Abstract
Cellular senescence, a stable proliferation arrest, is induced in response to various stresses. Oncogenic stress-induced senescence (OIS) results in blocked proliferation and constitutes a fail-safe program counteracting tumorigenesis. The events that enable a tumor in a benign senescent state to escape from OIS and become malignant are largely unknown. We show that lysyl oxidase activity contributes to the decision to maintain senescence. Indeed, in human epithelial cell the constitutive expression of the LOX or LOXL2 protein favored OIS escape, whereas inhibition of lysyl oxidase activity was found to stabilize OIS. The relevance of these in vitro observations is supported by in vivo findings: in a transgenic mouse model of aggressive pancreatic ductal adenocarcinoma (PDAC), increasing lysyl oxidase activity accelerates senescence escape, whereas inhibition of lysyl oxidase activity was found to stabilize senescence, delay tumorigenesis, and increase survival. Mechanistically, we show that lysyl oxidase activity favors the escape of senescence by regulating the focal-adhesion kinase. Altogether, our results demonstrate that lysyl oxidase activity participates in primary tumor growth by directly impacting the senescence stability.
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Main
Oncogene-induced senescence (OIS) drives cells into a stable cell cycle arrest, causing them to acquire specific markers (morphology, senescence-associated β-galactosidase activity (SA-β-Gal), for example) in response to aberrant oncogenic signals.1 In responsive cells, the stress generated by oncogene activation counterbalances the proliferation-stimulating potential of this activation by triggering senescence. Various benign tumors (melanoma nevi, prostatic intraepithelial neoplasias, lung adenomas) caused by oncogene activation accumulate senescent cells. The tumors remain in a benign state as long as senescence is sustained. Conversion to malignancy of senescing benign lesions thus involves escape from senescence.2, 3, 4
The p16-Rb and p53 pathways are key pathways in the regulation of OIS and senescence in general,1, 5, 6, 7, 8 but mounting evidence shows that senescence can occur without any involvement of either of these pathways. To date, little is known about these mechanisms.9, 10, 11, 12, 13, 14, 15 Most of the data demonstrating a central role of p53, its upstream activators, (DNA damage, p14ARF) and the p16-Rb pathway have been obtained with fibroblasts. Similar data generated in human epithelial cells (HECs) or other lineages are quite rare, and suggest a more complex picture of the genetic events involved in escape from senescence.15 Among primary HECs, primary human mammary epithelial cells probably constitute the best-characterized cell model. Post-stasis human mammary epithelial cells are unable to express p16INK4a,16 but they can still enter senescence, in a p53-independent manner in response to oncogenic stress.14 We have thus chosen this model to investigate alternative pathways involved in escape from OIS.
Lysyl oxidase activity (LOX), exerted by the LOX, LOXL1, LOXL2, LOXL3, and LOXL4 proteins,17, 18 regulates cell behavior by oxidizing lysine residues of their substrates and H2O2 production. LOX-family members are reported to exert both intracellular and extracellular effects, and to share or display specific activities.19, 20, 21, 22, 23 For example, LOX, LOXL1, and LOXL2 share the ability to promote migration, invasion, and metastasis and to regulate extracellular matrix organization.20, 24, 25, 26, 27, 28, 29, 30 How LOX activity affects cancer cell growth is still a matter of debate, as in some cases it is reported to have no effect24, 25 and in other cases to favor cancer cell growth19, 27, 28 in vitro or in vivo. Although both LOXL2 and LOXL3 seem to exert some specific effects on cancer by targeting the embryonic transcription factor Snail,22 some of the effects shared by LOXL2 and LOX are exerted via activation of the focal-adhesion kinase (FAK).19, 24, 27, 31, 32, 33
In the context of neu-induced breast tumorigenesis, importantly, LOX activity appears to favor tumorigenesis by regulating FAK.31 The oncogenic potential of neu, a tyrosine kinase receptor, can be limited by activation of the OIS fail-safe program.34 We thus hypothesized that LOX activity might affect oncogene-induced tumorigenesis by impacting the OIS fail-safe program triggered by oncogenic activation. We show that LOX activity is indeed a key regulator of OIS, thereby affecting tumorigenesis.
