptor-α (PPARα).2 Mice nullizygous for both Pparα and Acox1 (Pparα(−/−), Acox1(−/−)), have shown a lack of spontaneous hepatic peroxisome proliferation and a marked decrease in hepatic steatosis, which emphasizes the critical role of both PPARα and ACOX1 in hepatic metabolism and in the pathogenesis of specific fatty liver phenotype.3 Sustained activation of PPARα and transcriptional activation of PPARα target genes in the liver of mice lacking ACOX1 highlight the role of peroxisomal ACOX1 in metabolizing the endogenous natural PPARα ligands.4
At the transcriptional level, PPARα, which forms a heterodimer with retinoid X receptor (RXR),5, 6 binds to peroxisome proliferator-responsive element (PPRE) present in the 5′-flanking region of target genes.6, 7, 8 In the presence of a ligand, PPAR–RXR complexes recruit coactivators, such as steroid receptor coactivator-1 (SRC-1) and PPAR-binding protein/Mediator 1 (PBP/TRAP220/Med1)9, 10 to enhance target gene transcription.9, 10, 11 The generation of Pparα−/− mice established that PPARα is essential for hepatic peroxisome proliferation and coordinates transcriptional activation of genes coding for ACOX1, L-peroxisomal bifunctional enzyme (L-PBE), peroxisomal thiolase (PTL), cytochrome P4504A10 (CYP4A10) and cytochrome P4504A14 (CYP4A14), and other lipid metabolism-related key enzymes by structurally diverse synthetic peroxisome proliferators, as well as by several fatty acids and their polyunsaturated derivatives.12, 13, 14, 15
Although fatty acids are metabolized by the mitochondrial and peroxisomal β-oxidation enzyme systems with partly overlapping substrate specificities,3, 16 the oxidation of the major fraction of medium and long-chain fatty acids (LCFAs, C12-C18) occurs in the mitochondria, whereas oxidation of VLCFAs takes place almost exclusively in peroxisomes.3, 16 Both LCFAs and VLCFAs (>C18) are also metabolized by the microsomal CYP4A10 and CYP4A14 fatty acid ω-hydroxylases, resulting in the formation of dicarboxylic acids (DCAs) that are further degraded by the peroxisomal β-oxidation system.16, 17
In several peroxisomal disorders, the peroxisomal fatty acid β-oxidation pathway is defective either with a decreased number or the absence of morphologically distinguishable peroxisomes in the liver. One of these peroxisomal disorders is pseudoneonatal adrenoleukodystrophy (P-NALD), caused by ACOX1 deficiency.18 The ACOX1 enzyme is encoded by a single gene, which generates two splice variants, including exon 3a or exon 3b, respectively,19, 20 leading to the synthesis of two protein isoforms ACOX1a or ACOX1b.21 A single homozygous mutation in exon 3b results in the development of the P-NALD disease,22 suggesting different substrate specificities of the two ACOX1 isoforms. On the other hand, further biochemical analysis of recombinant human ACOX1 isoforms showed that ACOX1a seems to be more labile and exhibits only 50% specific activity toward palmitoyl-CoA as compared with ACOX1b.21
Therefore, in an attempt to approach the in vivo conditions and to understand the sensing metabolic role of ACOX1 vis-à-vis the hepatic steatosis and the activation of PPARα, we expressed human ACOX1 isoforms in Acox1(−/−) null mice by adenofection. We then analyzed the impact of human ACOX1 expression on the pathophysiological aspects and on the regulation of PPARα and its target genes in the liver samples obtained from Acox1−/− mice. We now show that the hepatic expression of human ACOX1b isoform reverses the phenotype of Acox1(−/−) null mice.
