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Article
| Open AccessStructural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome
The COP9 signalosome (CSN) regulates Cullin-RING Ligase 2 (CRL2) but the molecular basis for their interaction is unknown. Here the authors use structural mass spectrometry and cryo-EM approaches to assess the structures and dynamics of CSN-CRL2 complexes.
- Sarah V. Faull
- , Andy M. C. Lau
- & Argyris Politis
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Article
| Open AccessCognitive control of complex motor behavior in marmoset monkeys
Whether marmosets can exhibit complex motor tasks in controlled experimental designs has not yet been demonstrated. Here, the authors show that marmoset monkeys can be trained to call on command in controlled operant conditioning tasks.
- Thomas Pomberger
- , Cristina Risueno-Segovia
- & Steffen R. Hage
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Article
| Open AccessInhibition of amyloid beta toxicity in zebrafish with a chaperone-gold nanoparticle dual strategy
Treating Alzheimer’s disease, one of the most common neurodegenerative diseases, is of wide interest. Here, the authors report on the development of casein coated gold nanoparticles which were able to cross the blood brain barrier and protect against amyloid beta toxicity in a zebrafish model.
- Ibrahim Javed
- , Guotao Peng
- & Sijie Lin
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Article
| Open AccessMultifunctional multi-shank neural probe for investigating and modulating long-range neural circuits in vivo
Microelectromechanical neural probes can cause tissue damage and often cannot record from distant brain areas. Here the authors combine electrical recording, optical stimulation and microfluidic drug delivery in one multi-shank probe with thinner shanks to reduce damage and a flexible design to target long-range neural circuits.
- Hyogeun Shin
- , Yoojin Son
- & Il-Joo Cho
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Article
| Open AccessTuberculous meningitis in children is characterized by compartmentalized immune responses and neural excitotoxicity
Tuberculosis meningitis (TBM) is a severe form of TB with limited treatment options. Here, the authors perform RNA sequencing on whole blood and on ventricular and lumbar cerebrospinal fluid (CSF) samples from pediatric patients treated for TBM to characterize the immune response and tissue damage.
- Ursula K. Rohlwink
- , Anthony Figaji
- & Rachel P. J. Lai
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Article
| Open AccessRepeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques
High frequency semen exposure has been associated with activation of anti-HIV mechanisms in HIV negative sex workers. Here, Abdulhaqq et al. show that repeated vaginal exposure to semen reduces vaginal infection by SIV in non-human primates, and is associated with lower CCR5 expression in CD4 T-cells and a local type-I interferon response.
- Shaheed A. Abdulhaqq
- , Melween Martinez
- & Luis J. Montaner
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Article
| Open AccessMapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
The authors introduce ACT-seq: a Tn5-based method for rapidly profiling epigenetic marks in bulk-cell and single-cell samples. ACT-seq avoids many laborious or time-consuming steps required for similar techniques including chromatin fragmentation and end repair.
- Benjamin Carter
- , Wai Lim Ku
- & Keji Zhao
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Article
| Open AccessVisualizing structural transitions of ligand-dependent gating of the TRPM2 channel
The transient receptor potential channel member 2 (TRPM2) ion channel has a function in redox-dependent signaling. Here the authors present the cryo-EM structures of zebrafish TRPM2 in the ligand-free form, with Ca2+ and both ADP-ribose/Ca2+ and observe two-fold symmetric quaternary structure rearrangements in the ligand-bound structures that likely represent intermediate gating states.
- Ying Yin
- , Mengyu Wu
- & Seok-Yong Lee
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Article
| Open AccessA metabolic profile of all-cause mortality risk identified in an observational study of 44,168 individuals
Biomarkers that predict mortality are of interest for clinical as well as research applications. Here, the authors analyze metabolomics data from 44,168 individuals and identify key metabolites independently associated with all-cause mortality risk.
- Joris Deelen
- , Johannes Kettunen
- & P. Eline Slagboom
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Article
| Open AccessMitogenic and progenitor gene programmes in single pilocytic astrocytoma cells
Pilocytic astrocytoma is a low-grade pediatric glioma, characterized by a single BRAF rearrangement. Here, Reitman and colleagues use single-cell RNA sequencing to reveal molecular hallmarks of the disease that might be targeted therapeutically.