Results
An unstable OIS model based on HECs
To study OIS in HECs, we used post-stasis mammary HECs, which do not express p16INK4a.16 We reasoned that this extinction of p16INK4a would favor senescence instability, as demonstrated by other studies.35, 36 The cells were next immortalized by forced hTert expression (sustaining telomere integrity and thus avoiding replicative senescence16) and stably infected to express an inducible oncogene: MEK:ER (HEC-TM cells) or, in some experiments, RAF:ER (HEC-TR cells). In HEC-TM cells, where MEK was activated by a 3-day treatment with 4-hydroxytamoxifen (4-OHT) (Figure 1a), as indicated by increased phosphorylation of its substrate ERK (Figure 1b), we first checked that OIS was induced on d0. OIS induction by 4-OHT was demonstrated by loss of the two proliferation markers examined (cyclin A, a cyclin accumulated in the S to early M phases and PhosphoH3Ser10, a marker of late mitosis) (Figure 1b), growth arrest (Figure 1c), the appearance of the SA-β-Gal marker (Figure 1d) and an increase of the senescence markers IL85 and Sprouty237 (Figure 1e). As hypothesized, HEC-TM cells monitored after entry into senescence proved able to resume growth (Figure 1c) and to lose SA-β-Gal activity (Figure 1d) and other senescence markers (Figure 1e). This model is thus a suitable model for uncovering new mechanisms accelerating or inhibiting escape from OIS in HECs.
LOX activity regulates escape from OIS in HECs
To investigate the possible role of LOX activity in regulating senescence, we first used RT–qPCR to measure levels of transcripts corresponding to Lox-family proteins in HECs. LOX, LOXL1, and LOXL2 mRNAs were detected in these cells but LOXL3 and LOXL4 transcripts were not (Supplementary Figure 1). Cells were then infected with LOX- or LOXL2-encoding vectors, two main LOX-family members expressed in HEC, and their expression was checked by immunoblotting (Figure 2a). Cells constitutively expressing LOX, LOXL2, or neither of these were treated with 4-OHT to trigger senescence (d0), released from oncogenic stress for 3 days (d3), and then examined for escape from senescence (Figure 1a). On d3, although the initial growth arrest (d0) was similar in control, LOX-expressing, and LOXL2-expressing cells (Figure 2b), the LOX- and LOXL2-expressing cells displayed escape from OIS in contrast to control cells, that is, they were growing (Figure 2b), fewer of them were SA-β-Gal-positive (Figure 2c), and they displayed a decrease of senescence markers (Figure 2d). Thus, LOX and LOXL2 promote escape from OIS and this effect is not due to a simple growth advantage, as their constitutive expression does not affect cell growth in the absence of oncogenic stress induction (Figure 2e).
Secreted LOX favors escape from OIS in HECs
As LOX proteins are secreted, their production by various cells of the tumor microenvironment (such as fibroblasts or endothelial cells) might contribute, along with synthesis by the epithelial cells, to affecting the response to oncogenic stress. We thus examined whether extracellular LOX might impact OIS. We first measured LOX protein levels in the supernatants of constitutively expressing cells. As expected, LOX and LOXL2 were found in the supernatants (Figure 3a), LOX in its 30-kD mature form lacking the pro-domain and LOXL2 displaying the same size as in the lysate, as this protein contains no pro-domain.17, 18 Accordingly, the LOX and LOXL2 supernatants displayed increased LOX activity (Figure 3b). The results of colony assays (Figure 3c) and SA-β-Gal staining (Figure 3d) showed that adding LOX or LOXL2 supernatant to HEC-TM cells is sufficient to trigger escape from senescence. Importantly, LOX catalytic activity was found to be responsible for LOX-triggered escape from senescence: this escape was completely blocked upon addition of 3-aminopropionitrile (BAPN), an inhibitor of the catalytic activity of all the LOX proteins38, 39, 40 (Figures 3c and d), and a catalytically inactive LOX form proved unable to induce senescence reversal (Supplementary Figure 2).
Inhibiting LOX catalytic activity stabilizes OIS in HECs
Interestingly, HEC-TM cells displayed spontaneous escape from senescence after oncogenic stress and OIS induction (Figures 1 and 4a–b). As increasing LOX activity favors senescence escape, we next investigated whether this spontaneous escape might be inhibited by inhibiting any endogenous LOX activity due to LOX, LOXL1, and/or LOXL2 (Supplementary Figure 1). During oncogenic stress, LOX activity inhibition by BAPN treatment did not significantly affect entry into OIS (Figures 4b–d), but it did, strikingly, completely block spontaneous escape from OIS, as shown by (i) the inability of treated cells to grow, in contrast to control cells (Figure 4b), (ii) their ability to maintain SA-β-Gal activity (Figure 4c) and other senescence markers (Figure 4d) under conditions where untreated cells lost these markers. These effects were not due to direct inhibition of cell growth by LOX, as BAPN treatment alone did not modify HEC-TM cell growth (Figure 4e). This set of data was obtained by inhibiting LOX activity during oncogenic stress induction. Interestingly, inhibiting LOX activity when the cells were already senescent (d0) also stabilized senescence (Supplementary Figure 3), supporting the idea that LOX activity modifies escape from senescence but not entry into senescence.