MATERIALS AND METHODS
Adenoviral Gene Transfer
Construction of recombinant adenovirus containing the human ACOX1a or ACOX1b cDNA (Ad/hACOX1a or Ad/hACOX1b) was as follows. Human ACOX1a or ACOX1b cDNAs21 were cloned into the BglII and HindIII sites of pShuttle-CMV expression vector (Quantum Biotechnologies). A nucleotide sequence encoding 6xHis-tag was introduced into the 5′-flanking regions of each cDNA between BglII/KpnI sites. The linearized pShuttle vector and AdEasy vector (Quantum Biotechnologies) were then cotransformed into the Escherichia coli strain BJ5183. Positive recombinant plasmid Ad/hACOX1a or Ad/hACOX1b were selected. The Ad/hACOX1a or Ad/hACOX1b virus was then generated as described previously.23 The adenoviral construct of Ad/LacZ was a generous gift from Dr W El-Deiry (University of Pennsylvania, Philadelphia, PA, USA) and has been described previously.23
Mice and Treatments
Both wild-type (C57BL/6J) and Acox1−/− mice,1, 2 6–10 weeks of age, were used in this study. Mice were kept under a 12-h light/12-h dark cycle, and maintained on powdered chow and water ad libitum. Recombinant adenovirus Ad/hACOX1a, Ad/hACOX1b or Ad/lacz were injected intravenously through the tail vein, in a volume of 200 μl with 2 × 1011 virus particles and killed 5 days after injection. For cell proliferation analysis, mice were administered bromodeoxyuridine (BrdUrd) (1 mg/ml) in drinking water for 3 days after adenovirus injection, and their livers were processed for immunohistochemistry.23 The animal procedures used in this study were approved by the Institutional Review Boards for Animal Research of the Northwestern University.
RESULTS
ACOX1: One Gene, Two Splice Variants with Differential Tissue Expressions
In both humans and mouse, the ACOX1 gene consists of 15 exons and 14 introns, with exon 3 presenting as two variants 3a and 3b, respectively, leading to the synthesis of an ACOX1a and ACOX1b isoform.19, 20, 21 Indeed, alternative splicing of pre-mRNA produces two mRNA sequences harboring exon 3a or exon 3b, which both code for a 54 amino-acid region located near the N terminus. This region is highly conserved in several species with high amino-acid identity.19, 20 Alignment of amino-acid sequences from humans (hEx3a and hEx3b) and mouse (mEx3a and mEx3b) shows 87% identity between hACOX1a and mACOX1a, and 94% identity between hACOX1b and mACOX1b (Supplementary Figure S1A). This strong conservation through evolution suggests that the two isoforms may well have different physiological functions. In human tissues, both the a and the b isoforms show essentially similar levels of expression (Supplementary Figure S1B). In the mouse, the Acox1b mRNA level appeared higher in white adipose tissue (Supplementary Figure S1B). Higher level of expression of 1a over 1b in the liver and kidney or inversely 1b over 1a in the brain, lung, muscle, white adipose tissue and testis are evident. In the human heart, both splice variants are expressed equally (Supplementary Figure S1B). This differential expression shows the existence of a tissue-dependent splice regulation of Acox1 mRNAs.
General Approach
The availability of Acox1(−/−) mice enabled us to determine the immediate effects of transient expression of human ACOX1 isoforms a and b21 on PPARα-mediated pleiotropic responses in mouse hepatocytes in vivo. Acox1(−/−) mice develop severe microvesicular steatohepatitis, which leads to progressive hepatocellular regeneration. The regenerated hepatocytes exhibit massive spontaneous peroxisome proliferation accompanied by a sustained activation of PPARα and its target genes.1, 2, 3 The expression of human ACOX1 isoforms by adenofection in the Acox1(−/−) mouse liver was attested by real-time quantitative PCR at the mRNA level and by western blotting and measurement of enzymatic activity toward palmitoyl-CoA (Table 1 and Supplementary Figure S2). Results obtained from time-course experiments (3, 5 and 7 days) show that the expression level of ACOX1a or ACOX1b reaches a maximum at 5 days after infection and declines abruptly at 7 days (Supplementary Figures S3 and S4). Despite a higher adenofection dose, it was not possible to express ACOX1a to a similar extent as ACOX1b in Acox1(−/−) mouse liver (Supplementary Figure S5). However, additional analyses using real-time PCR and immunostaining methods show an evident expression of ACOX1a at both mRNA and protein levels. However, when compared with the activity level of ACOX1b, ACOX1a expression was 10-fold lower (Table 1 and Supplementary Figures S3–S5).