- Zachary J. Reitman
- , Brenton R. Paolella
- & Rameen Beroukhim
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Article
| Open AccessElectrical pulse-induced electrochemical biosensor for hepatitis E virus detection
Detection of viral biomarkers is important for disease treatment and prevention. Here, the authors report on a system that uses an electrical pulse-induced electrochemical sensor for the detection of hepatitis E virus, and demonstrate potential application of the device.
- Ankan Dutta Chowdhury
- , Kenshin Takemura
- & Enoch Y. Park
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Article
| Open AccessMICU1 controls cristae junction and spatially anchors mitochondrial Ca2+ uniporter complex
Proper mitochondrial structure is critical for normal function. Here, the authors show with SIM that the mitochondrial Ca2+ uniporter complex and localization of component MICU1 is critical to maintaining cristae junction stability and overall mitochondrial membrane structure.
- Benjamin Gottschalk
- , Christiane Klec
- & Wolfgang F. Graier
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Article
| Open Access3D sub-diffraction imaging in a conventional confocal configuration by exploiting super-linear emitters
Super-resolution microscopy is a valuable tool in bioimaging, but often requires complex systems or post-processing. Here, the authors present super-linear excitation-emission (SEE) microscopy, which overcomes these limitations by taking advantage of markers with super-linear dependence between emission and excitation power.
- Denitza Denkova
- , Martin Ploschner
- & James A. Piper
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Article
| Open AccessDeep multiomics profiling of brain tumors identifies signaling networks downstream of cancer driver genes
Multi-omic profiling is a powerful approach to dissecting molecular mechanisms in disease. Here the authors generate whole proteome, phosphoproteome and transcriptome profiles from two mouse models of high-grade glioma driven by different oncogenes, and validate identified master regulators with a CRISPR screen.
- Hong Wang
- , Alexander K. Diaz
- & Junmin Peng
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Article
| Open AccessAn alternative framework for fluorescence correlation spectroscopy
Fluorescence correlation spectroscopy is widely used for in vivo and in vitro applications, yet extracting information from experiments still requires long acquisition times. Here, the authors exploit Bayesian non-parametrics to directly analyze the output of confocal fluorescence experiments thereby probing physical processes on much faster timescales.
- Sina Jazani
- , Ioannis Sgouralis
- & Steve Pressé
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Article
| Open AccessAn anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters
Anaerobic bacteria represent a rich source of biological and chemical diversity but are difficult to cultivate and there is a lack of heterologous expression systems. Here the authors develop an expression system based on S. mutans UA159 for biosynthetic gene clusters from anaerobic bacteria.
- Tingting Hao
- , Zhoujie Xie
- & Yihua Chen
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Article
| Open AccessA glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier
The glomerular filtration barrier is a complex structure in charge of renal ultrafiltration. Here the authors present a glomerulus-on-a-chip for disease modelling and high-throughput drug screening where human podocytes and human glomerular endothelial cells are separated by an extracellular matrix resembling the in vivo basement membrane.
- Astgik Petrosyan
- , Paolo Cravedi
- & Stefano Da Sacco
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Article
| Open AccessBand-collision gel electrophoresis
Electrophoretic mobility shift assays are widely used in gel electrophoresis to study binding interactions between different molecular species, but these assays access only a subset of reaction possibilities. Here, the authors develop a band-collision gel electrophoresis (BCGE) approach that demonstrates a much wider variety of reaction types.
- Dimitri A. Bikos
- & Thomas G. Mason
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Article
| Open AccessRobust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
Present understanding of Plasmodium vivax biology is hampered by its inability to grow in vitro. Here, the authors developed an in vitro culture of its simian counterpart, P. cynomolgi, which shares morphological and phenotypic similarities with P. vivax, initiating a new phase in vivax research.
- Adeline C. Y. Chua
- , Jessica Jie Ying Ong
- & Pablo Bifani
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Article
| Open AccessA conserved RNA structural motif for organizing topology within picornaviral internal ribosome entry sites
Picornaviruses use modular RNA domains in their internal ribosome entry sites (IRESs) for translation through non-canonical, cap-independent mechanisms. Here the authors report the crystal structure of domain V from the IRES of hepatitis A virus (HAV) ssRNA genome, suggesting that the functional homology among different types of picornaviral IRESs is structure-based.