LOX activity regulates OIS stability in vivo
To address the relevance of these interesting in vitro observations on HECs, we sought an appropriate mouse model that would be relevant to human cancer biology. We decided to focus on pancreatic ductal adenocarcinoma (PDAC), because (i) p16 is generally lost in human PDAC,41 and (ii) we observed LOX and LOXL2 induction (Supplementary Figure 4a) as well as some senescence (Supplementary Figure 4b) in a mouse model developing aggressive PDAC at the frequency of 100% by the age of 6–7 weeks as a result of pancreatic expression of an oncogenic Ras in a p16-null background (Pdx1-Cre, LSL-KrasG12D/+, INK4a/Arflox/lox42, 43). Wild-type (WT) and Pdx1-Cre, LSL-KrasG12D/+, INK4a/Arflox/lox (KIA) mice were killed 45 days after birth. The pancreases of KIA and WT animals were dissected and the normal zone was separated from the tumoral zone. In the KIA mice, the normal part (confirmed by hematoxylin-phloxine-saffron (HPS) staining) showed senescence (as indicated by the presence of SA-β-Gal activity) and tested negative for the proliferation marker Ki67 (Supplementary Figure 4b). In contrast, the tumoral part (confirmed by HPS staining) no longer displayed any SA-β-Gal activity and was Ki67-positive (Supplementary Figure 4b). The pancreases of WT animals displayed no SA-β-Gal activity and no Ki67 staining (Supplementary Figure 4b). KIA mice thus constitute an attractive model for testing OIS stability, and more specifically, the ability of LOX activity to regulate OIS in vivo.
To examine whether increased LOX activity might accelerate senescence, we first injected concentrated LOX protein supernatants (Figure 3) in mice. The LOX activity increase was observed in pancreatic extract for at least 4 h after intraperitoneal injection (Supplementary Figure 5). We then injected LOX protein supernatants into KIA mice, every day from days 23 to 38 after birth, and confirmed the LOX activity increase on the basis of better collagen fiber organization in the pancreatic tumor microenvironment 39 days after birth (Figure 5a). The SA-β-Gal (Figure 5b), Dec1 (Figure 5c) and Wnt16B (Figure 5d) senescence markers were all found to be lower and the Ki67 proliferation marker higher (Figure 5e) in mice injected with LOX supernatants than in mice injected with control supernatants. Hence, in agreement with the in vitro results, LOX activity can also accelerate escape from senescence in vivo in an aggressive model of PDAC.
We next examined whether LOX activity inhibition by BAPN might prevent the spontaneous escape from senescence observed 45 days after birth. The pancreases of BAPN-treated WT animals displayed no SA-β-Gal activity and no Ki67 staining (Supplementary Figure 4b), in agreement with the in vitro observation that BAPN cannot induce senescence in the absence of oncogenic stress (Figure 4e). BAPN injection into KIA mice resulted in the inhibition of pancreatic LOX catalytic activity, as measured by collagen fiber organization (Figure 6a). The pancreases of untreated mice were found to be SA-β-Gal-negative, whereas those of the BAPN-treated mice were SA-β-Gal-positive (Figure 6b) and displayed increased levels of the Dec1 and Wnt16B senescence markers (Figures 6c and d) and a decreased level of the Ki67 proliferation marker (Figure 6e). Importantly, LOX-inhibition-induced stabilization of senescence led to a significant increase in mouse survival (Figure 6f). Altogether, these results tally with our in vitro results and strongly support an involvement of LOX in regulating senescence stability, in vivo tumorigenesis, and survival.