Influence of Human ACOX1 Isoform Expression on Hepatic Steatosis in ACOX1 Null Mouse
The absence of ACOX1 in the liver of ACOX1 null mice caused microvesicular fatty metamorphosis of hepatocytes (Figure 1a). Fatty changes were detected in all hepatocytes irrespective of their distribution within the liver lobule, but were not found in regenerated liver cells after their emergence. These showed abundant intensely eosinophilic granular cytoplasm (Figure 1a). The expression of the ACOX1a isoform in the Acox1−/− mouse liver (Figure 1a) resulted in a modest reduction in hepatic steatosis as compared with Acox1(−/−) livers. On the other hand, the expression of ACOX1b resulted in a significant reduction in hepatic steatosis (Figure 1a and −/−1b insert). The expression of ACOX1b was robust in many hepatocytes as concluded from the intense cytoplasmic peroxidase staining, whereas in other hepatocytes, the expression was less pronounced (Figure 1b and −/−1b insert). The concomitant expressions of both ACOX1a and ACOX1b isoforms show a near complete reversal of fatty liver phenotype in Acox1−/− (Figure 1b and −/−1b insert). In addition, when the expression level of ACOX1a isoform is enhanced twice in the Acox1(−/−) liver, we observed only a week reversal of the hepatic steatosis phenotype in these mice (Supplementary Figure S5).
Hepatic Lipid Storage and Serum Fatty Acid Profiles
Human ACOX1a expression in the Acox1 null mouse liver resulted in a mild reduction in neutral lipid content and this correlated with an appreciable reduction in microvesicular steatosis in hepatocytes (Figure 2). The expression of ACOX1b resulted in a marked decrease in the neutral lipid oil-red O staining when compared with Acox1(−/−) null mice (Figure 2, Wt and −/−1b inserts). The expression of both isoforms 1a and 1b in the Acox1(−/−) liver essentially reversed hepatic steatosis with a staining lipid profile resembling the wild-type phenotype (Figure 2, Wt and −/−1ab inserts).
In ACOX1 null mice, the absence of peroxisomal fatty acid oxidation resulted in a decrease in circulating triacylglycerol (TG) level (Supplementary Figure S3C). The expression of each ACOX1 isoform shows a significant increase in serum TG at 3 days for ACOX1a and at both 3 and 5 days for ACOX1b (Supplementary Figure S3 and S4). The expression of both human isoforms 1a and 1b in Acox1(−/−) mice is accompanied by a strong hypertriglyceridemia (Supplementary Figure S5). Plasma cholesterol level shows a significant increase only when both ACOX1 isoforms are coexpressed in the Acox1−/− liver (Supplementary Figure S5).
ACOX1B Controls the Spontaneous Hepatic Peroxisome Proliferation
Visualization of semithin sections of the liver of 2-month-old Acox1(−/−) mice, showed a paucity of peroxisomes in the majority of hepatocytes that exhibit microvesicular lipid droplets. In these cells, the diaminobenzidine-positive catalase-containing organelles were sparse (Figure 3 and −/− lower left insert). As reported previously, Acox1(−/−) livers showed progressive cell proliferation, and the newly emerging hepatocytes show spontaneous peroxisome proliferation (Figure 3 and −/− lower right insert). Acox1(−/−) livers, in which the ACOX1a isoform was expressed, exhibited many fatty hepatocytes but with smaller lipid droplets (Figure 3 and −/−1a upper right insert) when compared with the larger fat droplets in the Acox1(−/−) liver (Figure 3 and −/− left lower insert). In the liver of the Acox1 null mice, expressing the ACOX1b isoform, the majority of hepatocytes displayed a remarkable peroxisome proliferation with rare fatty hepatocytes with microvesicular lipid droplets (Figure 3 and −/−1b insert). Expression of both the ACOX1a and the ACOX1b isoforms in the mouse Acox1(−/−) liver was associated with an intermediary profile including many fatty hepatocytes with a strongly reduced number of lipid vesicles and some regenerative hepatocytes with numerous peroxisomes (Figure 3 and −/−1ab insert).