- Deepak Koirala
- , Yaming Shao
- & Joseph A. Piccirilli
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Article
| Open AccessExpanding C–T base editing toolkit with diversified cytidine deaminases
Cytosine base editors are limited by editing scope and potential off-target effects. Here the authors screen diversified lamprey cytidine deaminases along with different protein fusion architectures and present base editors with improved fidelity.
- Tian-Lin Cheng
- , Shuo Li
- & Zilong Qiu
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Article
| Open AccessProteogenomic landscape of squamous cell lung cancer
Squamous cell lung cancer has dismal prognosis due to the dearth of effective treatments. Here, the authors perform an integrated proteogenomic analysis of the disease, revealing three proteomics-based subtypes and suggesting potential therapeutic opportunities.
- Paul A. Stewart
- , Eric A. Welsh
- & Eric B. Haura
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Article
| Open AccessA consensus S. cerevisiae metabolic model Yeast8 and its ecosystem for comprehensively probing cellular metabolism
Genome-scale metabolic models provide a platform to study metabolism through simulations and analysis of omics data. Here the authors introduce Yeast8 with its model ecosystem, a comprehensive computational resource for simulating the metabolism of Saccharomyces cerevisiae.
- Hongzhong Lu
- , Feiran Li
- & Jens Nielsen
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Article
| Open AccessUltra-sensitive digital quantification of proteins and mRNA in single cells
Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimulation, and dPLA in a smaller reaction volume.
- Jing Lin
- , Christian Jordi
- & Savaş Tay
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Article
| Open AccessHaploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome
Understanding of the genetic factors and molecular mechanisms underlying neurodevelopmental disorders remains incomplete. In this study, authors show that microdeletions in the gene ANKS1B lead to loss of the neuronal synapse-enriched protein AIDA-1 and to a novel neurodevelopmental syndrome
- Abigail U. Carbonell
- , Chang Hoon Cho
- & Bryen A. Jordan
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Article
| Open AccessBrainstem and spinal cord MRI identifies altered sensorimotor pathways post-stroke
There are few studies of structural changes in ascending and descending sensorimotor pathways after stroke, beyond the corticospinal tract, in the brain. Here the authors identify changes in white matter structure in brainstem and spinal cord following stroke, and show its relationship to motor impairment.
- Haleh Karbasforoushan
- , Julien Cohen-Adad
- & Julius P. A. Dewald
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Article
| Open AccessMYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer
The expression of oncogenic MYC paralogs in small cell lung cancer is mutually exclusive. In this study, the authors show that MYC, but not MYCN or MYCL, represses BCL2, resulting in cells that are uniquely sensitive to apoptosis, and find that CHK1 and AURKA inhibitors may be useful for treating these cancers.
- Marcel A. Dammert
- , Johannes Brägelmann
- & Martin L. Sos
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Article
| Open AccessDegron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures
Visualising certain organelles and their dynamics is challenging in living cells. Here the authors co-opt selective degradation to label membrane-bound compartments in worm embryos and mammalian cells, revealing membrane topology during cell division.
- Katharina B. Beer
- , Gholamreza Fazeli
- & Ann M. Wehman
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Article
| Open AccessThe autism- and schizophrenia-associated protein CYFIP1 regulates bilateral brain connectivity and behaviour
In humans, copy-number variants of the CYFIP1 gene have been associated with autism spectrum disorders and schizophrenia. Here, the authors characterize Cyfip1-heterozygous mice, revealing that they display deficits in brain white matter structure and functional connectivity along with abnormal behaviours.
- Nuria Domínguez-Iturza
- , Adrian C. Lo
- & Claudia Bagni
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Article
| Open AccessCyfip1 haploinsufficient rats show white matter changes, myelin thinning, abnormal oligodendrocytes and behavioural inflexibility
People with a genetic deletion of the 15q11.2 locus are at increased risk for psychiatric disorders and white matter disturbances, but the gene(s) responsible are unclear. Here, the authors show that low dosage of CYFIP1, present in the human 15q11.2 region, alters white matter structure and cognition in rats.
