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| Open AccessA highly efficient non-viral process for programming mesenchymal stem cells for gene directed enzyme prodrug cancer therapy
- Yoon Khei Ho
- , Jun Yung Woo
- & Heng-Phon Too
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| Open AccessGenome editing with the donor plasmid equipped with synthetic crRNA-target sequence
- Riki Ishibashi
- , Kota Abe
- & Fumiko Toyoshima
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| Open AccessOptimizing bacteriophage engineering through an accelerated evolution platform
- Andrew H. Favor
- , Carlos D. Llanos
- & Jorge A. Bardales
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| Open AccessRapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing
- Yuji Yokouchi
- , Shinichi Suzuki
- & Takumi Era
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| Open AccessA novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus
- N. V. Bogert
- , J. Furkel
- & M. H. Konstandin
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| Open AccessEvaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio)
- Gabriela T. Rassier
- , Tony L. R. Silveira
- & Vinicius Farias Campos
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| Open AccessExpression and function of an Hac1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae
- Changjie Bao
- , Jiping Li
- & Sitong Zhang
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| Open AccessTranscriptional repression of PTEN in neural cells using CRISPR/dCas9 epigenetic editing
- C. Moses
- , S. I. Hodgetts
- & A. R. Harvey
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| Open AccessPropofol suppresses hepatocellular carcinoma by inhibiting NET1 through downregulating ERK/VEGF signaling pathway
- Guoxiong Fei
- , Meili Cao
- & Longjiu Cui
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| Open AccessAAV8 Ins1-Cre can produce efficient β-cell recombination but requires consideration of off-target effects
- Adam Ramzy
- , Eva Tudurí
- & Timothy J. Kieffer
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| Open AccessCRISPR-Cas9 generated Pompe knock-in murine model exhibits early-onset hypertrophic cardiomyopathy and skeletal muscle weakness
- Jeffrey Y. Huang
- , Shih-Hsin Kan
- & Raymond Y. Wang
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| Open AccessEfficient correction of a deleterious point mutation in primary horse fibroblasts with CRISPR-Cas9
- Carlos Pinzon-Arteaga
- , Matthew D. Snyder
- & Charles R. Long
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| Open AccessExploring the fitness benefits of genome reduction in Escherichia coli by a selection-driven approach
- Viktor Vernyik
- , Ildikó Karcagi
- & György Pósfai
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| Open AccessOtoferlin gene editing in sheep via CRISPR-assisted ssODN-mediated Homology Directed Repair
- A. Menchaca
- , P. C. dos Santos-Neto
- & M. Crispo
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| Open AccessOverexpression of V-type H+ pyrophosphatase gene EdVP1 from Elymus dahuricus increases yield and potassium uptake of transgenic wheat under low potassium conditions
- Yongbin Zhou
- , Yan Li
- & Ming Chen
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| Open AccessThe position of the target site for engineered nucleases improves the aberrant mRNA clearance in in vivo genome editing
- Jae Hoon Lee
- , Sungsook Yu
- & Han-Woong Lee
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| Open AccessEpistatic Effect of Regulators to the Adaptive Growth of Escherichia coli
- Yukari Miyake
- & Kaneyoshi Yamamoto
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| Open AccessThalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4
- David G. Belair
- , Gang Lu
- & Kyle L. Kolaja
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| Open AccessEnhanced homologous recombination by the modulation of targeting vector ends
- Shinji Hirotsune
- , Hiroshi Kiyonari
- & Fumio Matsuzaki
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| Open AccessA dog oviduct-on-a-chip model of serous tubal intraepithelial carcinoma
- Marcia de Almeida Monteiro Melo Ferraz
- , Jennifer Beth Nagashima
- & Nucharin Songsasen
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| Open AccessProduction of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9
- Takayuki Sakurai
- , Akiko Kamiyoshi
- & Takayuki Shindo
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| Open AccessA multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells
- Dane Z. Hazelbaker
- , Amanda Beccard
- & Lindy E. Barrett
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| Open AccessIndel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon
- Anne Hege Straume
- , Erik Kjærner-Semb
- & Rolf Brudvik Edvardsen
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| Open AccessEfficient tissue-type specific expression of target genes in a tetracycline-controlled manner from the ubiquitously active Eef1a1 locus
- Kazuhito Sakamoto
- , Patrick D. Rädler
- & Kay-Uwe Wagner