Liver Cell Proliferation in ACOX1(−/−) Null Mice
A strong nuclear BrdUrd staining of hepatocytes was found in Acox1(−/−) livers due to increased hepatocellular regeneration (Figure 4a, upper right, and b). Five days after injection of Ad/ACOX1a or Ad/ACOX1b into ACOX1 null mice, we noted a significant reduction in the number of labeled hepatocyte nuclei (Figure 4a, −/−1a, −/−1b, and b). On the other hand, expression of both the ACOX1a and the ACOX1b isoforms led to an increase in stained nuclei of both hepatocytes and hepatic nonparenchymal cells (Figure 4a, −/−1ab insert, and b).
ACOX1-Dependent Regulation of PPARα Target Genes
Real-time PCR and northern blot analysis of liver RNA showed spontaneous upregulation of PPARα target gene expression in the Acox1(−/−) liver (Figure 5a and b). As expected, mRNA levels of known PPARα-regulated genes responsible for peroxisomal (L-PBE, PTL), microsomal (CYP4A10, CYP4A14) or mitochondrial (CPT1a and MCAD) fatty acid oxidation were elevated in the Acox1−/− liver due to the spontaneous activation of PPARα by unmetabolized endogenous ligands (Figure 5a and b). Adenofection with the ACOX1a isoform resulted in a modest reduction in the levels of these mRNAs, whereas ACOX1b had a more profound effect in reversing the increases. Administration of both isoforms together seemed to be more effective. Fatty acid transporter/CD36 mRNA content was also elevated in the Acox1(−/−) liver and the adenofected ACOX1 isoforms reversed this effect (Figure 5a). The amount of the three PPAR isotypes, α, β/δ and γ, was increased in the Acox1(−/−) null mouse liver (Figure 5a). Expression of the ACOX1a and ACOX1b isoforms in the livers obtained from the Acox1(−/−) mouse showed a significant decrease in mRNA levels of the PPARα and its target genes, as well as in mRNA levels of PPARβ/δ and PPARγ (Figure 5a).
Immunoblot analysis confirmed an increase in the content of L-PBE and PTL in the Acox1(−/−) mouse liver (Figure 5c). Both the expression of ACOX1b alone or ACOX1a plus ACOX1b, showed a quasi-normal expression of both peroxisomal L-PBE and PTL proteins. To a lesser extent, the expression of ACOX1a alone also had a significant effect (Figure 5c). The immunoblot results have been confirmed by the ectopic adenofection of isolated Acox1−/− hepatocytes as shown in Figure 5c.
Recruitment of PPARα, RXRα and Coactivators SRC-1 and PBP to L-PBE Promoter
Transcriptional activation of a target gene by PPARα requires, in addition to heterodimerization with RXRα, recruitment of coactivators. ChIP analysis was used to examine whether recruitments of PPARα, RXRα and coactivators (SRC-1 and PBP/Med1) to the L-PBE gene promoter were influenced by the hepatic expression of the ACOX1 isoforms in Acox1 null mice.
Livers from Acox1(−/−) mice, as control, or Acox1−/−mice expressing LacZ/adenovirus, ACOX1a, ACOX1b or both isoforms 1a and 1b of ACOX1 were used to prepare cross-linked protein-DNA extracts. The latter were immunoprecipitated with antibodies against PPARα, RXRα, PBP/Med1 and SRC-1 (Figure 6a). DNA fragments extracted from these immunoprecipitates were analyzed by PCR using primers to amplify the PPRE of the mouse L-PBE gene. These results show that infection with mock LacZ/adenovirus has no effect on the PPARα/RXRα-PBP/Med1-SRC1 complex recruitment (Figure 6a, lines 1 and 2). Hepatic expression of ACOX1a or ACOX1b exhibited a similar level of PPARα/RXRα recruitment (Figure 6a, lines 3 and 4). However, in the case of ACOX1b expression, there was a net downrecruitment of SRC1 and PBP/Med1 to the L-PBE promoter (Figure 6a, line 4). This is in accordance with the downregulated level of L-PBE mRNA (Figure 5a and b). The expression of both ACOX1 isoforms 1a and 1b showed a unique profile of the L-PBE PPAR-response element in which the recruitments of PBP/Med1 and of SRC-1 were accompanied by an increased recruitment of RXRα and a net decrease in PPARα recruitment (Figure 6a, line 5).