- Ana I. Silva
- , Josephine E. Haddon
- & Lawrence S. Wilkinson
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Article
| Open AccessCell-type-specific resolution epigenetics without the need for cell sorting or single-cell biology
Compared to bulk data, cell-type-specific DNA methylation data provide higher resolution of epigenetic variation. Here, the authors introduce Tensor Composition Analysis, a novel computational approach for learning cell-type-specific DNA methylation from tissue-level bulk data, and show its application in epigenome-wide association studies.
- Elior Rahmani
- , Regev Schweiger
- & Eran Halperin
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Article
| Open AccessA comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes
The Oxford Nanopore system is capable of direct RNA sequencing but complex transcriptome analysis still remains to be thoroughly investigated. Here the authors perform sequencing of native RNA as well as cDNA to characterise the strengths and weaknesses of the system.
- Charlotte Soneson
- , Yao Yao
- & Shobbir Hussain
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Article
| Open AccessThe LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins
Atherosclerosis results from the accumulation of lipoproteins in the vascular wall. Here, Thierer et al. report the design of a chemiluminescent reporter for atherogenic lipoproteins using fusion of apolipoprotein-B to a luciferase enzyme, and find it bears potential for the identification of regulators of lipoprotein metabolism in vivo.
- James H. Thierer
- , Stephen C. Ekker
- & Steven A. Farber
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Article
| Open AccessA high-speed search engine pLink 2 with systematic evaluation for proteome-scale identification of cross-linked peptides
The identification of cross-linked peptides at a proteome scale for interactome analyses represents a complex challenge. Here the authors report an efficient and reliable search engine pLink 2 for proteome-scale cross-linking mass spectrometry analyses, and demonstrate how to systematically evaluate the credibility of search engines.
- Zhen-Lin Chen
- , Jia-Ming Meng
- & Si-Min He
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Article
| Open AccessSingle-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
Single-molecule localization microscopy (SMLM) requires the use of fluorophores with specific sets of properties. Here the authors employ conventional BODIPY dyes as SMLM fluorophores by making use of rarely reported red-shifted ground state BODIPY dimers to image fatty acids, lipid droplets and lysosomes at single-molecule resolution.
- Santosh Adhikari
- , Joe Moscatelli
- & Elias M. Puchner
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Article
| Open AccessStimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
Manipulating DNA repair pathways can be used to improve the outcomes of CRISPR-based genome editing. Here the authors derive an enhanced RAD18 variant that suppresses 53BP1 recruitment to DNA double-strand breaks to enhance homology-mediated repair.
- Tarun S. Nambiar
- , Pierre Billon
- & Alberto Ciccia
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Article
| Open AccessPolarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
Many developing tissues require Par-driven polarization, but its role in mammalian tissue maintenance is unclear. Here, the authors show that in mouse epidermis, Par3 governs tissue homeostasis not via orientation of cell division but by coupling cell mechanics with mitotic accuracy and genome integrity.
- Martim Dias Gomes
- , Soriba Letzian
- & Sandra Iden
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Article
| Open AccessTargeting metabolic driving and intermediate influx in lysine catabolism for high-level glutarate production
The efficiency of producing glutarate, a valuable platform C5 compound, by engineered E. coli is low. Here, the authors achieve high titer and yield of glutarate production by pushing and pulling carbon flux in the native pathway and increasing intermediate influx using newly identified transporters.
- Wenna Li
- , Lin Ma
- & Qipeng Yuan
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Article
| Open AccessArabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition
The SWI2/SNF2-Related 1 chromatin remodeling complex (SWR1-C) is important for gene regulation, but its composition remains largely uncharacterized in plants. Here, the authors report that methyl-CpG-binding domain 9 (MBD9) is a SWR1-C interacting protein required for histone H2A.Z deposition in Arabidopsis.
- Magdalena E. Potok
- , Yafei Wang
- & Steven E. Jacobsen
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Article
| Open AccessSingle cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors
The liver parenchyma consists of several cell types, but the origin of this tissue in humans is unclear. Here, the authors perform single cell RNA sequencing of human fetal and adult liver to identify a hepatobiliary hybrid progenitor population of cells, which have a similar gene signature to mouse oval cells.