LOX activity impacts OIS by modulating FAK activity
LOX activity is reported in various contexts to impact FAK activity.19, 24, 27, 31, 32, 33 We thus investigated in vitro and in vivo whether FAK might have a role in regulating senescence. In vitro, the level of P-FAK (FAKY397) was found to decrease during OIS (Figure 7a), and this decrease was sustained by LOX activity inhibition (Figure 7b). In vivo, the level of P-FAK was found to increase in the pancreases of LOX-injected KIA mice (Figure 7c) and to decrease in those of KIA mice when LOX activity was inhibited by BAPN treatment (Figure 7d).
That FAK signaling affects OIS stability is further supported by the observation that sub-cytostatic doses of two FAK inhibitors (FAK Inhibitor 14 and PF 573228) inhibited growth resumption (Figure 8a) and sustained senescence markers (Figures 8b and c). To determine whether FAK activation might allow cells to escape from 4-OHT/BAPN-induced irreversible senescence, we used HEC-TR cells expressing a constitutively active form of FAK (Figure 8d). After 4-OHT/BAPN treatment, the ability of these cells to form colonies (Figure 8e) and their decreased levels of senescence markers (Figures 8f and g) showed that they could escape from treatment-induced irreversible senescence even when LOX activity was inhibited. These data constitute compelling evidence that LOX activity influences OIS by regulating FAK signaling.
Discussion
Little is known about pathways, other than the p16-Rb and the p53 pathways, that might participate in controlling OIS. Here we show that LOX activity has a role in regulating escape from OIS, as this escape is triggered when LOX activity is increased by constitutive LOX or LOXL2 expression or by treating cells with secreted LOX or LOXL2.
Interestingly, HEC-TM cells show spontaneous escape from OIS. This instability of senescence might be due to loss of p16INK4a resulting from methylation of its promoter,16 as a decreased p16 level is reported to possibly favor escape from senescence.35, 36 This raises the question of whether the phenomenon we observed is really senescence. We think it is, because (i) the level of SA-β-Gal senescence marker increases, (ii) the levels of other independent senescence markers increase, and (iii) proliferation is blocked as long as the oncogenic stress is sustained. Interestingly, blocking LOX activity in this situation is sufficient to stabilize the growth arrest and the senescence markers and thus to maintain the senescent phenotype. Importantly, raising or lowering the LOX activity does not affect the proliferation of HEC cells. The growth-promoting effect of LOX activity is observed only in the context of OIS. In our in vivo model we have also observed a transient senescent phenotype, possibly due to the lack of p16INK4a expression, and again this phenotype is regulated by LOX activity.
LOX and LOXL2 are reported to be expressed both by epithelial cells and by cells of the tumor microenvironment.20, 31, 44 This means that these proteins, although expressed by different cells of different lineages, might impact epithelial cell behavior. To recapitulate the production of LOX and LOXL2 by tumor cells as well as cells of the tumor microenvironment, we have manipulated extracellular LOX and LOXL2 and described their impact on the epithelial cells response to the oncogenic stress.
Like other groups in the context of metastasis, primary tumor growth, or tumorigenesis, we observe an influence of FAK on biological responses to LOX activity.19, 24, 27, 31, 32, 33 We might speculate that the PI3K pathway is a downstream effector of the action of FAK on senescence, as it is known to be regulated by FAK45 and as it has recently been reported to inhibit senescence induction by activated RAS or RAF in mouse models of melanoma or pancreatic cancer.46, 47 How LOX activity activates FAK remains unclear. Some suggest that activation might be due to ECM stiffening, while others propose that it might be due to the hydrogen peroxide released by intrinsic LOX activity.19, 24, 27, 31, 32, 33
Altogether, our results support the view that the LOX activity, in addition to the p16INK4a pathway, regulates OIS and might thus affect tumorigenesis.
Materials and Methods
Cell culture
Mammary HECs (Lonza, Barcelona, Spain) were cultured in MEBM (Promocell, Heidelberg, Germany) and penicillin/streptomycin (Life Technologies, Saint Aubin, France). Virus-producing GP293 cells (Clontech, Saint-Germain-en-Laye, France) were cultured in DMEM (Life Technologies) supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA) and penicillin/streptomycin. Infected cells were selected, as appropriate, with neomycin (100 μg/ml), puromycin (500 ng/ml), or both.
Reagents and plasmids
Four-hydroxytamoxifen (4-OHT) (Sigma, Lyon, France) was used daily for 3 days at 250 nM final concentration. 3-Aminopropionitrile (fumarate salt) (A3134, Sigma) was used daily at 350 μ M final concentration for in vitro analysis. FAK inhibitor 14 (3414, Tocris Bioscience, Bristol, UK) and PF 573228 (3239, Tocris Bioscience) were used daily at the following respective final concentrations: 250 nM and 500 nM.