Thus, the expression of each ACOX1 isoform or both together resulted in the recruitment of a specific transcriptional complex, including PPARα and/or RXRα and coactivators, suggesting that fatty acids metabolized by ACOX1 are key ligands for PPARα or for RXRα under in vivo conditions.
Serum Levels of Fatty Acids
The decreased fatty acid levels of ω-3 (−79%) and ω-6 (−36%) series as measured in Acox1(−/−) mice were reversed significantly by the expression of ACOX1b alone (−54% for ω-3 and −11% for ω-6) or in association with ACOX1a (−61% for ω-3 and −25% for ω-6) (Supplementary Figure S6A). Polyunsaturated fatty acids, such as arachidonic acid (AA, 20:4ω-6) and docosahexaenoic acid (DHA, 22:6ω-3), are known substrates (or products) of peroxisomal fatty acids oxidation16 and function as natural ligands for PPARα.12, 13, 14, 15 Plasma levels of these polyunsaturated fatty acids appear to be linked to the expression level of hepatic ACOX1 isoforms. Indeed, in the absence of Acox1 a remarkable decrease in the serum levels of AA (20:4) and DHA (22:6) was found (Figure 6b and c). The expression of ACOX1a isoform alone had no effect on the levels of 20:4 and 22:6, whereas ACOX1b isoform significantly increase the level of 22:6 (Figure 6b and c). Interestingly, the expression of both ACOX1 isoforms exhibited significant increases in 20:4 and 22:6 levels (Figure 6b and c). Among different VLCFAs, the monounsaturated nervonic acid (24:1) level increased strongly upon ACOX1b expression (Figure 6d). In addition, plasma levels of other saturated and monunsaturated fatty acids from 16:0 to 24:0 carbons did not show any significant correlation with the expression of the ACOX1 isoforms (Supplementary Figure S7).
Negative Regulation of Peroxisomal β-Oxidation Genes by DHA
Ligand-dependent regulations of PPARα and RXRα signaling have been tested using primary Acox1−/− hepatocytes. When exposed to DHA, Acox1−/− hepatocytes show a dramatic and a specific downregulation of L-PBE and PTL mRNAs in a dose-dependent manner (Figure 7a), and the western blot analysis also shows a decrease in L-PBE protein level (Figure 7b). Only 50 μM of DHA has a significant effect on CYP4A14 mRNA level (Figure 7a). Thus, increasing the DHA level in Acox1−/− hepatocytes, in which PPARα target genes are overexpressed, would lead to the specific activation of RXRα and recruitment of coactivators to the peroxisomal gene promoter (Figure 6a) leading to the negative regulation of the corresponding genes. This shows a feedback loop between the expression of peroxisomal β-oxidation genes involved in DHA synthesis and DHA-dependent activation of RXRα. On the other hand, treatment of Acox1−/− hepatocytes with nervonic acid (24:1) shows no significant effect on the mRNA levels of genes involved in the peroxisomal β-oxidation or microsomal ω-oxidation pathways (Figure 7a). However, western blot analysis shows that L-PBE protein level is slightly decreased by 24:1 treatment showing a possible posttranslational regulation by nervonic acid.