- Joe M. Segal
- , Deniz Kent
- & S. Tamir Rashid
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Article
| Open AccessKLF-1 orchestrates a xenobiotic detoxification program essential for longevity of mitochondrial mutants
Cytochrome P450 oxidases (CYPs) are enzymes that participate in the xenobiotic detoxification and their expression is enhanced in long-lived model organisms. Here the authors show that KLF-1 promotes cyp expression and ensures lifespan extension in C. elegans mitomutants by activating mitohormesis.
- Marija Herholz
- , Estela Cepeda
- & Aleksandra Trifunovic
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Article
| Open AccessA standard for near-scarless plasmid construction using reusable DNA parts
Construction of plasmids from multiple fragments often uses customised parts and leaves scars where fragments are joined. Here the authors develop a method for barcoding fragments and constructing plasmids in a scarless manner from a collection of standard parts.
- Xiaoqiang Ma
- , Hong Liang
- & Kang Zhou
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Article
| Open AccessAugmentation of myocardial If dysregulates calcium homeostasis and causes adverse cardiac remodeling
The depolarizing funny current contributing to cardiac pacemaking is upregulated in the myocardium of failing and infarcted hearts, but whether the current is implied in disease mechanisms is unclear. Here the authors generate HCN4 transgenic mice and show that upregulation of funny current to the levels observed in human heart failure alters calcium homeostasis leading to cardiac remodelling and arrhythmia.
- Pessah Yampolsky
- , Michael Koenen
- & Patrick A. Schweizer
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Review Article
| Open AccessTowards a standardized bioinformatics infrastructure for N- and O-glycomics
Glycomics is gaining momentum in basic, translational and clinical research. Here, the authors review current reporting standards and analysis tools for mass-spectrometry-based glycomics, and propose an e-infrastructure for standardized reporting and online deposition of glycomics data.
- Miguel A. Rojas-Macias
- , Julien Mariethoz
- & Niclas G. Karlsson
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Article
| Open AccessPredicting bacterial infection outcomes using single cell RNA-sequencing analysis of human immune cells
Complex interactions between different host immune cell types can determine the outcome of pathogen infections. Here, Avraham and colleagues present a deconvolution algorithm that uses single-cell RNA and bulk RNA sequencing measurements of pathogen-infected cells to predict disease risk outcomes.
- Noa Bossel Ben-Moshe
- , Shelly Hen-Avivi
- & Roi Avraham
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Article
| Open AccessTurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
Plant NLR receptors trigger immune signaling following recognition of pathogen effectors. Here, Zhang et al. optimize a TurboID-based proximity labeling approach and show that it can be used to identify interacting partners of N, an NLR that confers resistance to Tobacco mosaic virus.
- Yongliang Zhang
- , Gaoyuan Song
- & Savithramma P. Dinesh-Kumar
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Article
| Open AccessSubtype-specific secretomic characterization of pulmonary neuroendocrine tumor cells
Secreted proteins present a rich resource of potential cancer biomarkers. Here, the authors use mass spectrometry to analyze secretome remodeling in pulmonary neuroendocrine lung cancer cell lines and validate potential biomarkers and therapeutic targets in vitro and in mouse models.
- Xu-Dong Wang
- , Rongkuan Hu
- & Yonghao Yu
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Article
| Open AccessQuantum biological tunnel junction for electron transfer imaging in live cells
Although quantum biological electron transfer is important in many biological processes, imaging of the events in live cells has remained challenging. Here, the authors demonstrate real-time optical detection of quantum biological electron tunnelling between nanoparticles and cytochrome c inside living cells.
- Hongbao Xin
- , Wen Jing Sim
- & Luke P. Lee
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Article
| Open AccessLive cell imaging reveals 3′-UTR dependent mRNA sorting to synapses
Asymmetric subcellular mRNA distribution is important for local translation of neuronal mRNAs. Here the authors employed MS2 live-cell imaging and showed that the reporter mRNA containing the 3’ UTR of Rgs4 shows an anterograde transport bias, dependent on neuronal activity and the protein Staufen2, and mediates sustained mRNA recruitment to synapses.
- Karl E. Bauer
- , Inmaculada Segura
- & Michael A. Kiebler
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