Research Highlights |
Featured
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Methods in Brief |
Bacterial ghosts for cryo-electron tomography
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Method to Watch |
Super-resolution CLEM
Correlated light and electron microscopy (CLEM) is particularly powerful when applied in super-resolution.
- Natalie de Souza
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Brief Communication |
High-resolution structure determination by continuous-rotation data collection in MicroED
High-resolution, three-dimensional protein structures can be solved using MicroED, an electron diffraction method that uses three-dimensional microcrystals. An improved MicroED data collection approach described here increases data quality and resolution and extends its broad applicability.
- Brent L Nannenga
- , Dan Shi
- & Tamir Gonen
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Tools in Brief |
Displaying membrane proteins for cryo-EM
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Brief Communication |
Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography
This paper combines cryo-electron tomography with super-resolution fluorescence microscopy for precise localization of molecular tags on a cellular or macromolecular structure, without fixation.
- Yi-Wei Chang
- , Songye Chen
- & Grant J Jensen
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Brief Communication |
Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas
Graphene is in many ways an ideal sample support for cryo-electron microscopy, but its hydrophobicity prevents adsorption of protein from aqueous solution. Low-energy hydrogen-plasma treatment renders graphene hydrophilic and enables controlled adsorption of protein to its surface.
- Christopher J Russo
- & Lori A Passmore
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Article |
Cryo-scanning transmission electron tomography of vitrified cells
Cryo-scanning transmission electron tomography (CSTET) of unstained, fully hydrated vitrified biological specimens is shown to have advantages over cryo-electron tomography (CET), notably at high sample tilts providing greater depth resolution for thick samples.
- Sharon Grayer Wolf
- , Lothar Houben
- & Michael Elbaum
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Method to Watch |
Single-particle electron cryomicroscopy
Single-particle electron cryomicroscopy reaches for atomic resolution.
- Allison Doerr
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Research Highlights |
Electron crystallography goes 3D with MicroED
Electron diffraction using three-dimensional (3D) crystals may expand the reach of this technique.
- Allison Doerr
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Brief Communication |
Quantifying the local resolution of cryo-EM density maps
A method and software tool, ResMap, for quantifying the local resolution across 3D electron cryo-microscopy density maps is reported.
- Alp Kucukelbir
- , Fred J Sigworth
- & Hemant D Tagare
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News & Views |
Stroboscopic imaging of macromolecular complexes
Two reports describing the use of direct-conversion electron detectors and algorithms that correct for beam-induced sample motion in single-particle electron cryomicroscopy demonstrate that this technique can solve structures of macromolecules at near-atomic resolution.
- Robert M. Glaeser
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Article |
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM
The combination of a direct electron-detection camera that can count individual electrons and an algorithm for correcting for beam-induced motion in cryo-EM will facilitate determination of three-dimensional structures of smaller, lower-symmetry macromolecular complexes to higher resolution than previously possible.
- Xueming Li
- , Paul Mooney
- & Yifan Cheng
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Correspondence |
Prevention of overfitting in cryo-EM structure determination
- Sjors H W Scheres
- & Shaoxia Chen