News |
Featured
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Letter |
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
Utility of the small Staphylococcus aureus Cas9 is improved by broadening its targeting range through molecular evolution of its PAM sequence.
- Benjamin P Kleinstiver
- , Michelle S Prew
- & J Keith Joung
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Editorial |
Standing up for science
Smear campaigns against those speaking out against scaremongering on genetically modified (GM) crops highlight why support for scientists involved in public outreach is so important.
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News Feature |
Gene drive overdrive
The recent publication of a simple procedure for creating a CRISPR-Cas9-mediated gene drive has some researchers sounding the alarm. What are the risks to populations in the wild and what precautions are necessary? Laura DeFrancesco investigates.
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Brief Communication |
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease
Varying the length of sgRNAs allows knocking out and activating different genes in the same cell using catalytically active Cas9.
- James E Dahlman
- , Omar O Abudayyeh
- & Silvana Konermann
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Letter |
Photoactivatable CRISPR-Cas9 for optogenetic genome editing
The genome editing activity of CRISPR-Cas9 can be switched on and off by light using split Cas9 fragments fused tophotoinducible dimerization domains.
- Yuta Nihongaki
- , Fuun Kawano
- & Moritoshi Sato
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Correspondence |
The ethics of publishing human germline research
- Arun Sharma
- & Christopher Thomas Scott
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Letter |
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway.
- Van Trung Chu
- , Timm Weber
- & Ralf Kühn
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Letter |
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining
The efficiency of homologous recombination-based Cas9 genome editing is increased by inhibiting non-homologous end joining.
- Takeshi Maruyama
- , Stephanie K Dougan
- & Hidde L Ploegh
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Article |
Multiplexed tracking of combinatorial genomic mutations in engineered cell populations
An approach called TRACE enables mapping of millions of mutations in engineered cell populations.
- Ramsey I Zeitoun
- , Andrew D Garst
- & Ryan T Gill
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Editorial |
Kicking the can
European politicians' decision to kick the can of genetically modified (GM) crop approvals down to national governments may accelerate adoption in the short term, but foreshadows legal battles down the road.
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Correspondence |
A split-Cas9 architecture for inducible genome editing and transcription modulation
- Bernd Zetsche
- , Sara E Volz
- & Feng Zhang
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Brief Communication |
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
Off-target cleavage by CAS9 or TALEN genome editing tools is detected by integrase-defective lentiviral vectors.
- Xiaoling Wang
- , Yebo Wang
- & Jiing-Kuan Yee
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Correspondence |
Genetically engineered crops that fly under the US regulatory radar
- Alex Camacho
- , Allen Van Deynze
- & Alan B Bennett
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Article |
Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo
Efficient protein delivery using cationic lipid transfection reagents enables high efficiency protein-based genome editing in vivo and in vitro.
- John A Zuris
- , David B Thompson
- & David R Liu
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Letter |
Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
Delivery of CRISPR-Cas nucleases using bacteriophage enables targeted killing of microbes in a population
- Robert J Citorik
- , Mark Mimee
- & Timothy K Lu
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Correspondence |
Field trial of Xanthomonas wilt disease-resistant bananas in East Africa
- Leena Tripathi
- , Jaindra Nath Tripathi
- & Wilberforce Kateera Tushemereirwe
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Article |
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
A fusion of the FokI nuclease and a catalytically inactive Cas9 is a highly specific genome editing tool.
- John P Guilinger
- , David B Thompson
- & David R Liu
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Article |
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Genome-wide analysis of Cas9-DNA interactions in mammalian cells shows widespread binding but low levels of cleavage.
- Xuebing Wu
- , David A Scott
- & Phillip A Sharp
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Editorial |
Genome editing for all
CRISPR-Cas is about to transform how we interrogate genetic variants and model disease.
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News Feature |
Gene editing at CRISPR speed
Ease of use, economy and speed of targeting DNA has propelled the CRISPR-Cas system into the spotlight. Now, despite numerous gaps in our knowledge, commercial entities are looking for a piece of the action. Monya Baker reports.
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Review Article |
CRISPR-Cas systems for editing, regulating and targeting genomes
- Jeffry D Sander
- & J Keith Joung
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Article |
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library
CRISPR-Cas9 genome editing technology is used for genome-wide genetic screens in mouse embryonic stem cells.
- Hiroko Koike-Yusa
- , Yilong Li
- & Kosuke Yusa
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Letter |
Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins
A fusion protein comprising a TALE DNA-targeting domain and the 5-methylcytosine hydroxylase TET1 enables investigation of the function of specific DNA methylation events.
- Morgan L Maeder
- , James F Angstman
- & J Keith Joung
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Letter |
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
In vitro selection and high-throughput sequencing measure the sequence specificity of cleavage by gRNA-Cas9 complexes.
- Vikram Pattanayak
- , Steven Lin
- & David R Liu
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Correspondence |
Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems
- Wei Li
- , Fei Teng
- & Qi Zhou
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Correspondence |
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease
- Vladimir Nekrasov
- , Brian Staskawicz
- & Sophien Kamoun
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News & Views |
Taking the fish out of fish oil
Extensive metabolic engineering of the yeast Yarrowia lipolytica results in a sustainable and commercially viable alternative to fish oil for the supply of eicosapentaenoic acid.
- James P Wynn
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Correspondence |
Heritable gene targeting in the mouse and rat using a CRISPR-Cas system
- Dali Li
- , Zhongwei Qiu
- & Mingyao Liu
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News & Views |
A caffeine fix for human nuclear transfer?
Efficient human nuclear-transfer embryo cloning is here at last, prompting renewed efforts to explore hidden variables in the cloning process.
- Anthony C F Perry
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Letter |
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
A screen in human cells defines the targeting specificities of sgRNA:Cas9 and TAL-based transcriptional activators.
- Prashant Mali
- , John Aach
- & George M Church
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Letter |
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
CAS9 nucleases, a new tool for genome editing, show significant offtarget activity.
- Yanfang Fu
- , Jennifer A Foden
- & Jeffry D Sander
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Brief Communication |
TALEN-mediated editing of the mouse Y chromosome
Targeted nucleases succeed in modifying the Y chromosome that has been recalcitrant to traditional genome engineering methods.
- Haoyi Wang
- , Yueh-Chiang Hu
- & Rudolf Jaenisch
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News & Views |
RNA guides genome engineering
The Cas9 endonuclease is reprogrammed by RNAs for site-specific modification of eukaryotic and microbial genomes.
- Claudio Mussolino
- & Toni Cathomen
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Brief Communication |
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
The genome of human cells is edited using the bacterial RNA-guided Cas9 endonuclease.
- Seung Woo Cho
- , Sojung Kim
- & Jin-Soo Kim
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Brief Communication |
Efficient genome editing in zebrafish using a CRISPR-Cas system
A CRISPR-Cas system allows editing of the genome in zebrafish embryos.
- Woong Y Hwang
- , Yanfang Fu
- & J Keith Joung
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Article |
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
A CRISPR-Cas system is harnessed to introduce template-driven mutations in S. pneumoniae and E. coli at high efficiency without requiring selectable markers.
- Wenyan Jiang
- , David Bikard
- & Luciano A Marraffini