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| Open AccessEstablishment of sperm cryopreservation and in vitro fertilisation protocols for rats
- Naomi Nakagata
- , Nobuyuki Mikoda
- & Toru Takeo
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| Open AccessA reporter mouse for non-invasive detection of toll-like receptor ligands induced acute phase responses
- Chun-Fang Huang
- , Shang-Yi Chiu
- & Yu-Chia Su
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| Open AccessCell-based interferon gene therapy using proliferation-controllable, interferon-releasing mesenchymal stem cells
- Mari Tsujimura
- , Kosuke Kusamori
- & Makiya Nishikawa
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| Open AccessIdentification of cell context-dependent YAP-associated proteins reveals β1 and β4 integrin mediate YAP translocation independently of cell spreading
- Joanna Y. Lee
- , Antonia A. Dominguez
- & Ovijit Chaudhuri
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| Open AccessEfficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
- Isidre Hooghvorst
- , Camilo López-Cristoffanini
- & Salvador Nogués
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| Open AccessAn efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cells
- Takahito Ohira
- , Koichi Miyauchi
- & Hiroyuki Kugoh
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| Open AccessCRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)
- Alex K. Datsomor
- , Rolf E. Olsen
- & Per Winge
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| Open AccessA human expression system based on HEK293 for the stable production of recombinant erythropoietin
- Christine Lin Chin
- , Justin Bryan Goh
- & Say Kong Ng
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| Open AccessImproved Cas9 activity by specific modifications of the tracrRNA
- Tristan Scott
- , Ryan Urak
- & Kevin V. Morris
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| Open AccessCRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner
- Miki Hanasaki
- & Hiroshi Masumoto
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| Open AccessStrategies to reduce genetic mosaicism following CRISPR-mediated genome edition in bovine embryos
- I. Lamas-Toranzo
- , B. Galiano-Cogolludo
- & P. Bermejo-Álvarez
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| Open AccessHigh-resolution transcriptional profiling of Anopheles gambiae spermatogenesis reveals mechanisms of sex chromosome regulation
- Chrysanthi Taxiarchi
- , Nace Kranjc
- & Roberto Galizi
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| Open AccessSimple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation
- Satoru Iwata
- , Hitomi Nakadai
- & Takashi Iwamoto
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| Open AccessIdentification of the Sex of Pre-implantation Mouse Embryos Using a Marked Y Chromosome and CRISPR/Cas9
- Xiuling Zhao
- , Wei Wei
- & Ming Zhang
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| Open AccessTime origin and structural analysis of the induced CRISPR/cas9 megabase-sized deletions and duplications involving the Cntn6 gene in mice
- Inna E. Pristyazhnyuk
- , Julia Minina
- & Oleg L. Serov
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| Open AccessIn vivo genome editing using the Cpf1 ortholog derived from Eubacterium eligens
- Woo-Chan Ahn
- , Kwang-Hyun Park
- & Eui-Jeon Woo
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| Open AccessTargeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
- Kentaro Higuchi
- , Yukinori Kazeto
- & Koichiro Gen
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| Open AccessA novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
- Elizabeth C. Bryda
- , Hongsheng Men
- & Miriam A. Hankins
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| Open AccessGeneration of genetically modified mice using SpCas9-NG engineered nuclease
- Wataru Fujii
- , Haruka Ito
- & Kunihiko Naito
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| Open AccessEfficient derivation of knock-out and knock-in rats using embryos obtained by in vitro fertilization
- Arata Honda
- , Ryoma Tachibana
- & Masahide Asano
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| Open AccessOptimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues
- Takahiko Matsuda
- & Izumi Oinuma
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| Open AccessGenome analysis and genetic transformation of a water surface-floating microalga Chlorococcum sp. FFG039
- Yoshiaki Maeda
- , Daisuke Nojima
- & Tsuyoshi Tanaka
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| Open AccessDevelopment of latent Interferon alpha 2b as a safe therapeutic for treatment of Hepatitis C virus infection
- Iram Gull
- , Muhammad Shahbaz Aslam
- & Muhammad Amin Athar
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| Open AccessLiver-specific androgen receptor knockout attenuates early liver tumor development in zebrafish
- Hankun Li
- , Yan Li
- & Zhiyuan Gong
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| Open AccessBindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase
- Takayuki Sakurai
- , Akiko Kamiyoshi
- & Takayuki Shindo