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| Open AccessEthnic-specific associations of rare and low-frequency DNA sequence variants with asthma
Common variants account for only a small amount of the heritable risk for developing asthma. Using a meta-analysis approach, Igartua et al. identify one low-frequency missense mutation and two genes with functional variants that are associated with asthma, but only in specific ethnic groups.
- Catherine Igartua
- , Rachel A. Myers
- & Carole Ober
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| Open AccessTargeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells
The microbial RNA-guided CRISPR/Cas9 system has robust genome-editing activities, but the off-target effects of the Cas9 nuclease have only recently begun to be analysed. Here the authors provide evidence for high specificity of the Cas9 nuclease on targeting of the Tafazzin gene in human-induced pluripotent stem cells and demonstrate the impact of single-nucleotide variations of the human genome on Cas9 specificity.
- Luhan Yang
- , Dennis Grishin
- & George Church
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Age-related variations in the methylome associated with gene expression in human monocytes and T cells
The functional relevance of age-related variation in DNA methylation is unclear. Here, Reynolds et al. analyze how patterns of genome-wide gene expression and DNA methylation data vary with age in circulating monocytes and T cells, and report age-associated methylation signals that are correlated with cis-gene expression and vascular aging.
- Lindsay M. Reynolds
- , Jackson R. Taylor
- & Yongmei Liu
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| Open AccessGenetic characterization of Greek population isolates reveals strong genetic drift at missense and trait-associated variants
Isolated populations can increase power to detect low frequency and rare risk variants associated with complex phenotypes. Here, the authors identify variants associated with haematological traits in two isolated Greek populations that would be difficult to detect in the general population, due to their low frequency.
- Kalliope Panoutsopoulou
- , Konstantinos Hatzikotoulas
- & Eleftheria Zeggini
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| Open AccessPharmacogenetic meta-analysis of genome-wide association studies of LDL cholesterol response to statins
Statins are effectively used to prevent and manage cardiovascular disease, but patient response to these drugs is highly variable. Here, the authors identify two new genes associated with the response of LDL cholesterol to statins and advance our understanding of the genetic basis of drug response.
- Iris Postmus
- , Stella Trompet
- & Chris C. A. Spencer
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| Open AccessFunctional annotation of colon cancer risk SNPs
Previous studies identified genetic variants associated with colorectal cancer (CRC), but the functional consequences of these genetic risk factors remain poorly understood. Here, the authors report that CRC risk variants reside in promoters and enhancers and could increase colon cancer risk through gene expression regulation.
- Lijing Yao
- , Yu Gyoung Tak
- & Peggy J. Farnham
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| Open AccessCommon variation near ROBO2 is associated with expressive vocabulary in infancy
The genetic basis of expressive vocabulary in children around 2 years old is poorly understood. Here, the authors show that a genetic variant near the ROBO2gene is associated with early language acquisition in the general population and highlight a potential genetic link between language-related common genetic variation and a linkage region for dyslexia, speech-sound disorder and reading.
- Beate St Pourcain
- , Rolieke A.M. Cents
- & George Davey Smith
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| Open AccessA rare variant in APOC3 is associated with plasma triglyceride and VLDL levels in Europeans
Population-based genome sequencing provides an increasingly rich resource for the identification of low-frequency, large effect variants associated with clinically important phenotypes. Timpson et al. use UK10K data to identify a variant of the APOC3gene strongly associated with plasma triglyceride levels.
- Nicholas J. Timpson
- , Klaudia Walter
- & Hou-Feng Zheng
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Immunochip SNP array identifies novel genetic variants conferring susceptibility to candidaemia
Candidaemia is a common cause of bloodstream infection, but the genetic basis of Candidainfection is poorly understood. Here, the authors identify genetic variation at three loci that increase risk of candidaemia, and show that genes at these loci have a role in antifungal host defence.
- Vinod Kumar
- , Shih-Chin Cheng
- & Mihai G. Netea
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| Open AccessA robust SNP barcode for typing Mycobacterium tuberculosis complex strains
Genetic variation in Mycobacterium tuberculosiscomplex (MTBC) bacteria is responsible for differences in factors such as virulence and transmissibility. Here, the authors analyse the genomes of 1,601 MTBC isolates from diverse geographic locations and identify 62 SNPs that may be used to resolve lineages and sublineages of these strains.
