Fluorescent probes

Fluorescent probes are molecules that absorb light of a specific wavelength and emit light of a different, typically longer, wavelength (a process known as fluorescence), and are used to study biological samples. The molecules, also known as fluorophores, can be attached to a target molecule and act as a marker for analysis with fluorescence microscopy.

Latest Research and Reviews

News and Comment

  • News and Views |

    For decades chemists have focused on increasing the brightness of fluorophores. In super-resolution microscopy, however, fluorophores that preferentially exist in a non-fluorescent state, but occasionally re-arrange into a fluorescent form, can give better results.

    • Gražvydas Lukinavičius
    •  & Kai Johnsson
    Nature Chemistry 6, 663-664
  • News and Views |

    Tuning the luminescence lifetimes of upconversion nanocrystals through lanthanide doping provides new opportunities for optical multiplexing in the time domain for applications in imaging and security marking.

    • Renren Deng
    •  & Xiaogang Liu
    Nature Photonics 8, 10-12
  • Comments and Opinion |

    NCI and University of Tokyo researchers have developed a fluorescent probe that can be sprayed onto tissue during surgery to detect small metastases. The researchers plan to validate the method in fresh surgical tissue from ovarian cancer patients in preparation for an IND.

    • Joanne Kotz
  • News and Views |

    Conjugation of a known, mechanism-based glycosidase inhibitor to sensitive fluorophores yielded unexpectedly potent and selective probes for quantifying active lysosomal glucocerebrosidase. These conjugates could prove to be invaluable tools for diagnosing and studying Gaucher disease.

    • Ethan D Goddard-Borger
    • , Tom Wennekes
    •  & Stephen G Withers