The following plasmids were used: pWZL-Neo-Myr-Flag-FAK (Addgene plasmid 20610, Cambridge, MA, USA),48 pBabe-hygro-hTert (Addgene plasmid 1773),49 pBAbe-puro-Raf:ER,50 pNLP-Neo-Mek:ER.3 For LOX and LOXL2 cloning, the C-terminally FLAG-tagged mouse LOX and LOXL2 cassettes were amplified by PCR with PFU (Agilent Technologies, Les Ulis, France)44 and introduced into the pLPCX vector (Clontech) between the NotI and ClaI sites. Catalytically inactive LOX was created by PCR site-directed mutagenesis to introduce the inactive mutations K314A and Y349F, as previously described51 and cloned into pLPCX vector as indicated above.
Transfection and infection
GP293 cells were transfected with PEI reagent according to the manufacturer’s recommendations (Euromedex, Souffelweyersheim, France). Two days after transfection, target HECs were infected with viral supernatant mixed with fresh medium (1/2) and polybrene (final concentration: 8 μg/ml).
Antibodies
The antibodies used were: anti-phosphoERK (9101, Cell Signaling, Danvers, MA, USA), anti-phosphoFAKTyr397 (3283, Cell Signaling, 44624G, Life Technologies), anti-FAK (3285, Cell Signaling), anti-flag (200472, Agilent Technologies), anti-phosphohistone3Ser10 (ab14955, Abcam, Paris, France), anti-cyclinA (H432, sc-751, Santa Cruz Biotechnology, Heidelberg, Germany), anti-Ki67 (clone Tec-3, M7249, DAKO, Les Ulis, France), and anti-tubulin (T6199, Sigma), WNT16B (LS-A9630, MBL, Nanterre, France).
Cell growth assays
Fifteen thousand cells were seeded into six-well plates and treated or not with the indicated compound(s). At the end of the experiments, cells were fixed with 4% PFA for 15 min, washed with water, and stained with a crystal violet solution (Sigma).
LOX activity assays
Activity assays for detection of BAPN-inhibitable LOX enzyme activity were performed with the Amplex Red Monoamine Oxidase Assay Kit (A12214, Molecular Probes, Life Technologies, Saint Aubin, France). Briefly, conditioned cell medium was concentrated with Amicon 10-kDa cutoff filters (Millipore, Darmstadt, Germany). Aliquots were added to the final reaction mix containing 100 μ M Amplex Red, 0.5 U/ml horseradish peroxidase, 2 mM benzylamine substrate and incubated for 1 h. The fluorescent product was excited at 560 nm and the emission was read at 590 nm. Parallel assays were prepared with 500 μ M BAPN to completely inhibit the activity of LOX, and the difference in emission intensity was recorded. Assays were run in quintuplicate and specific activity is reported as a mean of all assays.
RT-qPCR
Cells were lysed in TriReagent (Sigma) and total RNAs were isolated via an acidphenol extraction procedure using Phase Lock gel tubes (5′). RNA (2 μg) was reverse-transcribed with the First-Strand cDNA Synthesis Kit (GE Healthcare, Velizy-Villacoublay, France) according to the manufacturer’s directions. Q-PCR experiments were carried out in a Light Cycler 2.0 instrument, in Light Cycler Taqman Master Mix (Roche, Meylan, France) and with the Universal Probe Library (Roche Diagnostics, Meylan, France). The following primers were used: IL-8 5′-AGACAGCAGAGCACACAAGC-3′ and 5′-ATGGTTCCTTCCGGTGGT-3′, SPROUTY2 5′-TTTGCACATCGCAGAAAGAA-3′ and 5′-TCAGGTCTTGGAAGTGTGGTC-3′, LOX 5′-GGATACGGCACTGGCTACTT-3′ and 5′-GACGCCTGGATGTAGTAGGG-3′, LOXL1 5′-GCATGCACCTCTCATACCC-3′ and 5′-CAGTCGATGTCCGCATTGTA-3′, LOXL2 5′-TGACCTGCTGAACCTCAATG-3′ and 5′-TGGCACACTCGTAATTCTTCTG-3′. The actin gene was used as a normalizer, with the 5′-ATTGGCAATGAGCGGTTC-3′ and 5′-GGATGCCACAGGACTCCAT-3′ primers.