- Francesc Coll
- , Ruth McNerney
- & Taane G. Clark
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| Open AccessIdentification of genetic variants associated with alternative splicing using sQTLseekeR
RNA sequencing has enabled the global analysis of both gene expression levels and splicing events. Here, the authors develop a multivariate approach that is able to identify SNPs that influence splicing, and investigate the overlap of these with functional domains across the genome, including previously identified GWAS signals.
- Jean Monlong
- , Miquel Calvo
- & Roderic Guigó
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Reduced selection and accumulation of deleterious mutations in genes exclusively expressed in men
Differential selection on sex-specific genes facilitates propagation of deleterious mutations. Here, the authors show that genes exclusively expressed in human testes harbour a greater number of deleterious mutations than genes expressed in both sexes, due to reduced selection in females.
- Moran Gershoni
- & Shmuel Pietrokovski
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Genome-wide analysis in Drosophila reveals age-specific effects of SNPs on fitness traits
Senescence is the decline in physiological function associated with age, and the genetic basis of this phenomenon is poorly understood. Here, the authors identify genetic variants in Drosophilathat affect reproduction and lifespan in an age-specific manner, and provide support for the mutation accumulation theory of aging.
- Mary F. Durham
- , Michael M. Magwire
- & Jeff Leips
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| Open Access2q36.3 is associated with prognosis for oestrogen receptor-negative breast cancer patients treated with chemotherapy
Studies have shown that breast cancer prognosis is hereditary. Here the authors show that a genetic variant in CCL20, a chemokine ligand involved in immune response, is significantly associated with breast cancer survival and may therefore represent an important therapeutic or prognostic target.
- Jingmei Li
- , Linda S. Lindström
- & Kamila Czene
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| Open AccessA barcode of organellar genome polymorphisms identifies the geographic origin of Plasmodium falciparum strains
Tracing the source of malarial infections is an important step towards monitoring and controlling the disease. Here, Preston et al. analyse sequence data from 711 isolates and design a genetic barcode based on combined mitochondrial and apicoplast genomes that is able to distinguish between malaria parasites isolated from different geographical regions.
- Mark D. Preston
- , Susana Campino
- & Taane G. Clark
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Characterizing the genetic basis of methylome diversity in histologically normal human lung tissue
The effects of genetic variation on DNA methylation patterns are poorly understood. Here, Shi et al.systematically map methylation-quantitative trait loci in lung, breast and kidney tissue to reveal the impact of inherited variation on the human methylome, which also affects cancer risk.
- Jianxin Shi
- , Crystal N. Marconett
- & Maria Teresa Landi
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| Open AccessA rare functional cardioprotective APOC3 variant has risen in frequency in distinct population isolates
Isolated populations may empower genetic association studies of complex traits. Here, the authors identify a rare cardioprotective APOC3variant in a Greek population isolate and highlight the value of using population isolates to detect rare variants that confer disease risk.
- Ioanna Tachmazidou
- , George Dedoussis
- & Eleftheria Zeggini
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Val66Met polymorphism of BDNF alters prodomain structure to induce neuronal growth cone retraction
The Val66Met single-nucleotide polymorphism in the BDNF gene is implicated in neuropsychiatric disorders. Anastasia et al.show that this polymorphism results in structural changes in the brain-derived neurotrophic factor prodomain, and growth cone retraction in the hippocampal neurons.
- Agustin Anastasia
- , Katrin Deinhardt
- & Clay Bracken
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Genetic integration of local dispersal and exploratory behaviour in a wild bird
The distance that individuals disperse to between birth and first breeding determines the dynamics and genetic composition of natural populations. Here the authors show that in great tits (Parus major), the distance of local dispersal is genetically coupled with exploratory behaviour measured in the lab.
- Peter Korsten
- , Thijs van Overveld
- & Erik Matthysen
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Genome architecture is a selectable trait that can be maintained by antagonistic pleiotropy
The contribution of chromosomal rearrangements to fitness remains to be directly quantified. By constructing rearrangements in fission yeast, the authors show that the resulting defects in meiosis may be compensated for by a strong growth advantage in mitosis.
- Ana Teresa Avelar
- , Lília Perfeito
- & Miguel Godinho Ferreira
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Genome-wide deserts for copy number variation in vertebrates
Uncovering the factors that influence the generation of copy number variation (CNV) across the genome may increase our understanding of the role of CNVs in disease. Here, Makino et al.provide insights into the mechanisms underlying CNV formation and suggest a method for identifying disease-associated CNVs.