DISCUSSION
Fatty acids are metabolized in the liver by two distinct β-oxidation systems localized in the mitochondria and peroxisomes.3, 13, 16 The β-oxidation of medium and LCFAs occurs mainly in the mitochondria, whereas that of VLCFAs takes place exclusively in peroxisomes.13, 16 In addition, the microsomal ω-oxidation system, which includes the CYP4A10 and CYP4A14 enzymes, participates in the transformation of LCFAs and VLCFAs into DCAs, which are further β-oxidized in peroxisomes.16, 17 The mitochondria metabolize the bulk of dietary fatty acids for energetic purposes, whereas the extramitochondrial fatty acid oxidation systems in peroxisomes and endoplasmic reticulum only have a minor role in this respect.3, 13, 16 Accordingly, mice treated with tetradecylglycidic acid, an irreversible inhibitor of CPT1, a key enzyme in the control of mitochondrial β-oxidation, develop massive microvesicular hepatic steatosis.24 The mitochondrial fatty acid β-oxidation enzyme system abnormalities lead to disturbances in hepatic lipid metabolism with accumulation of lipid in hepatocytes.25, 26 It is also evident that the disruption of the peroxisomal Acox1 gene in the mouse affects hepatic lipid metabolism and contributes to the development of severe microvesicular hepatic steatosis.1, 3 The sustained activation of PPARα and the upregulation of PPARα target genes contribute to the hepatocellular regeneration with evidence of a spontaneous massive peroxisome proliferation leading to hepatocellular carcinomas.1, 2
In this study, we found that hepatic steatosis that develops in mice lacking Acox1 is attenuated upon expression of the human ACOX1b isoform and completely reversed upon expression of both the human ACOX1 isoforms 1a and 1b (Figure 1). The weaker reversal of the Acox1−/− phenotype by ACOX1a expression can be attributed at least in part to the weaker expression of ACOX1a after adenofection. Nevertheless, previous data from recombinant human ACOX1a characterization21 or expression in the fox1Δ deletion mutant of Saccharomyces cerevisiae22 have shown that ACOX1a is more labile and exhibits reduced activity toward palmitoyl-CoA as compared with ACOX1b. It is likely that ACOX1a functions as a heterodimer with ACOX1b, explaining conclusively why the expression of ACOX1a isoform with ACOX1b is more effective in the reversal of Acox1−/− phenotype. In these mice, several upregulated PPARα target genes coding for hepatic mitochondrial (MCAD, CPT-1a), microsomal (CYP4A10 and CYP4A14) and peroxisomal (L-PBE, PTL) proteins6, 13 were downregulated following the expression of human ACOX1 isoforms. This implies that the sustained hyperactivation of PPARα as caused by substrates which are left unmetabolized in the absence of ACOX1 and that function as PPARα ligands, are degraded upon expression of the human ACOX1 isoforms in the Acox1(−/−) mouse. In ACOX1 null mice, the accumulation in the liver of ACOX1 substrates, such as LCFAs, VLCFAs or their acyl-CoAs, triggered the spontaneous sustained PPARα hyperactivity analogous to that seen in the livers of wild-type mice exposed to synthetic peroxisome proliferators.13 In these mice, absence of peroxisomal fatty acid oxidation and induction of fatty acid ω-oxidation was found to lead to increased levels of DCAs that are toxic to the mitochondria, as they can uncouple oxidative phosphorylation.27 The expression of ACOX1b, or both ACOX1 isoforms reversed this process probably by degrading these natural PPARα ligands, thereby attenuating the sustained PPARα sensing. Reduced PPARα activation leads to a decrease in the generation of toxic DCAs by the extramitochondrial fatty acid oxidation pathway and minimizes mitochondrial damage leading to a reduction in hepatic steatosis. Support for the role of DCAs and PPARα hyperactivation is also derived from the observation that Acox1 and Pparα double nullizygous mice do not exhibit hepatic steatosis, implying that failure to induce CYP4A enzymes abrogates the production of these toxic derivatives.3, 13 This underscores the fact that hepatic steatosis developed in Acox1 null mice is strictly PPARα dependent. Treatment of Acox1(−/−) mice with synthetic peroxisome proliferators did not result in an additive induction of PPARα target genes, which suggests that natural ligands saturate PPARα in the Acox1−/− liver.3, 13 In these livers, analysis of PPARγ and PPARδ/β mRNAs levels showed no significant variations.