Mouse engineering and treatment
By crossing Pdx1-Cre;Ink4a/Arflox/lox (no phenotype) with LSL-KrasG12D/+; Ink4a/Arflox/lox (no phenotype) individuals, we ‘routinely’ generate Pdx1-Cre;LSL-KrasG12D/+; Ink4a/Arflox/lox animals (representing 25% of the total progeny according to the expected Mendelian inheritance) developing macroscopic pancreatic cancer at the frequency of 100% by the age of 6–7 weeks. Mice were treated by intraperitoneal injection of BAPN (100 mg/kg, dissolved in saline) or vehicle three times a week (half) or every day (half). Conditioned cell media from ctrl, LOX-expressing, or LOXL2-expressing cells were concentrated as described, and 100 μl of either ctrl supernatant or of a mix of 50 μl LOX supernatant and 50 μl LOXL2 supernatant was injected intraperitoneally every day for the indicated time. The experiments were performed in accordance with the animal care guidelines of the European Union and French laws and were validated by the local Animal Ethic Evaluation Committee (CECCAPP).
SA-ß-Gal analysis
Senescence-associated β-galactosidase activity was assayed in HECs and on pancreatic sections fixed and stained with the Senescence Beta-galactosidase Kit (Cell Signaling) as recommended by the manufacturer.
Histology and sirius red staining
Pancreatic sections (4–5 μm) were stained with HPS. For immunohistochemical analysis, paraffin-embedded murine pancreatic tumor tissues were used. Slides were serially sectioned at 4-μm thickness. After deparaffinization and rehydration, the slides were incubated in 5% hydrogen peroxide in sterile water to block endogenous peroxidases. For heat-induced antigen retrieval, tissue sections were boiled in 10 mmol/l citrate buffer pH6 in a microwave oven for 20 min. The slides were then incubated at room temperature for 1 hour with the primary antibody diluted in ‘low-background’ antibody diluent (DAKO Real). After rinsing in PBS, the slides were incubated with a biotinylated secondary antibody bound to a streptavidin peroxidase conjugate (Dako E0468) for 1 hour at room temperature. Bound antibody was revealed and sections were finally counterstained with hematoxylin.
Paraffin-treated sections of pancreas were stained for 1 hour in 0.1% picrosirius red solution (Direct Red 80, 365548, Sigma) in picric acid solution (P6744, Sigma), washed twice with acidified water, and then mounted in Eukitt quick-hardening mounting medium (03989, Sigma). Samples were analyzed upon polarized light microscopy.
Statistical analysis
The values are presented as mean±S.D. unless stated otherwise. Statistical analysis were performed using the Student’s t-test (*P<0.05, **<0.01, ***<0.001). The number of independent replicates for each experiment was indicated in the figure legends. Survival of the mice was analyzed using a Kaplan–Meier method. P-values were calculated using a log-rank test.
Abbreviations
- 4-OHT:
-
4-hydroxytamoxifen
- BAPN:
-
β-aminopropionitrile
- HEC:
-
human epithelial cells
- HEC-TM:
-
HEC–hTert–MEK:ER
- HEC-TR:
-
HEC–hTert–RAF:ER
- FAK:
-
focal-adhesion kinase
- LOX:
-
lysyl oxidase
- LOXL -1 -2 -3 -4:
-
LOX-Like (-1 -2 -3 -4)
- OIS:
-
oncogene-induced senescence
- PDAC:
-
pancreatic ductal adenocarcinoma
- SA-β-Gal:
-
senescence-associated β-galactosidase
- WT:
-
wild-type mice
- KIA:
-
Pdx1-Cre, LSL-KrasG12D/+, INK4a/Arflox/lox mice
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Acknowledgements
We thank members of the laboratory I Puisieux and P Sommer for helpful discussions, and we also thank S Léon, B Kaniewski, S Martel, and M Ferrand for technical assistance. This work was carried out with the support of the ‘RTRS Fondation Synergie Lyon Cancer’, the ‘Association pour la Recherche sur le Cancer’, the ‘Fondation de France’, and the ‘Institut National du Cancer’.
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Wiel, C., Augert, A., Vincent, D. et al. Lysyl oxidase activity regulates oncogenic stress response and tumorigenesis. Cell Death Dis 4, e855 (2013). https://doi.org/10.1038/cddis.2013.382
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DOI: https://doi.org/10.1038/cddis.2013.382
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