- Takashi Makino
- , Aoife McLysaght
- & Masakado Kawata
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Genome-wide search for exonic variants affecting translational efficiency
Genetic effects on gene expression by variants at expression quantitative trait loci (eQTLs), can contribute to human genetic diseases. Here, Liet al. present a method to study eQTLs with effects on protein translation on a transcriptome-wide scale.
- Quan Li
- , Angeliki Makri
- & Hui-Qi Qu
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| Open AccessAnalysis of elite variety tag SNPs reveals an important allele in upland rice
Quantitative trait loci mapping has traditionally been used to discover desirable alleles in plants. In this study, Lyu et al. use a comparative genome approach to identify an allele that is prevalent in upland rice varieties and alters the function of a protein involved in abscisic acid biosynthesis.
- Jun Lyu
- , Shilai Zhang
- & Wen Wang
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| Open AccessInterferon-induced transmembrane protein-3 genetic variant rs12252-C is associated with severe influenza in Chinese individuals
A variant in the IFITM3gene increases the risk of severe influenza, but homozygosity is rare in Caucasians. The authors show that the variant gene is homozygous in 25% of healthy Chinese people, and 69% of those with severe pandemic influenza, suggesting that this gene influences the epidemiology of influenza in South-East Asia.
- Yong-Hong Zhang
- , Yan Zhao
- & Tao Dong
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A fast and accurate SNP detection algorithm for next-generation sequencing data
The amount of data supplied by next-generation sequencing technologies presents a challenge for traditional algorithms to detect single-nucleotide polymorphisms. Xu et al.develop an efficient detection program and demonstrate its utility by identifying polymorphisms in cancer genomes and human populations.
- Feng Xu
- , Weixin Wang
- & Junwen Wang
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| Open AccessEvolution of the human-specific microRNA miR-941
miRNAs simultaneously regulate a range of genes, making them potential master players in evolution. Huet al.identify a human-specific miRNA called miR-941, whose copy number remains polymorphic in modern humans, and show that miR-941 is expressed in the brain and could regulate important signalling pathways.
- Hai Yang Hu
- , Liu He
- & Philipp Khaitovich
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| Open AccessThe genetic prehistory of southern Africa
Hunter-gatherer populations in Africa preserve unique information about human history, but genetic sub-structures of these populations remain unclear. Using newly designed microarray and statistical methods, these authors analyse genetic compositions of southern African populations and reveal an ancient link between southern and eastern Africa.
- Joseph K. Pickrell
- , Nick Patterson
- & Brigitte Pakendorf
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| Open AccessEmergence of stable polymorphisms driven by evolutionary games between mutants
Mutation, selection and random drift determine evolutionary dynamics and can give rise to polymorphisms. Here, an evolutionary game model, in which each new mutation generates a new evolutionary game, is applied to study the emergence of polymorphism, resulting in higher diversity than seen in previous models.
- Weini Huang
- , Bernhard Haubold
- & Arne Traulsen
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| Open AccessThe global distribution of the Duffy blood group
The global prevalence of the Duffy blood group variants is important due to the resistance that the Duffy-negative phenotype generally confers uponPlasmodium vivax infection. Hay et al.generate global frequency maps of the common Duffy alleles to show transmission patterns of the malaria parasite.
- Rosalind E. Howes
- , Anand P. Patil
- & Simon I. Hay
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Larger mitochondrial DNA than Y-chromosome differences between matrilocal and patrilocal groups from Sumatra
Matrilocal and patrilocal populations are predicted to have greater genetic diversity in mitochondrial DNA and the Y-chromosome, respectively. Here, no difference in the diversity of the Y-chromosome was found in two such groups, suggesting that local diversity was caused by male gene flow in expanding populations.
- Ellen Dröfn Gunnarsdóttir
- , Madhusudan R. Nandineni
- & Mark Stoneking
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| Open AccessDeep resequencing reveals excess rare recent variants consistent with explosive population growth
To fully catalogue rare genetic variation in humans, many samples need to be examined. In this study, Coventryet al. resequenced two genes, KCNJ11 and HHEX, in 13,715 humans, and concluded that most of the sequence variation arose recently and that variation is greater than expected.
- Alex Coventry
- , Lara M. Bull-Otterson
- & Charles F. Sing