Analysis of circulating levels of unsaturated VLCFAs, regarded as PPARα agonists in vitro, showed that the expression of human ACOX1b alone, or in combination with ACOX1a, is accompanied by a significant increase in the level of nervonic acid (24:1), which is generated from the peroxisomal β-oxidation shortening of 26:1-CoA.16, 28 Recent data have shown that the CoA ester of nervonic acid (nervonyl-CoA) binds with high affinity to PPARα leading to a reduction in the recruitment of coactivators, such as CBP (cAMP response element-binding protein-binding protein).29 In addition, nervonic acid exerts a negative effect on the peroxisomal oxidation system30 and fails to activate PPARα.31 Hence, our findings point to the existence of an enzyme/product negative feedback mechanism in which nervonyl-CoA as produced by ACOX1b, a likely antagonist of PPARα. This may explain, why after expression of human ACOX1b, a reduction was found in the recruitment of PBP/Med1 and SRC-1 coactivators to response elements of PPARα target genes and their subsequent downregulation. Furthermore, recent data have shown that the monounsaturated fatty acid, C16:1n7-palmitoleate derived from de novo synthesis, decreases activation of PPARα target gene and suppresses hepatosteatosis.32, 33
In contrast to the expression of ACOX1b and increasing level of nervonic acid, the expression of both ACOX1 isoforms is accompanied by increased AA (20:4) and DHA (22:6) levels in serum of Acox1(−/−) mice. Interestingly, synthesis of DHA from its precursor tetracosahexanoic acid (24:6) is fully dependent on the functional peroxisomal β-oxidation pathway in both mouse and humans.16 Accordingly, the ChIP assay showed a unique profile of the L-PBE PPARα-response unit for which the recruitments of coactivators, PBP/Med1 and of SRC-1, are accompanied by a dominant recruitment of RXRα instead of PPARα. This is not surprising, as DHA activates RXRα in the adult mouse brain and promotes interaction between RXRα and SRC-1.34 Accordingly, the RXRα homodimer signaling pathway by PPAR-response units is fully functional and can rescue the severe hypothermia phenotype observed in fasted Ppar α−/− mice.35 Thus, ACOX1 isoforms produce the endogenous ligand of RXRα, the n-3 polyunsaturated DHA (22:6), which activates RXRα and the recruitment of coactivators (Figure 6e). As a universal hepatic integrator, RXRα heterodimerizes with several nuclear receptors, such as FXR, LXR, PPAR and TR, and thereby controls several metabolic pathways, including liver lipid metabolism.36 Interestingly, supplying Acox1−/− hepatocytes with DHA leads to a downregulation of peroxisomal β-oxidation pathway (Figure 7). This points to an original cross talk between RXRα and ACOX1, the former assuring the basal expression of ACOX1 activity and the latter taking care of the production of DHA, as a natural ligand of RXRα.
In summary, the observations described in this study clearly show that the human ACOX1b isoform reverses the Acox1 null mice fatty liver phenotype by reducing PPARα activation and its subsequent effects on fatty acids metabolism. Our data also point to a unique cross talk between the ACOX1 isoform and both PPARα and RXRα activations, thus implying a unique role of each of the two ACOX1 isoforms in controlling the intracellular levels of the endogenous ligands of PPARα and RXRα, respectively. Our data suggest that it may well be important to assess the relative levels of PPARα, RXRα and the ACOX1 isoforms in human nonalcoholic fatty liver disease, which is recognized as a leading cause of liver damage and cirrhosis in developed countries.
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Acknowledgements
Financial support for this study was received from a grant from the European Union project ‘Peroxisome’ LSHG-CT 2004–512018, The Institut National de la Santé et de la Recherche Médicale, the Regional Council of Burgundy, the Ministère de l'enseignement Supérieur et de la Recherche and from the National Iinstitut of Health GM 23750.
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Vluggens, A., Andreoletti, P., Viswakarma, N. et al. Functional significance of the two ACOX1 isoforms and their crosstalks with PPARα and RXRα. Lab Invest 90, 696–708 (2010). https://doi.org/10.1038/labinvest.2010.46
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DOI: https://doi.org/10.1038/labinvest.2010.46
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